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Lecture 17, part 3 of 4
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III.GENOMIC LIBRARIES (cond.)

C. MAP-BASED CLONING (Positional cloning)

Given a molecular marker, clone the associated gene.

D. Constructing contigs by hybridization.

An ordered library is a library of overlapping clones, such that each sequence outside of overlap regions is represented exactly once. Put another way, think of the chromosome as being split into short fragments laid end to end. Contigs are assembled by successive hybridization, fingerprinting, or sequencing experiments. Since thousands of clones have to be compared against each other to detect overlaps, computers are used to do pairwise comparisons.


(Figure from http://www.gdb.org/Dan/fig9.html)
 

Once you have an ordered library:


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