#include "mltaln.h" #include "dp.h" #define DEBUG 0 #define XXXXXXX 0 #define USE_PENALTY_EX 1 #define TERMGAPFAC 0.0 #define TERMGAPFAC_EX 0.0 #if 1 #if 0 static void match_calc_mtx_codon( double **codonmtx, double **mtx, double *match, char **s1, char **s2, int **codonseq1, int **codonseq2, int i1, int lgth2 ) // gstart, gend wo tsukatte coding ka kakuninn suru beki { char *seq2 = s2[0]; double *doubleptr = mtx[(unsigned char)s1[0][i1]]; int codonid1, codonid2; // int codonid1p, codonid2p; // int codonid1pp, codonid2pp; double codonmatch; // int *pt0, *pt1, *pt2; int *pt0; codonid1 = codonseq1[0][i1]; // codonid1p = codonseq1[1][i1]; // codonid1pp = codonseq1[2][i1]; pt0 = codonseq2[0]; // pt1 = codonseq2[1]; // pt2 = codonseq2[2]; // posin2 = 0; while( lgth2-- ) { // reporterr( "%c-%c -> %f\n", s1[0][i1], *seq2, doubleptr[(unsigned char)*seq2] ); // track ha at de kokoromiru codonid2 = *pt0++; // codonid2p = *pt1++; // codonid2pp = *pt2++; // posin2++; // // reporterr( "%c%c%c(%d)-%c%c%c(%d)\n", s1[0][i1], s1[0][i1+1], s1[0][i1+2], codonid1, *seq2, *(seq2+1), *(seq2+2), codonid2 ); codonmatch = 0.0; if( codonid1 > -1 && codonid2 > -1 ) codonmatch = codonmtx[codonid1][codonid2] * 600.0; // if( codonid1p > -1 && codonid2p > -1 ) codonmatch -= codonmtx[codonid1p][codonid2p] * 600; // if( codonid1pp > -1 && codonid2pp > -1 ) codonmatch -= codonmtx[codonid1pp][codonid2pp] * 600; *match++ = doubleptr[(unsigned char)*seq2++] + codonmatch; } } #else static void match_calc_mtx_codon( double **codonmtx, double **mtx, double *match, char **s1, char **s2, int **codonseq1, int **codonseq2, int i1, int lgth2 ) // gstart, gend wo tsukatte coding ka kakuninn suru beki { char *seq2 = s2[0]; double *doubleptr = mtx[(unsigned char)s1[0][i1]]; int codonid1, codonid2; double codonmatch; int *pt0; codonid1 = codonseq1[0][i1]; pt0 = codonseq2[0]; while( lgth2-- ) { // reporterr( "%c-%c -> %f\n", s1[0][i1], *seq2, doubleptr[(unsigned char)*seq2] ); codonid2 = *pt0++; // reporterr( "%c%c%c(%d)-%c%c%c(%d)\n", s1[0][i1], s1[0][i1+1], s1[0][i1+2], codonid1, *seq2, *(seq2+1), *(seq2+2), codonid2 ); codonmatch = 0.0; if( codonid1 > -1 && codonid2 > -1 ) codonmatch = codonmtx[codonid1][codonid2] * 600.0; // codonid1>-1 ha iranai. *match++ = doubleptr[(unsigned char)*seq2++] + codonmatch; } } #endif static void match_calc_mtx( double **mtx, double *match, char **s1, char **s2, int i1, int lgth2 ) { char *seq2 = s2[0]; double *doubleptr = mtx[(unsigned char)s1[0][i1]]; while( lgth2-- ) *match++ = doubleptr[(unsigned char)*seq2++]; } #else static void match_calc( double *match, char **s1, char **s2, int i1, int lgth2 ) { int j; for( j=0; j= warpbase ) if( tailgp == 1 ) ; else { #if 1 // reporterr( "lastverticalw[lgth1-1] = %f\n", lastverticalw[lgth1-1] ); // reporterr( "lasthorizontalw[lgth2-1] = %f\n", lasthorizontalw[lgth2-1] ); wm = lasthorizontalw[lgth2-1] - 1.0; // lasthorizontalw[lgth2-1] yori kanarazu chiisai. for( j=lgth2-2; j>=0; j-- ) { if( (g=lasthorizontalw[j]+ ( fpenalty * TERMGAPFAC + fpenalty_ex * (lgth2-1-j) * TERMGAPFAC_EX ) ) > wm ) { wm = g; iin = lgth1-1; jin = j; ijp[lgth1][lgth2] = -( lgth2 - j ); } } for( i=lgth1-2; i>=0; i-- ) { if( ( g=lastverticalw[i]+ ( fpenalty * TERMGAPFAC + fpenalty_ex * (lgth1-1-i) * TERMGAPFAC_EX ) ) > wm ) { wm = g; iin = i; jin = lgth2-1; ijp[lgth1][lgth2] = +( lgth1 - i ); } } if( lasthorizontalw[lgth2-1] > wm ) // score ga onaji baai erabarenai { wm = lasthorizontalw[lgth2-1]; iin = lgth1-1; jin = lgth2-1; ijp[lgth1][lgth2] = 0; } #else wm = lastverticalw[0]; for( i=0; i= wm ) { wm = lastverticalw[i]; iin = i; jin = lgth2-1; ijp[lgth1][lgth2] = +( lgth1 - i ); } } for( j=0; j= wm ) { wm = lasthorizontalw[j]; iin = lgth1-1; jin = j; ijp[lgth1][lgth2] = -( lgth2 - j ); } } #endif } mseq1[0] += lgth1+lgth2; *mseq1[0] = 0; mseq2[0] += lgth1+lgth2; *mseq2[0] = 0; iin = lgth1; jin = lgth2; limk = lgth1+lgth2 + 1; for( k=0; k= warpbase ) { // fprintf( stderr, "WARP!