# tfastx3 -T 32 -Q -p -b %NUMOFSCORES% -d 20 -m 0 -z 1 -E 1.0e-5 -m "F8C bio8144906429648998462.tmp.tsv" -m "F6 bio8144906429648998462.tmp.html" bio8144906429648998462.tmp @/home/psgendb/BIRCHDEV/dat/fasta/nt.nam 3
TFASTX compares a protein to a translated DNA data bank
 version 36.3.8h Aug, 2019
Please cite:
 Pearson et al, Genomics (1997) 46:24-36

Query: bio8144906429648998462.tmp
  1>>>SRAAFP:CDS1 - 196 aa
Library: @/home/psgendb/BIRCHDEV/dat/fasta/nt.nam
  242370672807 residues in 54289499 sequences
Statistics:  Expectation_n fit: rho(ln(x))= 5.1512+/-0.00014; mu= 9.5732+/- 0.009
 mean_var=71.5504+/-13.032, 0's: 13 Z-trim(112.4): 141  B-trim: 1825 in 2/94
 Lambda= 0.151624
 statistics sampled from 60000 (117185) to 8970211 sequences
Algorithm: TFASTX (3.8 June 2014) [optimized]
Parameters: BL50 matrix (15:-5), open/ext: -12/-2, shift: -20
 ktup: 3, E-join: 0.5 (0.36), E-opt: 0.1 (0.0889), width:  16
 Scan time: 12525.890

The best scores are:                             initn opt bits E(55167125)
gi|J02593.1|SRAAFP Sea raven (Hemitri ( 874) [f] 1369 1376 313.0 5.7e-82
dbj|AB283044.1| Brachyopsis rostratus ( 507) [f]  793  821 191.4 1.3e-45
gi|EU719616.1| Siniperca chuatsi anti ( 905) [f]  526  625 148.7 1.7e-32
ref|XM_028577120.1| PREDICTED: Perca  (1140) [f]  400  518 125.4 2.2e-25
gi|FJ826540.1| Perca flavescens type  (1218) [f]  400  518 125.4 2.3e-25
gi|M96154.1|OSEAFP Osmerus mordax ant ( 611) [f]  364  512 123.9 3.4e-25
gi|FJ826539.1| Perca flavescens type  ( 859) [f]  404  502 121.8   2e-24
ref|XM_028577118.1| PREDICTED: Perca  ( 927) [f]  404  502 121.8 2.1e-24
ref|XM_028577117.1| PREDICTED: Perca  ( 834) [f]  382  500 121.3 2.7e-24
gi|DQ062448.1| Osmerus mordax clone 1 ( 539) [f]  377  496 120.3 3.5e-24
gi|FJ826541.1| Perca flavescens type  ( 869) [f]  374  493 119.8   8e-24
ref|XM_018680358.1| PREDICTED: Lates  ( 912) [f]  281  483 117.7 3.8e-23
ref|XM_018703530.1| PREDICTED: Lates  ( 652) [f]  281  479 116.7 5.3e-23
ref|XM_018703522.1| PREDICTED: Lates  ( 897) [f]  281  479 116.8 6.8e-23
ref|XM_028564253.1| PREDICTED: Perca  ( 708) [f]  394  476 116.0   9e-23
ref|XM_003457801.5| PREDICTED: Oreoch (1273) [f]  203  479 116.9   9e-23
ref|XM_030429850.1| PREDICTED: Sparus ( 661) [f]  410  475 115.8 9.9e-23
ref|XM_025901702.1| PREDICTED: Oreoch (1726) [f]  249  479 117.0 1.1e-22
+-                                                127  342 87.0 1.2e-13
ref|XM_018680359.1| PREDICTED: Lates  ( 856) [f]  281  474 115.7 1.4e-22
ref|XM_030754005.1| PREDICTED: Archoc ( 702) [f]  301  471 114.9 1.9e-22
ref|XM_013265793.3| PREDICTED: Oreoch (1365) [f]  414  474 115.8   2e-22
gi|EU660936.1| Dicentrarchus labrax C ( 719) [f]  373  470 114.7 2.3e-22
ref|XM_026162507.1| PREDICTED: Astato ( 812) [f]  402  470 114.8 2.5e-22
ref|XM_015021577.1| PREDICTED: Poecil ( 717) [f]  292  468 114.3   3e-22
ref|XM_030429846.1| PREDICTED: Sparus ( 888) [f]  266  468 114.4 3.6e-22
ref|XM_030429847.1| PREDICTED: Sparus ( 900) [f]  266  468 114.4 3.6e-22
ref|XM_030429845.1| PREDICTED: Sparus ( 945) [f]  266  468 114.4 3.8e-22
ref|XM_026162508.1| PREDICTED: Astato ( 891) [f]  397  466 113.9 4.9e-22
ref|XM_015021576.1| PREDICTED: Poecil ( 705) [f]  272  463 113.2 6.4e-22
ref|XM_015024499.1| PREDICTED: Poecil ( 648) [f]  188  462 113.0   7e-22
ref|XM_023399361.1| PREDICTED: Seriol ( 837) [f]  384  463 113.3 7.3e-22
ref|XM_005755315.1| PREDICTED: Pundam (1275) [f]  299  465 113.8 7.5e-22
ref|XM_026162511.1| PREDICTED: Astato ( 804) [f]  393  462 113.0 8.3e-22
ref|XM_026162509.1| PREDICTED: Astato ( 839) [f]  397  461 112.8 9.9e-22
ref|XM_014413924.2| PREDICTED: Maylan ( 815) [f]  391  460 112.6 1.1e-21
ref|XM_024800189.1| PREDICTED: Maylan ( 837) [f]  397  459 112.4 1.3e-21
ref|XM_026353424.1| PREDICTED: Anabas ( 483) [f]  354  455 111.3 1.6e-21
ref|XM_014976232.1| PREDICTED: Poecil ( 728) [f]  220  456 111.7 1.9e-21
ref|XM_012858718.2| PREDICTED: Fundul (1324) [f]  208  459 112.5 1.9e-21
ref|XM_022751368.1| PREDICTED: Seriol (1488) [f]  231  459 112.6 2.1e-21
ref|XM_024800191.1| PREDICTED: Maylan ( 823) [f]  397  454 111.3 2.8e-21
ref|XM_030162186.1| PREDICTED: Sphaer ( 570) [f]  111  452 110.7 2.9e-21
ref|XM_024800190.1| PREDICTED: Maylan ( 847) [f]  397  454 111.3 2.9e-21
ref|XM_030754008.1| PREDICTED: Archoc ( 575) [f]  304  451 110.5 3.4e-21
ref|XM_021314307.1| PREDICTED: Fundul (1325) [f]  208  455 111.6 3.5e-21
ref|XM_003457793.5| PREDICTED: Oreoch (1327) [f]  354  453 111.2 4.8e-21
ref|XM_018703542.1| PREDICTED: Lates  ( 891) [f]  343  449 110.2 6.4e-21
ref|XM_018680360.1| PREDICTED: Lates  ( 915) [f]  343  449 110.2 6.6e-21
ref|XM_012858321.2| PREDICTED: Fundul ( 774) [f]  222  447 109.7 7.8e-21
ref|XM_007542869.2| PREDICTED: Poecil ( 841) [f]  193  447 109.8 8.3e-21

