PRIMER_PRODUCT_SIZE_RANGE=75-20000 PRIMER_MAX_END_STABILITY=10 PRIMER_EXPLAIN_FLAG=1 PRIMER_MIN_SIZE=19 PRIMER_MAX_TM=75 PRIMER_NUM_RETURN=1 PRIMER_SEQUENCE_ID=test0 SEQUENCE=ATTGAGAATAGTCTTAGATGTGTCGAAGCAGGTACCCAAGCTGGCTTTTGTACTCTTCGGAGCTTCCTTGCTCAGTGA PRIMER_START_CODON_POSITION=-3 PRIMER_LEFT_EXPLAIN=considered 36, would not amplify any of the ORF 18, low tm 14, ok 4 PRIMER_RIGHT_EXPLAIN=considered 33, low tm 1, high end compl 1, high 3' stability 1, ok 30 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_STOP_CODON_POSITION=75 PRIMER_PAIR_PENALTY=69.7977 PRIMER_LEFT_PENALTY=68.113745 PRIMER_RIGHT_PENALTY=1.683953 PRIMER_LEFT_SEQUENCE=ATTGAGAATAGTCTTAGATGTGTCGAA PRIMER_RIGHT_SEQUENCE=ACTGAGCAAGGAAGCTCCG PRIMER_LEFT=0,27 PRIMER_RIGHT=75,19 PRIMER_LEFT_TM=58.886 PRIMER_RIGHT_TM=60.684 PRIMER_LEFT_GC_PERCENT=33.333 PRIMER_RIGHT_GC_PERCENT=57.895 PRIMER_LEFT_SELF_ANY=6.00 PRIMER_RIGHT_SELF_ANY=4.00 PRIMER_LEFT_SELF_END=2.00 PRIMER_RIGHT_SELF_END=2.00 PRIMER_LEFT_POSITION_PENALTY=60.000000 PRIMER_RIGHT_POSITION_PENALTY=0.000000 PRIMER_LEFT_END_STABILITY=8.7000 PRIMER_RIGHT_END_STABILITY=9.9000 PRIMER_PAIR_COMPL_ANY=3.00 PRIMER_PAIR_COMPL_END=2.00 PRIMER_PRODUCT_SIZE=76 = PRIMER_SEQUENCE_ID=test1 SEQUENCE=ATGGAGAATAGTCTTAGATGTGTCGAAGCAGGTACCCAAGCTGGCTTTTGTACTCTTCGGAGCTTCCTTGCTCAGTAA PRIMER_START_CODON_POSITION=0 PRIMER_LEFT_EXPLAIN=considered 36, would not amplify any of the ORF 18, low tm 13, high end compl 1, ok 4 PRIMER_RIGHT_EXPLAIN=considered 33, low tm 2, high 3' stability 1, ok 30 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_STOP_CODON_POSITION=75 PRIMER_PAIR_PENALTY=8.2055 PRIMER_LEFT_PENALTY=7.037233 PRIMER_RIGHT_PENALTY=1.168315 PRIMER_LEFT_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTCG PRIMER_RIGHT_SEQUENCE=TACTGAGCAAGGAAGCTCCG PRIMER_LEFT=0,25 PRIMER_RIGHT=76,20 PRIMER_LEFT_TM=57.963 PRIMER_RIGHT_TM=60.668 PRIMER_LEFT_GC_PERCENT=40.000 PRIMER_RIGHT_GC_PERCENT=55.000 PRIMER_LEFT_SELF_ANY=6.00 PRIMER_RIGHT_SELF_ANY=4.00 PRIMER_LEFT_SELF_END=2.00 PRIMER_RIGHT_SELF_END=2.00 PRIMER_LEFT_POSITION_PENALTY=0.000000 PRIMER_RIGHT_POSITION_PENALTY=0.500000 PRIMER_LEFT_END_STABILITY=8.4000 PRIMER_RIGHT_END_STABILITY=9.9000 PRIMER_PAIR_COMPL_ANY=4.00 PRIMER_PAIR_COMPL_END=2.00 PRIMER_PRODUCT_SIZE=77 = PRIMER_SEQUENCE_ID=test2 SEQUENCE=ATGGAGAATAGTCTTAGATGTGTCGAAGCAGGTACCCAAGCTGGCTTTTGTACTCTTCGGAGCTTCCTTGCTCAGTGA PRIMER_START_CODON_POSITION=0 PRIMER_LEFT_EXPLAIN=considered 36, would not amplify any of the ORF 18, low tm 13, high end compl 1, ok 4 PRIMER_RIGHT_EXPLAIN=considered 33, low tm 1, high end compl 1, high 3' stability 1, ok 30 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_STOP_CODON_POSITION=75 PRIMER_PAIR_PENALTY=8.7212 PRIMER_LEFT_PENALTY=7.037233 PRIMER_RIGHT_PENALTY=1.683953 PRIMER_LEFT_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTCG PRIMER_RIGHT_SEQUENCE=ACTGAGCAAGGAAGCTCCG PRIMER_LEFT=0,25 PRIMER_RIGHT=75,19 PRIMER_LEFT_TM=57.963 PRIMER_RIGHT_TM=60.684 PRIMER_LEFT_GC_PERCENT=40.000 PRIMER_RIGHT_GC_PERCENT=57.895 PRIMER_LEFT_SELF_ANY=6.00 PRIMER_RIGHT_SELF_ANY=4.00 PRIMER_LEFT_SELF_END=2.00 PRIMER_RIGHT_SELF_END=2.00 PRIMER_LEFT_POSITION_PENALTY=0.000000 PRIMER_RIGHT_POSITION_PENALTY=0.000000 PRIMER_LEFT_END_STABILITY=8.4000 PRIMER_RIGHT_END_STABILITY=9.9000 PRIMER_PAIR_COMPL_ANY=4.00 PRIMER_PAIR_COMPL_END=2.00 PRIMER_PRODUCT_SIZE=76 = PRIMER_SEQUENCE_ID=test3 SEQUENCE=ATGGAGAATAGTCTTAGATGTGTCGAAGCAGGTACCCAAGCTGGCTTTTGTACTCTTCGGAGCTTCCTTGCTCAGTGA PRIMER_START_CODON_POSITION=0 PRIMER_LEFT_EXPLAIN=considered 36, would not amplify any of the ORF 18, low tm 13, high end compl 1, ok 4 PRIMER_RIGHT_EXPLAIN=considered 33, low tm 1, high end compl 1, high 3' stability 1, ok 30 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_STOP_CODON_POSITION=75 PRIMER_PAIR_PENALTY=8.7212 PRIMER_LEFT_PENALTY=7.037233 PRIMER_RIGHT_PENALTY=1.683953 PRIMER_LEFT_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTCG PRIMER_RIGHT_SEQUENCE=ACTGAGCAAGGAAGCTCCG PRIMER_LEFT=0,25 PRIMER_RIGHT=75,19 PRIMER_LEFT_TM=57.963 PRIMER_RIGHT_TM=60.684 PRIMER_LEFT_GC_PERCENT=40.000 PRIMER_RIGHT_GC_PERCENT=57.895 PRIMER_LEFT_SELF_ANY=6.00 PRIMER_RIGHT_SELF_ANY=4.00 PRIMER_LEFT_SELF_END=2.00 PRIMER_RIGHT_SELF_END=2.00 PRIMER_LEFT_POSITION_PENALTY=0.000000 PRIMER_RIGHT_POSITION_PENALTY=0.000000 PRIMER_LEFT_END_STABILITY=8.4000 PRIMER_RIGHT_END_STABILITY=9.9000 PRIMER_PAIR_COMPL_ANY=4.00 PRIMER_PAIR_COMPL_END=2.00 PRIMER_PRODUCT_SIZE=76 = PRIMER_SEQUENCE_ID=test4 SEQUENCE=ATGGAGAATAGTCTTAGATGTGTCGAAGCAGGTACCCAAGCTGGCTTTTGTACTCTTCGGAGCTTCCTTGCTCAGATA PRIMER_START_CODON_POSITION=0 PRIMER_LEFT_EXPLAIN=considered 36, would not amplify any of the ORF 18, low tm 13, high end compl 1, ok 4 PRIMER_RIGHT_EXPLAIN=considered 33, low tm 1, high end compl 1, high 3' stability 1, ok 30 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_STOP_CODON_POSITION=-1 PRIMER_PAIR_PENALTY=9.9307 PRIMER_LEFT_PENALTY=7.037233 PRIMER_RIGHT_PENALTY=2.893427 PRIMER_LEFT_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTCG PRIMER_RIGHT_SEQUENCE=TATCTGAGCAAGGAAGCTCCG PRIMER_LEFT=0,25 PRIMER_RIGHT=77,21 PRIMER_LEFT_TM=57.963 PRIMER_RIGHT_TM=61.893 PRIMER_LEFT_GC_PERCENT=40.000 PRIMER_RIGHT_GC_PERCENT=52.381 PRIMER_LEFT_SELF_ANY=6.00 PRIMER_RIGHT_SELF_ANY=4.00 PRIMER_LEFT_SELF_END=2.00 PRIMER_RIGHT_SELF_END=2.00 PRIMER_LEFT_POSITION_PENALTY=0.000000 PRIMER_RIGHT_POSITION_PENALTY=0.000000 PRIMER_LEFT_END_STABILITY=8.4000 PRIMER_RIGHT_END_STABILITY=9.9000 PRIMER_PAIR_COMPL_ANY=5.00 PRIMER_PAIR_COMPL_END=2.00 PRIMER_PRODUCT_SIZE=78 = PRIMER_SEQUENCE_ID=test5 SEQUENCE=ATTGAGAATAGTCTTAGATGTGTCGAAGCAGGTACCCAAGCTGGCTTTTGTACTCTTCGGAGCTTCCTTGCTCAGATA PRIMER_START_CODON_POSITION=0 PRIMER_ERROR=No start codon at SEQUENCE_START_CODON_POSITION = PRIMER_SEQUENCE_ID=test6 INCLUDED_REGION=0,299 SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTGGGTACCCAAGCTGGCTTTTGTACTCTTCGGAGCTTCCTTGATGAGCGCGCATCTTCAAGTAACCGGTTTTCAAATGAAAGCTTTCACAGCACTGCGCTTCCTCTCAGAACCTTCTGATGCCGTCACAATGCGGGGAGGAAATGTCCTCCTCGACTGCTCCGCGGAGTCCGACCGAGGAGTTCCAGTGATCAAGTGGAAGAAAGATGGCATTCATCTGGCCTTGGGAATGGATGAAAGGAAGCAGCAACTTTCAAATGGGTCTCTGCTGATACAAATGATACTTCATTCCAGA PRIMER_START_CODON_POSITION=298 PRIMER_COMMENT=Should fail with "Start codon not contained in INCLUDED_REGION". PRIMER_ERROR=Start codon position not contained in SEQUENCE_INCLUDED_REGION = PRIMER_SEQUENCE_ID=test7 INCLUDED_REGION=0,300 SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTGGGTACCCAAGCTGGCTTTTGTACTCTTCGGAGCTTCCTTGATGAGCGCGCATCTTCAAGTAACCGGTTTTCAAATGAAAGCTTTCACAGCACTGCGCTTCCTCTCAGAACCTTCTGATGCCGTCACAATGCGGGGAGGAAATGTCCTCCTCGACTGCTCCGCGGAGTCCGACCGAGGAGTTCCAGTGATCAAGTGGAAGAAAGATGGCATTCATCTGGCCTTGGGAATGGATGAAAGGAAGCAGCAACTTTCAAATGGGTCTCTGCTGATAATGATGATACTTCATTCCATG PRIMER_START_CODON_POSITION=297 PRIMER_COMMENT=Should succeed. PRIMER_LEFT_EXPLAIN=considered 1864, would not amplify any of the ORF 1229, low tm 87, high tm 53, high 3' stability 25, ok 470 PRIMER_RIGHT_EXPLAIN=considered 1840, low tm 186, high tm 161, high 3' stability 25, ok 1468 PRIMER_PAIR_EXPLAIN=considered 1, ok 1 PRIMER_STOP_CODON_POSITION=-1 PRIMER_PAIR_PENALTY=2163.8303 PRIMER_LEFT_PENALTY=2160.043439 PRIMER_RIGHT_PENALTY=3.786842 PRIMER_LEFT_SEQUENCE=AAAGATGGCATTCATCTGGC PRIMER_RIGHT_SEQUENCE=CATTATCAGCAGAGACCCATTT PRIMER_LEFT=225,20 PRIMER_RIGHT=299,22 PRIMER_LEFT_TM=60.043 PRIMER_RIGHT_TM=58.213 PRIMER_LEFT_GC_PERCENT=45.000 PRIMER_RIGHT_GC_PERCENT=40.909 PRIMER_LEFT_SELF_ANY=6.00 PRIMER_RIGHT_SELF_ANY=4.00 PRIMER_LEFT_SELF_END=2.00 PRIMER_RIGHT_SELF_END=0.00 PRIMER_LEFT_POSITION_PENALTY=2160.000000 PRIMER_RIGHT_POSITION_PENALTY=0.000000 PRIMER_LEFT_END_STABILITY=9.7000 PRIMER_RIGHT_END_STABILITY=7.2000 PRIMER_PAIR_COMPL_ANY=4.00 PRIMER_PAIR_COMPL_END=2.00 PRIMER_PRODUCT_SIZE=75 = PRIMER_SEQUENCE_ID=test8 PRIMER_INSIDE_PENALTY=100.0 SEQUENCE=ATGGAGAATAGTCTTAGATGTGTCGAAGCAGGTACCCAAGCTGGCTTTTGTACTCTTCGGAGCTTCCTTGCTCAGATA PRIMER_START_CODON_POSITION=0 PRIMER_WARNING=Non-default inside penalty or outside penalty has no effect when number of targets is 0 PRIMER_ERROR=Cannot accept both SEQUENCE_START_CODON_POSITION and non-default arguments for PRIMER_INSIDE_PENALTY or PRIMER_OUTSIDE_PENALTY = PRIMER_SEQUENCE_ID=test9 PRIMER_INSIDE_PENALTY=-1.0 PRIMER_OUTSIDE_PENALTY=100.0 SEQUENCE=ATGGAGAATAGTCTTAGATGTGTCGAAGCAGGTACCCAAGCTGGCTTTTGTACTCTTCGGAGCTTCCTTGCTCAGATA PRIMER_START_CODON_POSITION=0 PRIMER_WARNING=Non-default inside penalty or outside penalty has no effect when number of targets is 0 PRIMER_ERROR=Cannot accept both SEQUENCE_START_CODON_POSITION and non-default arguments for PRIMER_INSIDE_PENALTY or PRIMER_OUTSIDE_PENALTY = PRIMER_PRODUCT_SIZE_RANGE=75-20000 PRIMER_OUTSIDE_PENALTY=0.0 PRIMER_MAX_END_STABILITY=10 PRIMER_EXPLAIN_FLAG=1 PRIMER_MIN_SIZE=19 PRIMER_MAX_TM=65 PRIMER_NUM_RETURN=20 PRIMER_SEQUENCE_ID=DCC/X76132 SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTGGGTACCCAAGCTGGCTTTTGTACTCTTCGGAGCTTCCTTGCTCAGCGCGCATCTTCAAGTAACCGGTTTTCAAATTAAAGCTTTCACAGCACTGCGCTTCCTCTCAGAACCTTCTGATGCCGTCACAATGCGGGGAGGAAATGTCCTCCTCGACTGCTCCGCGGAGTCCGACCGAGGAGTTCCAGTGATCAAGTGGAAGAAAGATGGCATTCATCTGGCCTTGGGAATGGATGAAAGGAAGCAGCAACTTTCAAATGGGTCTCTGCTGATACAAAACATACTTCATTCCAGACACCACAAGCCAGATGAGGGACTTTACCAATGTGAGGCATCTTTAGGAGATTCTGGCTCAATTATTAGTCGGACAGCAAAAGTTGCAGTAGCAGGACCACTGAGGTTCCTTTCACAGACAGAATCTGTCACAGCCTTCATGGGAGACACAGTGCTACTCAAGTGTGAAGTCATTGGGGAGCCCATGCCAACAATCCACTGGCAGAAGAACCAACAAGACCTGACTCCAATCCCAGGTGACTCCCGAGTGGTGGTCTTGCCCTCTGGAGCATTGCAGATCAGCCGACTCCAACCGGGGGACATTGGAATTTACCGATGCTCAGCTCGAAATCCAGCCAGCTCAAGAACAGGAAATGAAGCAGAAGTCAGAATTTTATCAGATCCAGGACTGCATAGACAGCTGTATTTTCTGCAAAGACCATCCAATGTAGTAGCCATTGAAGGAAAAGATGCTGTCCTGGAATGTTGTGTTTCTGGCTATCCTCCACCAAGTTTTACCTGGTTACGAGGCGAGGAAGTCATCCAACTCAGGTCTAAAAAGTATTCTTTATTGGGTGGAAGCAACTTGCTTATCTCCAATGTGACAGATGATGACAGTGGAATGTATACCTGTGTTGTCACATATAAAAATGAGAATATTAGTGCCTCTGCAGAGCTCACAGTCTTGGTTCCGCCATGGTTTTTAAATCATCCTTCCAACCTGTATGCCTATGAAAGCATGGATATTGAGTTTGAATGTACAGTCTCTGGAAAGCCTGTGCCCACTGTGAATTGGATGAAGAATGGAGATGTGGTCATTCCTAGTGATTATTTTCAGATAGTGGGAGGAAGCAACTTACGGATACTTGGGGTGGTGAAGTCAGATGAAGGCTTTTATCAATGTGTGGCTGAAAATGAGGCTGGAAATGCCCAGACCAGTGCACAGCTCATTGTCCCTAAGCCTGCAATCCCAAGCTCCAGTGTCCTCCCTTCGGCTCCCAGAGATGTGGTCCCTGTCTTGGTTTCCAGCCGATTTGTCCGTCTCAGCTGGCGCCCACCTGCAGAAGCGAAAGGGAACATTCAAACTTTCACGGTCTTTTTCTCCAGAGAAGGTGACAACAGGGAACGAGCATTGAATACAACACAGCCTGGGTCCCTTCAGCTCACTGTGGGAAACCTGAAGCCAGAAGCCATGTACACCTTTCGAGTTGTGGCTTACAATGAATGGGGACCGGGAGAGAGTTCTCAACCCATCAAGGTGGCCACACAGCCTGAGTTGCAAGTTCCAGGGCCAGTAGAAAACCTGCAAGCTGTATCTACCTCACCTACCTCAATTCTTATTACCTGGGAACCCCCTGCCTATGCAAACGGTCCAGTCCAAGGTTACAGATTGTTCTGCACTGAGGTGTCCACAGGAAAAGAACAGAATATAGAGGTTGATGGACTATCTTATAAACTGGAAGGCCTGAAAAAATTCACCGAATATAGTCTTCGATTCTTAGCTTATAATCGCTATGGTCCGGGCGTCTCTACTGATGATATAACAGTGGTTACACTTTCTGACGTGCCAAGTGCCCCGCCTCAGAACGTCTCCCTGGAAGTGGTCAATTCAAGAAGTATCAAAGTTAGCTGGCTGCCTCCTCCATCAGGAACACAAAATGGATTTATTACCGGCTATAAAATTCGACACAGAAAGACGACCCGCAGGGGTGAGATGGAAACACTGGAGCCAAACAACCTCTGGTACCTATTCACAGGACTGGAGAAAGGAAGTCAGTACAGTTTCCAGGTGTCAGCCATGACAGTCAATGGTACTGGACCACCTTCCAACTGGTATACTGCAGAGACTCCAGAGAATGATCTAGATGAATCTCAAGTTCCTGATCAACCAAGCTCTCTTCATGTGAGGCCCCAGACTAACTGCATCATCATGAGTTGGACTCCTCCCTTGAACCCAAACATCGTGGTGCGAGGTTATATTATCGGTTATGGCGTTGGGAGCCCTTACGCTGAGACAGTGCGTGTGGACAGCAAGCAGCGATATTATTCCATTGAGAGGTTAGAGTCAAGTTCCCATTATGTAATCTCCCTAAAAGCTTTTAACAATGCCGGAGAAGGAGTTCCTCTTTATGAAAGTGCCACCACCAGGTCTATAACCGATCCCACTGACCCAGTTGATTATTATCCTTTGCTTGATGATTTCCCCACCTCGGTCCCAGATCTCTCCACCCCCATGCTCCCACCAGTAGGTGTACAGGCTGTGGCTCTTACCCATGATGCTGTGAGGGTCAGCTGGGCAGACAACTCTGTCCCTAAGAACCAAAAGACGTCTGAGGTGCGACTTTACACCGTCCGGTGGAGAACCAGCTTTTCTGCAAGTGCAAAATACAAGTCAGAAGACACAACATCTCTAAGTTACACAGCAACAGGCCTCAAACCAAACACAATGTATGAATTCTCGGTCATGGTAACAAAAAACAGAAGGTCCAGTACTTGGAGCATGACTGCACATGCCACCACGTATGAAGCAGCCCCCACCTCTGCTCCCAAGGACTTTACAGTCATTACTAGGGAAGGGAAGCCTCGTGCCGTCATTGTGAGTTGGCAGCCTCCCTTGGAAGCCAATGGGAAAATTACTGCTTACATCTTATTTTATACCTTGGACAAGAACATCCCAATTGATGACTGGATTATGGAAACAATCAGTGGTGATAGGCTTACTCATCAAATCATGGATCTCAACCTTGATACTATGTATTACTTTCGAATTCAAGCACGAAATTCAAAAGGAGTGGGGCCACTCTCTGATCCCATCCTCTTCAGGACTCTGAAAGTGGAACACCCTGACAAAATGGCTAATGACCAAGGTCGTCATGGAGATGGAGGTTATTGGCCAGTTGATACTAATTTGATTGATAGAAGCACCCTAAATGAGCCGCCAATTGGACAAATGCACCCCCCGCATGGCAGTGTCACTCCTCAGAAGAACAGCAACCTGCTTGTGATCATTGTGGTCACCGTTGGTGTCATCACAGTGCTGGTAGTGGTCATCGTGGCTGTGATTTGCACCCGACGCTCTTCAGCCCAGCAGAGAAAGAAACGGGCCACCCACAGTGCTGGCAAAAGGAAGGGCAGCCAGAAGGACCTCCGACCCCCTGATCTTTGGATCCATCATGAAGAAATGGAGATGAAAAATATTGAAAAGCCATCTGGCACTGACCCTGCAGGAAGGGACTCTCCCATCCAAAGTTGCCAAGACCTCACACCAGTCAGCCACAGCCAGTCAGAAACCCAACTGGGAAGCAAAAGCACCTCTCATTCAGGTCAAGACACTGAGGAAGCAGGGAGCTCTATGTCCACTCTGGAGAGGTCGCTGGCTGCACGCCGAGCCCCCCGGGCCAAGCTCATGATTCCCATGGATGCCCAGTCCAACAATCCTGCTGTCGTGAGCGCCATCCCGGTGCCAACGCTAGAAAGTGCCCAGTACCCAGGAATCCTCCCGTCTCCCACCTGTGGATATCCCCACCCGCAGTTCACTCTCCGGCCTGTGCCATTCCCAACACTCTCAGTGGACCGAGGTTTCGGAGCAGGAAGAAGTCAGTCAGTGAGTGAAGGACCAACTACCCAACAACCACCTATGCTGCCCCCATCTCAGCCTGAGCATTCTAGCAGCGAGGAGGCACCAAGCAGAACCATCCCCACAGCTTGTGTTCGACCAACTCACCCACTCCGCAGCTTTGCTAATCCTTTGCTACCTCCACCAATGAGTGCAATAGAACCGAAAGTCCCTTACACACCACTTTTGTCTCAGCCAGGGCCCACTCTTCCTAAGACCCATGTGAAAACAGCCTCCCTTGGGTTGGCTGGAAAAGCAAGATCCCCTTTGCTTCCTGTGTCTGTGCCAACAGCCCCTGAAGTGTCTGAGGAGAGCCACAAACCAACAGAGGATTCAGCCAATGTGTATGAACAGGATGATCTGAGTGAACAAATGGCAAGTTTGGAAGGACTCATGAAGCAGCTTAATGCCATCACAGGCTCAGCCTTTTAACATGTATTTCTGAATGGATGAGGTGAATTTTCCGGGAACTTTGCAGCATACCAATTACCCATAAACAGCACACCTGTGTCCAAGAACTCTAACCAGTGTACAGGTCACCCATCAGGACCACTCAGTTAAGGAAGATCCTGAAGCAGTTCAGAAGGAATAAGCATTCCTTCTTTCACAGGCATCAGGAATTGTCAAATGATGATTATGAGTTCCCTAAACAAAAGCAAAGATGCATTTTCACTGCAATGTCAAAGTTTAGCTGCT PRIMER_START_CODON_POSITION=0 PRIMER_LEFT_EXPLAIN=considered 39774, would not amplify any of the ORF 27031, GC content failed 49, low tm 2568, high tm 5293, long poly-x seq 73, high 3' stability 94, ok 4666 PRIMER_RIGHT_EXPLAIN=considered 39257, GC content failed 148, low tm 8235, high tm 15926, high any compl 53, high end compl 11, long poly-x seq 76, high 3' stability 140, ok 14668 PRIMER_PAIR_EXPLAIN=considered 22, ok 22 PRIMER_STOP_CODON_POSITION=4341 PRIMER_PAIR_PENALTY=20.4222 PRIMER_LEFT_PENALTY=7.184184 PRIMER_RIGHT_PENALTY=13.237993 PRIMER_LEFT_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTGG PRIMER_RIGHT_SEQUENCE=TCAGAAATACATGTTAAAAGGCTGA PRIMER_LEFT=0,27 PRIMER_RIGHT=4356,25 PRIMER_LEFT_TM=60.184 PRIMER_RIGHT_TM=59.262 PRIMER_LEFT_GC_PERCENT=37.037 PRIMER_RIGHT_GC_PERCENT=32.000 PRIMER_LEFT_SELF_ANY=6.00 PRIMER_RIGHT_SELF_ANY=6.00 PRIMER_LEFT_SELF_END=0.00 PRIMER_RIGHT_SELF_END=1.00 PRIMER_LEFT_POSITION_PENALTY=0.000000 PRIMER_RIGHT_POSITION_PENALTY=7.500000 PRIMER_LEFT_END_STABILITY=8.8000 PRIMER_RIGHT_END_STABILITY=8.2000 PRIMER_PAIR_COMPL_ANY=6.00 PRIMER_PAIR_COMPL_END=1.00 PRIMER_PRODUCT_SIZE=4357 PRIMER_PAIR_PENALTY_1=20.5592 PRIMER_LEFT_1_PENALTY=7.184184 PRIMER_RIGHT_1_PENALTY=13.374972 PRIMER_LEFT_1_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTGG PRIMER_RIGHT_1_SEQUENCE=CAGAAATACATGTTAAAAGGCTGA PRIMER_LEFT_1=0,27 PRIMER_RIGHT_1=4355,24 PRIMER_LEFT_1_TM=60.184 PRIMER_RIGHT_1_TM=57.625 PRIMER_LEFT_1_GC_PERCENT=37.037 PRIMER_RIGHT_1_GC_PERCENT=33.333 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_LEFT_1_POSITION_PENALTY=0.000000 PRIMER_RIGHT_1_POSITION_PENALTY=7.000000 PRIMER_LEFT_1_END_STABILITY=8.8000 PRIMER_RIGHT_1_END_STABILITY=8.2000 PRIMER_PAIR_1_COMPL_ANY=6.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_1=4356 PRIMER_PAIR_PENALTY_2=20.6580 PRIMER_LEFT_2_PENALTY=7.184184 PRIMER_RIGHT_2_PENALTY=13.473771 PRIMER_LEFT_2_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTGG PRIMER_RIGHT_2_SEQUENCE=CAGAAATACATGTTAAAAGGCTGAG PRIMER_LEFT_2=0,27 PRIMER_RIGHT_2=4355,25 PRIMER_LEFT_2_TM=60.184 PRIMER_RIGHT_2_TM=58.526 PRIMER_LEFT_2_GC_PERCENT=37.037 PRIMER_RIGHT_2_GC_PERCENT=36.000 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_LEFT_2_POSITION_PENALTY=0.000000 PRIMER_RIGHT_2_POSITION_PENALTY=7.000000 PRIMER_LEFT_2_END_STABILITY=8.8000 PRIMER_RIGHT_2_END_STABILITY=6.7000 PRIMER_PAIR_2_COMPL_ANY=6.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_2=4356 PRIMER_PAIR_PENALTY_3=20.7611 PRIMER_LEFT_3_PENALTY=7.184184 PRIMER_RIGHT_3_PENALTY=13.576946 PRIMER_LEFT_3_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTGG PRIMER_RIGHT_3_SEQUENCE=TCAGAAATACATGTTAAAAGGCTGAG PRIMER_LEFT_3=0,27 PRIMER_RIGHT_3=4356,26 PRIMER_LEFT_3_TM=60.184 PRIMER_RIGHT_3_TM=60.077 PRIMER_LEFT_3_GC_PERCENT=37.037 PRIMER_RIGHT_3_GC_PERCENT=34.615 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=3.00 PRIMER_LEFT_3_POSITION_PENALTY=0.000000 PRIMER_RIGHT_3_POSITION_PENALTY=7.500000 PRIMER_LEFT_3_END_STABILITY=8.8000 PRIMER_RIGHT_3_END_STABILITY=6.7000 PRIMER_PAIR_3_COMPL_ANY=6.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_3=4357 PRIMER_PAIR_PENALTY_4=21.0592 PRIMER_LEFT_4_PENALTY=7.184184 PRIMER_RIGHT_4_PENALTY=13.874972 PRIMER_LEFT_4_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTGG PRIMER_RIGHT_4_SEQUENCE=TCAGAAATACATGTTAAAAGGCTG PRIMER_LEFT_4=0,27 PRIMER_RIGHT_4=4356,24 PRIMER_LEFT_4_TM=60.184 PRIMER_RIGHT_4_TM=57.625 PRIMER_LEFT_4_GC_PERCENT=37.037 PRIMER_RIGHT_4_GC_PERCENT=33.333 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=1.00 PRIMER_LEFT_4_POSITION_PENALTY=0.000000 PRIMER_RIGHT_4_POSITION_PENALTY=7.500000 PRIMER_LEFT_4_END_STABILITY=8.8000 PRIMER_RIGHT_4_END_STABILITY=9.7000 PRIMER_PAIR_4_COMPL_ANY=6.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_4=4357 PRIMER_PAIR_PENALTY_5=21.3353 PRIMER_LEFT_5_PENALTY=7.184184 PRIMER_RIGHT_5_PENALTY=14.151147 PRIMER_LEFT_5_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTGG PRIMER_RIGHT_5_SEQUENCE=TTCAGAAATACATGTTAAAAGGCTG PRIMER_LEFT_5=0,27 PRIMER_RIGHT_5=4357,25 PRIMER_LEFT_5_TM=60.184 PRIMER_RIGHT_5_TM=58.849 PRIMER_LEFT_5_GC_PERCENT=37.037 PRIMER_RIGHT_5_GC_PERCENT=32.000 PRIMER_LEFT_5_SELF_ANY=6.00 PRIMER_RIGHT_5_SELF_ANY=6.00 PRIMER_LEFT_5_SELF_END=0.00 PRIMER_RIGHT_5_SELF_END=1.00 PRIMER_LEFT_5_POSITION_PENALTY=0.000000 PRIMER_RIGHT_5_POSITION_PENALTY=8.000000 PRIMER_LEFT_5_END_STABILITY=8.8000 PRIMER_RIGHT_5_END_STABILITY=9.7000 PRIMER_PAIR_5_COMPL_ANY=6.00 PRIMER_PAIR_5_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_5=4358 PRIMER_PAIR_PENALTY_6=21.5665 PRIMER_LEFT_6_PENALTY=7.184184 PRIMER_RIGHT_6_PENALTY=14.382282 PRIMER_LEFT_6_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTGG PRIMER_RIGHT_6_SEQUENCE=TTCAGAAATACATGTTAAAAGGCTGA PRIMER_LEFT_6=0,27 PRIMER_RIGHT_6=4357,26 PRIMER_LEFT_6_TM=60.184 PRIMER_RIGHT_6_TM=60.382 PRIMER_LEFT_6_GC_PERCENT=37.037 PRIMER_RIGHT_6_GC_PERCENT=30.769 PRIMER_LEFT_6_SELF_ANY=6.00 PRIMER_RIGHT_6_SELF_ANY=6.00 PRIMER_LEFT_6_SELF_END=0.00 PRIMER_RIGHT_6_SELF_END=1.00 PRIMER_LEFT_6_POSITION_PENALTY=0.000000 PRIMER_RIGHT_6_POSITION_PENALTY=8.000000 PRIMER_LEFT_6_END_STABILITY=8.8000 PRIMER_RIGHT_6_END_STABILITY=8.2000 PRIMER_PAIR_6_COMPL_ANY=6.00 PRIMER_PAIR_6_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_6=4358 PRIMER_PAIR_PENALTY_7=22.0762 PRIMER_LEFT_7_PENALTY=8.838215 PRIMER_RIGHT_7_PENALTY=13.237993 PRIMER_LEFT_7_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTG PRIMER_RIGHT_7_SEQUENCE=TCAGAAATACATGTTAAAAGGCTGA PRIMER_LEFT_7=0,26 PRIMER_RIGHT_7=4356,25 PRIMER_LEFT_7_TM=57.162 PRIMER_RIGHT_7_TM=59.262 PRIMER_LEFT_7_GC_PERCENT=34.615 PRIMER_RIGHT_7_GC_PERCENT=32.000 PRIMER_LEFT_7_SELF_ANY=6.00 PRIMER_RIGHT_7_SELF_ANY=6.00 PRIMER_LEFT_7_SELF_END=0.00 PRIMER_RIGHT_7_SELF_END=1.00 PRIMER_LEFT_7_POSITION_PENALTY=0.000000 PRIMER_RIGHT_7_POSITION_PENALTY=7.500000 PRIMER_LEFT_7_END_STABILITY=7.0000 PRIMER_RIGHT_7_END_STABILITY=8.2000 PRIMER_PAIR_7_COMPL_ANY=6.00 PRIMER_PAIR_7_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_7=4357 PRIMER_PAIR_PENALTY_8=22.2132 PRIMER_LEFT_8_PENALTY=8.838215 PRIMER_RIGHT_8_PENALTY=13.374972 PRIMER_LEFT_8_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTG PRIMER_RIGHT_8_SEQUENCE=CAGAAATACATGTTAAAAGGCTGA PRIMER_LEFT_8=0,26 PRIMER_RIGHT_8=4355,24 PRIMER_LEFT_8_TM=57.162 PRIMER_RIGHT_8_TM=57.625 PRIMER_LEFT_8_GC_PERCENT=34.615 PRIMER_RIGHT_8_GC_PERCENT=33.333 PRIMER_LEFT_8_SELF_ANY=6.00 PRIMER_RIGHT_8_SELF_ANY=6.00 PRIMER_LEFT_8_SELF_END=0.00 PRIMER_RIGHT_8_SELF_END=1.00 PRIMER_LEFT_8_POSITION_PENALTY=0.000000 PRIMER_RIGHT_8_POSITION_PENALTY=7.000000 PRIMER_LEFT_8_END_STABILITY=7.0000 PRIMER_RIGHT_8_END_STABILITY=8.2000 PRIMER_PAIR_8_COMPL_ANY=6.00 PRIMER_PAIR_8_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_8=4356 PRIMER_PAIR_PENALTY_9=22.3120 PRIMER_LEFT_9_PENALTY=8.838215 PRIMER_RIGHT_9_PENALTY=13.473771 PRIMER_LEFT_9_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTG PRIMER_RIGHT_9_SEQUENCE=CAGAAATACATGTTAAAAGGCTGAG PRIMER_LEFT_9=0,26 PRIMER_RIGHT_9=4355,25 PRIMER_LEFT_9_TM=57.162 PRIMER_RIGHT_9_TM=58.526 PRIMER_LEFT_9_GC_PERCENT=34.615 PRIMER_RIGHT_9_GC_PERCENT=36.000 PRIMER_LEFT_9_SELF_ANY=6.00 PRIMER_RIGHT_9_SELF_ANY=6.00 PRIMER_LEFT_9_SELF_END=0.00 PRIMER_RIGHT_9_SELF_END=3.00 PRIMER_LEFT_9_POSITION_PENALTY=0.000000 PRIMER_RIGHT_9_POSITION_PENALTY=7.000000 PRIMER_LEFT_9_END_STABILITY=7.0000 PRIMER_RIGHT_9_END_STABILITY=6.7000 PRIMER_PAIR_9_COMPL_ANY=6.00 PRIMER_PAIR_9_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_9=4356 PRIMER_PAIR_PENALTY_10=22.4152 PRIMER_LEFT_10_PENALTY=8.838215 PRIMER_RIGHT_10_PENALTY=13.576946 PRIMER_LEFT_10_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTG PRIMER_RIGHT_10_SEQUENCE=TCAGAAATACATGTTAAAAGGCTGAG PRIMER_LEFT_10=0,26 PRIMER_RIGHT_10=4356,26 PRIMER_LEFT_10_TM=57.162 PRIMER_RIGHT_10_TM=60.077 PRIMER_LEFT_10_GC_PERCENT=34.615 PRIMER_RIGHT_10_GC_PERCENT=34.615 PRIMER_LEFT_10_SELF_ANY=6.00 PRIMER_RIGHT_10_SELF_ANY=6.00 PRIMER_LEFT_10_SELF_END=0.00 PRIMER_RIGHT_10_SELF_END=3.00 PRIMER_LEFT_10_POSITION_PENALTY=0.000000 PRIMER_RIGHT_10_POSITION_PENALTY=7.500000 PRIMER_LEFT_10_END_STABILITY=7.0000 PRIMER_RIGHT_10_END_STABILITY=6.7000 PRIMER_PAIR_10_COMPL_ANY=6.00 PRIMER_PAIR_10_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_10=4357 PRIMER_PAIR_PENALTY_11=22.5640 PRIMER_LEFT_11_PENALTY=7.184184 PRIMER_RIGHT_11_PENALTY=15.379819 PRIMER_LEFT_11_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTGG PRIMER_RIGHT_11_SEQUENCE=ATTCAGAAATACATGTTAAAAGGCTG PRIMER_LEFT_11=0,27 PRIMER_RIGHT_11=4358,26 PRIMER_LEFT_11_TM=60.184 PRIMER_RIGHT_11_TM=59.120 PRIMER_LEFT_11_GC_PERCENT=37.037 PRIMER_RIGHT_11_GC_PERCENT=30.769 PRIMER_LEFT_11_SELF_ANY=6.00 PRIMER_RIGHT_11_SELF_ANY=6.00 PRIMER_LEFT_11_SELF_END=0.00 PRIMER_RIGHT_11_SELF_END=1.00 PRIMER_LEFT_11_POSITION_PENALTY=0.000000 