Blixem - display multiple alignments against a reference sequence. Usage: blixem [options] [] [X options] contains the reference sequence in FASTA format. is a GFF v3 file containing alignments and other features. If is ommitted, should contain the reference sequence in FASTA format, below a comment line that reads ##FASTA. Both and can be substituted by "-" for reading from stdin (pipe). If is piped, the first line should contain the sequence name and the second the sequence itself. Options: -t , --display-type= MANDATORY Whether to display sequences in nucleotide or protein mode. Must be one of: N = nucleotide P = protein -a , --alignment-names= Specify a string giving the names of the alignments, e.g. "EST_mouse EST_human" etc. -c , --config-file= Read configuration options from 'file'. --compiled Show package compile date. --dataset Optional string to indicate a data-set that the alignments are from. --dotter-first-match Call Dotter on the first match to the right of the default start coord. --fetch-server Causes Blixem to get sequences from a fetch server at machine 'nodeid' on the given port (default 22100). -h, --help More detailed usage information. --hide-big-picture Hide the big picture section on start-up. --hide-inactive-strand Hide the inactive strand (i.e. the reverse strand, or the forward strand if the -R option is used). --highlight-diffs Enable 'highlight differences' mode, where mismatches (rather than matches) are highlighted. --invert-sort Invert sorting order -m , --map-coords= Map the coordinate system so that the given 'from' coordinate maps to the given 'to' coordinate (or to '1' if 'to' is not given). -n, --negate-coords When showing the reverse strand, negate the display coordinates. -o , --offset= Offset the reference sequence coordinate system by n. --optional-data Parse additional data such as organism and tissue-type on start-up. --remove-input-files Delete the input files after they have been parsed. -r, --reverse-strand Indicates that the given reference sequence is the reverse strand. --save-temp-files Save any temporary files created by Blixem. --show-coverage Display the coverage section on start-up. --sort-mode= Default sort mode. Use --help option to see details. --squash-matches Compress the alignment lists on start-up. -s , --start-coord= Start with the display centred on coordinate n. --start-next-match Start with the display centred on the first match to the right of the default start coord. --styles-file= Read color options from a key-value file. Use --help option to see details. --version Show package version number. -z , --zoom-range= Specify the initial range of coordinates to zoom the big picture in to. --zoom-whole Start with the big picture zoomed out to view the full reference sequence range. Some X options: -acefont Main font. -font Menu font. ----- Written by Gemma Barson Based on original code by Erik Sonnhammer Reference: Sonnhammer ELL & Durbin R (1994). A workbench for Large Scale Sequence Homology Analysis. Comput. Applic. Biosci. 10:301-307. See http://www.sanger.ac.uk/resources/software/seqtools/ for more info. Copyright (c) 2009-2011: Genome Research Ltd. Blixem is distributed under the GNU Public License; see http://www.gnu.org/copyleft/gpl.txt Version 4.10-1-g6bff 17:38:25 Apr 4 2012