CLASSIFICATION OF TWO-FACTOR DATA
CLASSIFICATION OF TWO-FACTOR DATA Jonathan Hodgkin 21/4/93
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Phenotypes: WT = wild type
nonWT = viable non-wild-type,
ALL = total viable progeny
X = x/x and xy/x+, Y = y/y and xy/+y
XY = xy/xy
D = d/+ and d/d, dx/+ etc
H = xy/x+
Type Phenotypes Calculation
A. Recessive markers x and y, segregation from xy/++ hermaphrodites
1. Full WT X Y XY (2p-p^2)/2 = (X+Y)/(WT+X+Y+XY)
2. One_recombinant WT X (2p-p^2)/3 = X/(WT+X)
3. Selected X XY (2p-p^2) = X/(X+XY)
4. One_all X ALL (2p-p^2)/4 = X/ALL
5. Recs_all X Y ALL (2p-p^2)/2 = (X+Y)/ALL
A' Recessive markers x and y, segregation from xy+/++z, where z is
tightly linked and z/z is lethal or otherwise not scored.
6. One_let X ALL (2p-p^2)/2 = X/ALL
B Recessive markers, segregation from x+/+y hermaphrodites
7. Tested H X 2p/(1+p) = H/X
8. Selected_trans X XY p^2 = XY/(X+XY)
C Recessive markers, scored by backcross: xy/++ males crossed with
xy/xy hermaphrodites. Usually only males scored.
9. Backcross WT X Y XY p = (X+Y)/(WT+X+Y+XY)
10. Back_one WT X p = X/(WT+X)
D Recessive sex-linked markers, x+/+y hermaphrodites crossed with wild
males, male progeny scored.
11. Sex_all WT X Y XY p = (WT+XY)/(WT+X+Y+XY)
12. Sex_one WT X p = WT/(WT+X)
D' Recessive sex-linked markers, xy/++ hermaphrodites crossed with wild
males, male progeny scored.
13. Sex_cis X ALL p/2 = X/ALL
E One marker (d) is dominant or semidominant. Segregation from x+/+d
hermaphrodites. For dom_semi, D = d/+, not d/d (as in sqt)
14. Dom_one WT nonWT (2p-p^2)/4 = WT/(WT+nonWT)
15. Dom_selected WT X (2p-p^2) = WT/(WT+X)
16. Dom_semi XD ALL (p-p^2)/2 = XD/ALL
E' One marker (d) is dominant, and either d or x is recessive lethal
17. Dom_let WT ALL (2p-p^2)/3 = WT/ALL
F Direct: single progeny picked and tested for genotype
on both chromosomes
18. Direct R = # recombinant chromosomes p = R/T
T = # total chromosomes
G Complex:
19. Complex_mixed X ALL (2p-p^2)/5 = X/ALL
H Segregation from lethal heterozygote, x +/+ y, where y is lethal; WT
progeny picked, N = number of these failing to segregate x.
20. Lethal_tested N WT (2p-p^2)/(2+p^2) = N/WT