PRIMER_RIGHT_11_POSITION_PENALTY=8.500000 PRIMER_LEFT_11_END_STABILITY=8.8000 PRIMER_RIGHT_11_END_STABILITY=9.7000 PRIMER_PAIR_11_COMPL_ANY=6.00 PRIMER_PAIR_11_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_11=4359 PRIMER_PAIR_PENALTY_12=22.7132 PRIMER_LEFT_12_PENALTY=8.838215 PRIMER_RIGHT_12_PENALTY=13.874972 PRIMER_LEFT_12_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTG PRIMER_RIGHT_12_SEQUENCE=TCAGAAATACATGTTAAAAGGCTG PRIMER_LEFT_12=0,26 PRIMER_RIGHT_12=4356,24 PRIMER_LEFT_12_TM=57.162 PRIMER_RIGHT_12_TM=57.625 PRIMER_LEFT_12_GC_PERCENT=34.615 PRIMER_RIGHT_12_GC_PERCENT=33.333 PRIMER_LEFT_12_SELF_ANY=6.00 PRIMER_RIGHT_12_SELF_ANY=6.00 PRIMER_LEFT_12_SELF_END=0.00 PRIMER_RIGHT_12_SELF_END=1.00 PRIMER_LEFT_12_POSITION_PENALTY=0.000000 PRIMER_RIGHT_12_POSITION_PENALTY=7.500000 PRIMER_LEFT_12_END_STABILITY=7.0000 PRIMER_RIGHT_12_END_STABILITY=9.7000 PRIMER_PAIR_12_COMPL_ANY=6.00 PRIMER_PAIR_12_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_12=4357 PRIMER_PAIR_PENALTY_13=22.8956 PRIMER_LEFT_13_PENALTY=7.184184 PRIMER_RIGHT_13_PENALTY=15.711440 PRIMER_LEFT_13_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTGG PRIMER_RIGHT_13_SEQUENCE=CATTCAGAAATACATGTTAAAAGGC PRIMER_LEFT_13=0,27 PRIMER_RIGHT_13=4359,25 PRIMER_LEFT_13_TM=60.184 PRIMER_RIGHT_13_TM=58.289 PRIMER_LEFT_13_GC_PERCENT=37.037 PRIMER_RIGHT_13_GC_PERCENT=32.000 PRIMER_LEFT_13_SELF_ANY=6.00 PRIMER_RIGHT_13_SELF_ANY=6.00 PRIMER_LEFT_13_SELF_END=0.00 PRIMER_RIGHT_13_SELF_END=2.00 PRIMER_LEFT_13_POSITION_PENALTY=0.000000 PRIMER_RIGHT_13_POSITION_PENALTY=9.000000 PRIMER_LEFT_13_END_STABILITY=8.8000 PRIMER_RIGHT_13_END_STABILITY=9.7000 PRIMER_PAIR_13_COMPL_ANY=6.00 PRIMER_PAIR_13_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_13=4360 PRIMER_PAIR_PENALTY_14=22.9894 PRIMER_LEFT_14_PENALTY=8.838215 PRIMER_RIGHT_14_PENALTY=14.151147 PRIMER_LEFT_14_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTG PRIMER_RIGHT_14_SEQUENCE=TTCAGAAATACATGTTAAAAGGCTG PRIMER_LEFT_14=0,26 PRIMER_RIGHT_14=4357,25 PRIMER_LEFT_14_TM=57.162 PRIMER_RIGHT_14_TM=58.849 PRIMER_LEFT_14_GC_PERCENT=34.615 PRIMER_RIGHT_14_GC_PERCENT=32.000 PRIMER_LEFT_14_SELF_ANY=6.00 PRIMER_RIGHT_14_SELF_ANY=6.00 PRIMER_LEFT_14_SELF_END=0.00 PRIMER_RIGHT_14_SELF_END=1.00 PRIMER_LEFT_14_POSITION_PENALTY=0.000000 PRIMER_RIGHT_14_POSITION_PENALTY=8.000000 PRIMER_LEFT_14_END_STABILITY=7.0000 PRIMER_RIGHT_14_END_STABILITY=9.7000 PRIMER_PAIR_14_COMPL_ANY=6.00 PRIMER_PAIR_14_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_14=4358 PRIMER_PAIR_PENALTY_15=23.0640 PRIMER_LEFT_15_PENALTY=7.184184 PRIMER_RIGHT_15_PENALTY=15.879819 PRIMER_LEFT_15_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTGG PRIMER_RIGHT_15_SEQUENCE=CATTCAGAAATACATGTTAAAAGGCT PRIMER_LEFT_15=0,27 PRIMER_RIGHT_15=4359,26 PRIMER_LEFT_15_TM=60.184 PRIMER_RIGHT_15_TM=59.120 PRIMER_LEFT_15_GC_PERCENT=37.037 PRIMER_RIGHT_15_GC_PERCENT=30.769 PRIMER_LEFT_15_SELF_ANY=6.00 PRIMER_RIGHT_15_SELF_ANY=6.00 PRIMER_LEFT_15_SELF_END=0.00 PRIMER_RIGHT_15_SELF_END=3.00 PRIMER_LEFT_15_POSITION_PENALTY=0.000000 PRIMER_RIGHT_15_POSITION_PENALTY=9.000000 PRIMER_LEFT_15_END_STABILITY=8.8000 PRIMER_RIGHT_15_END_STABILITY=9.4000 PRIMER_PAIR_15_COMPL_ANY=6.00 PRIMER_PAIR_15_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_15=4360 PRIMER_PAIR_PENALTY_16=23.2205 PRIMER_LEFT_16_PENALTY=8.838215 PRIMER_RIGHT_16_PENALTY=14.382282 PRIMER_LEFT_16_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTG PRIMER_RIGHT_16_SEQUENCE=TTCAGAAATACATGTTAAAAGGCTGA PRIMER_LEFT_16=0,26 PRIMER_RIGHT_16=4357,26 PRIMER_LEFT_16_TM=57.162 PRIMER_RIGHT_16_TM=60.382 PRIMER_LEFT_16_GC_PERCENT=34.615 PRIMER_RIGHT_16_GC_PERCENT=30.769 PRIMER_LEFT_16_SELF_ANY=6.00 PRIMER_RIGHT_16_SELF_ANY=6.00 PRIMER_LEFT_16_SELF_END=0.00 PRIMER_RIGHT_16_SELF_END=1.00 PRIMER_LEFT_16_POSITION_PENALTY=0.000000 PRIMER_RIGHT_16_POSITION_PENALTY=8.000000 PRIMER_LEFT_16_END_STABILITY=7.0000 PRIMER_RIGHT_16_END_STABILITY=8.2000 PRIMER_PAIR_16_COMPL_ANY=6.00 PRIMER_PAIR_16_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_16=4358 PRIMER_PAIR_PENALTY_17=23.2662 PRIMER_LEFT_17_PENALTY=7.184184 PRIMER_RIGHT_17_PENALTY=16.081991 PRIMER_LEFT_17_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTGG PRIMER_RIGHT_17_SEQUENCE=ATTCAGAAATACATGTTAAAAGGCTGA PRIMER_LEFT_17=0,27 PRIMER_RIGHT_17=4358,27 PRIMER_LEFT_17_TM=60.184 PRIMER_RIGHT_17_TM=60.582 PRIMER_LEFT_17_GC_PERCENT=37.037 PRIMER_RIGHT_17_GC_PERCENT=29.630 PRIMER_LEFT_17_SELF_ANY=6.00 PRIMER_RIGHT_17_SELF_ANY=6.00 PRIMER_LEFT_17_SELF_END=0.00 PRIMER_RIGHT_17_SELF_END=1.00 PRIMER_LEFT_17_POSITION_PENALTY=0.000000 PRIMER_RIGHT_17_POSITION_PENALTY=8.500000 PRIMER_LEFT_17_END_STABILITY=8.8000 PRIMER_RIGHT_17_END_STABILITY=8.2000 PRIMER_PAIR_17_COMPL_ANY=6.00 PRIMER_PAIR_17_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_17=4359 PRIMER_PAIR_PENALTY_18=23.3048 PRIMER_LEFT_18_PENALTY=7.184184 PRIMER_RIGHT_18_PENALTY=16.120652 PRIMER_LEFT_18_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTGG PRIMER_RIGHT_18_SEQUENCE=TTCAGAAATACATGTTAAAAGGCTGAG PRIMER_LEFT_18=0,27 PRIMER_RIGHT_18=4357,27 PRIMER_LEFT_18_TM=60.184 PRIMER_RIGHT_18_TM=61.121 PRIMER_LEFT_18_GC_PERCENT=37.037 PRIMER_RIGHT_18_GC_PERCENT=33.333 PRIMER_LEFT_18_SELF_ANY=6.00 PRIMER_RIGHT_18_SELF_ANY=6.00 PRIMER_LEFT_18_SELF_END=0.00 PRIMER_RIGHT_18_SELF_END=3.00 PRIMER_LEFT_18_POSITION_PENALTY=0.000000 PRIMER_RIGHT_18_POSITION_PENALTY=8.000000 PRIMER_LEFT_18_END_STABILITY=8.8000 PRIMER_RIGHT_18_END_STABILITY=6.7000 PRIMER_PAIR_18_COMPL_ANY=6.00 PRIMER_PAIR_18_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_18=4358 PRIMER_PAIR_PENALTY_19=23.8715 PRIMER_LEFT_19_PENALTY=7.184184 PRIMER_RIGHT_19_PENALTY=16.687284 PRIMER_LEFT_19_SEQUENCE=ATGGAGAATAGTCTTAGATGTGTTTGG PRIMER_RIGHT_19_SEQUENCE=CCATTCAGAAATACATGTTAAAAGGC PRIMER_LEFT_19=0,27 PRIMER_RIGHT_19=4360,26 PRIMER_LEFT_19_TM=60.184 PRIMER_RIGHT_19_TM=61.187 PRIMER_LEFT_19_GC_PERCENT=37.037 PRIMER_RIGHT_19_GC_PERCENT=34.615 PRIMER_LEFT_19_SELF_ANY=6.00 PRIMER_RIGHT_19_SELF_ANY=6.00 PRIMER_LEFT_19_SELF_END=0.00 PRIMER_RIGHT_19_SELF_END=2.00 PRIMER_LEFT_19_POSITION_PENALTY=0.000000 PRIMER_RIGHT_19_POSITION_PENALTY=9.500000 PRIMER_LEFT_19_END_STABILITY=8.8000 PRIMER_RIGHT_19_END_STABILITY=9.7000 PRIMER_PAIR_19_COMPL_ANY=6.00 PRIMER_PAIR_19_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_19=4361 = PRIMER_SEQUENCE_ID=LEU8/L-SELECTING/X17519 SEQUENCE=CTCCCTTTGGGCAAGGACCTGAGACCCTTGTGCTAAGTCAAGAGGCTCAATGGGCTGCAGAAGAACTAGAGAAGGACCAAGCAAAGCCATGATATTTCCATGGAAATGTCAGAGCACCCAGAGGGACTTATGGAACATCTTCAAGTTGTGGGGGTGGACAATGCTCTGTTGTGATTTCCTGGCACATCATGGAACCGACTGCTGGACTTACCATTATTCTGAAAAACCCATGAACTGGCAAAGGGCTAGAAGATTCTGCCGAGACAATTACACAGATTTAGTTGCCATACAAAACAAGGCGGAAATTGAGTATCTGGAGAAGACTCTGCCTTTCAGTCGTTCTTACTACTGGATAGGAATCCGGAAGATAGGAGGAATATGGACGTGGGTGGGAACCAACAAATCTCTCACTGAAGAAGCAGAGAACTGGGGAGATGGTGAGCCCAACAACAAGAAGAACAAGGAGGACTGCGTGGAGATCTATATCAAGAGAAACAAAGATGCAGGCAAATGGAACGATGACGCCTGCCACAAACTAAAGGCAGCCCTCTGTTACACAGCTTCTTGCCAGCCCTGGTCATGCAGTGGCCATGGAGAATGTGTAGAAATCATCAATAATTACACCTGCAACTGTGATGTGGGGTACTATGGGCCCCAGTGTCAGTTTGTGATTCAGTGTGAGCCTTTGGAGGCCCCAGAGCTGGGTACCATGGACTGTACTCACTCTTTGGGAAACTTCAGCTTCAGCTCACAGTGTGCCTTCAGCTGCTCTGAAGGAACAAACTTAACTGGGATTGAAGAAACCACCTGTGGACCATTTGGAAACTGGTCATCTCCAGAACCAACCTGTCAAGTGATTCAGTGTGAGCCTCTATCAGCACCAGATTTGGGGATCATGAACTGTAGCCATCCCCTGGCCAGCTTCAGCTTTACCTCTGCATGTACCTTCATCTGCTCAGAAGGAACTGAGTTAATTGGGAAGAAGAAAACCATTTGTGAATCATCTGGAATCTGGTCAAATCCTAGTCCAATATGTCAAAAATTGGACAAAAGTTTCTCAATGATTAAGGAGGGTGATTATAACCCCCTCTTCATTCCAGTGGCAGTCATGGTTACTGCATTCTCTGGGTTGGCATTTATCATTTGGCTGGCAAGGAGATTAAAAAAAGGCAAGAAATCCAAGAGAAGTATGAATGACCCATATTAAATCGCCCTTGGTGAAAGAAAATTCTTGGAATACTAAAAATCATGAGATCCTTTAAATCCTTCCATGAAACGTTTTGTGTGGTGGCACCTCCTACGTCAAACATGAAGTGTGTTTCCTTCAGTGCATCTGGGAAGATTTCTACCTGACCAACAGTTCCTTCAGCTTCCATTTCGCCCCTCATTTATCCCTCAACCCCCAGCCCACAGGTGTTTATACAGCTCAGCTTTTTGTCTTTTCTGAGGAGAAACAAATAAGACCATAAAGGGAAAGGATTCATGTGGAATATAAAGATGGCTGACTTTGCTCTTTCTTGACTCTTGTTTTCAGTTTCAATTCAGTGCTGTACTTGATGACAGACACTTCTAAATGAAGTGCAAATTTGATACATATGTGAATATGGACTCAGTTTTCTTGCAGATCAAATTTCACGTCGTCTTCTGTATACTGTGGAGGTACACTCTTATAGAAAGTTCAAAAAGTCTACGCTCTCCTTTCTTTCTAACTCCAGTGAAGTAATGGGGTCCTGCTCAAGTTGAAAGAGTCCTATTTGCACTGTAGCCTCGCCGTCTGTGAATTGGACCATCCTATTTAACTGGCTTCAGCCTCCCCACCTTCTTCAGCCACCTCTCTTTTTCAGTTGGCTGACTTCCACACCTAGCATCTCATGAGTGCCAAGCAAAAGGAGAGAAGAGAGAAATAGCCTGCGCTGTTTTTTAGTTTGGGGGTTTTGCTGTTTCCTTTTATGAGACCCATTCCTATTTCTTATAGTCAATGTTTCTTTTATCACGATATTATTAGTAAGAAAACATCACTGAAATGCTAGCTGCAAGTGACATCTCTTTGATGTCATATGGAAGAGTTAAAACAGGTGGAGAAATTCCTTGATTCACAATGAAATGCTCTCCTTTCCCCTGCCCCCAGACCTTTTATCCGACTTACCTAGATTCTACATATTCTTTAAATTTCATCTCAGGCCTCCCTCAACCCCACCACTTCTTTTATAACTAGTCCTTTACTAATCCAACCCATGATGAGCTCCTCTTCCTGGCTTCTTACTGAAAGGTTACCCTGTAACATGCAATTTTGCATTTGAATAAAGCCTGCTTTTTAAGTGTT PRIMER_START_CODON_POSITION=49 PRIMER_LEFT_EXPLAIN=considered 19864, would not amplify any of the ORF 16423, low tm 794, high tm 1127, high end compl 1, long poly-x seq 23, high 3' stability 37, ok 1459 PRIMER_RIGHT_EXPLAIN=considered 19472, GC content failed 127, low tm 6190, high tm 5041, high end compl 1, long poly-x seq 45, high 3' stability 67, ok 8001 PRIMER_PAIR_EXPLAIN=considered 24, high end compl 2, ok 22 PRIMER_STOP_CODON_POSITION=1204 PRIMER_PAIR_PENALTY=6.7884 PRIMER_LEFT_PENALTY=1.909478 PRIMER_RIGHT_PENALTY=4.878922 PRIMER_LEFT_SEQUENCE=ATGGGCTGCAGAAGAACTAGA PRIMER_RIGHT_SEQUENCE=GGGCGATTTAATATGGGTCA PRIMER_LEFT=49,21 PRIMER_RIGHT=1213,20 PRIMER_LEFT_TM=59.091 PRIMER_RIGHT_TM=59.621 PRIMER_LEFT_GC_PERCENT=47.619 PRIMER_RIGHT_GC_PERCENT=45.000 PRIMER_LEFT_SELF_ANY=6.00 PRIMER_RIGHT_SELF_ANY=5.00 PRIMER_LEFT_SELF_END=2.00 PRIMER_RIGHT_SELF_END=1.00 PRIMER_LEFT_POSITION_PENALTY=0.000000 PRIMER_RIGHT_POSITION_PENALTY=4.500000 PRIMER_LEFT_END_STABILITY=5.7000 PRIMER_RIGHT_END_STABILITY=7.9000 PRIMER_PAIR_COMPL_ANY=2.00 PRIMER_PAIR_COMPL_END=1.00 PRIMER_PRODUCT_SIZE=1165 PRIMER_PAIR_PENALTY_1=6.9188 PRIMER_LEFT_1_PENALTY=2.039879 PRIMER_RIGHT_1_PENALTY=4.878922 PRIMER_LEFT_1_SEQUENCE=ATGGGCTGCAGAAGAACTAGAG PRIMER_RIGHT_1_SEQUENCE=GGGCGATTTAATATGGGTCA PRIMER_LEFT_1=49,22 PRIMER_RIGHT_1=1213,20 PRIMER_LEFT_1_TM=60.040 PRIMER_RIGHT_1_TM=59.621 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_LEFT_1_SELF_ANY=6.00 PRIMER_RIGHT_1_SELF_ANY=5.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_LEFT_1_POSITION_PENALTY=0.000000 PRIMER_RIGHT_1_POSITION_PENALTY=4.500000 PRIMER_LEFT_1_END_STABILITY=5.7000 PRIMER_RIGHT_1_END_STABILITY=7.9000 PRIMER_PAIR_1_COMPL_ANY=2.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_1=1165 PRIMER_PAIR_PENALTY_2=7.5160 PRIMER_LEFT_2_PENALTY=1.909478 PRIMER_RIGHT_2_PENALTY=5.606474 PRIMER_LEFT_2_SEQUENCE=ATGGGCTGCAGAAGAACTAGA PRIMER_RIGHT_2_SEQUENCE=GGGCGATTTAATATGGGTCAT PRIMER_LEFT_2=49,21 PRIMER_RIGHT_2=1213,21 PRIMER_LEFT_2_TM=59.091 PRIMER_RIGHT_2_TM=59.894 PRIMER_LEFT_2_GC_PERCENT=47.619 PRIMER_RIGHT_2_GC_PERCENT=42.857 PRIMER_LEFT_2_SELF_ANY=6.00 PRIMER_RIGHT_2_SELF_ANY=5.