>>>SRAAFP:CDS1, 196 aa vs @/home/psgendb/BIRCHDEV/dat/fasta/nt.nam library
>>gi|J02593.1|SRAAFP Sea raven (Hemitripterus             (874 aa)
Frame: f initn: 1369 init1: 1369 opt: 1376  Z-score: 1643.0  bits: 313.0 E(55167125): 5.7e-82
Smith-Waterman score: 1376; 100.0% identity (100.0% similar) in 196 aa overlap (1-196:10-597)

               10        20        30        40        50        60
SRAAFP MQRQQADTETREDISTAGLSIIFIVCTISTTRMLTVSLLVCAMMALTQANDDKILKGTAT
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
gi|J02 MQRQQADTETREDISTAGLSIIFIVCTISTTRMLTVSLLVCAMMALTQANDDKILKGTAT
      10        40        70       100       130       160         

               70        80        90       100       110       120
SRAAFP EAGPVSQRAPPNCPAGWQPLGDRCIYYETTAMTWALAETNCMKLGGHLASIHSQEEHSFI
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
gi|J02 EAGPVSQRAPPNCPAGWQPLGDRCIYYETTAMTWALAETNCMKLGGHLASIHSQEEHSFI
     190       220       250       280       310       340         

              130       140       150       160       170       180
SRAAFP QTLNAGVVWIGGSACLQAGAWTWSDGTPMNFRSWCSTKPDDVLAACCMQMTAAADQCWDD
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
gi|J02 QTLNAGVVWIGGSACLQAGAWTWSDGTPMNFRSWCSTKPDDVLAACCMQMTAAADQCWDD
     370       400       430       460       490       520         

              190      
SRAAFP LPCPASHKSVCAMTF*
       ::::::::::::::::
gi|J02 LPCPASHKSVCAMTF*
     550       580     


>>dbj|AB283044.1| Brachyopsis rostratus mRN               (507 aa)
Frame: f initn: 793 init1: 644 opt: 821  Z-score: 990.2  bits: 191.4 E(55167125): 1.3e-45
Smith-Waterman score: 821; 68.3% identity (84.1% similar) in 164 aa overlap (33-192:1-492)

             40        50        60            70        80        
SRAAFP MLTVSLLVCAMMALTQANDDKILKGTATEAGPVS----QRAPPNCPAGWQPLGDRCIYYE
       :::::::::::::::::. : .::::::::: ::    .:    :::::   :.::.: :
dbj|AB MLTVSLLVCAMMALTQADHDGVLKGTATEAGEVSPVFRSRRALVCPAGWTLHGQRCFYSE
         10        40        70       100       130       160      

       90       100       110       120       130       140        
SRAAFP TTAMTWALAETNCMKLGGHLASIHSQEEHSFIQTLNAGVVWIGGSACLQAGAWTWSDGTP
       .::::: :::.::.. ::::::::: ::. .:. . ::.::::::::  ::::.:.::::
dbj|AB ATAMTWDLAEANCVNKGGHLASIHSLEEQLYIKDIVAGIVWIGGSACKVAGAWSWTDGTP
        190       220       250       280       310       340      

      150       160       170       180       190  
SRAAFP MNFRSWCSTKPDDVLAACCMQMTAAADQCWDDLPCPASHKSVCA
       ...:.:: :::.:.:. ::::::::.:.::::::::::: :.::
dbj|AB VDYRTWCPTKPNDILSDCCMQMTAAVDKCWDDLPCPASHASICA
        370       400       430       460       490


>>gi|EU719616.1| Siniperca chuatsi antifree               (905 aa)
Frame: f initn: 526 init1: 411 opt: 625  Z-score: 755.0  bits: 148.7 E(55167125): 1.7e-32
Smith-Waterman score: 625; 49.7% identity (73.2% similar) in 179 aa overlap (21-196:28-564)