\n" ); ifi = warpis[ijp[iin][jin]-warpbase]; jfi = warpjs[ijp[iin][jin]-warpbase]; } else if( ijp[iin][jin] < 0 ) { ifi = iin-1; jfi = jin+ijp[iin][jin]; } else if( ijp[iin][jin] > 0 ) { ifi = iin-ijp[iin][jin]; jfi = jin-1; } else { ifi = iin-1; jfi = jin-1; } if( ifi == -warpbase && jfi == -warpbase ) { l = iin; while( --l >= 0 ) { *--mseq1[0] = seq1[0][l]; *--mseq2[0] = *gap; k++; } l= jin; while( --l >= 0 ) { *--mseq1[0] = *gap; *--mseq2[0] = seq2[0][l]; k++; } break; } else { l = iin - ifi; while( --l > 0 ) { *--mseq1[0] = seq1[0][ifi+l]; *--mseq2[0] = *gap; k++; } l= jin - jfi; while( --l > 0 ) { *--mseq1[0] = *gap; *--mseq2[0] = seq2[0][jfi+l]; k++; } } if( iin <= 0 || jin <= 0 ) break; *--mseq1[0] = seq1[0][ifi]; *--mseq2[0] = seq2[0][jfi]; k++; iin = ifi; jin = jfi; } // fprintf( stderr, "%s\n", mseq1[0] ); // fprintf( stderr, "%s\n", mseq2[0] ); return( wm ); } double G__align11psg( double **codonmtx, double **n_dynamicmtx, char **seq1, char **seq2, int alloclen, int headgp, int tailgp, double *gstart, double *gend ) { // int k; register int i, j; int lasti; /* outgap == 0 -> lgth1, outgap == 1 -> lgth1+1 */ int lastj; int lgth1, lgth2; int resultlen; double wm, wmo; /* int ?????? */ double g; double *currentw, *previousw; double fpenalty = (double)penalty; double fpenalty_shift = (double)penalty_shift; double fpenalty_tmp; #if USE_PENALTY_EX double fpenalty_ex = (double)penalty_ex; double fpenalty_ex_i; #endif #if 1 double *wtmp; int *ijppt; double *mjpt, *prept, *curpt; int *mpjpt; #endif static TLS double mi = 0.0; static TLS double *m = NULL; static TLS int **ijp = NULL; static TLS int mpi = 0; static TLS int *mp = NULL; static TLS double *w1 = NULL; static TLS double *w2 = NULL; static TLS double *match = NULL; static TLS double *initverticalw = NULL; /* kufuu sureba iranai */ static TLS double *lastverticalw = NULL; /* kufuu sureba iranai */ static TLS char **mseq1 = NULL; static TLS char **mseq2 = NULL; static TLS char **mseq = NULL; static TLS int **intwork = NULL; static TLS double **doublework = NULL; static TLS int orlgth1 = 0, orlgth2 = 0; static TLS double **amino_dynamicmtx = NULL; // ?? static TLS int **codonseq1 = NULL; static TLS int **codonseq2 = NULL; int *warpis = NULL; int *warpjs = NULL; int *warpi = NULL; int *warpj = NULL; int *prevwarpi = NULL; int *prevwarpj = NULL; double *wmrecords = NULL; double *prevwmrecords = NULL; int warpn = 0; int warpbase; double curm = 0.0; double *wmrecordspt, *wmrecords1pt, *prevwmrecordspt; int *warpipt, *warpjpt; if( seq1 == NULL ) { if( orlgth1 > 0 && orlgth2 > 0 ) { orlgth1 = 0; orlgth2 = 0; if( mseq1 ) free( mseq1 ); mseq1 = NULL; if( mseq2 ) free( mseq2 ); mseq2 = NULL; if( w1 ) FreeFloatVec( w1 ); w1 = NULL; if( w2 ) FreeFloatVec( w2 ); w2 = NULL; if( match ) FreeFloatVec( match ); match = NULL; if( initverticalw ) FreeFloatVec( initverticalw ); initverticalw = NULL; if( lastverticalw ) FreeFloatVec( lastverticalw ); lastverticalw = NULL; if( m ) FreeFloatVec( m ); m = NULL; if( mp ) FreeIntVec( mp ); mp = NULL; if( mseq ) FreeCharMtx( mseq ); mseq = NULL; if( doublework ) FreeFloatMtx( doublework ); doublework = NULL; if( intwork ) FreeIntMtx( intwork ); intwork = NULL; if( amino_dynamicmtx ) FreeDoubleMtx( amino_dynamicmtx ); amino_dynamicmtx = NULL; if( codonseq1 ) FreeIntMtx( codonseq1 ); codonseq1 = NULL; if( codonseq2 ) FreeIntMtx( codonseq2 ); codonseq2 = NULL; } orlgth1 = 0; orlgth2 = 0; return( 0.0 ); } lgth1 = strlen( seq1[0] ); lgth2 = strlen( seq2[0] ); warpbase = lgth1 + lgth2; warpis = NULL; warpjs = NULL; warpn = 0; if( trywarp ) { // fprintf( stderr, "IN G__align11\n" ); if( headgp == 0 || tailgp == 0 ) { fprintf( stderr, "At present, headgp and tailgp must be 1.\n" ); exit( 1 ); } wmrecords = AllocateFloatVec( lgth2+1 ); warpi = AllocateIntVec( lgth2+1 ); warpj = AllocateIntVec( lgth2+1 ); prevwmrecords = AllocateFloatVec( lgth2+1 ); prevwarpi = AllocateIntVec( lgth2+1 ); prevwarpj = AllocateIntVec( lgth2+1 ); for( i=0; i orlgth1 || lgth2 > orlgth2 ) { int ll1, ll2; if( orlgth1 > 0 && orlgth2 > 0 ) { FreeFloatVec( w1 ); FreeFloatVec( w2 ); FreeFloatVec( match ); FreeFloatVec( initverticalw ); FreeFloatVec( lastverticalw ); FreeFloatVec( m ); FreeIntVec( mp ); FreeCharMtx( mseq ); FreeFloatMtx( doublework ); FreeIntMtx( intwork ); FreeDoubleMtx( amino_dynamicmtx ); if( codonseq1 ) FreeIntMtx( codonseq1 ); if( codonseq2 ) FreeIntMtx( codonseq2 ); } ll1 = MAX( (int)(1.3*lgth1), orlgth1 ) + 100; ll2 = MAX( (int)(1.3*lgth2), orlgth2 ) + 100; #if DEBUG fprintf( stderr, "\ntrying to allocate (%d+%d)xn matrices ... ", ll1, ll2 ); #endif w1 = AllocateFloatVec( ll2+2 ); w2 = AllocateFloatVec( ll2+2 ); match = AllocateFloatVec( ll2+2 ); initverticalw = AllocateFloatVec( ll1+2 ); lastverticalw = AllocateFloatVec( ll1+2 ); m = AllocateFloatVec( ll2+2 ); mp = AllocateIntVec( ll2+2 ); mseq = AllocateCharMtx( 2, ll1+ll2 ); // 2020/Apr doublework = AllocateFloatMtx( nalphabets, MAX( ll1, ll2 )+2 ); intwork = AllocateIntMtx( nalphabets, MAX( ll1, ll2 )+2 ); amino_dynamicmtx = AllocateDoubleMtx( 0x100, 0x100 ); if( codonscore ) { codonseq1 = AllocateIntMtx( 3, ll1 ); // Only codonseq1[0] is used at this point codonseq2 = AllocateIntMtx( 3, ll2 ); // Only codonseq2[0] is used at this point } #if DEBUG fprintf( stderr, "succeeded\n" ); #endif orlgth1 = ll1 - 100; orlgth2 = ll2 - 100; } for( i=0; i commonAlloc1 || orlgth2 > commonAlloc2 ) { int ll1, ll2; if( commonAlloc1 && commonAlloc2 ) { FreeIntMtx( commonIP ); } ll1 = MAX( orlgth1, commonAlloc1 ); ll2 = MAX( orlgth2, commonAlloc2 ); #if DEBUG fprintf( stderr, "\n\ntrying to allocate %dx%d matrices ... ", ll1+1, ll2+1 ); #endif commonIP = AllocateIntMtx( ll1+10, ll2+10 ); #if DEBUG fprintf( stderr, "succeeded\n\n" ); #endif commonAlloc1 = ll1; commonAlloc2 = ll2; } ijp = commonIP; if( codonscore ) { // codonseq[1..2] are not used yet. for( i=0; i<3&&i 0.5 ) // only at 3rd position match_calc_mtx_codon( codonmtx, amino_dynamicmtx, currentw, seq1, seq2, codonseq1, codonseq2, 0, lgth2 ); else match_calc_mtx( amino_dynamicmtx, currentw, seq1, seq2, 0, lgth2 ); #else match_calc_mtx_codon( codonmtx, amino_dynamicmtx, currentw, seq1, seq2, codonseq1, codonseq2, 0, lgth2, gstart[0] == 0.5 && gend[0] > 0.5 ); #endif } else { match_calc_mtx( amino_dynamicmtx, initverticalw, seq2, seq1, 0, lgth1 ); match_calc_mtx( amino_dynamicmtx, currentw, seq1, seq2, 