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_LEFT_2_POSITION_PENALTY=0.000000 PRIMER_RIGHT_2_POSITION_PENALTY=4.500000 PRIMER_LEFT_2_END_STABILITY=5.7000 PRIMER_RIGHT_2_END_STABILITY=6.3000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_2=1165 PRIMER_PAIR_PENALTY_3=7.6464 PRIMER_LEFT_3_PENALTY=2.039879 PRIMER_RIGHT_3_PENALTY=5.606474 PRIMER_LEFT_3_SEQUENCE=ATGGGCTGCAGAAGAACTAGAG PRIMER_RIGHT_3_SEQUENCE=GGGCGATTTAATATGGGTCAT PRIMER_LEFT_3=49,22 PRIMER_RIGHT_3=1213,21 PRIMER_LEFT_3_TM=60.040 PRIMER_RIGHT_3_TM=59.894 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=42.857 PRIMER_LEFT_3_SELF_ANY=6.00 PRIMER_RIGHT_3_SELF_ANY=5.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=3.00 PRIMER_LEFT_3_POSITION_PENALTY=0.000000 PRIMER_RIGHT_3_POSITION_PENALTY=4.500000 PRIMER_LEFT_3_END_STABILITY=5.7000 PRIMER_RIGHT_3_END_STABILITY=6.3000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_3=1165 PRIMER_PAIR_PENALTY_4=8.3537 PRIMER_LEFT_4_PENALTY=1.909478 PRIMER_RIGHT_4_PENALTY=6.444219 PRIMER_LEFT_4_SEQUENCE=ATGGGCTGCAGAAGAACTAGA PRIMER_RIGHT_4_SEQUENCE=GGCGATTTAATATGGGTCATTC PRIMER_LEFT_4=49,21 PRIMER_RIGHT_4=1212,22 PRIMER_LEFT_4_TM=59.091 PRIMER_RIGHT_4_TM=59.556 PRIMER_LEFT_4_GC_PERCENT=47.619 PRIMER_RIGHT_4_GC_PERCENT=40.909 PRIMER_LEFT_4_SELF_ANY=6.00 PRIMER_RIGHT_4_SELF_ANY=5.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_LEFT_4_POSITION_PENALTY=0.000000 PRIMER_RIGHT_4_POSITION_PENALTY=4.000000 PRIMER_LEFT_4_END_STABILITY=5.7000 PRIMER_RIGHT_4_END_STABILITY=6.9000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=3.00 PRIMER_PRODUCT_SIZE_4=1164 PRIMER_PAIR_PENALTY_5=8.4316 PRIMER_LEFT_5_PENALTY=1.909478 PRIMER_RIGHT_5_PENALTY=6.522147 PRIMER_LEFT_5_SEQUENCE=ATGGGCTGCAGAAGAACTAGA PRIMER_RIGHT_5_SEQUENCE=AGGGCGATTTAATATGGGTCA PRIMER_LEFT_5=49,21 PRIMER_RIGHT_5=1214,21 PRIMER_LEFT_5_TM=59.091 PRIMER_RIGHT_5_TM=60.522 PRIMER_LEFT_5_GC_PERCENT=47.619 PRIMER_RIGHT_5_GC_PERCENT=42.857 PRIMER_LEFT_5_SELF_ANY=6.00 PRIMER_RIGHT_5_SELF_ANY=5.00 PRIMER_LEFT_5_SELF_END=2.00 PRIMER_RIGHT_5_SELF_END=1.00 PRIMER_LEFT_5_POSITION_PENALTY=0.000000 PRIMER_RIGHT_5_POSITION_PENALTY=5.000000 PRIMER_LEFT_5_END_STABILITY=5.7000 PRIMER_RIGHT_5_END_STABILITY=7.9000 PRIMER_PAIR_5_COMPL_ANY=2.00 PRIMER_PAIR_5_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_5=1166 PRIMER_PAIR_PENALTY_6=8.4841 PRIMER_LEFT_6_PENALTY=2.039879 PRIMER_RIGHT_6_PENALTY=6.444219 PRIMER_LEFT_6_SEQUENCE=ATGGGCTGCAGAAGAACTAGAG PRIMER_RIGHT_6_SEQUENCE=GGCGATTTAATATGGGTCATTC PRIMER_LEFT_6=49,22 PRIMER_RIGHT_6=1212,22 PRIMER_LEFT_6_TM=60.040 PRIMER_RIGHT_6_TM=59.556 PRIMER_LEFT_6_GC_PERCENT=50.000 PRIMER_RIGHT_6_GC_PERCENT=40.909 PRIMER_LEFT_6_SELF_ANY=6.00 PRIMER_RIGHT_6_SELF_ANY=5.00 PRIMER_LEFT_6_SELF_END=2.00 PRIMER_RIGHT_6_SELF_END=0.00 PRIMER_LEFT_6_POSITION_PENALTY=0.000000 PRIMER_RIGHT_6_POSITION_PENALTY=4.000000 PRIMER_LEFT_6_END_STABILITY=5.7000 PRIMER_RIGHT_6_END_STABILITY=6.9000 PRIMER_PAIR_6_COMPL_ANY=3.00 PRIMER_PAIR_6_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_6=1164 PRIMER_PAIR_PENALTY_7=8.5620 PRIMER_LEFT_7_PENALTY=2.039879 PRIMER_RIGHT_7_PENALTY=6.522147 PRIMER_LEFT_7_SEQUENCE=ATGGGCTGCAGAAGAACTAGAG PRIMER_RIGHT_7_SEQUENCE=AGGGCGATTTAATATGGGTCA PRIMER_LEFT_7=49,22 PRIMER_RIGHT_7=1214,21 PRIMER_LEFT_7_TM=60.040 PRIMER_RIGHT_7_TM=60.522 PRIMER_LEFT_7_GC_PERCENT=50.000 PRIMER_RIGHT_7_GC_PERCENT=42.857 PRIMER_LEFT_7_SELF_ANY=6.00 PRIMER_RIGHT_7_SELF_ANY=5.00 PRIMER_LEFT_7_SELF_END=2.00 PRIMER_RIGHT_7_SELF_END=1.00 PRIMER_LEFT_7_POSITION_PENALTY=0.000000 PRIMER_RIGHT_7_POSITION_PENALTY=5.000000 PRIMER_LEFT_7_END_STABILITY=5.7000 PRIMER_RIGHT_7_END_STABILITY=7.9000 PRIMER_PAIR_7_COMPL_ANY=2.00 PRIMER_PAIR_7_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_7=1166 PRIMER_PAIR_PENALTY_8=8.5798 PRIMER_LEFT_8_PENALTY=1.909478 PRIMER_RIGHT_8_PENALTY=6.670364 PRIMER_LEFT_8_SEQUENCE=ATGGGCTGCAGAAGAACTAGA PRIMER_RIGHT_8_SEQUENCE=GCGATTTAATATGGGTCATTCA PRIMER_LEFT_8=49,21 PRIMER_RIGHT_8=1211,22 PRIMER_LEFT_8_TM=59.091 PRIMER_RIGHT_8_TM=58.830 PRIMER_LEFT_8_GC_PERCENT=47.619 PRIMER_RIGHT_8_GC_PERCENT=36.364 PRIMER_LEFT_8_SELF_ANY=6.00 PRIMER_RIGHT_8_SELF_ANY=5.00 PRIMER_LEFT_8_SELF_END=2.00 PRIMER_RIGHT_8_SELF_END=1.00 PRIMER_LEFT_8_POSITION_PENALTY=0.000000 PRIMER_RIGHT_8_POSITION_PENALTY=3.500000 PRIMER_LEFT_8_END_STABILITY=5.7000 PRIMER_RIGHT_8_END_STABILITY=6.9000 PRIMER_PAIR_8_COMPL_ANY=3.00 PRIMER_PAIR_8_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_8=1163 PRIMER_PAIR_PENALTY_9=8.6317 PRIMER_LEFT_9_PENALTY=1.909478 PRIMER_RIGHT_9_PENALTY=6.722200 PRIMER_LEFT_9_SEQUENCE=ATGGGCTGCAGAAGAACTAGA PRIMER_RIGHT_9_SEQUENCE=CAAGGGCGATTTAATATGGG PRIMER_LEFT_9=49,21 PRIMER_RIGHT_9=1216,20 PRIMER_LEFT_9_TM=59.091 PRIMER_RIGHT_9_TM=59.278 PRIMER_LEFT_9_GC_PERCENT=47.619 PRIMER_RIGHT_9_GC_PERCENT=45.000 PRIMER_LEFT_9_SELF_ANY=6.00 PRIMER_RIGHT_9_SELF_ANY=5.00 PRIMER_LEFT_9_SELF_END=2.00 PRIMER_RIGHT_9_SELF_END=2.00 PRIMER_LEFT_9_POSITION_PENALTY=0.000000 PRIMER_RIGHT_9_POSITION_PENALTY=6.000000 PRIMER_LEFT_9_END_STABILITY=5.7000 PRIMER_RIGHT_9_END_STABILITY=9.6000 PRIMER_PAIR_9_COMPL_ANY=2.00 PRIMER_PAIR_9_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_9=1168 PRIMER_PAIR_PENALTY_10=8.7102 PRIMER_LEFT_10_PENALTY=2.039879 PRIMER_RIGHT_10_PENALTY=6.670364 PRIMER_LEFT_10_SEQUENCE=ATGGGCTGCAGAAGAACTAGAG PRIMER_RIGHT_10_SEQUENCE=GCGATTTAATATGGGTCATTCA PRIMER_LEFT_10=49,22 PRIMER_RIGHT_10=1211,22 PRIMER_LEFT_10_TM=60.040 PRIMER_RIGHT_10_TM=58.830 PRIMER_LEFT_10_GC_PERCENT=50.000 PRIMER_RIGHT_10_GC_PERCENT=36.364 PRIMER_LEFT_10_SELF_ANY=6.00 PRIMER_RIGHT_10_SELF_ANY=5.00 PRIMER_LEFT_10_SELF_END=2.00 PRIMER_RIGHT_10_SELF_END=1.00 PRIMER_LEFT_10_POSITION_PENALTY=0.000000 PRIMER_RIGHT_10_POSITION_PENALTY=3.500000 PRIMER_LEFT_10_END_STABILITY=5.7000 PRIMER_RIGHT_10_END_STABILITY=6.9000 PRIMER_PAIR_10_COMPL_ANY=3.00 PRIMER_PAIR_10_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_10=1163 PRIMER_PAIR_PENALTY_11=8.7621 PRIMER_LEFT_11_PENALTY=2.039879 PRIMER_RIGHT_11_PENALTY=6.722200 PRIMER_LEFT_11_SEQUENCE=ATGGGCTGCAGAAGAACTAGAG PRIMER_RIGHT_11_SEQUENCE=CAAGGGCGATTTAATATGGG PRIMER_LEFT_11=49,22 PRIMER_RIGHT_11=1216,20 PRIMER_LEFT_11_TM=60.040 PRIMER_RIGHT_11_TM=59.278 PRIMER_LEFT_11_GC_PERCENT=50.000 PRIMER_RIGHT_11_GC_PERCENT=45.000 PRIMER_LEFT_11_SELF_ANY=6.00 PRIMER_RIGHT_11_SELF_ANY=5.00 PRIMER_LEFT_11_SELF_END=2.00 PRIMER_RIGHT_11_SELF_END=2.00 PRIMER_LEFT_11_POSITION_PENALTY=0.000000 PRIMER_RIGHT_11_POSITION_PENALTY=6.000000 PRIMER_LEFT_11_END_STABILITY=5.7000 PRIMER_RIGHT_11_END_STABILITY=9.6000 PRIMER_PAIR_11_COMPL_ANY=2.00 PRIMER_PAIR_11_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_11=1168 PRIMER_PAIR_PENALTY_12=8.9584 PRIMER_LEFT_12_PENALTY=1.909478 PRIMER_RIGHT_12_PENALTY=7.048962 PRIMER_LEFT_12_SEQUENCE=ATGGGCTGCAGAAGAACTAGA PRIMER_RIGHT_12_SEQUENCE=CAAGGGCGATTTAATATGGGT PRIMER_LEFT_12=49,21 PRIMER_RIGHT_12=1216,21 PRIMER_LEFT_12_TM=59.091 PRIMER_RIGHT_12_TM=60.049 PRIMER_LEFT_12_GC_PERCENT=47.619 PRIMER_RIGHT_12_GC_PERCENT=42.857 PRIMER_LEFT_12_SELF_ANY=6.00 PRIMER_RIGHT_12_SELF_ANY=5.00 PRIMER_LEFT_12_SELF_END=2.00 PRIMER_RIGHT_12_SELF_END=0.00 PRIMER_LEFT_12_POSITION_PENALTY=0.000000 PRIMER_RIGHT_12_POSITION_PENALTY=6.000000 PRIMER_LEFT_12_END_STABILITY=5.7000 PRIMER_RIGHT_12_END_STABILITY=9.4000 PRIMER_PAIR_12_COMPL_ANY=2.00 PRIMER_PAIR_12_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_12=1168 PRIMER_PAIR_PENALTY_13=9.0139 PRIMER_LEFT_13_PENALTY=1.909478 PRIMER_RIGHT_13_PENALTY=7.104387 PRIMER_LEFT_13_SEQUENCE=ATGGGCTGCAGAAGAACTAGA PRIMER_RIGHT_13_SEQUENCE=AGGGCGATTTAATATGGGTC PRIMER_LEFT_13=49,21 PRIMER_RIGHT_13=1214,20 PRIMER_LEFT_13_TM=59.091 PRIMER_RIGHT_13_TM=57.896 PRIMER_LEFT_13_GC_PERCENT=47.619 PRIMER_RIGHT_13_GC_PERCENT=45.000 PRIMER_LEFT_13_SELF_ANY=6.00 PRIMER_RIGHT_13_SELF_ANY=5.00 PRIMER_LEFT_13_SELF_END=2.00 PRIMER_RIGHT_13_SELF_END=2.00 PRIMER_LEFT_13_POSITION_PENALTY=0.000000 PRIMER_RIGHT_13_POSITION_PENALTY=5.000000 PRIMER_LEFT_13_END_STABILITY=5.7000 PRIMER_RIGHT_13_END_STABILITY=9.1000 PRIMER_PAIR_13_COMPL_ANY=2.00 PRIMER_PAIR_13_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_13=1166 PRIMER_PAIR_PENALTY_14=9.0672 PRIMER_LEFT_14_PENALTY=1.909478 PRIMER_RIGHT_14_PENALTY=7.157689 PRIMER_LEFT_14_SEQUENCE=ATGGGCTGCAGAAGAACTAGA PRIMER_RIGHT_14_SEQUENCE=GGCGATTTAATATGGGTCATT PRIMER_LEFT_14=49,21 PRIMER_RIGHT_14=1212,21 PRIMER_LEFT_14_TM=59.091 PRIMER_RIGHT_14_TM=57.842 PRIMER_LEFT_14_GC_PERCENT=47.619 PRIMER_RIGHT_14_GC_PERCENT=38.095 PRIMER_LEFT_14_SELF_ANY=6.00 PRIMER_RIGHT_14_SELF_ANY=5.00 PRIMER_LEFT_14_SELF_END=2.00 PRIMER_RIGHT_14_SELF_END=1.00 PRIMER_LEFT_14_POSITION_PENALTY=0.000000 PRIMER_RIGHT_14_POSITION_PENALTY=4.000000 PRIMER_LEFT_14_END_STABILITY=5.7000 PRIMER_RIGHT_14_END_STABILITY=6.9000 PRIMER_PAIR_14_COMPL_ANY=3.00 PRIMER_PAIR_14_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_14=1164 PRIMER_PAIR_PENALTY_15=9.0888 PRIMER_LEFT_15_PENALTY=2.039879 PRIMER_RIGHT_15_PENALTY=7.048962 PRIMER_LEFT_15_SEQUENCE=ATGGGCTGCAGAAGAACTAGAG PRIMER_RIGHT_15_SEQUENCE=CAAGGGCGATTTAATATGGGT PRIMER_LEFT_15=49,22 PRIMER_RIGHT_15=1216,21 PRIMER_LEFT_15_TM=60.040 PRIMER_RIGHT_15_TM=60.049 PRIMER_LEFT_15_GC_PERCENT=50.000 PRIMER_RIGHT_15_GC_PERCENT=42.857 PRIMER_LEFT_15_SELF_ANY=6.00 PRIMER_RIGHT_15_SELF_ANY=5.00 PRIMER_LEFT_15_SELF_END=2.00 PRIMER_RIGHT_15_SELF_END=0.00 PRIMER_LEFT_15_POSITION_PENALTY=0.000000 PRIMER_RIGHT_15_POSITION_PENALTY=6.000000 PRIMER_LEFT_15_END_STABILITY=5.7000 PRIMER_RIGHT_15_END_STABILITY=9.4000 PRIMER_PAIR_15_COMPL_ANY=2.00 PRIMER_PAIR_15_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_15=1168 PRIMER_PAIR_PENALTY_16=9.1443 PRIMER_LEFT_16_PENALTY=2.039879 PRIMER_RIGHT_16_PENALTY=7.104387 PRIMER_LEFT_16_SEQUENCE=ATGGGCTGCAGAAGAACTAGAG PRIMER_RIGHT_16_SEQUENCE=AGGGCGATTTAATATGGGTC PRIMER_LEFT_16=49,22 PRIMER_RIGHT_16=1214,20 PRIMER_LEFT_16_TM=60.040 PRIMER_RIGHT_16_TM=57.896 PRIMER_LEFT_16_GC_PERCENT=50.000 PRIMER_RIGHT_16_GC_PERCENT=45.000 PRIMER_LEFT_16_SELF_ANY=6.00 PRIMER_RIGHT_16_SELF_ANY=5.00 PRIMER_LEFT_16_SELF_END=2.00 PRIMER_RIGHT_16_SELF_END=2.00 PRIMER_LEFT_16_POSITION_PENALTY=0.000000 PRIMER_RIGHT_16_POSITION_PENALTY=5.000000 PRIMER_LEFT_16_END_STABILITY=5.7000 PRIMER_RIGHT_16_END_STABILITY=9.1000 PRIMER_PAIR_16_COMPL_ANY=2.00 PRIMER_PAIR_16_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_16=1166 PRIMER_PAIR_PENALTY_17=9.1976 PRIMER_LEFT_17_PENALTY=2.039879 PRIMER_RIGHT_17_PENALTY=7.157689 PRIMER_LEFT_17_SEQUENCE=ATGGGCTGCAGAAGAACTAGAG PRIMER_RIGHT_17_SEQUENCE=GGCGATTTAATATGGGTCATT PRIMER_LEFT_17=49,22 PRIMER_RIGHT_17=1212,21 PRIMER_LEFT_17_TM=60.040 PRIMER_RIGHT_17_TM=57.842 PRIMER_LEFT_17_GC_PERCENT=50.000 PRIMER_RIGHT_17_GC_PERCENT=38.095 PRIMER_LEFT_17_SELF_ANY=6.00 PRIMER_RIGHT_17_SELF_ANY=5.00 PRIMER_LEFT_17_SELF_END=2.00 PRIMER_RIGHT_17_SELF_END=1.00 PRIMER_LEFT_17_POSITION_PENALTY=0.000000 PRIMER_RIGHT_17_POSITION_PENALTY=4.000000 PRIMER_LEFT_17_END_STABILITY=5.7000 PRIMER_RIGHT_17_END_STABILITY=6.9000 PRIMER_PAIR_17_COMPL_ANY=3.00 PRIMER_PAIR_17_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_17=1164 PRIMER_PAIR_PENALTY_18=9.2077 PRIMER_LEFT_18_PENALTY=1.909478 PRIMER_RIGHT_18_PENALTY=7.298213 PRIMER_LEFT_18_SEQUENCE=ATGGGCTGCAGAAGAACTAGA PRIMER_RIGHT_18_SEQUENCE=AAGGGCGATTTAATATGGGTC PRIMER_LEFT_18=49,21 PRIMER_RIGHT_18=1215,21 PRIMER_LEFT_18_TM=59.091 PRIMER_RIGHT_18_TM=59.202 PRIMER_LEFT_18_GC_PERCENT=47.619 PRIMER_RIGHT_18_GC_PERCENT=42.857 