               30        40        50          60         70       
SRAAFP IIFIVCTISTTRMLTVSLLVCAMMALTQAN--DDKILKGTATEAGPVS-QRAPPNCPAGW
       ::: .  ::::::::::::::::::::.::  .:  ..::.:.   ..   :  .:: ::
gi|EU7 IIFNIYIISTTRMLTVSLLVCAMMALTRANGEEDTSINGTVTNDTNIDVLNARNGCPPGW
          40        70       100       130       160       190     

        80        90       100       110       120       130       
SRAAFP QPLGDRCIYYETTAMTWALAETNCMKLGGHLASIHSQEEHSFIQTLNAGVVWIGGSACLQ
       .:.  ::.   :. .::: :: ::. .::.::: ...:...::: ... ..::::::: .
gi|EU7 EPFDGRCFKLVTSRLTWAKAEKNCQAFGGNLASTRNSEDYNFIQQMTTELTWIGGSACQE
         220       250       280       310       340       370     

       140       150       160       170       180       190      
SRAAFP AGAWTWSDGTPMNFRSWCSTKPDDVLAACCMQMTAAADQCWDDLPCPASHKSVCAMTF*
       ..:: :::::::.   ::. .:: .:: ::.:....  .:::: ::     :::    :
gi|EU7 TNAWFWSDGTPMDKPLWCAGQPDGALAQCCLQINTGDGKCWDDQPCRNLLPSVCIKK**
         400       430       460       490       520       550    


>>ref|XM_028577120.1| PREDICTED: Perca fla                (1140 aa)
Frame: f initn: 400 init1: 183 opt: 518  Z-score: 627.1  bits: 125.4 E(55167125): 2.2e-25
Smith-Waterman score: 518; 42.2% identity (68.3% similar) in 180 aa overlap (24-196:33-566)

            30        40        50        60        70        80   
SRAAFP IVCTISTTRMLTVSLLVCAMMALTQANDDKILKGTATEAGPVSQRAPPNCPAGWQPLGDR
       :.: ::.:.::::::::::::::. :.:  . ....  ..  : .  : :::.:.  .::
ref|XM IICIISATKMLTVSLLVCAMMALATADDVDVASNNTDVTS--SYEEGPACPASWHKYNDR
               60        90       120       150         180        

            90       100       110       120             130       
SRAAFP CIYYETTAMTWALAETNCMKLGGHLASIHSQEEHSFIQ------TLNAGVVWIGGSACLQ
       :. :   .. :. :: ::..  :.:::.:: ::..:::      :    ..::::.:: .
ref|XM CFLYVPRTVDWSDAEKNCQSSKGNLASVHSIEEYQFIQMIITQQTHANPMTWIGGTACQK
      210       240       270       300       330       360        

       140       150       160        170       180       190      
SRAAFP AGAWTWSDGTPMNFRSWCSTKPDDVLAAC-CMQMTAAADQCWDDLPCPASHKSVCAMTF*
        . : :::: :..:  ::. .:... .   :..:. .  .::::. :  .  :::: . :
ref|XM HSNWFWSDGRPFSFTFWCAGEPNNAGGNQGCLRMNYGEHNCWDDIQCSDKLPSVCARNP*
      390       420       450       480       510       540        


>>gi|FJ826540.1| Perca flavescens type II a               (1218 aa)
Frame: f initn: 400 init1: 183 opt: 518  Z-score: 626.7  bits: 125.4 E(55167125): 2.3e-25
Smith-Waterman score: 518; 42.2% identity (68.3% similar) in 180 aa overlap (24-196:34-567)

            30        40        50        60        70        80   
SRAAFP IVCTISTTRMLTVSLLVCAMMALTQANDDKILKGTATEAGPVSQRAPPNCPAGWQPLGDR
       :.: ::.:.::::::::::::::. :.:  . ....  ..  : .  : :::.:.  .::
gi|FJ8 IICIISATKMLTVSLLVCAMMALATADDVDVASNNTDVTS--SYEEGPACPASWHKYNDR
        40        70       100       130         160       190     

            90       100       110       120             130       
SRAAFP CIYYETTAMTWALAETNCMKLGGHLASIHSQEEHSFIQ------TLNAGVVWIGGSACLQ
       :. :   .. :. :: ::..  :.:::.:: ::..:::      :    ..::::.:: .
gi|FJ8 CFLYVPRTVDWSDAEKNCQSSKGNLASVHSIEEYQFIQMIITQQTHANPMTWIGGTACQK
         220       250       280       310       340       370     

       140       150       160        170       180       190      
SRAAFP AGAWTWSDGTPMNFRSWCSTKPDDVLAAC-CMQMTAAADQCWDDLPCPASHKSVCAMTF*
        . : :::: :..:  ::. .:... .   :..:. .  .::::. :  .  :::: . :
gi|FJ8 HSNWFWSDGRPFSFTFWCAGEPNNAGGNQGCLRMNYGEHNCWDDIQCSDKLPSVCARNP*
         400       430       460       490       520       550     


>>gi|M96154.1|OSEAFP Osmerus mordax antifreeze            (611 aa)
Frame: f initn: 364 init1: 274 opt: 512  Z-score: 623.8  bits: 123.9 E(55167125): 3.4e-25
Smith-Waterman score: 512; 43.7% identity (71.3% similar) in 174 aa overlap (22-192:30-542)

              30        40        50        60        70        80 
SRAAFP IFIVCTISTTRMLTVSLLVCAMMALTQANDDKILKGTATEAGPVSQRAPPNCPAGWQPLG
       .. : .::::::: ..::::::.:::.: .    :  :. .:  : .   .::. :. ..
gi|M96 VINVKVISTTRML-AALLVCAMVALTRAANGDTGK-EAVMTGS-SGKNLTECPTDWKMFN
      30        60         90       120        150        180      