0, lgth2 ); } if( headgp == 1 ) { for( i=1; i 0.5 ) ) // only at 3rd position in seq1 match_calc_mtx_codon( codonmtx, amino_dynamicmtx, currentw, seq1, seq2, codonseq1, codonseq2, i, lgth2 ); else match_calc_mtx( amino_dynamicmtx, currentw, seq1, seq2, i, lgth2 ); #else if( codonscore ) // only at 3rd position in seq1 match_calc_mtx_codon( codonmtx, amino_dynamicmtx, currentw, seq1, seq2, codonseq1, codonseq2, i, lgth2, gstart[i] == 0.5 && gend[i] > 0.5 ); else match_calc_mtx( amino_dynamicmtx, currentw, seq1, seq2, i, lgth2 ); #endif #if XXXXXXX fprintf( stderr, "\n" ); fprintf( stderr, "i=%d\n", i ); fprintf( stderr, "currentw = \n" ); for( j=0; j", wm ); #endif #if 0 fprintf( stderr, "%5.0f?", g ); #endif if( (g=mi+fpenalty*gend[i]) > wm ) { wm = g; *ijppt = -( j - mpi ); // reporterr( "hit! jump from %d to %d: %c->%c\n", j, mpi, seq2[0][j], seq2[0][mpi] ); } if( (g=*prept+fpenalty*gstart[i-1]) >= mi ) // if( (g=*prept) > mi ) { mi = g; mpi = j-1; // reporterr( "hit! jump to %d: ->%c:%c\n", mpi, seq1[0][i-1], seq2[0][mpi] ); } #if USE_PENALTY_EX mi += fpenalty_ex_i; // mi += fpenalty_ex; #endif #if 0 fprintf( stderr, "%5.0f?", g ); #endif if( (g=*mjpt + fpenalty*gend[i]) > wm ) { wm = g; *ijppt = +( i - *mpjpt ); } if( (g=*prept+fpenalty*gstart[i-1]) >= *mjpt ) // if( (g=*prept) > *mjpt ) { *mjpt = g; *mpjpt = i-1; } #if USE_PENALTY_EX if( j < lgth2 ) // 2018/May/11 m[j] += fpenalty_ex; #endif #if 1 if( trywarp ) { fpenalty_tmp = fpenalty_shift + fpenalty_ex * ( i - prevwarpi[j-1] + j - prevwarpj[j-1] ); // fprintf( stderr, "fpenalty_shift = %f\n", fpenalty_tmp ); // fprintf( stderr, "\n\n\nwarp to %c-%c (%d-%d) from %c-%c (%d-%d) ? prevwmrecords[%d] = %f + %f <- wm = %f\n", seq1[0][prevwarpi[j-1]], seq2[0][prevwarpj[j-1]], prevwarpi[j-1], prevwarpj[j-1], seq1[0][i], seq2[0][j], i, j, j, prevwmrecords[j-1], fpenalty_tmp, wm ); // if( (g=prevwmrecords[j-1] + fpenalty_shift )> wm ) if( ( g=*prevwmrecordspt++ + fpenalty_tmp )> wm ) // naka ha osokute kamawanai { // fprintf( stderr, "Yes! Warp!! from %d-%d (%c-%c) to %d-%d (%c-%c) fpenalty_tmp = %f! warpn = %d\n", i, j, seq1[0][i], seq2[0][j-1], prevwarpi[j-1], prevwarpj[j-1],seq1[0][prevwarpi[j-1]], seq2[0][prevwarpj[j-1]], fpenalty_tmp, warpn ); if( warpn && prevwarpi[j-1] == warpis[warpn-1] && prevwarpj[j-1] == warpjs[warpn-1] ) { *ijppt = warpbase + warpn - 1; } else { *ijppt = warpbase + warpn; warpis = realloc( warpis, sizeof(int) * ( warpn+1 ) ); warpjs = realloc( warpjs, sizeof(int) * ( warpn+1 ) ); warpis[warpn] = prevwarpi[j-1]; warpjs[warpn] = prevwarpj[j-1]; warpn++; } wm = g; } else { } curm = *curpt + wm; // fprintf( stderr, "###### curm = %f at %c-%c, i=%d, j=%d\n", curm, seq1[0][i], seq2[0][j], i, j ); // fprintf( stderr, "copy from i, j-1? %f > %f?\n", wmrecords[j-1], curm ); // if( wmrecords[j-1] > wmrecords[j] ) if( *wmrecords1pt > *wmrecordspt ) { // fprintf( stderr, "yes\n" ); // wmrecords[j] = wmrecords[j-1]; *wmrecordspt = *wmrecords1pt; // warpi[j] = warpi[j-1]; // warpj[j] = warpj[j-1]; *warpipt = *(warpipt-1); *warpjpt = *(warpjpt-1); // fprintf( stderr, "warpi[j]=%d, warpj[j]=%d wmrecords[j] = %f\n", warpi[j], warpj[j], wmrecords[j] ); } // else // { // fprintf( stderr, "no\n" ); // } // fprintf( stderr, " curm = %f at %c-%c\n", curm, seq1[0][i], seq2[0][j] ); // fprintf( stderr, " wmrecords[%d] = %f\n", j, wmrecords[j] ); // fprintf( stderr, "replace?