ID MYG_HUMAN Reviewed; 154 AA.
AC P02144; Q52H51; Q5THY7;
DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 2.
DT 23-JAN-2007, entry version 74.
DE Myoglobin.
GN Name=MB;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC Catarrhini; Hominidae; Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX MEDLINE=84182508; PubMed=6571704;
RA Weller P., Jeffreys A.J., Wilson V., Blanchetot A.;
RT "Organization of the human myoglobin gene.";
RL EMBO J. 3:439-446(1984).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX MEDLINE=85232026; PubMed=2989088; DOI=10.1016/0378-1119(85)90231-8;
RA Akaboshi E.;
RT "Cloning of the human myoglobin gene.";
RL Gene 33:241-249(1985).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15461802; DOI=10.1186/gb-2004-5-10-r84;
RA Collins J.E., Wright C.L., Edwards C.A., Davis M.P., Grinham J.A.,
RA Cole C.G., Goward M.E., Aguado B., Mallya M., Mokrab Y., Huckle E.J.,
RA Beare D.M., Dunham I.;
RT "A genome annotation-driven approach to cloning the human ORFeome.";
RL Genome Biol. 5:RESEARCH84.1-RESEARCH84.11(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S.,
RA Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y.,
RA Phelan M., Farmer A.;
RT "Cloning of human full-length CDSs in BD Creator(TM) system donor
RT vector.";
RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Livingston R.J., Rieder M.J., Shaffer T., Bertucci C., Baier C.N.,
RA Rajkumar N., Willa H.T., Daniels M., Downing T.K., Stanaway I.B.,
RA Nguyen C.P., Gildersleeve H., Cassidy C.M., Johnson E.J.,
RA Swanson J.E., McFarland I., Yool B., Park C., Nickerson D.A.;
RT "NIEHS-SNPs, environmental genome project, NIEHS ES15478, Department
RT of Genome Sciences, Seattle, WA (URL: http://egp.gs.washington.edu).";
RL Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX MEDLINE=20057165; PubMed=10591208; DOI=10.1038/990031;
RA Dunham I., Hunt A.R., Collins J.E., Bruskiewich R., Beare D.M.,
RA Clamp M., Smink L.J., Ainscough R., Almeida J.P., Babbage A.K.,
RA Bagguley C., Bailey J., Barlow K.F., Bates K.N., Beasley O.P.,
RA Bird C.P., Blakey S.E., Bridgeman A.M., Buck D., Burgess J.,
RA Burrill W.D., Burton J., Carder C., Carter N.P., Chen Y., Clark G.,
RA Clegg S.M., Cobley V.E., Cole C.G., Collier R.E., Connor R.,
RA Conroy D., Corby N.R., Coville G.J., Cox A.V., Davis J., Dawson E.,
RA Dhami P.D., Dockree C., Dodsworth S.J., Durbin R.M., Ellington A.G.,
RA Evans K.L., Fey J.M., Fleming K., French L., Garner A.A.,
RA Gilbert J.G.R., Goward M.E., Grafham D.V., Griffiths M.N.D., Hall C.,
RA Hall R.E., Hall-Tamlyn G., Heathcott R.W., Ho S., Holmes S.,
RA Hunt S.E., Jones M.C., Kershaw J., Kimberley A.M., King A.,
RA Laird G.K., Langford C.F., Leversha M.A., Lloyd C., Lloyd D.M.,
RA Martyn I.D., Mashreghi-Mohammadi M., Matthews L.H., Mccann O.T.,
RA Mcclay J., Mclaren S., McMurray A.A., Milne S.A., Mortimore B.J.,
RA Odell C.N., Pavitt R., Pearce A.V., Pearson D., Phillimore B.J.C.T.,
RA Phillips S.H., Plumb R.W., Ramsay H., Ramsey Y., Rogers L., Ross M.T.,
RA Scott C.E., Sehra H.K., Skuce C.D., Smalley S., Smith M.L.,
RA Soderlund C., Spragon L., Steward C.A., Sulston J.E., Swann R.M.,
RA Vaudin M., Wall M., Wallis J.M., Whiteley M.N., Willey D.L.,
RA Williams L., Williams S.A., Williamson H., Wilmer T.E., Wilming L.,
RA Wright C.L., Hubbard T., Bentley D.R., Beck S., Rogers J., Shimizu N.,
RA Minoshima S., Kawasaki K., Sasaki T., Asakawa S., Kudoh J.,
RA Shintani A., Shibuya K., Yoshizaki Y., Aoki N., Mitsuyama S.,