              90       100       110       120          130        
SRAAFP DRCIYYETTAMTWALAETNCMKLGGHLASIHSQEEHSFIQTLN-AGVV--WIGGSACLQA
        ::. ..   . :: :. .::: :..:::::: ::..:.. :. ::..  ::::: :  .
gi|M96 GRCFLFNPLQLHWAHAQISCMKDGANLASIHSLEEYAFVKELTTAGLIPAWIGGSDCHVS
        210       240       270       300       330       360      

      140       150       160       170       180       190  
SRAAFP GAWTWSDGTPMNFRSWCSTKPDDVLAACCMQMTAAADQCWDDLPCPASHKSVCA
         : : :.: :.: .::...:: .:. ::.:..... .::.: ::   : ::::
gi|M96 TYWFWMDSTSMDFTDWCAAQPDFTLTECCIQINVGVGKCWNDTPCTHLHASVCA
        390       420       450       480       510       540


>>gi|FJ826539.1| Perca flavescens type II a               (859 aa)
Frame: f initn: 404 init1: 178 opt: 502  Z-score: 609.9  bits: 121.8 E(55167125): 2e-24
Smith-Waterman score: 502; 41.5% identity (65.0% similar) in 183 aa overlap (24-196:33-581)

            30        40        50        60           70        80
SRAAFP IVCTISTTRMLTVSLLVCAMMALTQANDDKILKGTATEAGP---VSQRAPPNCPAGWQPL
       :.: ::::.::::::::::::::. :.:  . ....:  .     :    : :::.:   
gi|FJ8 IICIISTTKMLTVSLLVCAMMALATADDADVTSSNSTSNASNNSSSTTEGPACPASWTKY
               60        90       120       150       180       210

               90       100       110       120             130    
SRAAFP GDRCIYYETTAMTWALAETNCMKLGGHLASIHSQEEHSFIQ------TLNAGVVWIGGSA
       .:::. .   .. :. :: ::..  :.:::.:: ::..:::      :    ..::::. 
gi|FJ8 NDRCFLFVPRGLDWVDAEKNCQSSKGNLASVHSVEEYQFIQMIIKQQTHANPMTWIGGQD
              240       270       300       330       360       390

          140       150       160        170       180       190   
SRAAFP CLQAGAWTWSDGTPMNFRSWCSTKPDDVLAAC-CMQMTAAADQCWDDLPCPASHKSVCAM
        :. ..: :::: :. :  ::. .:..  .   :..:. .  .::::. :  .  :::: 
gi|FJ8 ALKNNVWFWSDGRPFYFTFWCAGEPNNGSGNQHCIEMNFGEHNCWDDVQCSIKLPSVCAR
              420       450       480       510       540       570

          
SRAAFP TF*
       . :
gi|FJ8 NP*
          


>>ref|XM_028577118.1| PREDICTED: Perca fla                (927 aa)
Frame: f initn: 404 init1: 178 opt: 502  Z-score: 609.4  bits: 121.8 E(55167125): 2.1e-24
Smith-Waterman score: 502; 41.5% identity (65.0% similar) in 183 aa overlap (24-196:121-669)

            30        40        50        60           70        80
SRAAFP IVCTISTTRMLTVSLLVCAMMALTQANDDKILKGTATEAGP---VSQRAPPNCPAGWQPL
       :.: ::::.::::::::::::::. :.:  . ....:  .     :    : :::.:   
ref|XM IICIISTTKMLTVSLLVCAMMALATADDADVTSSNSTSNASNNSSSTTEGPACPASWTKY
        130       160       190       220       250       280      

               90       100       110       120             130    
SRAAFP GDRCIYYETTAMTWALAETNCMKLGGHLASIHSQEEHSFIQ------TLNAGVVWIGGSA
       .:::. .   .. :. :: ::..  :.:::.:: ::..:::      :    ..::::. 
ref|XM NDRCFLFVPRGLDWVDAEKNCQSSKGNLASVHSVEEYQFIQMIIKQQTHANPMTWIGGQD
        310       340       370       400       430       460      

          140       150       160        170       180       190   
SRAAFP CLQAGAWTWSDGTPMNFRSWCSTKPDDVLAAC-CMQMTAAADQCWDDLPCPASHKSVCAM
        :. ..: :::: :. :  ::. .:..  .   :..:. .  .::::. :  .  :::: 
ref|XM ALKNNVWFWSDGRPFYFTFWCAGEPNNGSGNQHCIEMNFGEHNCWDDVQCSIKLPSVCAR
        490       520       550       580       610       640      

          
SRAAFP TF*
       . :
ref|XM NP*
          


>>ref|XM_028577117.1| PREDICTED: Perca fla                (834 aa)
Frame: f initn: 382 init1: 189 opt: 500  Z-score: 607.7  bits: 121.3 E(55167125): 2.7e-24
Smith-Waterman score: 500; 40.1% identity (64.7% similar) in 187 aa overlap (24-196:39-599)

            30        40        50        60               70      
SRAAFP IVCTISTTRMLTVSLLVCAMMALTQANDDKILKGTATE-------AGPVSQRAPPNCPAG
       :.: ::.:.::::::::::::::. :.:  . .....        ..  :    : :::.
ref|XM IICIISATKMLTVSLLVCAMMALATADDAYVTSSNSSSNVSNNSSSNSSSYAEGPACPAS
             60        90       120       150       180       210  

