CHEM 4370

Glycobiology and Protein Activation

CHEM 4370 Glycobiology and Protein Activation (3) The role of carbohydrate containing biomolecules in biochemistry and their importance for understanding some genetic diseases. The importance of limited proteolysis in activation of biomolecules. Prerequisite: CHEM 2370 (MBIO 2370) (or the former 002.235 (060.235)) (C).

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Course Outline - 2009

General Description of the Course:

This course deals with the structure and biosynthesis of macromolecules within cells. The emphasis is on mammalian cells. The primary focus is the synthesis and function of oligosaccharide containing molecules and the role of these in associated diseases. Techniques for the analysis of these structures will also be discussed. The course may also cover activation of proteins by limited proteolysis, if time permits.

Lectures:

Instructors:

Recommended Texts (One of the following):

  1. "Essentials of Glycobiology" Varki et al. (eds);
    1999 edition; 3 copies; QP 552 G59 E78.
    2008 edition; 1 copy; QP552 G59 E87
  2. "Introduction to Glycobiology by Taylor and Drickamer
    2003 edition; 1 copy; QP702 S85 T39
    2006 edition; 1 copy; QP702 G577 T39
  3. "Functional and Molecular Glycobiology" by Brooks, Dwek and Schumacher
    2002 edition; 1 copy; QP 702 G577 B76

Regarding the text books:
Copies of the 3 textbooks are available through the bookstore.
As indicated above, copies of the 3 textbooks are on 2h-reserve through the Science and Technology Library:

The Varki text is an in-depth book which will cover all topics within the course.
The Taylor book is less detailed and does not cover all topics but is easy to follow.
The Brooks text covers most topics in good detail.

All relevant material will be covered in the lectures, however, the texts are recommended for averall understanding of the course.

Allocation of Marks:


Final Exam Review

Students in the Faculty of Science are permitted to review their final exams before the deadline for appealing final grades (Final Grade Appeal). If you wish to view your final exam please go to the Department of Chemistry general office (360 Parker Building), fill in an application form, and pay the $5.00 fee.

Note:

Project :

Projects consist of essays of about 2000 words complete with diagrams as appropriate.
Project topics will be assigned by mid January.
Projects must be handed in by Friday March 6, 2009 (5 PM) to Room 520B Parker.

Note:

Death in the immediate family, and serious illness are the only valid reasons for missing exams. A medical certificate must be provided as proof of illness. Late penalty for papers is 10% per day.

Academic Dishonesty: Please visit the Faculty of Science web site Cheating, Plagiarism etc.

Course Outline:

Saccharide structure and nomenclature:

  1. Monosaccharides, oligosaccharides, polysaccharides, glycoconjugates, glycans, glycoconjugates, glycosidic linkage

    2. N-glycans:

  2. Mode of attachment to protein, consensus sequence
  3. Structure: high Man, complex, hybrid, variations
  4. General functions
  5. Biosynthesis
  6. Significance of sialylation, fucosylation and polylactosamine chains
  7. Enzymes: Flippase, OST-complex, glycosyltransferases and glycosidases
  8. Substrates: nucleotide sugars, dolichol P-sugars, oligosaccharide substrates
  9. Intra-Golgi transport
  10. Congenital Disorders of Glycosylation (CDG's): autosomal recessive diseases of glycosylation, Types I and II, treatment of diseases
  11. Animal Lectins involved with quality control and intracellular transport (Calnexin/Calreticulin and P-lectins)
  12. Lysosomal enzymes: genetic defects in glycoconjugate degradation, Man-6-P receptors, synthesis of Man-6-P, P-lectins, lysosomal trafficking
  13. Plant Lectins and Oligosaccharide synthesis inhibitors: tunicamycin, plant alkaloids, importance of glycosylation, neuraminidase inhibitors, synthetic inhibitors, carbohydrate mutants
  14. Role of dolichol-P, glycosyltransferases and glucosidases

    O-glycans

  15. Structure and biosynthesis transferases involved , example glycoproteins , mucins

    Glycolipids:

  16. Structure and biosynthesis: glycosphingolipids, gangliosides and globosides

    Animal Lectins:

  17. Domain structure, lectin-ligand interactions
  18. Subtypes: Calnexin/Calreticulin, L-, P-, C-, I- and S-lectins

    GPI-anchors:

  19. Structure, biosynthesis and biological significance

    Glycosaminoglycans and Proteoglycans:

  20. Structure, biosynthesis, and biological significance

    Structural analysis of carbohydrates:

  21. General carbohydrate detection, gel filtration, CE, HPLC, NMR, mass spec, IEF, glycomics, glycan arrays

    Clinical applications (Time permitting):

  22. ABO blood group antigens
  23. Cancer and metastasis
  24. Prion disease, and the role of glycosylation
  25. Glycation end products
  26. Additional topics

Useful Web Sites for CHEM 4370:

Carbohydrates

Glycobiology Resources

Glycobiology Research and Training Center

Entrez

Return to the Chemistry Department Course Descriptions

http://home.cc.umanitoba.ca/~joneil/CHEM4370.Course.outline.htm

Maintained by J. O'Neil

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