Where do we go from here?

Gene expression studies using EST arrays

• new coding sequences, novel genes
• gene array studies on expression - leads to promoter identification
• comparison of different types of defense responses
• fungi vs. bacteria
• specific resistance vs. basic resistance

Are there different kinds of basic resistance?
• systemic effects on gene expression

Mechanism of DRR206 action

• EST arrays to compare transformed vs. untransformed
• microscopy
• bioassays
• biochemistry

Which of the following pathogens are controlled by DRR206?

• L. maculans/P. lingam
• S. sclerotiorum
• Rhizoctonia solani
• Alternaria brassicae (pod spot)

Through different combinations of small array of coding sequences and promoters, we can generate a diverse range of resistant genotypes.  A strategy of genetic engineering based on diversity may allow us to develop new resistance phenotypes more rapidly than pathogen populations can over come them.