         80        90       100       110       120             130
SRAAFP WQPLGDRCIYYETTAMTWALAETNCMKLGGHLASIHSQEEHSFIQ------TLNAGVVWI
       :.  .:::. .   .. :. :: ::..: :.:::.:: ::..:::      :    ..::
ref|XM WHKYNDRCFLFIPRTLDWSEAEKNCQSLQGNLASVHSVEEYQFIQMIIKQQTHANPITWI
            240       270       300       330       360       390  

              140       150       160        170       180         
SRAAFP GGSACLQAGAWTWSDGTPMNFRSWCSTKPDDVLAAC-CMQMTAAADQCWDDLPCPASHKS
       ::    . ..: :::: :..:  ::. .:..  .   :.::. .  .::::. :  .  :
ref|XM GGHDSPKNNVWFWSDGRPFSFTFWCAGEPNNGYGNQDCIQMNFGEHNCWDDVQCSIKLPS
            420       450       480       510       540       570  

     190      
SRAAFP VCAMTF*
       ::: . :
ref|XM VCARNP*
              


>>gi|DQ062448.1| Osmerus mordax clone 14 typ              (539 aa)
Frame: f initn: 377 init1: 274 opt: 496  Z-score: 605.6  bits: 120.3 E(55167125): 3.5e-24
Smith-Waterman score: 496; 44.0% identity (70.5% similar) in 166 aa overlap (30-192:1-489)

      30        40        50        60        70        80         
SRAAFP TTRMLTVSLLVCAMMALTQANDDKILKGTATEAGPVSQRAPPNCPAGWQPLGDRCIYYET
       ::::: ..::::::.:::.: .    :  :. .:  : .   .::. :. .. ::  .. 
gi|DQ0 TTRML-AALLVCAMVALTRAANGDTGK-EAVMTGS-SGKNLTECPTDWKMFNGRCSLFNP
         10         40        70         100       130       160   

      90       100       110       120          130       140      
SRAAFP TAMTWALAETNCMKLGGHLASIHSQEEHSFIQTLN-AGVV--WIGGSACLQAGAWTWSDG
         . :: :. .::: :..:::::: ::..:.. :. ::..  ::::: :  .  : : :.
gi|DQ0 LQLHWAHAQISCMKDGANLASIHSLEEYAFVKELTTAGLIPAWIGGSDCHVSTYWFWMDS
           190       220       250       280       310       340   

        150       160       170       180       190  
SRAAFP TPMNFRSWCSTKPDDVLAACCMQMTAAADQCWDDLPCPASHKSVCA
       : :.: .::...:: .:. ::.:..... .::.: ::   : ::::
gi|DQ0 TSMDFTDWCAAQPDFTLTECCIQINVGVGKCWNDTPCTHLHASVCA
           370       400       430       460         


>>gi|FJ826541.1| Perca flavescens type II a               (869 aa)
Frame: f initn: 374 init1: 181 opt: 493  Z-score: 599.2  bits: 119.8 E(55167125): 8e-24
Smith-Waterman score: 493; 39.6% identity (64.7% similar) in 187 aa overlap (24-196:34-594)

            30        40        50        60               70      
SRAAFP IVCTISTTRMLTVSLLVCAMMALTQANDDKILKGTATE-------AGPVSQRAPPNCPAG
       :.: ::.:.::::::::::::::. :.:  . .....        ..  :    : :::.
gi|FJ8 IICIISATKMLTVSLLVCAMMALATADDAYVTSSNSSSNVSNNSSSNSSSYAEGPACPAS
        40        70       100       130       160       190       

         80        90       100       110       120             130
SRAAFP WQPLGDRCIYYETTAMTWALAETNCMKLGGHLASIHSQEEHSFIQ------TLNAGVVWI
       :.  .:::. .   .. :. :: ::..  :.:::.:: ::..:::      : .  ..::
gi|FJ8 WHKYNDRCFLFIPRTLDWSEAEKNCQSSKGNLASVHSVEEYQFIQMIIKQQTHGNPITWI
       220       250       280       310       340       370       

              140       150       160        170       180         
SRAAFP GGSACLQAGAWTWSDGTPMNFRSWCSTKPDDVLAAC-CMQMTAAADQCWDDLPCPASHKS
       ::    . ..: :::: :..:  ::. .:..  .   :.::. .  .::::. :  .  :
gi|FJ8 GGHDSPKNNVWFWSDGRPFSFTFWCAGEPNNGYGNQDCIQMNFGEHNCWDDVQCSIKLPS
       400       430       460       490       520       550       

     190      
SRAAFP VCAMTF*
       ::: . :
gi|FJ8 VCARNP*
       580    


>>ref|XM_018680358.1| PREDICTED: Lates cal                (912 aa)
Frame: f initn: 281 init1:  93 opt: 483  Z-score: 587.1  bits: 117.7 E(55167125): 3.8e-23
Smith-Waterman score: 483; 39.9% identity (65.6% similar) in 183 aa overlap (21-192:18-566)

                30        40        50           60        70      
SRAAFP IIFIVC-TISTTRMLTVSLLVCAMMALTQAN---DDKILKGTATEAGPVSQRAPPNCPAG
       :  ..: .. :..:::: .:::::::::.:    ..:  :   .    . .:.  .: .:
ref|XM IYELLCLSFPTVKMLTVWVLVCAMMALTRAAALPEEKAKKDDQAVETDLVKRTYYGCSSG
          30        60        90       120       150       180     

         80        90       100       110       120             130
SRAAFP WQPLGDRCIYYETTAMTWALAETNCMKLGGHLASIHSQEEHSFIQTL------NAGVVWI
       :. .. ::...    :::: :: :: ..::.:::.::..:.  :: :      .  ..:.
ref|XM WSRFNRRCFHFVPKPMTWAQAERNCRSMGGNLASVHSMQEYHEIQRLIMTATYEYKAAWL
         210       240       270       300       330       360     