PRIMER_LEFT_18_SELF_ANY=6.00 PRIMER_RIGHT_18_SELF_ANY=5.00 PRIMER_LEFT_18_SELF_END=2.00 PRIMER_RIGHT_18_SELF_END=2.00 PRIMER_LEFT_18_POSITION_PENALTY=0.000000 PRIMER_RIGHT_18_POSITION_PENALTY=5.500000 PRIMER_LEFT_18_END_STABILITY=5.7000 PRIMER_RIGHT_18_END_STABILITY=9.1000 PRIMER_PAIR_18_COMPL_ANY=2.00 PRIMER_PAIR_18_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_18=1167 PRIMER_PAIR_PENALTY_19=9.2811 PRIMER_LEFT_19_PENALTY=1.909478 PRIMER_RIGHT_19_PENALTY=7.371606 PRIMER_LEFT_19_SEQUENCE=ATGGGCTGCAGAAGAACTAGA PRIMER_RIGHT_19_SEQUENCE=GCGATTTAATATGGGTCATTCAT PRIMER_LEFT_19=49,21 PRIMER_RIGHT_19=1211,23 PRIMER_LEFT_19_TM=59.091 PRIMER_RIGHT_19_TM=59.128 PRIMER_LEFT_19_GC_PERCENT=47.619 PRIMER_RIGHT_19_GC_PERCENT=34.783 PRIMER_LEFT_19_SELF_ANY=6.00 PRIMER_RIGHT_19_SELF_ANY=5.00 PRIMER_LEFT_19_SELF_END=2.00 PRIMER_RIGHT_19_SELF_END=3.00 PRIMER_LEFT_19_POSITION_PENALTY=0.000000 PRIMER_RIGHT_19_POSITION_PENALTY=3.500000 PRIMER_LEFT_19_END_STABILITY=5.7000 PRIMER_RIGHT_19_END_STABILITY=6.9000 PRIMER_PAIR_19_COMPL_ANY=3.00 PRIMER_PAIR_19_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_19=1163 = PRIMER_SEQUENCE_ID=L40146/UBC4 PRIMER_START_CODON_POSITION=0 SEQUENCE=ATGGCGCTGAAAAGAATCCACAAGGAATTGAATGATCTGGCACGGGACCCTCCAGCACAGTGTTCAGCAGGTCCTGTTGGAGATGATATGTTCCATTGGCAAGCTACAATAATGGGGCCAAATGACAGTCCCTATCAGGGTGGAGTATTTTTCTTGACAATTCATTTCCCAACAGATTACCCCTTCAAACCACCTAAGGTTGCATTTACAACAAGAATTTATCATCCAAATATTAACAGTAATGGCAGCATTTGTCTTGATATTCTACGATCACAGTGGTCTCCAGCACTAACTATTTCAAAAGTACTCTTGTCCATCTGTTCTCTGTTGTGTGATCCCAATCCAGATGATCCTTTAGTGCCTGAGATTGCTCGGATCTACCAAACAGATAGAGAAAAGTACAACAGAATAGCTCGGGAATGGACTCAGAAGTATGCGATGTAA PRIMER_LEFT_EXPLAIN=considered 3285, would not amplify any of the ORF 2182, GC content failed 11, low tm 330, high tm 255, high end compl 1, high 3' stability 7, ok 499 PRIMER_RIGHT_EXPLAIN=considered 3267, GC content failed 36, low tm 1201, high tm 523, high 3' stability 8, ok 1499 PRIMER_PAIR_EXPLAIN=considered 56, high end compl 34, ok 22 PRIMER_STOP_CODON_POSITION=441 PRIMER_PAIR_PENALTY=6.8208 PRIMER_LEFT_PENALTY=4.584153 PRIMER_RIGHT_PENALTY=2.236656 PRIMER_LEFT_SEQUENCE=ATGGCGCTGAAAAGAATCCAC PRIMER_RIGHT_SEQUENCE=ACATCGCATACTTCTGAGTCCA PRIMER_LEFT=0,21 PRIMER_RIGHT=441,22 PRIMER_LEFT_TM=63.584 PRIMER_RIGHT_TM=59.763 PRIMER_LEFT_GC_PERCENT=47.619 PRIMER_RIGHT_GC_PERCENT=45.455 PRIMER_LEFT_SELF_ANY=4.00 PRIMER_RIGHT_SELF_ANY=3.00 PRIMER_LEFT_SELF_END=0.00 PRIMER_RIGHT_SELF_END=1.00 PRIMER_LEFT_POSITION_PENALTY=0.000000 PRIMER_RIGHT_POSITION_PENALTY=0.000000 PRIMER_LEFT_END_STABILITY=7.9000 PRIMER_RIGHT_END_STABILITY=7.9000 PRIMER_PAIR_COMPL_ANY=5.00 PRIMER_PAIR_COMPL_END=3.00 PRIMER_PRODUCT_SIZE=442 PRIMER_PAIR_PENALTY_1=8.3015 PRIMER_LEFT_1_PENALTY=4.584153 PRIMER_RIGHT_1_PENALTY=3.717312 PRIMER_LEFT_1_SEQUENCE=ATGGCGCTGAAAAGAATCCAC PRIMER_RIGHT_1_SEQUENCE=TACATCGCATACTTCTGAGTCCA PRIMER_LEFT_1=0,21 PRIMER_RIGHT_1=442,23 PRIMER_LEFT_1_TM=63.584 PRIMER_RIGHT_1_TM=59.783 PRIMER_LEFT_1_GC_PERCENT=47.619 PRIMER_RIGHT_1_GC_PERCENT=43.478 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=3.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_LEFT_1_POSITION_PENALTY=0.000000 PRIMER_RIGHT_1_POSITION_PENALTY=0.500000 PRIMER_LEFT_1_END_STABILITY=7.9000 PRIMER_RIGHT_1_END_STABILITY=7.9000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PRODUCT_SIZE_1=443 PRIMER_PAIR_PENALTY_2=8.4940 PRIMER_LEFT_2_PENALTY=2.909495 PRIMER_RIGHT_2_PENALTY=5.584511 PRIMER_LEFT_2_SEQUENCE=ATGGCGCTGAAAAGAATCCA PRIMER_RIGHT_2_SEQUENCE=TTACATCGCATACTTCTGAGTCC PRIMER_LEFT_2=0,20 PRIMER_RIGHT_2=443,23 PRIMER_LEFT_2_TM=62.909 PRIMER_RIGHT_2_TM=58.415 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=43.478 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=3.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=1.00 PRIMER_LEFT_2_POSITION_PENALTY=0.000000 PRIMER_RIGHT_2_POSITION_PENALTY=1.000000 PRIMER_LEFT_2_END_STABILITY=8.1000 PRIMER_RIGHT_2_END_STABILITY=7.6000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=3.00 PRIMER_PRODUCT_SIZE_2=444 PRIMER_PAIR_PENALTY_3=10.1687 PRIMER_LEFT_3_PENALTY=4.584153 PRIMER_RIGHT_3_PENALTY=5.584511 PRIMER_LEFT_3_SEQUENCE=ATGGCGCTGAAAAGAATCCAC PRIMER_RIGHT_3_SEQUENCE=TTACATCGCATACTTCTGAGTCC PRIMER_LEFT_3=0,21 PRIMER_RIGHT_3=443,23 PRIMER_LEFT_3_TM=63.584 PRIMER_RIGHT_3_TM=58.415 PRIMER_LEFT_3_GC_PERCENT=47.619 PRIMER_RIGHT_3_GC_PERCENT=43.478 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=3.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=1.00 PRIMER_LEFT_3_POSITION_PENALTY=0.000000 PRIMER_RIGHT_3_POSITION_PENALTY=1.000000 PRIMER_LEFT_3_END_STABILITY=7.9000 PRIMER_RIGHT_3_END_STABILITY=7.6000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_3=444 PRIMER_PAIR_PENALTY_4=10.6050 PRIMER_LEFT_4_PENALTY=4.584153 PRIMER_RIGHT_4_PENALTY=6.020829 PRIMER_LEFT_4_SEQUENCE=ATGGCGCTGAAAAGAATCCAC PRIMER_RIGHT_4_SEQUENCE=TTACATCGCATACTTCTGAGTCCA PRIMER_LEFT_4=0,21 PRIMER_RIGHT_4=443,24 PRIMER_LEFT_4_TM=63.584 PRIMER_RIGHT_4_TM=61.021 PRIMER_LEFT_4_GC_PERCENT=47.619 PRIMER_RIGHT_4_GC_PERCENT=41.667 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=3.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=1.00 PRIMER_LEFT_4_POSITION_PENALTY=0.000000 PRIMER_RIGHT_4_POSITION_PENALTY=1.000000 PRIMER_LEFT_4_END_STABILITY=7.9000 PRIMER_RIGHT_4_END_STABILITY=7.9000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=3.00 PRIMER_PRODUCT_SIZE_4=444 PRIMER_PAIR_PENALTY_5=63.2969 PRIMER_LEFT_5_PENALTY=61.060283 PRIMER_RIGHT_5_PENALTY=2.236656 PRIMER_LEFT_5_SEQUENCE=GCGCTGAAAAGAATCCACA PRIMER_RIGHT_5_SEQUENCE=ACATCGCATACTTCTGAGTCCA PRIMER_LEFT_5=3,19 PRIMER_RIGHT_5=441,22 PRIMER_LEFT_5_TM=59.940 PRIMER_RIGHT_5_TM=59.763 PRIMER_LEFT_5_GC_PERCENT=47.368 PRIMER_RIGHT_5_GC_PERCENT=45.455 PRIMER_LEFT_5_SELF_ANY=4.00 PRIMER_RIGHT_5_SELF_ANY=3.00 PRIMER_LEFT_5_SELF_END=0.00 PRIMER_RIGHT_5_SELF_END=1.00 PRIMER_LEFT_5_POSITION_PENALTY=60.000000 PRIMER_RIGHT_5_POSITION_PENALTY=0.000000 PRIMER_LEFT_5_END_STABILITY=8.2000 PRIMER_RIGHT_5_END_STABILITY=7.9000 PRIMER_PAIR_5_COMPL_ANY=5.00 PRIMER_PAIR_5_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_5=439 PRIMER_PAIR_PENALTY_6=63.5215 PRIMER_LEFT_6_PENALTY=61.284892 PRIMER_RIGHT_6_PENALTY=2.236656 PRIMER_LEFT_6_SEQUENCE=GCGCTGAAAAGAATCCACAA PRIMER_RIGHT_6_SEQUENCE=ACATCGCATACTTCTGAGTCCA PRIMER_LEFT_6=3,20 PRIMER_RIGHT_6=441,22 PRIMER_LEFT_6_TM=61.285 PRIMER_RIGHT_6_TM=59.763 PRIMER_LEFT_6_GC_PERCENT=45.000 PRIMER_RIGHT_6_GC_PERCENT=45.455 PRIMER_LEFT_6_SELF_ANY=4.00 PRIMER_RIGHT_6_SELF_ANY=3.00 PRIMER_LEFT_6_SELF_END=0.00 PRIMER_RIGHT_6_SELF_END=1.00 PRIMER_LEFT_6_POSITION_PENALTY=60.000000 PRIMER_RIGHT_6_POSITION_PENALTY=0.000000 PRIMER_LEFT_6_END_STABILITY=7.0000 PRIMER_RIGHT_6_END_STABILITY=7.9000 PRIMER_PAIR_6_COMPL_ANY=5.00 PRIMER_PAIR_6_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_6=439 PRIMER_PAIR_PENALTY_7=64.0998 PRIMER_LEFT_7_PENALTY=61.060283 PRIMER_RIGHT_7_PENALTY=3.039531 PRIMER_LEFT_7_SEQUENCE=GCGCTGAAAAGAATCCACA PRIMER_RIGHT_7_SEQUENCE=ACATCGCATACTTCTGAGTCCAT PRIMER_LEFT_7=3,19 PRIMER_RIGHT_7=441,23 PRIMER_LEFT_7_TM=59.940 PRIMER_RIGHT_7_TM=60.040 PRIMER_LEFT_7_GC_PERCENT=47.368 PRIMER_RIGHT_7_GC_PERCENT=43.478 PRIMER_LEFT_7_SELF_ANY=4.00 PRIMER_RIGHT_7_SELF_ANY=3.00 PRIMER_LEFT_7_SELF_END=0.00 PRIMER_RIGHT_7_SELF_END=2.00 PRIMER_LEFT_7_POSITION_PENALTY=60.000000 PRIMER_RIGHT_7_POSITION_PENALTY=0.000000 PRIMER_LEFT_7_END_STABILITY=8.2000 PRIMER_RIGHT_7_END_STABILITY=8.1000 PRIMER_PAIR_7_COMPL_ANY=5.00 PRIMER_PAIR_7_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_7=439 PRIMER_PAIR_PENALTY_8=64.3244 PRIMER_LEFT_8_PENALTY=61.284892 PRIMER_RIGHT_8_PENALTY=3.039531 PRIMER_LEFT_8_SEQUENCE=GCGCTGAAAAGAATCCACAA PRIMER_RIGHT_8_SEQUENCE=ACATCGCATACTTCTGAGTCCAT PRIMER_LEFT_8=3,20 PRIMER_RIGHT_8=441,23 PRIMER_LEFT_8_TM=61.285 PRIMER_RIGHT_8_TM=60.040 PRIMER_LEFT_8_GC_PERCENT=45.000 PRIMER_RIGHT_8_GC_PERCENT=43.478 PRIMER_LEFT_8_SELF_ANY=4.00 PRIMER_RIGHT_8_SELF_ANY=3.00 PRIMER_LEFT_8_SELF_END=0.00 PRIMER_RIGHT_8_SELF_END=2.00 PRIMER_LEFT_8_POSITION_PENALTY=60.000000 PRIMER_RIGHT_8_POSITION_PENALTY=0.000000 PRIMER_LEFT_8_END_STABILITY=7.0000 PRIMER_RIGHT_8_END_STABILITY=8.1000 PRIMER_PAIR_8_COMPL_ANY=5.00 PRIMER_PAIR_8_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_8=439 PRIMER_PAIR_PENALTY_9=64.7776 PRIMER_LEFT_9_PENALTY=61.060283 PRIMER_RIGHT_9_PENALTY=3.717312 PRIMER_LEFT_9_SEQUENCE=GCGCTGAAAAGAATCCACA PRIMER_RIGHT_9_SEQUENCE=TACATCGCATACTTCTGAGTCCA PRIMER_LEFT_9=3,19 PRIMER_RIGHT_9=442,23 PRIMER_LEFT_9_TM=59.940 PRIMER_RIGHT_9_TM=59.783 PRIMER_LEFT_9_GC_PERCENT=47.368 PRIMER_RIGHT_9_GC_PERCENT=43.478 PRIMER_LEFT_9_SELF_ANY=4.00 PRIMER_RIGHT_9_SELF_ANY=3.00 PRIMER_LEFT_9_SELF_END=0.00 PRIMER_RIGHT_9_SELF_END=1.00 PRIMER_LEFT_9_POSITION_PENALTY=60.000000 PRIMER_RIGHT_9_POSITION_PENALTY=0.500000 PRIMER_LEFT_9_END_STABILITY=8.2000 PRIMER_RIGHT_9_END_STABILITY=7.9000 PRIMER_PAIR_9_COMPL_ANY=5.00 PRIMER_PAIR_9_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_9=440 PRIMER_PAIR_PENALTY_10=65.0022 PRIMER_LEFT_10_PENALTY=61.284892 PRIMER_RIGHT_10_PENALTY=3.717312 PRIMER_LEFT_10_SEQUENCE=GCGCTGAAAAGAATCCACAA PRIMER_RIGHT_10_SEQUENCE=TACATCGCATACTTCTGAGTCCA PRIMER_LEFT_10=3,20 PRIMER_RIGHT_10=442,23 PRIMER_LEFT_10_TM=61.285 PRIMER_RIGHT_10_TM=59.783 PRIMER_LEFT_10_GC_PERCENT=45.000 PRIMER_RIGHT_10_GC_PERCENT=43.478 PRIMER_LEFT_10_SELF_ANY=4.00 PRIMER_RIGHT_10_SELF_ANY=3.00 PRIMER_LEFT_10_SELF_END=0.00 PRIMER_RIGHT_10_SELF_END=1.00 PRIMER_LEFT_10_POSITION_PENALTY=60.000000 PRIMER_RIGHT_10_POSITION_PENALTY=0.500000 PRIMER_LEFT_10_END_STABILITY=7.0000 PRIMER_RIGHT_10_END_STABILITY=7.9000 PRIMER_PAIR_10_COMPL_ANY=5.00 PRIMER_PAIR_10_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_10=440 PRIMER_PAIR_PENALTY_11=65.3838 PRIMER_LEFT_11_PENALTY=63.147152 PRIMER_RIGHT_11_PENALTY=2.236656 PRIMER_LEFT_11_SEQUENCE=GCGCTGAAAAGAATCCACAAG PRIMER_RIGHT_11_SEQUENCE=ACATCGCATACTTCTGAGTCCA PRIMER_LEFT_11=3,21 PRIMER_RIGHT_11=441,22 PRIMER_LEFT_11_TM=62.147 PRIMER_RIGHT_11_TM=59.763 PRIMER_LEFT_11_GC_PERCENT=47.619 PRIMER_RIGHT_11_GC_PERCENT=45.455 PRIMER_LEFT_11_SELF_ANY=4.00 PRIMER_RIGHT_11_SELF_ANY=3.00 PRIMER_LEFT_11_SELF_END=0.00 PRIMER_RIGHT_11_SELF_END=1.00 PRIMER_LEFT_11_POSITION_PENALTY=60.000000 PRIMER_RIGHT_11_POSITION_PENALTY=0.000000 PRIMER_LEFT_11_END_STABILITY=6.7000 PRIMER_RIGHT_11_END_STABILITY=7.9000 PRIMER_PAIR_11_COMPL_ANY=5.00 PRIMER_PAIR_11_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_11=439 PRIMER_PAIR_PENALTY_12=65.6084 PRIMER_LEFT_12_PENALTY=61.060283 PRIMER_RIGHT_12_PENALTY=4.548131 PRIMER_LEFT_12_SEQUENCE=GCGCTGAAAAGAATCCACA PRIMER_RIGHT_12_SEQUENCE=TACATCGCATACTTCTGAGTCCAT PRIMER_LEFT_12=3,19 PRIMER_RIGHT_12=442,24 PRIMER_LEFT_12_TM=59.940 PRIMER_RIGHT_12_TM=60.048 PRIMER_LEFT_12_GC_PERCENT=47.368 PRIMER_RIGHT_12_GC_PERCENT=41.667 PRIMER_LEFT_12_SELF_ANY=4.00 PRIMER_RIGHT_12_SELF_ANY=3.00 PRIMER_LEFT_12_SELF_END=0.00 PRIMER_RIGHT_12_SELF_END=2.00 PRIMER_LEFT_12_POSITION_PENALTY=60.000000 PRIMER_RIGHT_12_POSITION_PENALTY=0.500000 PRIMER_LEFT_12_END_STABILITY=8.2000 PRIMER_RIGHT_12_END_STABILITY=8.1000 PRIMER_PAIR_12_COMPL_ANY=5.00 PRIMER_PAIR_12_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_12=440 