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• Contact
• Instrument
• Cells Available
• Useful Links
• Home
• Department

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Joe O'Neil
Department of Chemistry
Room 520 H Parker Building
University of Manitoba
144 Dysart Rd.
Winnipeg, Manitoba, Canada, R3T 2N2

Tel (204) 256-0361
Fax (204) 474-7608

E-mail: Joe.ONeil@umanitoba.ca

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Instrument

Circular dichroism is most commonly used in biochemistry to measure protein secondary structure but it can also probe protein tertiary structure and nucleic acid structure. Our NSERC-funded Jasco J810 Circular Dishroism Spectropolarimeter-Fluorometer can measure circular dichroism, absorption, and fluorescence from 170 nm to 1100 nm. It is equipped with a Julabo circulating water bath and a Peltier thermostatted cell holder with stirrer for temperature control. The instrument allows concurrent measurements of all 3 phenomena so, for example, it is possible to measure far UV CD and fluorescence during thermal melting of a protein. For more information see the brochure and specification sheet. To book an appointment to use the instrument contact J. O'Neil above.

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Cells Available

Water-Jacketed Cylindrical Quartz Cells

•  50 mm pathlength, 4.0 mL
  
•  10 mm pathlength, 790 μL
  
•    5 mm pathlength, 605 μL
  
•    1 mm pathlength, 175 μL
  
• 0.5 mm pathlength,125 μL
  
• 0.1 mm pathlength, 85 μL
  

Cylindrical Quartz Cells

• 50 mm pathlength, 14.2 mL
  
• 10 mm pathlength, 2.84 mL
  

Rectangular Quartz Cell

• 10 mm pathlength, 3.5 mL
  

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Useful Links

Dichroweb Server

CD Animation

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http://home.cc.umanitoba.ca/~joneil/

Maintained by J. O'Neil

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