\n" ); // if( curm > wmrecords[j] ) if( curm > *wmrecordspt ) { // fprintf( stderr, "yes at %d-%d (%c-%c), replaced warp: warpi[j]=%d, warpj[j]=%d warpn=%d, wmrecords[j] = %f -> %f\n", i, j, seq1[0][i], seq2[0][j], i, j, warpn, wmrecords[j], curm ); // wmrecords[j] = curm; *wmrecordspt = curm; // warpi[j] = i; // warpj[j] = j; *warpipt = i; *warpjpt = j; } // else // { // fprintf( stderr, "No! warpi[j]=%d, warpj[j]=%d wmrecords[j] = %f\n", warpi[j], warpj[j], wmrecords[j] ); // } // fprintf( stderr, "%d-%d (%c-%c) curm = %5.0f, wmrecords[j]=%f\n", i, j, seq1[0][i], seq2[0][j], curm, wmrecords[j] ); wmrecordspt++; wmrecords1pt++; warpipt++; warpjpt++; } #endif *curpt++ += wm; ijppt++; mjpt++; prept++; mpjpt++; } lastverticalw[i] = currentw[lgth2-1]; // lgth2==0 no toki error if( trywarp ) { fltncpy( prevwmrecords, wmrecords, lastj ); intncpy( prevwarpi, warpi, lastj ); intncpy( prevwarpj, warpj, lastj ); } } if( trywarp ) { // fprintf( stderr, "\nwm = %f\n", wm ); // fprintf( stderr, "warpn = %d\n", warpn ); free( wmrecords ); free( prevwmrecords ); free( warpi ); free( warpj ); free( prevwarpi ); free( prevwarpj ); } wmo = Atracking( currentw, lastverticalw, seq1, seq2, mseq1, mseq2, ijp, tailgp, warpis, warpjs, warpbase ); if( !tailgp ) wm = wmo; // reporterr( "wm (after tracking) = %f\n", wm ); if( warpis ) free( warpis ); if( warpjs ) free( warpjs ); resultlen = strlen( mseq1[0] ); if( alloclen < resultlen || resultlen > N ) { fprintf( stderr, "alloclen=%d, resultlen=%d, N=%d\n", alloclen, resultlen, N ); ErrorExit( "LENGTH OVER!\n" ); } strcpy( seq1[0], mseq1[0] ); strcpy( seq2[0], mseq2[0] ); #if 0 fprintf( stderr, "\n" ); fprintf( stderr, ">\n%s\n", mseq1[0] ); fprintf( stderr, ">\n%s\n", mseq2[0] ); fprintf( stderr, "wm = %f\n", wm ); #endif return( wm ); } double G__align11( double **n_dynamicmtx, char **seq1, char **seq2, int alloclen, int headgp, int tailgp ) { // int k; register int i, j; int lasti; /* outgap == 0 -> lgth1, outgap == 1 -> lgth1+1 */ int lastj; int lgth1, lgth2; int resultlen; double wm, wmo; /* int ?????? */ double g; double *currentw, *previousw; double fpenalty = (double)penalty; double fpenalty_shift = (double)penalty_shift; double fpenalty_tmp; #if USE_PENALTY_EX double fpenalty_ex = (double)penalty_ex; double fpenalty_ex_i; #endif #if 1 double *wtmp; int *ijppt; double *mjpt, *prept, *curpt; int *mpjpt; #endif static TLS double mi = 0.0; static TLS double *m = NULL; static TLS int **ijp = NULL; static TLS int mpi = 0; static TLS int *mp = NULL; static TLS double *w1 = NULL; static TLS double *w2 = NULL; static TLS double *match = NULL; static TLS double *initverticalw = NULL; /* kufuu sureba iranai */ static TLS double *lastverticalw = NULL; /* kufuu sureba iranai */ static TLS char **mseq1 = NULL; static TLS char **mseq2 = NULL; static TLS char **mseq = NULL; static TLS int **intwork = NULL; static TLS double **doublework = NULL; static TLS int orlgth1 = 0, orlgth2 = 0; static TLS double **amino_dynamicmtx = NULL; // ?? int *warpis = NULL; int *warpjs = NULL; int *warpi = NULL; int *warpj = NULL; int *prevwarpi = NULL; int *prevwarpj = NULL; double *wmrecords = NULL; double *prevwmrecords = NULL; int warpn = 0; int warpbase; double curm = 0.0; double *wmrecordspt, *wmrecords1pt, *prevwmrecordspt; int *warpipt, *warpjpt; if( seq1 == NULL ) { if( orlgth1 > 0 && orlgth2 > 0 ) { orlgth1 = 0; orlgth2 = 0; if( mseq1 ) free( mseq1 ); mseq1 = NULL; if( mseq2 ) free( mseq2 ); mseq2 = NULL; if( w1 ) FreeFloatVec( w1 ); w1 = NULL; if( w2 ) FreeFloatVec( w2 ); w2 = NULL; if( match ) FreeFloatVec( match ); match = NULL; if( initverticalw ) FreeFloatVec( initverticalw ); initverticalw = NULL; if( lastverticalw ) FreeFloatVec( lastverticalw ); lastverticalw = NULL; if( m ) FreeFloatVec( m ); m = NULL; if( mp ) FreeIntVec( mp ); mp = NULL; if( mseq ) FreeCharMtx( mseq ); mseq = NULL; if( doublework ) FreeFloatMtx( doublework ); doublework = NULL; if( intwork ) FreeIntMtx( intwork ); intwork = NULL; if( amino_dynamicmtx ) FreeDoubleMtx( amino_dynamicmtx ); amino_dynamicmtx = NULL; } orlgth1 = 0; orlgth2 = 0; return( 0.0 ); } lgth1 = strlen( seq1[0] ); lgth2 = strlen( seq2[0] ); warpbase = lgth1 + lgth2; warpis = NULL; warpjs = NULL; warpn = 0; if( trywarp ) { // fprintf( stderr, "IN G__align11\n" ); if( headgp == 0 || tailgp == 0 ) { fprintf( stderr, "At present, headgp and tailgp must be 1.\n" ); exit( 1 ); } wmrecords = AllocateFloatVec( lgth2+1 ); warpi = AllocateIntVec( lgth2+1 ); warpj = AllocateIntVec( lgth2+1 ); prevwmrecords = AllocateFloatVec( lgth2+1 ); prevwarpi = AllocateIntVec( lgth2+1 ); prevwarpj = AllocateIntVec( lgth2+1 ); for( i=0; i orlgth1 || lgth2 > orlgth2 ) { int ll1, ll2; if( orlgth1 > 0 && orlgth2 > 0 ) { FreeFloatVec( w1 ); FreeFloatVec( w2 ); FreeFloatVec( match ); FreeFloatVec( initverticalw ); FreeFloatVec( lastverticalw ); FreeFloatVec( m ); FreeIntVec( mp ); FreeCharMtx( mseq ); FreeFloatMtx( doublework ); FreeIntMtx( intwork ); FreeDoubleMtx( amino_dynamicmtx ); } ll1 = MAX( (int)(1.3*lgth1), orlgth1 ) + 100; ll2 = MAX( (int)(1.3*lgth2), orlgth2 ) + 100; #if DEBUG fprintf( stderr, "\ntrying to allocate (%d+%d)xn matrices ... ", ll1, ll2 ); #endif w1 = AllocateFloatVec( ll2+2 ); w2 = AllocateFloatVec( ll2+2 ); match = AllocateFloatVec( ll2+2 ); initverticalw = AllocateFloatVec( ll1+2 ); lastverticalw = AllocateFloatVec( ll1+2 ); m = AllocateFloatVec( ll2+2 ); mp = AllocateIntVec( ll2+2 ); mseq = AllocateCharMtx( 2, ll1+ll2 ); // 2020/Apr doublework = AllocateFloatMtx( nalphabets, MAX( ll1, ll2 )+2 ); intwork = AllocateIntMtx( nalphabets, MAX( ll1, ll2 )+2 ); amino_dynamicmtx = AllocateDoubleMtx( 0x100, 0x100 ); #if DEBUG fprintf( stderr, "succeeded\n" ); #endif orlgth1 = ll1 - 100; orlgth2 = ll2 - 100; } for( i=0; i commonAlloc1 || orlgth2 > commonAlloc2 ) { int ll1, ll2; if( commonAlloc1 && commonAlloc2 ) { FreeIntMtx( commonIP ); } ll1 = MAX( orlgth1, commonAlloc1 ); ll2 = MAX( orlgth2, commonAlloc2 ); #if DEBUG fprintf( stderr, "\n\ntrying to allocate %dx%d matrices ... ", ll1+1, ll2+1 ); #endif commonIP = AllocateIntMtx( ll1+10, ll2+10 ); #if DEBUG fprintf( stderr, "succeeded\n\n" ); #endif commonAlloc1 = ll1; commonAlloc2 = ll2; } ijp = commonIP; #if 0 for( i=0; i", wm ); #endif #if 0 fprintf( stderr, "%5.0f?", g ); #endif if( (g=mi+fpenalty) > wm ) { wm = g; *ijppt = -( j - mpi ); } if( (g=*prept) >= mi ) // if( (g=*prept) > mi ) { mi = g; mpi = j-1; } #if USE_PENALTY_EX mi += fpenalty_ex_i; // mi += fpenalty_ex; #endif #if 0 fprintf( stderr, "%5.0f?", g ); #endif if( (g=*mjpt + fpenalty) > wm ) { wm = g; *ijppt = +( i - *mpjpt ); } if( (g=*prept) >= *mjpt ) // if( (g=*prept) > *mjpt ) { *mjpt = g; *mpjpt = i-1; } #if USE_PENALTY_EX if( j < lgth2 ) // 2018/May/11 m[j] += fpenalty_ex; #endif #if 1 if( trywarp ) { fpenalty_tmp = fpenalty_shift + fpenalty_ex * ( i - prevwarpi[j-1] + j - prevwarpj[j-1] ); // fprintf( stderr, "fpenalty_shift = %f\n", fpenalty_tmp ); // fprintf( stderr, "\n\n\nwarp to %c-%c (%d-%d) from %c-%c (%d-%d) ? prevwmrecords[%d] = %f + %f <- wm = %f\n", seq1[0][prevwarpi[j-1]], seq2[0][prevwarpj[j-1]], prevwarpi[j-1], prevwarpj[j-1], seq1[0][i], seq2[0][j], i, j, j, prevwmrecords[j-1], fpenalty_tmp, wm ); // if( (g=prevwmrecords[j-1] + fpenalty_shift )> wm ) if( ( g=*prevwmrecordspt++ + fpenalty_tmp )> wm ) // naka ha osokute kamawanai { // fprintf( stderr, "Yes! Warp!! from %d-%d (%c-%c) to %d-%d (%c-%c) fpenalty_tmp = %f! warpn = %d\n", i, j, seq1[0][i], seq2[0][j-1], prevwarpi[j-1], prevwarpj[j-1],seq1[0][prevwarpi[j-1]], seq2[0][prevwarpj[j-1]], fpenalty_tmp, warpn ); if( warpn && prevwarpi[j-1] == warpis[warpn-1] && prevwarpj[j-1] == warpjs[warpn-1] ) { *ijppt = warpbase + warpn - 1; } else { *ijppt = warpbase + warpn; warpis = realloc( warpis, sizeof(int) * ( warpn+1 ) ); warpjs = realloc( warpjs, sizeof(int) * ( warpn+1 ) ); warpis[warpn] = prevwarpi[j-1]; warpjs[warpn] = prevwarpj[j-1]; warpn++; } wm = g; } else { } curm = *curpt + wm; // fprintf( stderr, "###### curm = %f at %c-%c, i=%d, j=%d\n", curm, seq1[0][i], seq2[0][j], i, j ); // fprintf( stderr, "copy from i, j-1? %f > %f?\n", wmrecords[j-1], curm ); // if( wmrecords[j-1] > wmrecords[j] ) if( *wmrecords1pt > *wmrecordspt ) { // fprintf( stderr, "yes\n" ); // wmrecords[j] = wmrecords[j-1]; *wmrecordspt = *wmrecords1pt; // warpi[j] = warpi[j-1]; // warpj[j] = warpj[j-1]; *warpipt = *(warpipt-1); *warpjpt = *(warpjpt-1); // fprintf( stderr, "warpi[j]=%d, warpj[j]=%d wmrecords[j] = %f\n", warpi[j], warpj[j], wmrecords[j] ); } // else // { // fprintf( stderr, "no\n" ); // } // fprintf( stderr, " curm = %f at %c-%c\n", curm, seq1[0][i], seq2[0][j] ); // fprintf( stderr, " wmrecords[%d] = %f\n", j, wmrecords[j] ); // fprintf( stderr, "replace?\n" ); // if( curm > wmrecords[j] ) if( curm > *wmrecordspt ) { // fprintf( stderr, "yes at %d-%d (%c-%c), replaced warp: warpi[j]=%d, warpj[j]=%d warpn=%d, wmrecords[j] = %f -> %f\n", i, j, seq1[0][i], seq2[0][j], i, j, warpn, wmrecords[j], curm ); // wmrecords[j] = curm; *wmrecordspt = curm; // warpi[j] = i; // warpj[j] = j; *warpipt = i; *warpjpt = j; } // else // { // fprintf( stderr, "No! warpi[j]=%d, warpj[j]=%d wmrecords[j] = %f\n", warpi[j], warpj[j], wmrecords[j] ); // } // fprintf( stderr, "%d-%d (%c-%c) curm = %5.0f, wmrecords[j]=%f\n", i, j, seq1[0][i], seq2[0][j], curm, wmrecords[j] ); wmrecordspt++; wmrecords1pt++; warpipt++; warpjpt++; } #endif *curpt++ += wm; ijppt++; mjpt++; prept++; mpjpt++; } lastverticalw[i] = currentw[lgth2-1]; // lgth2==0 no toki error if( trywarp ) { fltncpy( prevwmrecords, wmrecords, lastj ); intncpy( prevwarpi, warpi, lastj ); intncpy( prevwarpj, warpj, lastj ); } } if( trywarp ) { // fprintf( stderr, "\nwm = %f\n", wm ); // fprintf( stderr, "warpn = %d\n", warpn ); free( wmrecords ); free( prevwmrecords ); free( warpi ); free( warpj ); free( prevwarpi ); free( prevwarpj ); } wmo = Atracking( currentw, lastverticalw, seq1, seq2, mseq1, mseq2, ijp, tailgp, warpis, warpjs, warpbase ); if( !tailgp ) wm = wmo; // reporterr( "wm (after tracking) = %f\n", wm ); if( warpis ) free( warpis ); if( warpjs ) free( warpjs ); resultlen = strlen( mseq1[0] ); if( alloclen < resultlen || resultlen > N ) { fprintf( stderr, "alloclen=%d, resultlen=%d, N=%d\n", alloclen, resultlen, N ); ErrorExit( "LENGTH OVER!