RA Roe B.A., Chen F., Chu L., Crabtree J., Deschamps S., Do A., Do T.,
RA Dorman A., Fang F., Fu Y., Hu P., Hua A., Kenton S., Lai H., Lao H.I.,
RA Lewis J., Lewis S., Lin S.-P., Loh P., Malaj E., Nguyen T., Pan H.,
RA Phan S., Qi S., Qian Y., Ray L., Ren Q., Shaull S., Sloan D., Song L.,
RA Wang Q., Wang Y., Wang Z., White J., Willingham D., Wu H., Yao Z.,
RA Zhan M., Zhang G., Chissoe S., Murray J., Miller N., Minx P.,
RA Fulton R., Johnson D., Bemis G., Bentley D., Bradshaw H., Bourne S.,
RA Cordes M., Du Z., Fulton L., Goela D., Graves T., Hawkins J.,
RA Hinds K., Kemp K., Latreille P., Layman D., Ozersky P., Rohlfing T.,
RA Scheet P., Walker C., Wamsley A., Wohldmann P., Pepin K., Nelson J.,
RA Korf I., Bedell J.A., Hillier L.W., Mardis E., Waterston R.,
RA Wilson R., Emanuel B.S., Shaikh T., Kurahashi H., Saitta S.,
RA Budarf M.L., McDermid H.E., Johnson A., Wong A.C.C., Morrow B.E.,
RA Edelmann L., Kim U.J., Shizuya H., Simon M.I., Dumanski J.P.,
RA Peyrard M., Kedra D., Seroussi E., Fransson I., Tapia I., Bruder C.E.,
RA O'Brien K.P., Wilkinson P., Bodenteich A., Hartman K., Hu X.,
RA Khan A.S., Lane L., Tilahun Y., Wright H.;
RT "The DNA sequence of human chromosome 22.";
RL Nature 402:489-495(1999).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Skeletal muscle;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA
RT project: the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [8]
RP PROTEIN SEQUENCE OF 2-154.
RX MEDLINE=71291923; PubMed=5285572;
RA Romero-Herrera A.E., Lehmann H.;
RT "Primary structure of human myoglobin.";
RL Nature New Biol. 232:149-152(1971).
RN [9]
RP SEQUENCE REVISION TO 20-23 AND 84.
RX PubMed=11452891;
RA Romero-Herrera A.E., Lehmann H.;
RT "The myoglobin of primates. I. Hylobates agilis (gibbon).";
RL Biochim. Biophys. Acta 251:482-488(1971).
RN [10]
RP SEQUENCE REVISION TO 100-102.
RX MEDLINE=73002055; PubMed=4627044;
RA Romero-Herrera A.E., Lehmann H.;
RT "The myoglobin of primates. II. Pan troglodytes (chimpanzee).";
RL Biochim. Biophys. Acta 278:62-67(1972).
RN [11]
RP PROTEIN SEQUENCE OF 2-21.
RC TISSUE=Heart;
RX MEDLINE=95203287; PubMed=7895732;
RA Corbett J.M., Wheeler C.H., Baker C.S., Yacoub M.H., Dunn M.J.;
RT "The human myocardial two-dimensional gel protein database: update
RT 1994.";
RL Electrophoresis 15:1459-1465(1994).
RN [12]
RP VARIANT LYS-55.
RX MEDLINE=69275845; PubMed=5805522;
RA Boulton F.E., Huntsman R.G., Lorkin P.A., Lehmann H.;
RT "Abnormal human myoglobin: 53 (D4) glutamic acid-->lysine.";
RL Nature 223:832-833(1969).
RN [13]
RP VARIANT TRP-140.
RX MEDLINE=71177026; PubMed=5555219;
RA Boulton F.E., Huntsman R.G., Romero Herrera A., Lorkin P.A.,
RA Lehmann H.;
RT "The third variant of human myoglobin showing an unusual amino acid
RT substitution: 138(H16)arginine-->tryptophan.";
RL Biochim. Biophys. Acta 229:716-719(1971).
RN [14]
RP VARIANT ASN-134.
RX MEDLINE=71177044; PubMed=5555226;
RA Boulton F.E., Huntsman R.G., Romero Herrera A.E., Lorkin P.A.,
RA Lehmann H.;
RT "A human myoglobin variant 133 (H-10)lysine-->asparagine.";
RL Biochim. Biophys. Acta 229:871-876(1971).
RN [15]
RP VARIANT GLN-140.
RX MEDLINE=71085676; PubMed=5540041;
RA Boulton F.E., Huntsman R.G., Yawson G.I., Romero-Herrera A.E.,
RA Lorkin P.A.;
RT "The second variant of human myoglobin; 138(H16) arginine leads to
RT glutamine.";
RL Br. J. Haematol. 20:69-74(1971).
RN [16]
RP X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF MUTANT WITH ARG-46 AND
RP ALA-111.