              140       150       160        170       180         
SRAAFP GGSACLQAGAWTWSDGTPMNFRSWCSTKPDDVL-AACCMQMTAAADQCWDDLPCPASHKS
       :::   . ..: :::: :... .::  .:..      ::::. ::..::::: : :   :
ref|XM GGSDAQEENVWLWSDGRPFHYSNWCHGEPNNYRRQQNCMQMNHAAQKCWDDLQCNAHLPS
         390       420       450       480       510       540     

     190  
SRAAFP VCA
       .::
ref|XM ICA
          


>>ref|XM_018703530.1| PREDICTED: Lates cal                (652 aa)
Frame: f initn: 281 init1:  93 opt: 479  Z-score: 584.4  bits: 116.7 E(55167125): 5.3e-23
Smith-Waterman score: 479; 41.0% identity (66.1% similar) in 183 aa overlap (21-192:3-548)

                30        40           50        60        70      
SRAAFP IIFIVC-TISTTRMLTVSLLVCAMMALTQA---NDDKILKGTATEAGPVSQRAPPNCPAG
       :  ..: .. :..:::: .:::::::::.:    ..:  :   .:.  : .:.  .: .:
ref|XM IYELLCLSFPTVKMLTVWVLVCAMMALTRAVALPEEKAKKDDQAETDLV-KRTYYGCSSG
               30        60        90       120        150         

         80        90       100       110       120             130
SRAAFP WQPLGDRCIYYETTAMTWALAETNCMKLGGHLASIHSQEEHSFIQTL------NAGVVWI
       :. .. ::...    :::: :: :: ..::.:::.:: .:.  :: :      .  ..:.
ref|XM WSRFNRRCFHFVPKPMTWAQAERNCRSMGGNLASVHSVQEYHEIQRLIMTATYEYKAAWL
     180       210       240       270       300       330         

              140       150       160        170       180         
SRAAFP GGSACLQAGAWTWSDGTPMNFRSWCSTKPDDVL-AACCMQMTAAADQCWDDLPCPASHKS
       :::   . ..: :::: :... .::  .:..      ::::. ::..::::: : :   :
ref|XM GGSDAQEENVWLWSDGRPFHYSNWCHGEPNNYRRQQNCMQMNHAAQKCWDDLQCNAHLPS
     360       390       420       450       480       510         

     190  
SRAAFP VCA
       .::
ref|XM ICA
     540  


>>ref|XM_018703522.1| PREDICTED: Lates cal                (897 aa)
Frame: f initn: 281 init1:  93 opt: 479  Z-score: 582.4  bits: 116.8 E(55167125): 6.8e-23
Smith-Waterman score: 479; 39.9% identity (65.0% similar) in 183 aa overlap (21-192:3-551)

                30        40           50        60        70      
SRAAFP IIFIVC-TISTTRMLTVSLLVCAMMALTQA---NDDKILKGTATEAGPVSQRAPPNCPAG
       :  ..: .. :..:::: .:::::::::.:    ..:  :   .    . .:.  .: .:
ref|XM IYELLCLSFPTVKMLTVWVLVCAMMALTRAVALPEEKAKKDDQAVETDLVKRTYYGCSSG
               30        60        90       120       150       180

         80        90       100       110       120             130
SRAAFP WQPLGDRCIYYETTAMTWALAETNCMKLGGHLASIHSQEEHSFIQTL------NAGVVWI
       :. .. ::...    :::: :: :: ..::.:::.:: .:.  :: :      .  ..:.
ref|XM WSRFNRRCFHFVPKPMTWAQAERNCRSMGGNLASVHSVQEYHEIQRLIMTATYEYKAAWL
              210       240       270       300       330       360

              140       150       160        170       180         
SRAAFP GGSACLQAGAWTWSDGTPMNFRSWCSTKPDDVL-AACCMQMTAAADQCWDDLPCPASHKS
       :::   . ..: :::: :... .::  .:..      ::::. ::..::::: : :   :
ref|XM GGSDAQEENVWLWSDGRPFHYSNWCHGEPNNYRRQQNCMQMNHAAQKCWDDLQCNAHLPS
              390       420       450       480       510       540

     190  
SRAAFP VCA
       .::
ref|XM ICA
          


>>ref|XM_028564253.1| PREDICTED: Perca fla                (708 aa)
Frame: f initn: 394 init1: 197 opt: 476  Z-score: 580.3  bits: 116.0 E(55167125): 9e-23
Smith-Waterman score: 476; 44.3% identity (62.1% similar) in 174 aa overlap (26-192:1-486)

          30        40         50        60        70        80    
SRAAFP CTISTTRMLTVSLLVCAMMALTQA-NDDKILKGTATEAGPVSQRAPPNCPAGWQPLGDRC
       : ::: ..: ::::::::::::.: . ..:.: ...            ::.::   . ::
ref|XM CIISTMKVLIVSLLVCAMMALTRAAGKSHIVKRSSS------------CPSGWTGYNGRC
         10        40        70       100                   130    

           90       100       110       120             130        
SRAAFP IYYETTAMTWALAETNCMKLGGHLASIHSQEEHSFIQTLNAGVV------WIGGSACLQA
       . :  :::::: :: ::.  ::.:::.:: :::  ::..   ..      :.:::   : 
ref|XM FLYVPTAMTWADAEKNCLYHGGNLASVHSFEEHHVIQSMILRITHMYPLTWLGGSDAQQE
          160       190       220       250       280       310    