\n" ); } strcpy( seq1[0], mseq1[0] ); strcpy( seq2[0], mseq2[0] ); #if 0 fprintf( stderr, "\n" ); fprintf( stderr, ">\n%s\n", mseq1[0] ); fprintf( stderr, ">\n%s\n", mseq2[0] ); fprintf( stderr, "wm = %f\n", wm ); #endif return( wm ); } double G__align11_noalign( double **n_dynamicmtx, int penal, int penal_ex, char **seq1, char **seq2, int alloclen ) /* warp mitaiou */ { // int k; register int i, j; int lasti; /* outgap == 0 -> lgth1, outgap == 1 -> lgth1+1 */ int lgth1, lgth2; // int resultlen; double wm; /* int ?????? */ double g; double *currentw, *previousw; double fpenalty = (double)penal; #if USE_PENALTY_EX double fpenalty_ex = (double)penal_ex; double fpenalty_ex_i; #endif #if 1 double *wtmp; double *mjpt, *prept, *curpt; // int *mpjpt; #endif static TLS double mi, *m; static TLS double *w1, *w2; static TLS double *match; static TLS double *initverticalw; /* kufuu sureba iranai */ static TLS double *lastverticalw; /* kufuu sureba iranai */ static TLS int **intwork; static TLS double **doublework; static TLS int orlgth1 = 0, orlgth2 = 0; static TLS double **amino_dynamicmtx; if( seq1 == NULL ) { if( orlgth1 > 0 && orlgth2 > 0 ) { orlgth1 = 0; orlgth2 = 0; FreeFloatVec( w1 ); FreeFloatVec( w2 ); FreeFloatVec( match ); FreeFloatVec( initverticalw ); FreeFloatVec( lastverticalw ); free( m ); FreeFloatMtx( doublework ); FreeIntMtx( intwork ); FreeDoubleMtx( amino_dynamicmtx ); } return( 0.0 ); } wm = 0.0; lgth1 = strlen( seq1[0] ); lgth2 = strlen( seq2[0] ); #if 0 if( lgth1 <= 0 || lgth2 <= 0 ) { fprintf( stderr, "WARNING (g11): lgth1=%d, lgth2=%d\n", lgth1, lgth2 ); } #endif if( lgth1 > orlgth1 || lgth2 > orlgth2 ) { int ll1, ll2; if( orlgth1 > 0 && orlgth2 > 0 ) { FreeFloatVec( w1 ); FreeFloatVec( w2 ); FreeFloatVec( match ); FreeFloatVec( initverticalw ); FreeFloatVec( lastverticalw ); FreeFloatVec( m ); FreeFloatMtx( doublework ); FreeIntMtx( intwork ); FreeDoubleMtx( amino_dynamicmtx ); } ll1 = MAX( (int)(1.3*lgth1), orlgth1 ) + 100; ll2 = MAX( (int)(1.3*lgth2), orlgth2 ) + 100; #if DEBUG fprintf( stderr, "\ntrying to allocate (%d+%d)xn matrices ... ", ll1, ll2 ); #endif w1 = AllocateFloatVec( ll2+2 ); w2 = AllocateFloatVec( ll2+2 ); match = AllocateFloatVec( ll2+2 ); initverticalw = AllocateFloatVec( ll1+2 ); lastverticalw = AllocateFloatVec( ll1+2 ); m = AllocateFloatVec( ll2+2 ); doublework = AllocateFloatMtx( nalphabets, MAX( ll1, ll2 )+2 ); intwork = AllocateIntMtx( nalphabets, MAX( ll1, ll2 )+2 ); // amino_dynamicmtx = AllocateDoubleMtx( 0x80, 0x80 ); amino_dynamicmtx = AllocateDoubleMtx( 0x100, 0x100 ); // 2017/Nov. constants.c no 'charsize' wo global hensuu nishita houga yoi? #if DEBUG fprintf( stderr, "succeeded\n" ); #endif orlgth1 = ll1 - 100; orlgth2 = ll2 - 100; } for( i=0; i", wm ); #endif #if 0 fprintf( stderr, "%5.0f?", g ); #endif if( (g=mi+fpenalty) > wm ) { wm = g; } // if( (g=*prept) >= mi ) if( (g=*prept) > mi ) // onaji hazu { mi = g; } #if USE_PENALTY_EX mi += fpenalty_ex_i; #endif #if 0 fprintf( stderr, "%5.0f?", g ); #endif if( (g=*mjpt + fpenalty) > wm ) { wm = g; } // if( (g=*prept) >= *mjpt ) if( (g=*prept) > *mjpt ) // onaji hazu { *mjpt = g; } #if USE_PENALTY_EX if( j < lgth2 ) // 2018/May/11 m[j] += fpenalty_ex; #endif #if 0 fprintf( stderr, "%5.0f ", wm ); #endif *curpt++ += wm; mjpt++; prept++; } lastverticalw[i] = currentw[lgth2-1]; // lgth2==0 no toki error } #if 0 fprintf( stderr, "\n" ); fprintf( stderr, ">\n%s\n", mseq1[0] ); fprintf( stderr, ">\n%s\n", mseq2[0] ); fprintf( stderr, "wm (noalign) = %f\n", wm ); #endif return( wm ); }