last  page PLNT3140 Introductory Cytogenetics
Lecture 17, part 1 of 4
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November 9, 2017
BUILDING GENOMIC MAPS

"Taking ownership of the genome."


Learning checklist:

1. Be familiar with the goals of genomics projects:  a fully-integrated map containing genetic data, overlapping clones, DNA sequence, and other annotation.

2. Know what genomic libraries are, and know how to calculate the number of randomly-chosen clones needed to ensure complete coverage in a library.

3. Know what BACs are, and their advantages and disadvantages.

4. Understand the principles behind map-based cloning and how to construct contigs from overlapping clones.

5. Know the basic principles behind RNAseq analysis of gene expression.


II. THE GOAL: A FULLY-INTEGRATED MAPS CONTAINING GENETIC DATA, OVERLAPPING CLONES, DNA SEQUENCE, AND OTHER INFORMATION.


Let's start by giving an example of what it is most genome programs hope to accomplish. Conceptually, this can be broken down into successive steps, each at a finer level of resolution:

The result is an integrated model of the genome. The entire genome of the nematode worm, Caenorabiditis elegans has been completeley mapped and sequenced. These data are taken from ACEDB (A C. elegans Database) at http://www.sanger.ac.uk
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last  page PLNT3140 Introductory Cytogenetics
Lecture 17, part 1 of 4
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