IN-CLASS EXERCISE: Semi-Conservative Replication
5-bromo-deoxyuridine (BUdr, BrdU) can incorporate into DNA in place of Thymidine. BuDR can be detected by red flourescence when irradiated with blue light. Cells are grown in culture for one generation with BrdU, and then transferred to fresh culture without dye. They then are allowed to go for one more cell division. The protocol is illustrated below:


For each set of chromosomal DNAs indicate how BUdr is distributed. Indicate what happens to label if sister chromatid exchange (crossing over between newly-replicated chromosomes) occurs.


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