CHEM 4700
Advanced Biochemistry Laboratory
CHEM 4700 Advanced Biochemistry Laboratory (3)L (Formerly 002.470) (Lab required) A laboratory and workshop consisting of lectures, problem solving, and advanced instrumental techniques such as magnetic resonance spectroscopy, mass spectrometry, circular dichroism, x-ray crystallography, fluorescence spectroscopy and computer analysis of protein sequences. This course is required for all final year Honours students in Biochemistry. Prerequisite or concurrent requirement: CHEM 4620 or CHEM 4621; and CHEM 4630 or CHEM 4631.
Announcements
Please read this important notice from the Faculty of Science: Registration Advisory.
Note also that Wednesday September 21 is the last day for adding courses and VW day is Friday March 16.
Course Outline 2011-12
Times:
- Tuesdays 2:30-5:30
- Please note that there is no laboratory during the first week of classes.
Locations:
See the schedule below:
Instructors:
| Dr. Mazdak Khajehpour Room 468 Parker Phone: 272-1546 E-mail:khajehpo@cc.umanitoba.ca |
Dr. Brian Mark Room 420 Buller Building Phone: 480-1430 E-mail: brian_mark@umanitoba.ca |
| Dr. Sean McKenna Room 380 Parker Building Phone: 272-1562 E-mail: mckenna@cc.umanitoba.ca |
Dr. Joe O'Neil Room 390 Parker Building Phone: 474-6697 E-mail: joneil@cc.umanitoba.ca |
| Dr. Hélène Perreault Room 550 Parker Phone: 474-7418 E-mail:perreau@cc.umanitoba.ca |
Dr. Jörg Stetefeld Room 426 Parker Phone: 474-9731 E-mail:stetefel@cc.umanitoba.ca |
Recommended References:
- Principles of Physical Biochemistry 2nd Ed. by K. van Holde, W. C. Johnson, P S. Ho 2006, Pearson / Prentice Hall QP517.P49V36.
Biophysical Chemistry Part 2 R. Cantor & P. R. Schimmel Freeman & Co. QH345.C36
- Instrumental Methods of Analysis
H. Willard et al. 1988
QD79.I5I52
- In my office is a collection of articles which provide some of the theory behind the experiments we will be carrying out. You are welcome to borrow this from me.
- Copies of previous years' examinations are available on-line at:
Online Examinations, and below.
Working through them will help prepare you for the final examination.
Evaluation:
| Lab reports and problem sets 5% each: | 45% |
| Research Proposal: | 20% | Final Exam-scheduled by Student Records (3 hours): | 35% |
Lab Report Format:
Lab reports should be written in the style of a research paper
published in a scientific journal, e.g. Biochemistry. Typically,
your lab report will be subdivided into Introduction, Materials
and Methods, Results, Discussion, and References. Don't recopy
the lab handouts in your lab report.
Final Exam:
Examinable material includes that presented in 4700 workshops, and experiments, including handout material and problem sets, and closely related material presented in the 4630 lectures; for example, the lectures on circular dichroism, NMR, mass spectrometry etc. Some problem set material will appear on the final exam.
Policy:
- Unless a medical certificate is presented attendance at all labs is mandatory.
Lab reports will not be accepted from anyone not attending a lab.
- Lab reports are due 3 weeks after the day
the experiment was completed.
- Late penalty is 20%.
- Research proposal (see below) due: Tuesday March 13.
- Penalty for late papers is 10% per day.
Final Exam Review
Students in the Faculty of Science are permitted to review their final exams before the deadline for appealing final grades (Final Grade Appeal). If you wish to view your final exam please go to the Department of Chemistry general office (360 Parker Building), fill in an application form, and pay the $5.00 fee.
Academic Dishonesty: Please visit the Faculty of Science web site Cheating, Plagiarism etc.
Research Proposal:
Due Tuesday March 13.
Read the paper by Oloo et al., (2011 J. Biol. Chem. 286, 12133 - 40) entitled "Structure-guided antigen engineering yields pneumolysin mutants suitable for vaccination against pneumococcal disease." Then, suppose you are preparing an application to a granting agency. Write a research proposal describing how you would extend the research described in this paper. You will notice that the authors apply a number of methods that are not covered in our course. You might propose to apply one or more of their methods to a different protein, or you might prefer to apply one of the methods used in our course to Pneumolysin or another protein. As long as there is some connection to the paper by Oloo et al., any proposal will be acceptable. Your approach may be pure, research driven aimed at learning more about a protein, or it might be more applied, with a human health goal similar to the approach of Oloo and colleagues. It is a good idea to state a formal hypothesis and then explain how you will test your hypothesis rather than simply proposing to collect data. Don't restrict yourself to using only the methods used by Oloo et al. Remember, your experiments should be scientifically sound. That does not mean you must be sure they would work; research projects often do not work! You will be judged mainly on the scientific merit of your proposal but organization, spelling, and grammar will also count.
Length:
2,000-3,000 words, typed or very neatly handwritten. Do not write a chapter for a book; most grant application forms provide only limited space for a proposal. Diagrams are usually helpful.
References:
Use the format of one of the above papers; put references at the end and refer properly in the text.
Useful WWW Sites for CHEM 4700:
Return to the Chemistry Department Course Descriptions
Return to Joe O'Neil's Home Page
http://home.cc.umanitoba.ca/~joneil/CHEM4700.Course.outline.htm
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