PRIMER_PAIR_PENALTY_13=65.8330 PRIMER_LEFT_13_PENALTY=61.284892 PRIMER_RIGHT_13_PENALTY=4.548131 PRIMER_LEFT_13_SEQUENCE=GCGCTGAAAAGAATCCACAA PRIMER_RIGHT_13_SEQUENCE=TACATCGCATACTTCTGAGTCCAT PRIMER_LEFT_13=3,20 PRIMER_RIGHT_13=442,24 PRIMER_LEFT_13_TM=61.285 PRIMER_RIGHT_13_TM=60.048 PRIMER_LEFT_13_GC_PERCENT=45.000 PRIMER_RIGHT_13_GC_PERCENT=41.667 PRIMER_LEFT_13_SELF_ANY=4.00 PRIMER_RIGHT_13_SELF_ANY=3.00 PRIMER_LEFT_13_SELF_END=0.00 PRIMER_RIGHT_13_SELF_END=2.00 PRIMER_LEFT_13_POSITION_PENALTY=60.000000 PRIMER_RIGHT_13_POSITION_PENALTY=0.500000 PRIMER_LEFT_13_END_STABILITY=7.0000 PRIMER_RIGHT_13_END_STABILITY=8.1000 PRIMER_PAIR_13_COMPL_ANY=5.00 PRIMER_PAIR_13_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_13=440 PRIMER_PAIR_PENALTY_14=66.1867 PRIMER_LEFT_14_PENALTY=63.147152 PRIMER_RIGHT_14_PENALTY=3.039531 PRIMER_LEFT_14_SEQUENCE=GCGCTGAAAAGAATCCACAAG PRIMER_RIGHT_14_SEQUENCE=ACATCGCATACTTCTGAGTCCAT PRIMER_LEFT_14=3,21 PRIMER_RIGHT_14=441,23 PRIMER_LEFT_14_TM=62.147 PRIMER_RIGHT_14_TM=60.040 PRIMER_LEFT_14_GC_PERCENT=47.619 PRIMER_RIGHT_14_GC_PERCENT=43.478 PRIMER_LEFT_14_SELF_ANY=4.00 PRIMER_RIGHT_14_SELF_ANY=3.00 PRIMER_LEFT_14_SELF_END=0.00 PRIMER_RIGHT_14_SELF_END=2.00 PRIMER_LEFT_14_POSITION_PENALTY=60.000000 PRIMER_RIGHT_14_POSITION_PENALTY=0.000000 PRIMER_LEFT_14_END_STABILITY=6.7000 PRIMER_RIGHT_14_END_STABILITY=8.1000 PRIMER_PAIR_14_COMPL_ANY=5.00 PRIMER_PAIR_14_COMPL_END=3.00 PRIMER_PRODUCT_SIZE_14=439 PRIMER_PAIR_PENALTY_15=66.3073 PRIMER_LEFT_15_PENALTY=61.060283 PRIMER_RIGHT_15_PENALTY=5.247065 PRIMER_LEFT_15_SEQUENCE=GCGCTGAAAAGAATCCACA PRIMER_RIGHT_15_SEQUENCE=ACATCGCATACTTCTGAGTCCATT PRIMER_LEFT_15=3,19 PRIMER_RIGHT_15=441,24 PRIMER_LEFT_15_TM=59.940 PRIMER_RIGHT_15_TM=61.247 PRIMER_LEFT_15_GC_PERCENT=47.368 PRIMER_RIGHT_15_GC_PERCENT=41.667 PRIMER_LEFT_15_SELF_ANY=4.00 PRIMER_RIGHT_15_SELF_ANY=3.00 PRIMER_LEFT_15_SELF_END=0.00 PRIMER_RIGHT_15_SELF_END=1.00 PRIMER_LEFT_15_POSITION_PENALTY=60.000000 PRIMER_RIGHT_15_POSITION_PENALTY=0.000000 PRIMER_LEFT_15_END_STABILITY=8.2000 PRIMER_RIGHT_15_END_STABILITY=8.4000 PRIMER_PAIR_15_COMPL_ANY=5.00 PRIMER_PAIR_15_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_15=439 PRIMER_PAIR_PENALTY_16=66.5320 PRIMER_LEFT_16_PENALTY=61.284892 PRIMER_RIGHT_16_PENALTY=5.247065 PRIMER_LEFT_16_SEQUENCE=GCGCTGAAAAGAATCCACAA PRIMER_RIGHT_16_SEQUENCE=ACATCGCATACTTCTGAGTCCATT PRIMER_LEFT_16=3,20 PRIMER_RIGHT_16=441,24 PRIMER_LEFT_16_TM=61.285 PRIMER_RIGHT_16_TM=61.247 PRIMER_LEFT_16_GC_PERCENT=45.000 PRIMER_RIGHT_16_GC_PERCENT=41.667 PRIMER_LEFT_16_SELF_ANY=4.00 PRIMER_RIGHT_16_SELF_ANY=3.00 PRIMER_LEFT_16_SELF_END=0.00 PRIMER_RIGHT_16_SELF_END=1.00 PRIMER_LEFT_16_POSITION_PENALTY=60.000000 PRIMER_RIGHT_16_POSITION_PENALTY=0.000000 PRIMER_LEFT_16_END_STABILITY=7.0000 PRIMER_RIGHT_16_END_STABILITY=8.4000 PRIMER_PAIR_16_COMPL_ANY=5.00 PRIMER_PAIR_16_COMPL_END=3.00 PRIMER_PRODUCT_SIZE_16=439 PRIMER_PAIR_PENALTY_17=66.6448 PRIMER_LEFT_17_PENALTY=61.060283 PRIMER_RIGHT_17_PENALTY=5.584511 PRIMER_LEFT_17_SEQUENCE=GCGCTGAAAAGAATCCACA PRIMER_RIGHT_17_SEQUENCE=TTACATCGCATACTTCTGAGTCC PRIMER_LEFT_17=3,19 PRIMER_RIGHT_17=443,23 PRIMER_LEFT_17_TM=59.940 PRIMER_RIGHT_17_TM=58.415 PRIMER_LEFT_17_GC_PERCENT=47.368 PRIMER_RIGHT_17_GC_PERCENT=43.478 PRIMER_LEFT_17_SELF_ANY=4.00 PRIMER_RIGHT_17_SELF_ANY=3.00 PRIMER_LEFT_17_SELF_END=0.00 PRIMER_RIGHT_17_SELF_END=1.00 PRIMER_LEFT_17_POSITION_PENALTY=60.000000 PRIMER_RIGHT_17_POSITION_PENALTY=1.000000 PRIMER_LEFT_17_END_STABILITY=8.2000 PRIMER_RIGHT_17_END_STABILITY=7.6000 PRIMER_PAIR_17_COMPL_ANY=5.00 PRIMER_PAIR_17_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_17=441 PRIMER_PAIR_PENALTY_18=66.8645 PRIMER_LEFT_18_PENALTY=63.147152 PRIMER_RIGHT_18_PENALTY=3.717312 PRIMER_LEFT_18_SEQUENCE=GCGCTGAAAAGAATCCACAAG PRIMER_RIGHT_18_SEQUENCE=TACATCGCATACTTCTGAGTCCA PRIMER_LEFT_18=3,21 PRIMER_RIGHT_18=442,23 PRIMER_LEFT_18_TM=62.147 PRIMER_RIGHT_18_TM=59.783 PRIMER_LEFT_18_GC_PERCENT=47.619 PRIMER_RIGHT_18_GC_PERCENT=43.478 PRIMER_LEFT_18_SELF_ANY=4.00 PRIMER_RIGHT_18_SELF_ANY=3.00 PRIMER_LEFT_18_SELF_END=0.00 PRIMER_RIGHT_18_SELF_END=1.00 PRIMER_LEFT_18_POSITION_PENALTY=60.000000 PRIMER_RIGHT_18_POSITION_PENALTY=0.500000 PRIMER_LEFT_18_END_STABILITY=6.7000 PRIMER_RIGHT_18_END_STABILITY=7.9000 PRIMER_PAIR_18_COMPL_ANY=5.00 PRIMER_PAIR_18_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_18=440 PRIMER_PAIR_PENALTY_19=66.8694 PRIMER_LEFT_19_PENALTY=61.284892 PRIMER_RIGHT_19_PENALTY=5.584511 PRIMER_LEFT_19_SEQUENCE=GCGCTGAAAAGAATCCACAA PRIMER_RIGHT_19_SEQUENCE=TTACATCGCATACTTCTGAGTCC PRIMER_LEFT_19=3,20 PRIMER_RIGHT_19=443,23 PRIMER_LEFT_19_TM=61.285 PRIMER_RIGHT_19_TM=58.415 PRIMER_LEFT_19_GC_PERCENT=45.000 PRIMER_RIGHT_19_GC_PERCENT=43.478 PRIMER_LEFT_19_SELF_ANY=4.00 PRIMER_RIGHT_19_SELF_ANY=3.00 PRIMER_LEFT_19_SELF_END=0.00 PRIMER_RIGHT_19_SELF_END=1.00 PRIMER_LEFT_19_POSITION_PENALTY=60.000000 PRIMER_RIGHT_19_POSITION_PENALTY=1.000000 PRIMER_LEFT_19_END_STABILITY=7.0000 PRIMER_RIGHT_19_END_STABILITY=7.6000 PRIMER_PAIR_19_COMPL_ANY=5.00 PRIMER_PAIR_19_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_19=441 = PRIMER_SEQUENCE_ID=U51903/IQGAP2 PRIMER_START_CODON_POSITION=222 PRIMER_LEFT_INPUT=ATGCCACACGAAGAGCTGC SEQUENCE=GAGGGAGGAGAGTTCACTTTTACTTCAGTGTCAGCGCGCGGCGGCCGTGGCTGGCTCTGGCGAGAGAGCACCGAGGGAGTGGGTCGCAGATCTTCGGGCGGCTAGGGGAAATCGGCGAGAGGCGGGATCCGAGCGCGCCGGCGGGGCGCAGAGCCCGCGAGCCTGGCCAGCGAGGGTAGCCGCGGGGGGCGCGCCCCGGGCGGGCCCCCGGAGACGCGCAGGATGCCACACGAAGAGCTGCCGTCGCTGCAGAGACCCCGCTATGGCTCTATTGTGGACGATGAAAGGCTCTCTGCAGAGGAGATGGATGAGAGGAGGCGGCAGAACATTGCTTATGAATATCTGTGCCACTTAGAGGAAGCCAAAAGGTGGATGGAAGTTTGCTTAGTTGAAGAATTGCCACCAACCACTGAATTGGAAGAAGGGCTCCGGAATGGAGTTTACCTTGCAAAGTTAGCCAAGTTCTTTGCCCCGAAAATGGTATCAGAGAAAAAGATCTATGATGTGGAACAAACACGTTATAAGAAGTCTGGCCTTCATTTTCGACACACAGATAATACCGTCCAGTGGTTAAGAGCGATGGAGTCTATTGGTCTACCCAAGATATTTTATCCAGAAACAACAGATGTCTATGATCGGAAAAACATACCAAGAATGATATATTGCATTCACGCACTGAGTTTGTATCTGTTCAAACTAGGAATAGCACCCCAGATCCAGGATTTGTTGGGCAAAGTAGACTTCACAGAGGAGGAAATCAGTAATATGAGAAAAGAACTTGAGAAATATGGAATACAGATGCCATCTTTCAGCAAAATAGGTGGTATTCTGGCCAATGAACTGTCCGTGGATGAAGCTGCATTACATGCTGCAGTTATAGCCATTAATGAAGCAGTTGAAAAAGGAATAGCAGAGCAAACCGTTGTAACACTAAGAAACCCAAATGCGGTTTTAACTTTAGTGGATGACAACCTTGCACCAGAATATCAGAAAGAACTCTGGGATGCCAAAAAGAAAAAAGAGGAAAATGCAAGACTGAAGAATAGCTGTATTTCAGAAGAAGAAAGAGATGCTTATGAAGAACTGCTGACACAAGCAGAAATCCAAGGCAATATTAATAAAGTCAACAGGCAGGCTGCAGTGGACCATATCAATGCTGTCATTCCGGAAGGTGACCCCGAGAATACGCTGCTTGCACTGAAGAAACCAGAGGCCCAGCTGCCTGCTGTTTATCCCTTTGCTGCTGCCATGTATCAGAACGAACTTTTCAACCTCCAGAAACAGAACACCATGAACTACTTGGCCCACGAGGAGCTTTTGATTGCTGTGGAAATGTTGTCTGCTGTTGCTTTACTAAACCAGGCCTTGGAAAGCAACGATCTTGTGTCTGTGCAGAATCAACTCAGAAGCCCCGCAATAGGCTTAAACAATCTGGACAAGGCATATGTGGAACGTTATGCAAACACACTACTCTCTGTTAAACTAGAAGTTTTATCCCAAGGGCAAGATAACTTAAGCTGGAATGAAATTCAGAATTGTATTGATATGGTTAATGCTCAAATTCAAGAAGAAAATGACCGAGTTGTAGCTGTAGGGTACATCAATGAAGCTATTGATGAAGGGAATCCTTTGAGGACTTTAGAAACTTTGCTCCTACCTACTGCGAATATTAGTGATGTGGACCCAGCCCATGCCCAGCACTACCAGGATGTTTTATACCATGCTAAATCACAGAAACTCGGAGACTCTGAGAGTGTTTCCAAAGTGCTTTGGCTGGATGAGATACAGCAAGCCGTCGATGAGGCCAACGTGGACGAGGACAGAGCAAAACAATGGGTTACTCTGGTGGTTGATGTTAATCAGTGTTTGGAAGGAAAAAAATCAAGTGATATTTTGTCTGTATTGAAGTCTTCCACTTCTAATGCAAATGACATAATCCCGGAGTGTGCTGACAAATACTATGATGCCCTTGTGAAGGCAAAAGAGCTCAAATCTGAAAGAGTGTCTAGTGACGGTTCATGGCTCAAACTCAACCTGCACAAAAAATATGACTACTATTACAACACTGATTCAAAAGAGAGTTCCTGGGTCACACCTGAATCATGCTTCTATAAAGAATCATGGCTCACAGGAAAAGAAATCGAGGACATTATTGAGGAAGTCACAGTAGGTTACATTCGTGAGAATATATGGTCTGCTTCAGAAGAGTTGCTTCTTCGCTTTCAAGCCACAAGCTCAGGACCCATCCTTAGGGAAGAGTTTGAAGCTAGAAAATCATTTTTGCATGAACAAGAAGAGAATGTGGTCAAAATACAGGCTTTTTGGAAAGGATATAAACAACGGAAGGAGTATATGCACAGGCGGCAAACGTTCATTGATAATACTGATTCTGTTGTGAAGATTCAGTCCTGGTTCCGAATGGCAACTGCAAGAAAGAGCTATCTTTCAAGACTACAGTATTTCAGAGATCATAATAATGAAATTGTGAAAATACAGTCACTGTTGAGAGCGAACAAAGCTAGAGATGACTACAAAACATTGGTTGGCTCTGAAAACCCACCATTAACAGTAATTCGCAAATTTGTATACCTGCTGGACCAAAGTGATTTGGATTTCCAGGAGGAACTAGAGGTTGCACGATTAAGGGAAGAAGTAGTGACCAAGATCAGGGCCAATCAACAGCTGGAAAAAGACCTGAACCTGATGGACATCAAGATTGGACTGCTGGTGAAGAACAGGATCACACTAGAGGATGTAATTTCACACAGTAAAAAGCTGAACAAGAAAAAAGGAGGAGAAATGGAAATACTGAATAACACCGACAACCAAGGAATAAAAAGTTTGAGTAAGGAGAGGAGAAAAACACTAGAAACATATCAGCAGCTGTTTTACCTTTTACAGACCAACCCTTTATACTTGGCTAAGCTGATTTTCCAGATGCCACAGAACAAGTCCACTAAATTTATGGATACTGTTATTTTCACACTATATAATTATGCCTCTAATCAGCGAGAAGAATATCTACTTCTCAAGCTTTTTAAAACTGCTCTGGAGGAAGAAATAAAATCAAAAGTGGACCAGGTACAGGACATAGTTACTGGTAACCCTACAGTCATCAAGATGGTCGTCAGCTTCAATAGAGGTGCCCGGGGACAGAACACCCTGCGCCAACTCCTGGCTCCAGTGGTAAAAGAGATCATCGACGACAAGTCGCTGATTATCAACACAAACCCTGTAGAGGTGTACAAGGCTTGGGTGAACCAACTAGAAACACAGACTGGAGAGGCCAGCAAGTTGCCTTATGATGTGACCACAGAACAAGCTCTAACATACCCAGAAGTGAAAAATAAACTGGAGGCTTCCATTGAGAACCTGAGAAGGGTCACCGACAAAGTCCTGAATTCTATCATTTCTTCCCTTGATCTACTGCCTTATGGATTGAGGTATATAGCCAAAGTACTGAAGAATTCGATCCATGAGAAATTCCCCGATGCAACAGAAGATGAGCTATTAAAGATTGTTGGAAACCTCCTGTACTATCGGTACATGAATCCAGCCATTGTAGCTCCAGATGGCTTTGATATCATCGACATGACAGCTGGAGGTCAGATAAATTCTGACCAAAGGAGAAACTTAGGATCAGTGGCCAAGGTTCTTCAGCACGCAGCCTCCAACAAGCTGTTTGAAGGAGAAAATGAGCATCTCTCATCTATGAACAATTATTTATCAGAGACGTATCAGGAATTCAGGAAATATTTCAAAGAAGCATGTAATGTCCCTGAGCCAGAAGAGAAGTTTAATATGGACAAATACACAGACCTGGTGACAGTCAGCAAACCAGTCATTTATATTTCAATTGAAGAAATCATCAGCACACACTCACTCCTGTTGGAACACCAGGATGCAATTGCCCCTGAGAAAAATGACTTACTGAGTGAATTGCTGGGGTCGCTGGGAGAGGTGCCAACCGTGGAATCTTTTCTTGGGGAAGGAGCAGTTGACCCCAATGACCCTAACAAGGCAAATACACTAAGTCAGCTTTCAAAGACCGAGATTTCTCTTGTCTTGACAAGCAAATATGACATAGAGGACGGTGAAGCTATAGATAGCCGAAGCCTCATGATAAAGACCAAGAAGCTGATAATTGATGTGATCCGGAACCAGCCAGGGAACACATTGACAGAAATCTTAGAGACACCAGCAACTGCGCAACAGGAGGTAGACCATGCCACGGACATGGTGAGCCGTGCAATGATAGATTCCAGGACTCCAGAAGAAATGAAGCATAGCCAATCTATGATTGAAGATGCACAGCTGCCTCTTGAGCAGAAGAAGAGGAAAATCCAGAGGAATCTTCGGACGTTGGAACAGACTGGACACGTGTCATCCGAAAATAAATACCAAGACATTCTCAATGAGATTGCCAAGGATATTCGAAATCAAAGAATCTATCGTAAGCTTCGAAAAGCTGAATTGGCAAAACTTCAGCAGACCCTGAATGCACTTAACAAGAAGGCAGCATTTTATGAAGAGCAAATCAATTATTATGACACCTACATAAAGACTTGTTTAGACAACTTAAAAAGAAAAAATACTCGGAGATCAATTAAACTAGATGGAAAAGGAGAACCCAAAGGGGCGAAGAGAGCGAAGCCAGTGAAGTACACTGCAGCAAAGCTGCATGAGAAAGGTGTCCTGCTAGATATAGATGATCTTCAAACAAACCAGTTTAAGAATGTTACATTTGATATCATAGCTACTGAAGATGTAGGCATTTTCGATGTAAGATCAAAATTCCTTGGTGTTGAGATGGAAAAGGTGCAACTCAATATTCAGGATTTACTTCAGATGCAATATGAAGGAGTAGCTGTAATGAAAATGTTTGATAAGGTTAAAGTGAATGTAAACCTTCTCATATACCTGCTGAACAAGAAGTTCTATGGAAAGTGAAGTGCCTACAGAAATTTCTTGGATTCTGTATCATCTGGATTAGGAAATGAATTTGTTTAATATTTTTGTTTTTAAACATGATTGAAATCACTGCTTATAAATGTGTGATTTTTTTTAAATGACCAAAACTGTTCTGAAGAATGTACCCAGGTGCCTTTTTGCTAATTTGATACTATAATAGAATGAGACATAAAATGAATTAATGGAAACATATCCACACTGTACTGTGATATAGGTACTCTGATTTAAAACTTTGGACATCCTGTGATCTGTTTTAAAGTTGGGGGGTGGGAAATTTAGCTGACTAGGGACAAACATGTAAACCTATTTTCCTATGAAAAAAGTTTTAAATGTCCCACTTGAATAACGTAATTCTTCATAGTTTTTTTAATCTATGGATAAATGGAAACCTAATTATTTGTAATGAATTATTTAGACAGTTCTAAGCCCTGTCTTCTGGGAGTTATCAATTTTAAAGAGAACTTTTGTGCAATTCAAATGAAGTTTTTATAAGTAATTGAAAATGACAACACAATAACACTTTCTGTATAAAAGTATATATTTTATGTGATTTATTCCTACTAAATGAAAGTGCACTACTGCCTCATGTAAAGACTCTTGCACGCAGAGCCTTTAAGTGACTAAGGAACAACATAGATAGTGAGCATAGTCCCCACCTCCACCCCTCACAATTTATTTGAATACTTCAATTGTGCCTCTCAATTTTTTGTAATGCTAAAAAATCAGTATCTAGATGGTTTTTAAATGTATTCTCTGGAAATTGTTTTATGTAAAATAAATGTTACTTAATTCCATT PRIMER_LEFT_EXPLAIN=considered 1, ok 1 PRIMER_RIGHT_EXPLAIN=considered 49099, GC content failed 2042, low tm 18100, high tm 10315, high end compl 16, long poly-x seq 226, high 3' stability 109, ok 18291 PRIMER_PAIR_EXPLAIN=considered 20, ok 20 PRIMER_STOP_CODON_POSITION=4947 PRIMER_PAIR_PENALTY=10.1097 PRIMER_LEFT_PENALTY=3.573461 PRIMER_RIGHT_PENALTY=6.536286 PRIMER_LEFT_SEQUENCE=ATGCCACACGAAGAGCTGC PRIMER_RIGHT_SEQUENCE=ACTTTCCATAGAACTTCTTGTTCAGC PRIMER_LEFT=222,19 PRIMER_RIGHT=4947,26 PRIMER_LEFT_TM=62.573 PRIMER_RIGHT_TM=60.536 PRIMER_LEFT_GC_PERCENT=57.895 PRIMER_RIGHT_GC_PERCENT=38.462 PRIMER_LEFT_SELF_ANY=4.00 PRIMER_RIGHT_SELF_ANY=7.00 PRIMER_LEFT_SELF_END=3.00 PRIMER_RIGHT_SELF_END=3.00 PRIMER_LEFT_POSITION_PENALTY=0.000000 PRIMER_RIGHT_POSITION_PENALTY=0.000000 PRIMER_LEFT_END_STABILITY=9.7000 PRIMER_RIGHT_END_STABILITY=8.2000 PRIMER_PAIR_COMPL_ANY=6.00 PRIMER_PAIR_COMPL_END=3.00 PRIMER_PRODUCT_SIZE=4726 PRIMER_PAIR_PENALTY_1=10.1546 PRIMER_LEFT_1_PENALTY=3.573461 PRIMER_RIGHT_1_PENALTY=6.581149 PRIMER_LEFT_1_SEQUENCE=ATGCCACACGAAGAGCTGC PRIMER_RIGHT_1_SEQUENCE=CACTTTCCATAGAACTTCTTGTTCA PRIMER_LEFT_1=222,19 PRIMER_RIGHT_1=4948,25 PRIMER_LEFT_1_TM=62.573 PRIMER_RIGHT_1_TM=58.919 PRIMER_LEFT_1_GC_PERCENT=57.895 PRIMER_RIGHT_1_GC_PERCENT=36.000 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=7.00 PRIMER_LEFT_1_SELF_END=3.