/* sam.h -- format-neutral SAM/BAM API. Copyright (C) 2009, 2013-2015 Genome Research Ltd. Author: Heng Li Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions: The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software. THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE. */ #ifndef BAM_SAM_H #define BAM_SAM_H #include "htslib/sam.h" #include "bam.h" /*! @header This file provides higher level of I/O routines and unifies the APIs for SAM and BAM formats. These APIs are more convenient and recommended. @copyright Genome Research Ltd. */ /*! @typedef @abstract SAM/BAM file handler @field type type of the handler; bit 1 for BAM, 2 for reading and bit 3-4 for flag format @field bam BAM file handler; valid if (type&1) == 1 @field tamr SAM file handler for reading; valid if type == 2 @field tamw SAM file handler for writing; valid if type == 0 @field header header struct */ typedef struct { samFile *file; struct { BGZF *bam; } x; // Hack so that fp->x.bam still works bam_hdr_t *header; unsigned short is_write:1; } samfile_t; #ifdef __cplusplus extern "C" { #endif /*! @abstract Open a SAM/BAM file @param fn SAM/BAM file name; "-" is recognized as stdin (for reading) or stdout (for writing). @param mode open mode /[rw](b?)(u?)(h?)([xX]?)/: 'r' for reading, 'w' for writing, 'b' for BAM I/O, 'u' for uncompressed BAM output, 'h' for outputing header in SAM, 'x' for HEX flag and 'X' for string flag. If 'b' present, it must immediately follow 'r' or 'w'. Valid modes are "r", "w", "wh", "wx", "whx", "wX", "whX", "rb", "wb" and "wbu" exclusively. @param aux auxiliary data; if mode[0]=='w', aux points to bam_header_t; if strcmp(mode, "rb")!=0 and @SQ header lines in SAM are absent, aux points the file name of the list of the reference; aux is not used otherwise. If @SQ header lines are present in SAM, aux is not used, either. @return SAM/BAM file handler */ samfile_t *samopen(const char *fn, const char *mode, const void *aux); /*! @abstract Close a SAM/BAM handler @param fp file handler to be closed */ void samclose(samfile_t *fp); /*! @abstract Read one alignment @param fp file handler @param b alignment @return bytes read */ static inline int samread(samfile_t *fp, bam1_t *b) { return sam_read1(fp->file, fp->header, b); } /*! @abstract Write one alignment @param fp file handler @param b alignment @return bytes written */ static inline int samwrite(samfile_t *fp, const bam1_t *b) { return sam_write1(fp->file, fp->header, b); } /*! @abstract Load BAM/CRAM index for use with samfetch() @param fp file handler @param fn name of the BAM or CRAM file (NOT the index file) @return pointer to the index structure */ static inline bam_index_t *samtools_sam_index_load(samfile_t *fp, const char *fn) { return sam_index_load(fp->file, fn); } #undef sam_index_load #define sam_index_load(fp,fn) (samtools_sam_index_load((fp), (fn))) /*! @abstract Retrieve the alignments overlapping the specified region. @discussion A user defined function will be called for each retrieved alignment ordered by its start position. @param fp file handler @param idx index returned by sam_index_load() @param tid chromosome ID as is defined in the header @param beg start coordinate, 0-based @param end end coordinate, 0-based @param data user provided data (will be transferred to func) @param func user defined function */ int samfetch(samfile_t *fp, const bam_index_t *idx, int tid, int beg, int end, void *data, bam_fetch_f func); /*! @abstract Get the pileup for a whole alignment file @param fp file handler @param mask mask transferred to bam_plbuf_set_mask() @param func user defined function called in the pileup process #param data user provided data for func() */ int sampileup(samfile_t *fp, int mask, bam_pileup_f func, void *data); char *samfaipath(const char *fn_ref); int samthreads(samfile_t *fp, int n_threads, int n_sub_blks); #ifdef __cplusplus } #endif #endif