DRR206 IS ACTIVATED WITH RACE-SPECIFICITY BY BACTERIA AND FUNGI

The DRR206 gene family encodes a protein of approx. 20 kDa with no significant similarity to any known protein.

 garnier plot of gde22861_2.seq, 184 aa; DCH = 0, DCS = 0
 >DRR206C
           .   10    .   20    .   30    .   40    .   50    .   60
       MGSKLPVLFVFVMLFALSSAIPNKRKPYKPCKNLVLYFHDILYNGKNAANATSAIVAAPE
 helix            HHHHHHHHH                            HHHHH    HHH
 sheet      EEEEEE                 E  EEEEEEEEEEE           EEEE   
 turns TTT                   TTTTTT TT           TTT               
 coil     CC               CC                       CCC            

           .   70    .   80    .   90    .  100    .  110    .  120
       GVSLTKLAPQSHFGNIIVFDDPITLSHSLSSKQVGRAQGFYIYDTKNTYTSWLSFTFVLN
 helix HHHHHH                 HH                                   
 sheet         E     EEEEEE             EEEEEEEEEE        EEEEE    
 turns          T TTT       TT       TTT          TTTTTTTT         
 coil        CC  C         C    CCCCC                          CCCC

           .  130    .  140    .  150    .  160    .  170    .  180
       STHHQGTITFAGADPIVAKTRDISVTGGTGDFFMHRGIATITTDAFEGEAYFRLGVYIKF
 helix               HHH                            HHHHHHHHH      
 sheet       EEEE       EEEEEEEE      EEE     EEE               EEE
 turns     TT                                                TTT   
 coil  CCCC      CCCC           CCCCCC   CCCCC   CCC               

       
       FECW
 helix  H  
 sheet E   
 turns   TT
 coil      

 Residue totals: H: 38   E: 66   T: 38   C: 42
        percent: H: 22.6 E: 39.3 T: 22.6 C: 25.0

DRR206 is strongly induced by Fusarium oxysporum in seedlings of near isogenic pea lines Vantage (R) and M410 (S).

Hadwiger et al. (1992) Phys. Mol. Plant Pathol. 40:259-269.

DRR206 genes are most strongly-expressed against incompatible races of Pseudomonas syringae pv. pisi.

Daniels et al., (1987) Plant Mol. Biol. 8:309-316.
Pea cultivar P. syringae race Infection phenotype Time period
10h 20h 30h
Mars R-1 S 1.52 1.46 0.92
R-2 R 1.86 4.95 1.98
R-3 S 2.26 1.13 1.61
Abador R-1 R 2.10 5.11 2.01
R-2 R 1.98 3.41 2.05
R-3 S 1.33 3.43 2.12
Ceras R-1 R 4.23 3.37 2.15
R-2 S 1.72 2.33 1.51
R-3 S 1.56 2.09 0.75
Mini R-1 R 2.85 4.77 3.60
R-2 S 1.54 2.45 2.71
R-3 S 2.31 2.89 3.23
Spring R-1 S 1.12 1.91 1.19
R-2 R 0.81 4.64 1.47
R-3 R 0.38 2.02 1.12
Signal from Northern blots expressed as a fraction of water control.
 

 DRR206 is also induced by lipoprotein-peptidoglycan preparations, or killed cells, from P. syringae in a non-race-specific fashion.

Daniels et al., (1988) Phytopathology 78: 1451-1453.