updated Jan. 24, 2023
| Scheduled lab sessions |
| Week |
Agro I |
Regeneration |
Genomic DNA |
Agro II |
|
| Dec. 5 | make media for Agro get Agro cells from Sonia streak out and isolate colonies on Kanamycin, and non-Kan control plates |
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| Dec. 12 |
inoculate liquid cultures from colonies split into aliquots alkaline plasmid prep of pI121 from Agro |
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| Dec. 19 |
plant tobacco RE digests of pI121 to confirm identity of plasmid |
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| Dec. 25 |
Disha leaves |
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| Jan. 9 |
make MS plates for transformation sterilize materials |
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| Jan. 16 |
Disha returns |
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| Transform A.t. with pBI121; verify KanR do plasmid prep to verify DNA make glycerol stocks for -80 Prepare materials and media for Agro transformation |
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| Feb. 6,7 |
inoculate leaf discs
with Agro |
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| prepare media and supplies |
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| Feb. 13,14 |
Plant Regeneration Lab
session |
plant transgenic tobacco and non-transgenic
controls |
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| Feb. 27 |
TAs run through CTAB DNA extraction nanopore quantification RE digest, verify on gel PCR, verify on gel |
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| March 6 |
TAs run through GUS assay |
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| March 13 |
CTAB DNA extraction nanodrop quantification of DNA |
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| March 20 |
RE digest start PCR Run gel of RE DNA |
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| March 27 |
run gel of PCR products | ||||
| April 3 |
final photos of transformants |
GUS assay of leaves | |||
| April 11 |
final photos of regenerating plants |
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| April 12 |
report due |
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| April 25 |
report due |
report due |