PLNT3140 INTRODUCTORY CYTOGENETICS
Exercise: CHROMATIN PACKING
1) Given the data listed below, calculate
a) The physical length of the
Vicia faba
genome
b) The packing ratio of the
Vicia faba
genome (ratio of DNA length to chromosome length).
c) The packing ratio of a spool of thread.
Typical eukaryotic nucleus: 3-10 µm in diameter
Combined length of all
Vicia faba
metaphase (root tip)
chromosomes: 0.112 mm
Vicia faba
diploid genome size: 14.6 pg= 1.33 x 10
10
bp
1 helical turn = 10 bp = 3.4 nm
Typical spool of thread: 4.5 cm long, contains 184 M of thread.
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