IN-CLASS EXERCISE: Semi-Conservative Replication 5-bromo-deoxyuridine
(BUdr, BrdU) can incorporate into DNA in place of Thymidine. BuDR
can be detected by red flourescence when irradiated with blue
light. Cells are grown in culture for one generation with BrdU,
and then transferred to fresh culture without dye. They then are
allowed to go for one more cell division. The protocol is
For each set of chromosomal DNAs indicate how BUdr is distributed.
Indicate what happens to label if sister chromatid exchange
(crossing over between newly-replicated chromosomes) occurs.