RX MEDLINE=90258028; PubMed=2342104;
RA Hubbard S.R., Hendrickson W.A., Lambright D.G., Boxer S.G.;
RT "X-ray crystal structure of a recombinant human myoglobin mutant at
RT 2.8-A resolution.";
RL J. Mol. Biol. 213:215-218(1990).
CC -!- FUNCTION: Serves as a reserve supply of oxygen and facilitates the
CC movement of oxygen within muscles.
CC -!- SIMILARITY: Belongs to the globin family.
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DR EMBL; X00371; CAA25109.1; -; Genomic_DNA.
DR EMBL; X00372; CAA25109.1; JOINED; Genomic_DNA.
DR EMBL; X00373; CAA25109.1; JOINED; Genomic_DNA.
DR EMBL; M14603; AAA59595.1; -; Genomic_DNA.
DR EMBL; M10090; AAA59595.1; JOINED; Genomic_DNA.
DR EMBL; M14602; AAA59595.1; JOINED; Genomic_DNA.
DR EMBL; CR456516; CAG30402.1; -; mRNA.
DR EMBL; CR541949; CAG46747.1; -; mRNA.
DR EMBL; DQ003030; AAX84516.1; -; Genomic_DNA.
DR EMBL; AL022334; CAI21837.1; -; Genomic_DNA.
DR EMBL; AL049747; CAI21837.1; JOINED; Genomic_DNA.
DR EMBL; AL049747; CAI23587.1; -; Genomic_DNA.
DR EMBL; AL022334; CAI23587.1; JOINED; Genomic_DNA.
DR EMBL; BC014547; AAH14547.1; -; mRNA.
DR PIR; I53991; MYHU.
DR UniGene; Hs.517586; -.
DR PDB; 2MM1; X-ray; @=1-154.
DR HSC-2DPAGE; P02144; HUMAN.
DR Ensembl; ENSG00000198125; Homo sapiens.
DR KEGG; hsa:4151; -.
DR H-InvDB; HIX0016418; -.
DR HGNC; HGNC:6915; MB.
DR HPA; CAB000060; -.
DR HPA; HPA003123; -.
DR MIM; 160000; gene.
DR LinkHub; P02144; -.
DR ArrayExpress; P02144; -.
DR GermOnline; ENSG00000198125; Homo sapiens.
DR RZPD-ProtExp; IOH12916; -.
DR RZPD-ProtExp; RZPDo834C1234; -.
DR RZPD-ProtExp; S0336; -.
DR RZPD-ProtExp; T2778; -.
DR InterPro; IPR000971; Globin.
DR InterPro; IPR009050; Globin_like.
DR InterPro; IPR012292; Globin_related.
DR InterPro; IPR002335; Myoglobin.
DR Gene3D; G3DSA:1.10.490.10; Globin_related; 1.
DR Pfam; PF00042; Globin; 1.
DR PRINTS; PR00613; MYOGLOBIN.
DR PROSITE; PS01033; GLOBIN; 1.
KW 3D-structure; Direct protein sequencing; Heme; Iron; Metal-binding;
KW Muscle protein; Oxygen transport; Polymorphism; Transport.
FT INIT_MET 1 1 Removed.
FT CHAIN 2 154 Myoglobin.
FT /FTId=PRO_0000053303.
FT METAL 65 65 Iron (heme distal ligand).
FT METAL 94 94 Iron (heme proximal ligand).
FT VARIANT 55 55 E -> K.
FT /FTId=VAR_003180.
FT VARIANT 134 134 K -> N.
FT /FTId=VAR_003181.
FT VARIANT 140 140 R -> Q.
FT /FTId=VAR_003182.
FT VARIANT 140 140 R -> W.
FT /FTId=VAR_003183.
FT CONFLICT 106 106 E -> Q (in Ref. 5).
FT CONFLICT 129 129 Q -> E (in Ref. 2).
FT HELIX 5 18
FT TURN 19 20
FT HELIX 22 36
FT HELIX 38 42
FT TURN 43 43
FT TURN 46 49
FT HELIX 53 58
FT HELIX 60 77
FT TURN 78 81
FT HELIX 84 96
FT TURN 97 97
FT TURN 102 102
FT HELIX 103 119
FT HELIX 121 123
FT HELIX 126 149
FT TURN 150 151
SQ SEQUENCE 154 AA; 17184 MW; F6A41F19A525F09C CRC64;
MGLSDGEWQL VLNVWGKVEA DIPGHGQEVL IRLFKGHPET LEKFDKFKHL KSEDEMKASE
DLKKHGATVL TALGGILKKK GHHEAEIKPL AQSHATKHKI PVKYLEFISE CIIQVLQSKH
PGDFGADAQG AMNKALELFR KDMASNYKEL GFQG
//