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Hélène
Perreault
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Room
517 Parker Building
Tel (204) 474-7418
Fax (204) 474-7608
E-mail perreau@cc.umanitoba.ca
RESEARCH
INTERESTS
Bioanalytical Chemistry
Proteins play very
important roles in all types of organisms. They can act as enzymes, catalysts,
oxygen carriers, structure building blocks, among many other possible roles.
Normal protein function results of a combination of amino acid sequence, 3-D
structure and sometimes aggregation with other proteins or biomolecules. If
sequence (thus often structure) is altered, protein function changes, possibly
causing diseases or other problems.
Over the past ten
years, we have been interested in characterizing modified proteins from several
sources, using mass spectrometry as our main analytical tool. Because proteins
in cells always occur in very complex mixtures, preliminary steps such as
electrophoresis and chromatography are often required in sample preparation.
The mass spectrometers in our laboratory are equipped with a range of
ionization methods, e.g. electrospray (ES) and matrix-assisted laser
desorption/ionization (MALDI). Our research group now counts four graduate
students, a technician and a research associate. See a list or recent
publications below.
Fragmentation
of a glycopeptide
At this stage, we have projects on the characterization of glycoproteins, phosphoproteins, modified enzymes. We work in collaboration with medical researchers, microbiologists, biochemists and industry researchers. We also analyze samples as a service to our Department and to the scientific community.
Sequencing a peptide by tandem mass spectrometry
To learn more about our group and laboratory: Visit the Mass
Spectrometry Resource Centre Home Page.
TEACHING: Bioanalytical Chemistry
CHEM
4590, Advances Bioanalytical Chemistry: This
is a half-year, 3 credit-hour fall course. Visit the course web
site for a tentative course schedule.
RESEARCH
PUBLICATIONS, 2005 -
S.I.
Snovida, H. Perreault, 2007, "A 2,5-Dihydroxybenzoic
Acid/N,N-Dimethylaniline Matrix for the Analysis of Oligosaccharides by
Matrix-Assisted Laser Desorption Ionization Mass Spectrometry", Rapid
Commun. Mass Spectrom., 21, 3711-3715.
E.
Lattová, V. C. Chen, S. Davda, T. Bezabeh, H. Perreault, 2007,
"MALDI On-Target Method for the Investigation of Oligosaccharides and
Glycosylation Sites in Glycopeptides and Glycoproteins", Rapid Commun.
Mass Spectrom. 21, 1644-50.
Y.
Wada, P. Azadi, C. E. Costello, A. Dell, R. A. Dwek, H. Geyer, R. Geyer, K.
Kakehi, N. G. Karlsson, K. Kato, N. Kawasaki, K.-H. Khoo, S. Kim, A. Kondo, E.
Lattova, Y. Mechref, E. Miyoshi, K. Nakamura, H. Narimatsu, M. V. Novotny, N.
H. Packer, H. Perreault, J. Peter-Katalinić, G. Pohlentz, V. N. Reinhold,
P. M. Rudd, A. Suzuki, N. Taniguchi, 2007, "Comparison of the Methods for Profiling Glycoprotein
Glycans: HUPO HGPI (Human Proteome Organisation Human Disease
Glycomics/Proteome Initiative) Multi-institutional Study", Glycobiology, 17, 411-422.
S. I. Snovida, V. C. Chen, H. Perreault, 2006, "Use of a 2,5-Dihydroxybenzoic Acid/Aniline MALDI
Matrix for Improved Detection and On-Target Derivatization of Glycans: A
Preliminary Report", Anal. Chem. 78, 8561-8568.
P. Kapková,
V.
C. Chen, X. Li, H. Perreault, J.I. Nagy, 2006, “Interaction of Zonula
Occludens-1 (ZO-1) with α-Actinin-4: Application of Functional Proteomics
for Identification of PDZ Domain-Associated Proteins", Journal of Proteome
Research 5, 2123-2134.
S. I. Snovida, V. C. Chen, O. Krokhin, H. Perreault, 2006,
Isolation and Identification of Sialylated Glycopeptides from Bovine
α1-acid Glycoprotein by Off-line Capillary Electrophoresis MALDI-TOF Mass
Spectrometry, Anal. Chem. 78, 6556-6563.
V. Restelli, M. D. Wang, N. Huzel, M. Ethier, H. Perreault,
M. Butler, 2006, “The
effect of dissolved oxygen on the production and the glycosylation profile of
recombinant human erythropoietin produced from CHO cells”, Biotech.
Bioeng., 94(3), 481-494.
Glycopeptides: Direct Determination of Glycosylation
Sites in Proteins”, Anal. Chem. 78, 2977-2984.
M.
Ethier, D. Figeys and H. Perreault, 2006, “N-Glycosylation Analysis Using
the StrOligo Algorithm”, in “New and Emerging Proteomics Techniques”, D. Nedelkov and
R.W. Nelson eds, Humana Press, Totowa, New Jersey, ISBN 1-59745-026-X.
M.
Ethier, O. Krokhin, W. Ens, K. G. Standing, J.A. Wilkins and H. Perreault,
2005, “Global and Site-Specific Detection of Human Integrin a5b1
Glycosylation Using Tandem Mass Spectrometry and the StrOligo Algorithm”,
Rapid Commun. Mass Spectrom. 19,
721-727.
E. Lattova, S. Snovida, O. Krokhin,
H. Perreault, 2005, “Influence of
the Labeling Group on Ionization and Fragmentation
of Carbohydrates in Mass
Spectrometry”, J.
Am. Soc. Mass Spectrom. 16, 683-696.
Go to the Chemistry Department page
Last updated September 1, 2009 by H. Perreault