      140       150       160       170       180       190  
SRAAFP GAWTWSDGTPMNFRSWCSTKPDDVLAACCMQMTAAADQCWDDLPCPASHKSVCA
       :.: ::::::. :  :   .::.   : :. :. . .. .:: ::     ::::
ref|XM GSWFWSDGTPFRFNFWSPGQPDNRGNAHCLLMNFGDQNKFDDQPCSFRMPSVCA
          340       370       400       430       460        


>>ref|XM_003457801.5| PREDICTED: Oreochrom                (1273 aa)
Frame: f initn: 203 init1: 203 opt: 479  Z-score: 580.3  bits: 116.9 E(55167125): 9e-23
Smith-Waterman score: 479; 38.1% identity (65.6% similar) in 189 aa overlap (14-196:232-792)

            20        30        40        50          60        70 
SRAAFP ISTAGLSIIFIVCTISTTRMLTVSLLVCAMMALTQAND--DKILKGTATEAGPVSQRAPP
       ..:. :...  . .: : . ::.: . ::..:::.: .  :   .. :   . . .::  
ref|XM LQTVILGLVSQILNI-TMKKLTASAIFCAIIALTMAAEPRDGAEESLAPAKSHIVKRA-T
           250        280       310       340       370       400  

              80        90       100       110       120           
SRAAFP NCPAGWQPLGDRCIYYETTAMTWALAETNCMKLGGHLASIHSQEEHSFIQTLNAGV----
       .:: ::  .. ::. :  ..:.:: :: ::. .:.::::.::  :.  :: :.:      
ref|XM DCPDGWTLISGRCFRYVPAVMNWANAEINCLYMGAHLASVHSWWEYHQIQRLTAPYGYRE
             430       460       490       520       550       580 

       130       140       150       160       170       180       
SRAAFP VWIGGSACLQAGAWTWSDGTPMNFRSWCSTKPDDVLAACCMQMTAAADQCWDDLPCPASH
       .:.::.     :.: ::::. ...: ::: .:....   :.::.  . .::::: :   .
ref|XM AWLGGTDAAYEGVWFWSDGSRFDYRRWCSGEPNNMFFQHCLQMNYRGPKCWDDLWCSYHR
             610       640       670       700       730       760 

       190      
SRAAFP KSVCAMTF*
        ::::: .:
ref|XM PSVCAMEI*
             790


>>ref|XM_030429850.1| PREDICTED: Sparus au                (661 aa)
Frame: f initn: 410 init1: 273 opt: 475  Z-score: 579.6  bits: 115.8 E(55167125): 9.9e-23
Smith-Waterman score: 475; 41.4% identity (65.5% similar) in 174 aa overlap (29-196:22-543)

       30        40        50        60        70        80        
SRAAFP STTRMLTVSLLVCAMMALTQANDDKILKGTATEAGPVSQRAPPNCPAGWQPLGDRCIYYE
       :.:.:::::::::::::::.: . .   ... :.     .  :.:: ::   . ::. : 
ref|XM SNTKMLTVSLLVCAMMALTRAAEGETDLNSGPEVTSSIAELMPSCPPGWTSYSIRCFLYV
            40        70       100       130       160       190   

       90       100       110       120             130       140  
SRAAFP TTAMTWALAETNCMKLGGHLASIHSQEEHSFIQTL----NAG--VVWIGGSACLQAGAWT
        ..:::: ::  :.. ::.:::.:: .:.  :::.    ..:  ..:.::    : :.: 
ref|XM PSTMTWANAEKYCQSQGGNLASVHSFDEQHVIQTMIQRQTSGYPLAWLGGCDAAQEGTWF
           220       250       280       310       340       370   

            150       160       170       180       190      
SRAAFP WSDGTPMNFRSWCSTKPDDVLAACCMQMTAAADQCWDDLPCPASHKSVCAMTF*
       ::::::...  : . .::.   : :. :. . .. .:: ::   .  :::  .:
ref|XM WSDGTPFSYNYWATGQPDNRANANCLLMNFGDEKKFDDQPCNYIKPFVCAKKL*
           400       430       460       490       520       


>>ref|XM_025901702.1| PREDICTED: Oreochrom                (1726 aa)
Frame: f initn: 249 init1: 203 opt: 479  Z-score: 578.4  bits: 117.0 E(55167125): 1.1e-22
Smith-Waterman score: 479; 38.1% identity (65.6% similar) in 189 aa overlap (14-196:685-1245)

            20        30        40        50          60        70 
SRAAFP ISTAGLSIIFIVCTISTTRMLTVSLLVCAMMALTQAND--DKILKGTATEAGPVSQRAPP
       ..:. :...  . .: : . ::.: . ::..:::.: .  :   .. :   . . .::  
ref|XM LQTVILGLVSQILNI-TMKKLTASAIFCAIIALTMAAEPRDGAEESLAPAKSHIVKRA-T
          700        730       760       790       820       850   

              80        90       100       110       120           
SRAAFP NCPAGWQPLGDRCIYYETTAMTWALAETNCMKLGGHLASIHSQEEHSFIQTLNAGV----
       .:: ::  .. ::. :  ..:.:: :: ::. .:.::::.::  :.  :: :.:      
ref|XM DCPDGWTLISGRCFRYVPAVMNWANAEINCLYMGAHLASVHSWWEYHQIQRLTAPYGYRE
            880       910       940       970      1000      1030  