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_LEFT_1_POSITION_PENALTY=0.000000 PRIMER_RIGHT_1_POSITION_PENALTY=0.500000 PRIMER_LEFT_1_END_STABILITY=9.7000 PRIMER_RIGHT_1_END_STABILITY=6.7000 PRIMER_PAIR_1_COMPL_ANY=6.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PRODUCT_SIZE_1=4727 PRIMER_PAIR_PENALTY_2=11.1029 PRIMER_LEFT_2_PENALTY=3.573461 PRIMER_RIGHT_2_PENALTY=7.529417 PRIMER_LEFT_2_SEQUENCE=ATGCCACACGAAGAGCTGC PRIMER_RIGHT_2_SEQUENCE=TCACTTTCCATAGAACTTCTTGTTCA PRIMER_LEFT_2=222,19 PRIMER_RIGHT_2=4949,26 PRIMER_LEFT_2_TM=62.573 PRIMER_RIGHT_2_TM=60.529 PRIMER_LEFT_2_GC_PERCENT=57.895 PRIMER_RIGHT_2_GC_PERCENT=34.615 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=7.00 PRIMER_LEFT_2_SELF_END=3.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_LEFT_2_POSITION_PENALTY=0.000000 PRIMER_RIGHT_2_POSITION_PENALTY=1.000000 PRIMER_LEFT_2_END_STABILITY=9.7000 PRIMER_RIGHT_2_END_STABILITY=6.7000 PRIMER_PAIR_2_COMPL_ANY=6.00 PRIMER_PAIR_2_COMPL_END=3.00 PRIMER_PRODUCT_SIZE_2=4728 PRIMER_PAIR_PENALTY_3=11.4796 PRIMER_LEFT_3_PENALTY=3.573461 PRIMER_RIGHT_3_PENALTY=7.906112 PRIMER_LEFT_3_SEQUENCE=ATGCCACACGAAGAGCTGC PRIMER_RIGHT_3_SEQUENCE=TCACTTTCCATAGAACTTCTTGTTC PRIMER_LEFT_3=222,19 PRIMER_RIGHT_3=4949,25 PRIMER_LEFT_3_TM=62.573 PRIMER_RIGHT_3_TM=58.094 PRIMER_LEFT_3_GC_PERCENT=57.895 PRIMER_RIGHT_3_GC_PERCENT=36.000 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=7.00 PRIMER_LEFT_3_SELF_END=3.00 PRIMER_RIGHT_3_SELF_END=3.00 PRIMER_LEFT_3_POSITION_PENALTY=0.000000 PRIMER_RIGHT_3_POSITION_PENALTY=1.000000 PRIMER_LEFT_3_END_STABILITY=9.7000 PRIMER_RIGHT_3_END_STABILITY=6.7000 PRIMER_PAIR_3_COMPL_ANY=6.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_3=4728 PRIMER_PAIR_PENALTY_4=11.7334 PRIMER_LEFT_4_PENALTY=3.573461 PRIMER_RIGHT_4_PENALTY=8.159919 PRIMER_LEFT_4_SEQUENCE=ATGCCACACGAAGAGCTGC PRIMER_RIGHT_4_SEQUENCE=GGCACTTCACTTTCCATAGAACT PRIMER_LEFT_4=222,19 PRIMER_RIGHT_4=4955,23 PRIMER_LEFT_4_TM=62.573 PRIMER_RIGHT_4_TM=58.840 PRIMER_LEFT_4_GC_PERCENT=57.895 PRIMER_RIGHT_4_GC_PERCENT=43.478 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=3.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_LEFT_4_POSITION_PENALTY=0.000000 PRIMER_RIGHT_4_POSITION_PENALTY=4.000000 PRIMER_LEFT_4_END_STABILITY=9.7000 PRIMER_RIGHT_4_END_STABILITY=6.4000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_4=4734 PRIMER_PAIR_PENALTY_5=11.7730 PRIMER_LEFT_5_PENALTY=3.573461 PRIMER_RIGHT_5_PENALTY=8.199569 PRIMER_LEFT_5_SEQUENCE=ATGCCACACGAAGAGCTGC PRIMER_RIGHT_5_SEQUENCE=TTCACTTTCCATAGAACTTCTTGTTC PRIMER_LEFT_5=222,19 PRIMER_RIGHT_5=4950,26 PRIMER_LEFT_5_TM=62.573 PRIMER_RIGHT_5_TM=59.300 PRIMER_LEFT_5_GC_PERCENT=57.895 PRIMER_RIGHT_5_GC_PERCENT=34.615 PRIMER_LEFT_5_SELF_ANY=4.00 PRIMER_RIGHT_5_SELF_ANY=7.00 PRIMER_LEFT_5_SELF_END=3.00 PRIMER_RIGHT_5_SELF_END=3.00 PRIMER_LEFT_5_POSITION_PENALTY=0.000000 PRIMER_RIGHT_5_POSITION_PENALTY=1.500000 PRIMER_LEFT_5_END_STABILITY=9.7000 PRIMER_RIGHT_5_END_STABILITY=6.7000 PRIMER_PAIR_5_COMPL_ANY=6.00 PRIMER_PAIR_5_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_5=4729 PRIMER_PAIR_PENALTY_6=12.3404 PRIMER_LEFT_6_PENALTY=3.573461 PRIMER_RIGHT_6_PENALTY=8.766928 PRIMER_LEFT_6_SEQUENCE=ATGCCACACGAAGAGCTGC PRIMER_RIGHT_6_SEQUENCE=AGGCACTTCACTTTCCATAGAACT PRIMER_LEFT_6=222,19 PRIMER_RIGHT_6=4956,24 PRIMER_LEFT_6_TM=62.573 PRIMER_RIGHT_6_TM=59.733 PRIMER_LEFT_6_GC_PERCENT=57.895 PRIMER_RIGHT_6_GC_PERCENT=41.667 PRIMER_LEFT_6_SELF_ANY=4.00 PRIMER_RIGHT_6_SELF_ANY=6.00 PRIMER_LEFT_6_SELF_END=3.00 PRIMER_RIGHT_6_SELF_END=2.00 PRIMER_LEFT_6_POSITION_PENALTY=0.000000 PRIMER_RIGHT_6_POSITION_PENALTY=4.500000 PRIMER_LEFT_6_END_STABILITY=9.7000 PRIMER_RIGHT_6_END_STABILITY=6.4000 PRIMER_PAIR_6_COMPL_ANY=5.00 PRIMER_PAIR_6_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_6=4735 PRIMER_PAIR_PENALTY_7=12.3811 PRIMER_LEFT_7_PENALTY=3.573461 PRIMER_RIGHT_7_PENALTY=8.807615 PRIMER_LEFT_7_SEQUENCE=ATGCCACACGAAGAGCTGC PRIMER_RIGHT_7_SEQUENCE=TTCACTTTCCATAGAACTTCTTGTT PRIMER_LEFT_7=222,19 PRIMER_RIGHT_7=4950,25 PRIMER_LEFT_7_TM=62.573 PRIMER_RIGHT_7_TM=57.692 PRIMER_LEFT_7_GC_PERCENT=57.895 PRIMER_RIGHT_7_GC_PERCENT=32.000 PRIMER_LEFT_7_SELF_ANY=4.00 PRIMER_RIGHT_7_SELF_ANY=7.00 PRIMER_LEFT_7_SELF_END=3.00 PRIMER_RIGHT_7_SELF_END=3.00 PRIMER_LEFT_7_POSITION_PENALTY=0.000000 PRIMER_RIGHT_7_POSITION_PENALTY=1.500000 PRIMER_LEFT_7_END_STABILITY=9.7000 PRIMER_RIGHT_7_END_STABILITY=7.0000 PRIMER_PAIR_7_COMPL_ANY=6.00 PRIMER_PAIR_7_COMPL_END=3.00 PRIMER_PRODUCT_SIZE_7=4729 PRIMER_PAIR_PENALTY_8=12.6598 PRIMER_LEFT_8_PENALTY=3.573461 PRIMER_RIGHT_8_PENALTY=9.086339 PRIMER_LEFT_8_SEQUENCE=ATGCCACACGAAGAGCTGC PRIMER_RIGHT_8_SEQUENCE=TCTGTAGGCACTTCACTTTCCA PRIMER_LEFT_8=222,19 PRIMER_RIGHT_8=4961,22 PRIMER_LEFT_8_TM=62.573 PRIMER_RIGHT_8_TM=59.914 PRIMER_LEFT_8_GC_PERCENT=57.895 PRIMER_RIGHT_8_GC_PERCENT=45.455 PRIMER_LEFT_8_SELF_ANY=4.00 PRIMER_RIGHT_8_SELF_ANY=4.00 PRIMER_LEFT_8_SELF_END=3.00 PRIMER_RIGHT_8_SELF_END=0.00 PRIMER_LEFT_8_POSITION_PENALTY=0.000000 PRIMER_RIGHT_8_POSITION_PENALTY=7.000000 PRIMER_LEFT_8_END_STABILITY=9.7000 PRIMER_RIGHT_8_END_STABILITY=8.5000 PRIMER_PAIR_8_COMPL_ANY=5.00 PRIMER_PAIR_8_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_8=4740 PRIMER_PAIR_PENALTY_9=12.6743 PRIMER_LEFT_9_PENALTY=3.573461 PRIMER_RIGHT_9_PENALTY=9.100880 PRIMER_LEFT_9_SEQUENCE=ATGCCACACGAAGAGCTGC PRIMER_RIGHT_9_SEQUENCE=CTTCACTTTCCATAGAACTTCTTGTTC PRIMER_LEFT_9=222,19 PRIMER_RIGHT_9=4951,27 PRIMER_LEFT_9_TM=62.573 PRIMER_RIGHT_9_TM=60.101 PRIMER_LEFT_9_GC_PERCENT=57.895 PRIMER_RIGHT_9_GC_PERCENT=37.037 PRIMER_LEFT_9_SELF_ANY=4.00 PRIMER_RIGHT_9_SELF_ANY=7.00 PRIMER_LEFT_9_SELF_END=3.00 PRIMER_RIGHT_9_SELF_END=3.00 PRIMER_LEFT_9_POSITION_PENALTY=0.000000 PRIMER_RIGHT_9_POSITION_PENALTY=2.000000 PRIMER_LEFT_9_END_STABILITY=9.7000 PRIMER_RIGHT_9_END_STABILITY=6.7000 PRIMER_PAIR_9_COMPL_ANY=6.00 PRIMER_PAIR_9_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_9=4730 PRIMER_PAIR_PENALTY_10=12.9973 PRIMER_LEFT_10_PENALTY=3.573461 PRIMER_RIGHT_10_PENALTY=9.423820 PRIMER_LEFT_10_SEQUENCE=ATGCCACACGAAGAGCTGC PRIMER_RIGHT_10_SEQUENCE=CTTCACTTTCCATAGAACTTCTTGTT PRIMER_LEFT_10=222,19 PRIMER_RIGHT_10=4951,26 PRIMER_LEFT_10_TM=62.573 PRIMER_RIGHT_10_TM=58.576 PRIMER_LEFT_10_GC_PERCENT=57.895 PRIMER_RIGHT_10_GC_PERCENT=34.615 PRIMER_LEFT_10_SELF_ANY=4.00 PRIMER_RIGHT_10_SELF_ANY=7.00 PRIMER_LEFT_10_SELF_END=3.00 PRIMER_RIGHT_10_SELF_END=3.00 PRIMER_LEFT_10_POSITION_PENALTY=0.000000 PRIMER_RIGHT_10_POSITION_PENALTY=2.000000 PRIMER_LEFT_10_END_STABILITY=9.7000 PRIMER_RIGHT_10_END_STABILITY=7.0000 PRIMER_PAIR_10_COMPL_ANY=6.00 PRIMER_PAIR_10_COMPL_END=3.00 PRIMER_PRODUCT_SIZE_10=4730 PRIMER_PAIR_PENALTY_11=13.0507 PRIMER_LEFT_11_PENALTY=3.573461 PRIMER_RIGHT_11_PENALTY=9.477278 PRIMER_LEFT_11_SEQUENCE=ATGCCACACGAAGAGCTGC PRIMER_RIGHT_11_SEQUENCE=CTGTAGGCACTTCACTTTCCA PRIMER_LEFT_11=222,19 PRIMER_RIGHT_11=4960,21 PRIMER_LEFT_11_TM=62.573 PRIMER_RIGHT_11_TM=58.023 PRIMER_LEFT_11_GC_PERCENT=57.895 PRIMER_RIGHT_11_GC_PERCENT=47.619 PRIMER_LEFT_11_SELF_ANY=4.00 PRIMER_RIGHT_11_SELF_ANY=4.00 PRIMER_LEFT_11_SELF_END=3.00 PRIMER_RIGHT_11_SELF_END=0.00 PRIMER_LEFT_11_POSITION_PENALTY=0.000000 PRIMER_RIGHT_11_POSITION_PENALTY=6.500000 PRIMER_LEFT_11_END_STABILITY=9.7000 PRIMER_RIGHT_11_END_STABILITY=8.5000 PRIMER_PAIR_11_COMPL_ANY=5.00 PRIMER_PAIR_11_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_11=4739 PRIMER_PAIR_PENALTY_12=13.2295 PRIMER_LEFT_12_PENALTY=3.573461 PRIMER_RIGHT_12_PENALTY=9.655999 PRIMER_LEFT_12_SEQUENCE=ATGCCACACGAAGAGCTGC PRIMER_RIGHT_12_SEQUENCE=TGTAGGCACTTCACTTTCCAT PRIMER_LEFT_12=222,19 PRIMER_RIGHT_12=4959,21 PRIMER_LEFT_12_TM=62.573 PRIMER_RIGHT_12_TM=57.344 PRIMER_LEFT_12_GC_PERCENT=57.895 PRIMER_RIGHT_12_GC_PERCENT=42.857 PRIMER_LEFT_12_SELF_ANY=4.00 PRIMER_RIGHT_12_SELF_ANY=4.00 PRIMER_LEFT_12_SELF_END=3.00 PRIMER_RIGHT_12_SELF_END=2.00 PRIMER_LEFT_12_POSITION_PENALTY=0.000000 PRIMER_RIGHT_12_POSITION_PENALTY=6.000000 PRIMER_LEFT_12_END_STABILITY=9.7000 PRIMER_RIGHT_12_END_STABILITY=8.1000 PRIMER_PAIR_12_COMPL_ANY=5.00 PRIMER_PAIR_12_COMPL_END=3.00 PRIMER_PRODUCT_SIZE_12=4738 PRIMER_PAIR_PENALTY_13=13.3361 PRIMER_LEFT_13_PENALTY=3.573461 PRIMER_RIGHT_13_PENALTY=9.762661 PRIMER_LEFT_13_SEQUENCE=ATGCCACACGAAGAGCTGC PRIMER_RIGHT_13_SEQUENCE=ACTTTCCATAGAACTTCTTGTTCAGCA PRIMER_LEFT_13=222,19 PRIMER_RIGHT_13=4947,27 PRIMER_LEFT_13_TM=62.573 PRIMER_RIGHT_13_TM=62.763 PRIMER_LEFT_13_GC_PERCENT=57.895 PRIMER_RIGHT_13_GC_PERCENT=37.037 PRIMER_LEFT_13_SELF_ANY=4.00 PRIMER_RIGHT_13_SELF_ANY=7.00 PRIMER_LEFT_13_SELF_END=3.00 PRIMER_RIGHT_13_SELF_END=3.00 PRIMER_LEFT_13_POSITION_PENALTY=0.000000 PRIMER_RIGHT_13_POSITION_PENALTY=0.000000 PRIMER_LEFT_13_END_STABILITY=9.7000 PRIMER_RIGHT_13_END_STABILITY=8.5000 PRIMER_PAIR_13_COMPL_ANY=6.00 PRIMER_PAIR_13_COMPL_END=3.00 PRIMER_PRODUCT_SIZE_13=4726 PRIMER_PAIR_PENALTY_14=13.6715 PRIMER_LEFT_14_PENALTY=3.573461 PRIMER_RIGHT_14_PENALTY=10.098012 PRIMER_LEFT_14_SEQUENCE=ATGCCACACGAAGAGCTGC PRIMER_RIGHT_14_SEQUENCE=TTCACTTTCCATAGAACTTCTTGTTCA PRIMER_LEFT_14=222,19 PRIMER_RIGHT_14=4950,27 PRIMER_LEFT_14_TM=62.573 PRIMER_RIGHT_14_TM=61.598 PRIMER_LEFT_14_GC_PERCENT=57.895 PRIMER_RIGHT_14_GC_PERCENT=33.333 PRIMER_LEFT_14_SELF_ANY=4.00 PRIMER_RIGHT_14_SELF_ANY=7.00 PRIMER_LEFT_14_SELF_END=3.00 PRIMER_RIGHT_14_SELF_END=3.00 PRIMER_LEFT_14_POSITION_PENALTY=0.000000 PRIMER_RIGHT_14_POSITION_PENALTY=1.500000 PRIMER_LEFT_14_END_STABILITY=9.7000 PRIMER_RIGHT_14_END_STABILITY=6.7000 PRIMER_PAIR_14_COMPL_ANY=6.00 PRIMER_PAIR_14_COMPL_END=3.00 PRIMER_PRODUCT_SIZE_14=4729 PRIMER_PAIR_PENALTY_15=13.6784 PRIMER_LEFT_15_PENALTY=3.573461 PRIMER_RIGHT_15_PENALTY=10.104943 PRIMER_LEFT_15_SEQUENCE=ATGCCACACGAAGAGCTGC PRIMER_RIGHT_15_SEQUENCE=CTGTAGGCACTTCACTTTCCAT PRIMER_LEFT_15=222,19 PRIMER_RIGHT_15=4960,22 PRIMER_LEFT_15_TM=62.573 PRIMER_RIGHT_15_TM=58.395 PRIMER_LEFT_15_GC_PERCENT=57.895 PRIMER_RIGHT_15_GC_PERCENT=45.455 