Screen for fungal-induced genes
Clones that hybridize more strongly with cDNA probe
from L. maculans-inoculated leaves, than with cDNA from uninoculated leaves,
represent fungal-induced transcripts.
Choose for further study:
-
fungal-induced genes
- likely to be involved in defense responses
-
non-induced genes - needed
for comparison with induced genes
-
weakly-expressed genes
- likely to be previously "unknown" genes