       130       140       150       160       170       180       
SRAAFP VWIGGSACLQAGAWTWSDGTPMNFRSWCSTKPDDVLAACCMQMTAAADQCWDDLPCPASH
       .:.::.     :.: ::::. ...: ::: .:....   :.::.  . .::::: :   .
ref|XM AWLGGTDAAYEGVWFWSDGSRFDYRRWCSGEPNNMFFQHCLQMNYRGPKCWDDLWCSYHR
           1060      1090      1120      1150      1180      1210  

       190      
SRAAFP KSVCAMTF*
        ::::: .:
ref|XM PSVCAMEI*
           1240 


Frame: f initn: 127 init1:  82 opt: 342  Z-score: 416.5  bits: 87.0 E(55167125): 1.2e-13
Smith-Waterman score: 342; 37.3% identity (62.7% similar) in 142 aa overlap (32-172:170-586)

              40        50        60        70        80        90 
SRAAFP RMLTVSLLVCAMMALTQANDDKILKGTATEAGPVSQRAPPNCPAGWQPLGDRCIYYETTA
       ..::.  : :::::.: :. .. : .      : .    : :: ::  .:.::. :. . 
ref|XM KLLTLCALFCAMMAVTTAGGESHLFNWCIYC-P-DGWNQPRCPYGWTQVGSRCFIYNQNP
     170       200       230       260         290       320       

             100       110        120       130       140       150
SRAAFP MTWALAETNCMKLGGHLASIHSQEEHS-FIQTLNAGVVWIGGSACLQAGAWTWSDGTPMN
       :.:  :. .: .::..:::.:.  ::. .:: ..   .:::::     : : :.::: ..
ref|XM MSWDSAKRHCWELGANLASVHTYWEHQRIIQLFGNWPAWIGGSKG-PKGNWWWNDGTSFS
       350       380       410       440       470        500      

              160       170  
SRAAFP FRSWCSTKPDDVLAACCMQMTA
       :  ::  .:..     :.::..
ref|XM FS*WCWGEPSNGYYENCLQMSS
        530       560        


>>ref|XM_018680359.1| PREDICTED: Lates cal                (856 aa)
Frame: f initn: 281 init1:  93 opt: 474  Z-score: 576.8  bits: 115.7 E(55167125): 1.4e-22
Smith-Waterman score: 474; 42.1% identity (66.7% similar) in 171 aa overlap (32-192:1-510)

              40        50           60        70        80        
SRAAFP RMLTVSLLVCAMMALTQAN---DDKILKGTATEAGPVSQRAPPNCPAGWQPLGDRCIYYE
       .:::: .:::::::::.:    ..:  :   .:.  : .:.  .: .::. .. ::... 
ref|XM KMLTVWVLVCAMMALTRAAALPEEKAKKDDQAETDLV-KRTYYGCSSGWSRFNRRCFHFV
         10        40        70       100        130       160     

       90       100       110       120             130       140  
SRAAFP TTAMTWALAETNCMKLGGHLASIHSQEEHSFIQTL------NAGVVWIGGSACLQAGAWT
          :::: :: :: ..::.:::.::..:.  :: :      .  ..:.:::   . ..: 
ref|XM PKPMTWAQAERNCRSMGGNLASVHSMQEYHEIQRLIMTATYEYKAAWLGGSDAQEENVWL
         190       220       250       280       310       340     

            150       160        170       180       190  
SRAAFP WSDGTPMNFRSWCSTKPDDVL-AACCMQMTAAADQCWDDLPCPASHKSVCA
       :::: :... .::  .:..      ::::. ::..::::: : :   :.::
ref|XM WSDGRPFHYSNWCHGEPNNYRRQQNCMQMNHAAQKCWDDLQCNAHLPSICA
         370       400       430       460       490      


>>ref|XM_030754005.1| PREDICTED: Archocent                (702 aa)
Frame: f initn: 301 init1: 222 opt: 471  Z-score: 574.5  bits: 114.9 E(55167125): 1.9e-22
Smith-Waterman score: 471; 41.4% identity (66.9% similar) in 169 aa overlap (30-193:43-534)

      30        40        50        60         70        80        
SRAAFP TTRMLTVSLLVCAMMALTQANDDKILKGTATEAGPVSQ-RAPPNCPAGWQPLGDRCIYYE
       : ..:.::  .::.:::: :     ..: .   :::.  ..  .:: ::  ...::..: 
ref|XM TMKLLSVSAHLCALMALTFA-----VEGDSDAEGPVTTVKSHSGCPRGWIEFNNRCFHYV
               70       100            130       160       190     

       90       100       110       120           130       140    
SRAAFP TTAMTWALAETNCMKLGGHLASIHSQEEHSFIQTLNA--GV--VWIGGSACLQAGAWTWS
       :: :::: :: ::. :::.:::.:.. :.  :: :.:  :   .::::.  .:  .: ::
ref|XM TTRMTWANAEKNCLLLGGNLASVHNDMEYFEIQKLTATHGYRQAWIGGTDAVQKKVWFWS
         220       250       280       310       340       370     

          150       160       170       180       190   
SRAAFP DGTPMNFRSWCSTKPDDVLAACCMQMTAAADQCWDDLPCPASHKSVCAM
       ::::... .::  . ..     :..:. .. .::::. :     ::::.
ref|XM DGTPFHYSNWCPGESSNGRNDHCLRMNYSGAKCWDDVRCAIRLPSVCAI
         400       430       460       490       520    


196 residues in 1 query   sequences
242370672807 residues in 54289499 library sequences
 Tcomplib [36.3.8h Aug, 2019] (32 proc in memory [15G])
 start: Mon Apr 20 14:58:45 2020 done: Mon Apr 20 15:27:46 2020
 Total Scan time: 12525.890 Total Display time:  0.870

Function used was TFASTX [36.3.8h Aug, 2019]