Lab Protocols

Notebooks and recordkeeping

• Writing experimental protocols

Bacterial manipulation

• E. coli transformation: rapid protocol
• Agro transformation: liquid nitrogen protocol

DNA manipulation

• Plasmid preparation: PEG protocol
• Genomic DNA extraction: CTAB protocol
• Genomic DNA extraction: simplified CTAB protocol for PCR
• Sticky DNA end polishing: pfu protocol

RNA manipulation

• Total RNA extraction: VRC protocol
• mRNA preparation: hot TE protocol

Transgenic plants

• Finding lines homozygous for a transgene

Gene Arrays

• Storage of arrayed clones
• DIG-labeled probes