#maximal read length max_rd_len=100 [LIB] #average insert size avg_ins=200 #if sequence needs to be reversed reverse_seq=0 #in which part(s) the reads are used asm_flags=3 #use only first 100 bps of each read rd_len_cutoff=100 #in which order the reads are used while scaffolding rank=1 # cutoff of pair number for a reliable connection (at least 3 for short insert size) pair_num_cutoff=3 #minimum aligned length to contigs for a reliable read location (at least 32 for short insert size) map_len=32 #a pair of fastq file, read 1 file should always be followed by read 2 file q1=/path/**LIBNAMEA**/fastq1_read_1.fq q2=/path/**LIBNAMEA**/fastq1_read_2.fq #another pair of fastq file, read 1 file should always be followed by read 2 file q1=/path/**LIBNAMEA**/fastq2_read_1.fq q2=/path/**LIBNAMEA**/fastq2_read_2.fq #a pair of fasta file, read 1 file should always be followed by read 2 file f1=/path/**LIBNAMEA**/fasta1_read_1.fa f2=/path/**LIBNAMEA**/fasta1_read_2.fa #another pair of fasta file, read 1 file should always be followed by read 2 file f1=/path/**LIBNAMEA**/fasta2_read_1.fa f2=/path/**LIBNAMEA**/fasta2_read_2.fa #fastq file for single reads q=/path/**LIBNAMEA**/fastq1_read_single.fq #another fastq file for single reads q=/path/**LIBNAMEA**/fastq2_read_single.fq #fasta file for single reads f=/path/**LIBNAMEA**/fasta1_read_single.fa #another fasta file for single reads f=/path/**LIBNAMEA**/fasta2_read_single.fa #a single fasta file for paired reads p=/path/**LIBNAMEA**/pairs1_in_one_file.fa #another single fasta file for paired reads p=/path/**LIBNAMEA**/pairs2_in_one_file.fa #bam file for single or paired reads, reads 1 in paired reads file should always be followed by reads 2 # NOTE: If a read in bam file fails platform/vendor quality checks(the flag field 0x0200 is set), itself and it's paired read would be ignored. b=/path/**LIBNAMEA**/reads1_in_file.bam #another bam file for single or paired reads b=/path/**LIBNAMEA**/reads2_in_file.bam [LIB] avg_ins=2000 reverse_seq=1 asm_flags=2 rank=2 # cutoff of pair number for a reliable connection (at least 5 for large insert size) pair_num_cutoff=5 #minimum aligned length to contigs for a reliable read location (at least 35 for large insert size) map_len=35 q1=/path/**LIBNAMEB**/fastq_read_1.fq q2=/path/**LIBNAMEB**/fastq_read_2.fq f1=/path/**LIBNAMEA**/fasta_read_1.fa f2=/path/**LIBNAMEA**/fasta_read_2.fa p=/path/**LIBNAMEA**/pairs_in_one_file.fa b=/path/**LIBNAMEA**/reads_in_file.bam