PRIMER_LEFT_15_SELF_ANY=4.00 PRIMER_RIGHT_15_SELF_ANY=4.00 PRIMER_LEFT_15_SELF_END=3.00 PRIMER_RIGHT_15_SELF_END=2.00 PRIMER_LEFT_15_POSITION_PENALTY=0.000000 PRIMER_RIGHT_15_POSITION_PENALTY=6.500000 PRIMER_LEFT_15_END_STABILITY=9.7000 PRIMER_RIGHT_15_END_STABILITY=8.1000 PRIMER_PAIR_15_COMPL_ANY=5.00 PRIMER_PAIR_15_COMPL_END=3.00 PRIMER_PRODUCT_SIZE_15=4739 PRIMER_PAIR_PENALTY_16=13.7519 PRIMER_LEFT_16_PENALTY=3.573461 PRIMER_RIGHT_16_PENALTY=10.178426 PRIMER_LEFT_16_SEQUENCE=ATGCCACACGAAGAGCTGC PRIMER_RIGHT_16_SEQUENCE=TCTGTAGGCACTTCACTTTCCAT PRIMER_LEFT_16=222,19 PRIMER_RIGHT_16=4961,23 PRIMER_LEFT_16_TM=62.573 PRIMER_RIGHT_16_TM=60.178 PRIMER_LEFT_16_GC_PERCENT=57.895 PRIMER_RIGHT_16_GC_PERCENT=43.478 PRIMER_LEFT_16_SELF_ANY=4.00 PRIMER_RIGHT_16_SELF_ANY=4.00 PRIMER_LEFT_16_SELF_END=3.00 PRIMER_RIGHT_16_SELF_END=2.00 PRIMER_LEFT_16_POSITION_PENALTY=0.000000 PRIMER_RIGHT_16_POSITION_PENALTY=7.000000 PRIMER_LEFT_16_END_STABILITY=9.7000 PRIMER_RIGHT_16_END_STABILITY=8.1000 PRIMER_PAIR_16_COMPL_ANY=5.00 PRIMER_PAIR_16_COMPL_END=3.00 PRIMER_PRODUCT_SIZE_16=4740 PRIMER_PAIR_PENALTY_17=13.7905 PRIMER_LEFT_17_PENALTY=3.573461 PRIMER_RIGHT_17_PENALTY=10.217023 PRIMER_LEFT_17_SEQUENCE=ATGCCACACGAAGAGCTGC PRIMER_RIGHT_17_SEQUENCE=ACTTCACTTTCCATAGAACTTCTTGTT PRIMER_LEFT_17=222,19 PRIMER_RIGHT_17=4952,27 PRIMER_LEFT_17_TM=62.573 PRIMER_RIGHT_17_TM=59.283 PRIMER_LEFT_17_GC_PERCENT=57.895 PRIMER_RIGHT_17_GC_PERCENT=33.333 PRIMER_LEFT_17_SELF_ANY=4.00 PRIMER_RIGHT_17_SELF_ANY=7.00 PRIMER_LEFT_17_SELF_END=3.00 PRIMER_RIGHT_17_SELF_END=3.00 PRIMER_LEFT_17_POSITION_PENALTY=0.000000 PRIMER_RIGHT_17_POSITION_PENALTY=2.500000 PRIMER_LEFT_17_END_STABILITY=9.7000 PRIMER_RIGHT_17_END_STABILITY=7.0000 PRIMER_PAIR_17_COMPL_ANY=6.00 PRIMER_PAIR_17_COMPL_END=3.00 PRIMER_PRODUCT_SIZE_17=4731 PRIMER_PAIR_PENALTY_18=13.8224 PRIMER_LEFT_18_PENALTY=3.573461 PRIMER_RIGHT_18_PENALTY=10.248922 PRIMER_LEFT_18_SEQUENCE=ATGCCACACGAAGAGCTGC PRIMER_RIGHT_18_SEQUENCE=TAGGCACTTCACTTTCCATAGAACT PRIMER_LEFT_18=222,19 PRIMER_RIGHT_18=4957,25 PRIMER_LEFT_18_TM=62.573 PRIMER_RIGHT_18_TM=59.751 PRIMER_LEFT_18_GC_PERCENT=57.895 PRIMER_RIGHT_18_GC_PERCENT=40.000 PRIMER_LEFT_18_SELF_ANY=4.00 PRIMER_RIGHT_18_SELF_ANY=6.00 PRIMER_LEFT_18_SELF_END=3.00 PRIMER_RIGHT_18_SELF_END=2.00 PRIMER_LEFT_18_POSITION_PENALTY=0.000000 PRIMER_RIGHT_18_POSITION_PENALTY=5.000000 PRIMER_LEFT_18_END_STABILITY=9.7000 PRIMER_RIGHT_18_END_STABILITY=6.4000 PRIMER_PAIR_18_COMPL_ANY=5.00 PRIMER_PAIR_18_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_18=4736 PRIMER_PAIR_PENALTY_19=13.8361 PRIMER_LEFT_19_PENALTY=3.573461 PRIMER_RIGHT_19_PENALTY=10.262661 PRIMER_LEFT_19_SEQUENCE=ATGCCACACGAAGAGCTGC PRIMER_RIGHT_19_SEQUENCE=CACTTTCCATAGAACTTCTTGTTCAGC PRIMER_LEFT_19=222,19 PRIMER_RIGHT_19=4948,27 PRIMER_LEFT_19_TM=62.573 PRIMER_RIGHT_19_TM=62.763 PRIMER_LEFT_19_GC_PERCENT=57.895 PRIMER_RIGHT_19_GC_PERCENT=40.741 PRIMER_LEFT_19_SELF_ANY=4.00 PRIMER_RIGHT_19_SELF_ANY=7.00 PRIMER_LEFT_19_SELF_END=3.00 PRIMER_RIGHT_19_SELF_END=3.00 PRIMER_LEFT_19_POSITION_PENALTY=0.000000 PRIMER_RIGHT_19_POSITION_PENALTY=0.500000 PRIMER_LEFT_19_END_STABILITY=9.7000 PRIMER_RIGHT_19_END_STABILITY=8.2000 PRIMER_PAIR_19_COMPL_ANY=6.00 PRIMER_PAIR_19_COMPL_END=3.00 PRIMER_PRODUCT_SIZE_19=4727 = PRIMER_SEQUENCE_ID=M59040/CD44 PRIMER_COMMENT=Human cell adhesion molecule (CD44) mRNA, complete cds. CDS=29..1114 PRIMER_START_CODON_POSITION=28 SEQUENCE=CCCGCGCCCTCCGTTCGCTCCGGACACCATGGACAAGTTTTGGTGGCACGCAGCCTGGGGACTCTGCCTCGTGCCGCTGAGCCTGGCGCAGATCGATTTGAATATAACCTGCCGCTTTGCAGGTGTATTCCACGTGGAGAAAAATGGTCGCTACAGCATCTCTCGGACGGAGGCCGCTGACCTCTGCAAGGCTTTCAATAGCACCTTGCCCACAATGGCCCAGATGGAGAAAGCTCTGAGCATCGGATTTGAGACCTGCAGGTATGGGTTCATAGAAGGGCATGTGGTGATTCCCCGGATCCACCCCAACTCCATCTGTGCAGCAAACAACACAGGGGTGTACATCCTCACATACAACACCTCCCAGTATGACACATATTGCTTCAATGCTTCAGCTCCACCTGAAGAAGATTGTACATCAGTCACAGACCTGCCCAATGCCTTTGATGGACCAATTACCATAACTATTGTTAACCGTGATGGCACCCGCTATGTCCAGAAAGGAGAATACAGAACGAATCCTGAAGACATCTACCCCAGCAACCCTACTGATGATGACGTGAGCAGCGGCTCCTCCAGTGAAAGGAGCAGCACTTCAGGAGGTTACATCTTTTACACCTTTTCTACTGTACACCCCATCCCAGACGAAGACAGTCCCTGGATCACCGACAGCACAGACAGAATCCCTGCTACCAGAGACCAAGACACATTCCACCCCAGTGGGGGGTCCCATACCACTCATGGATCTGAATCAGATGGACACTCACATGGGAGTCAAGAAGGTGGAGCAAACACAACCTCTGGTCCTATAAGGACACCCCAAATTCCAGAATGGCTGATCATCTTGGCATCCCTCTTGGCCTTGGCTTTGATTCTTGCAGTTTGCATTGCAGTCAACAGTCGAAGAAGGTGTGGGCAGAAGAAAAAGCTAGTGATCAACAGTGGCAATGGAGCTGTGGAGGACAGAAAGCCAAGTGGACTCAACGGAGAGGCCAGCAAGTCTCAGGAAATGGTGCATTTGGTGAACAAGGAGTCGTCAGAAACTCCAGACCAGTTTATGACAGCTGATGAGACAAGGAACCTGCAGAATGTGGACATGAAGATTGGGGTGTAACACCTACACCATTATCTTGGAAAGAAACAACCGTTGGAAACATAACCATTACAGGGAGCTGGGACACTTAACAGATGCAATGTGCTACTGATTGTTTCATTGCGAATCTTTTTTAGCATAAAATTTTCTACTCTTTTTGTTTTTTGTGTTTTGTTCTTTAAAGTCAGGTCCAATTTGTAAAAACAGCATTGCTTTGTAAATTAGGGCCCAATTAATAATCAGCAAGAATTTGATCGTTCAGTTCCACTTGGAGGCCTTCATCCTCGGGTGTGCTATGGATGGCTTCTAACAAAAACTACACATATGTATTCCTGATCGCCAACCTTTCCCCCACCAGCTAAGGACATTTCCCAGGGTTAATAGGGCCTGGTCCCTGGGAGGAAATTTGAATGGGTCCATTTTGCCCTTCCATAGCCTAATCCCTGGGCATTGCTTTCCACTGAGGTTGGGGTGTACTAGTTACACATCTTCAACAGACCCCCTCTAGAAATTTTTCAGATGCTTCTGGGAGACACCAAAGGGTGAAGCTATTTATCTGTAGTAAACTATTTATCTGTGTTTTTGAAATATTAAACCCTGGATCAGTCCTTTGATCAGTATAATTTTTTAAAGTTACTTTGTCAGAGGCACAAAAGGGTTTAAACTGATTCATAATAAATATCTGTACTTCTTCGATCTTC PRIMER_LEFT_EXPLAIN=considered 15178, would not amplify any of the ORF 11930, GC content failed 2, low tm 495, high tm 1516, high end compl 1, long poly-x seq 22, high 3' stability 27, ok 1185 PRIMER_RIGHT_EXPLAIN=considered 14630, GC content failed 362, low tm 3262, high tm 5355, high any compl 3, high end compl 2, long poly-x seq 73, high 3' stability 60, ok 5513 PRIMER_PAIR_EXPLAIN=considered 22, high end compl 1, ok 21 PRIMER_STOP_CODON_POSITION=1111 PRIMER_PAIR_PENALTY=1.6996 PRIMER_LEFT_PENALTY=1.335843 PRIMER_RIGHT_PENALTY=0.363753 PRIMER_LEFT_SEQUENCE=ATGGACAAGTTTTGGTGGCA PRIMER_RIGHT_SEQUENCE=ACACCCCAATCTTCATGTCC PRIMER_LEFT=28,20 PRIMER_RIGHT=1111,20 PRIMER_LEFT_TM=61.336 PRIMER_RIGHT_TM=59.636 PRIMER_LEFT_GC_PERCENT=45.000 PRIMER_RIGHT_GC_PERCENT=50.000 PRIMER_LEFT_SELF_ANY=3.00 PRIMER_RIGHT_SELF_ANY=4.00 PRIMER_LEFT_SELF_END=3.00 PRIMER_RIGHT_SELF_END=0.00 PRIMER_LEFT_POSITION_PENALTY=0.000000 PRIMER_RIGHT_POSITION_PENALTY=0.000000 PRIMER_LEFT_END_STABILITY=10.0000 PRIMER_RIGHT_END_STABILITY=7.9000 PRIMER_PAIR_COMPL_ANY=5.00 PRIMER_PAIR_COMPL_END=2.00 PRIMER_PRODUCT_SIZE=1084 PRIMER_PAIR_PENALTY_1=2.9624 PRIMER_LEFT_1_PENALTY=2.598622 PRIMER_RIGHT_1_PENALTY=0.363753 PRIMER_LEFT_1_SEQUENCE=ATGGACAAGTTTTGGTGGC PRIMER_RIGHT_1_SEQUENCE=ACACCCCAATCTTCATGTCC PRIMER_LEFT_1=28,19 PRIMER_RIGHT_1=1111,20 PRIMER_LEFT_1_TM=58.401 PRIMER_RIGHT_1_TM=59.636 PRIMER_LEFT_1_GC_PERCENT=47.368 PRIMER_RIGHT_1_GC_PERCENT=50.000 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_LEFT_1_POSITION_PENALTY=0.000000 PRIMER_RIGHT_1_POSITION_PENALTY=0.000000 PRIMER_LEFT_1_END_STABILITY=9.4000 PRIMER_RIGHT_1_END_STABILITY=7.9000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_1=1084 PRIMER_PAIR_PENALTY_2=3.1742 PRIMER_LEFT_2_PENALTY=1.335843 PRIMER_RIGHT_2_PENALTY=1.838405 PRIMER_LEFT_2_SEQUENCE=ATGGACAAGTTTTGGTGGCA PRIMER_RIGHT_2_SEQUENCE=TACACCCCAATCTTCATGTCC PRIMER_LEFT_2=28,20 PRIMER_RIGHT_2=1112,21 PRIMER_LEFT_2_TM=61.336 PRIMER_RIGHT_2_TM=59.662 PRIMER_LEFT_2_GC_PERCENT=45.000 PRIMER_RIGHT_2_GC_PERCENT=47.619 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=3.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_LEFT_2_POSITION_PENALTY=0.000000 PRIMER_RIGHT_2_POSITION_PENALTY=0.500000 PRIMER_LEFT_2_END_STABILITY=10.0000 PRIMER_RIGHT_2_END_STABILITY=7.9000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_2=1085 PRIMER_PAIR_PENALTY_3=4.4370 PRIMER_LEFT_3_PENALTY=2.598622 PRIMER_RIGHT_3_PENALTY=1.838405 PRIMER_LEFT_3_SEQUENCE=ATGGACAAGTTTTGGTGGC PRIMER_RIGHT_3_SEQUENCE=TACACCCCAATCTTCATGTCC PRIMER_LEFT_3=28,19 PRIMER_RIGHT_3=1112,21 PRIMER_LEFT_3_TM=58.401 PRIMER_RIGHT_3_TM=59.662 PRIMER_LEFT_3_GC_PERCENT=47.368 PRIMER_RIGHT_3_GC_PERCENT=47.619 PRIMER_LEFT_3_SELF_ANY=3.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_LEFT_3_POSITION_PENALTY=0.000000 PRIMER_RIGHT_3_POSITION_PENALTY=0.500000 PRIMER_LEFT_3_END_STABILITY=9.4000 PRIMER_RIGHT_3_END_STABILITY=7.9000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_3=1085 PRIMER_PAIR_PENALTY_4=4.8500 PRIMER_LEFT_4_PENALTY=1.335843 PRIMER_RIGHT_4_PENALTY=3.514144 PRIMER_LEFT_4_SEQUENCE=ATGGACAAGTTTTGGTGGCA PRIMER_RIGHT_4_SEQUENCE=ACACCCCAATCTTCATGTCCA PRIMER_LEFT_4=28,20 PRIMER_RIGHT_4=1111,21 PRIMER_LEFT_4_TM=61.336 PRIMER_RIGHT_4_TM=62.514 PRIMER_LEFT_4_GC_PERCENT=45.000 PRIMER_RIGHT_4_GC_PERCENT=47.619 PRIMER_LEFT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=3.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_LEFT_4_POSITION_PENALTY=0.000000 PRIMER_RIGHT_4_POSITION_PENALTY=0.000000 PRIMER_LEFT_4_END_STABILITY=10.0000 PRIMER_RIGHT_4_END_STABILITY=7.9000 PRIMER_PAIR_4_COMPL_ANY=6.00 PRIMER_PAIR_4_COMPL_END=3.00 PRIMER_PRODUCT_SIZE_4=1084 PRIMER_PAIR_PENALTY_5=5.3038 PRIMER_LEFT_5_PENALTY=1.335843 PRIMER_RIGHT_5_PENALTY=3.967916 PRIMER_LEFT_5_SEQUENCE=ATGGACAAGTTTTGGTGGCA PRIMER_RIGHT_5_SEQUENCE=TTACACCCCAATCTTCATGTCC PRIMER_LEFT_5=28,20 PRIMER_RIGHT_5=1113,22 PRIMER_LEFT_5_TM=61.336 PRIMER_RIGHT_5_TM=60.968 PRIMER_LEFT_5_GC_PERCENT=45.000 PRIMER_RIGHT_5_GC_PERCENT=45.455 PRIMER_LEFT_5_SELF_ANY=3.00 PRIMER_RIGHT_5_SELF_ANY=4.00 PRIMER_LEFT_5_SELF_END=3.00 PRIMER_RIGHT_5_SELF_END=0.00 PRIMER_LEFT_5_POSITION_PENALTY=0.000000 PRIMER_RIGHT_5_POSITION_PENALTY=1.000000 PRIMER_LEFT_5_END_STABILITY=10.0000 PRIMER_RIGHT_5_END_STABILITY=7.9000 PRIMER_PAIR_5_COMPL_ANY=5.00 PRIMER_PAIR_5_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_5=1086 PRIMER_PAIR_PENALTY_6=5.9592 PRIMER_LEFT_6_PENALTY=1.335843 PRIMER_RIGHT_6_PENALTY=4.623327 PRIMER_LEFT_6_SEQUENCE=ATGGACAAGTTTTGGTGGCA PRIMER_RIGHT_6_SEQUENCE=TTACACCCCAATCTTCATGTC PRIMER_LEFT_6=28,20 PRIMER_RIGHT_6=1113,21 PRIMER_LEFT_6_TM=61.336 PRIMER_RIGHT_6_TM=57.377 PRIMER_LEFT_6_GC_PERCENT=45.000 PRIMER_RIGHT_6_GC_PERCENT=42.857 PRIMER_LEFT_6_SELF_ANY=3.00 PRIMER_RIGHT_6_SELF_ANY=4.00 PRIMER_LEFT_6_SELF_END=3.00 PRIMER_RIGHT_6_SELF_END=1.00 PRIMER_LEFT_6_POSITION_PENALTY=0.000000 PRIMER_RIGHT_6_POSITION_PENALTY=1.000000 PRIMER_LEFT_6_END_STABILITY=10.0000 PRIMER_RIGHT_6_END_STABILITY=6.3000 PRIMER_PAIR_6_COMPL_ANY=5.00 PRIMER_PAIR_6_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_6=1086 PRIMER_PAIR_PENALTY_7=6.1128 PRIMER_LEFT_7_PENALTY=2.598622 PRIMER_RIGHT_7_PENALTY=3.514144 PRIMER_LEFT_7_SEQUENCE=ATGGACAAGTTTTGGTGGC PRIMER_RIGHT_7_SEQUENCE=ACACCCCAATCTTCATGTCCA PRIMER_LEFT_7=28,19 PRIMER_RIGHT_7=1111,21 PRIMER_LEFT_7_TM=58.401 PRIMER_RIGHT_7_TM=62.514 PRIMER_LEFT_7_GC_PERCENT=47.368 PRIMER_RIGHT_7_GC_PERCENT=47.619 PRIMER_LEFT_7_SELF_ANY=3.00 PRIMER_RIGHT_7_SELF_ANY=4.00 PRIMER_LEFT_7_SELF_END=2.00 PRIMER_RIGHT_7_SELF_END=2.00 PRIMER_LEFT_7_POSITION_PENALTY=0.000000 PRIMER_RIGHT_7_POSITION_PENALTY=0.000000 PRIMER_LEFT_7_END_STABILITY=9.4000 PRIMER_RIGHT_7_END_STABILITY=7.9000 PRIMER_PAIR_7_COMPL_ANY=6.00 PRIMER_PAIR_7_COMPL_END=3.00 PRIMER_PRODUCT_SIZE_7=1084 PRIMER_PAIR_PENALTY_8=6.1174 PRIMER_LEFT_8_PENALTY=1.335843 PRIMER_RIGHT_8_PENALTY=4.781508 PRIMER_LEFT_8_SEQUENCE=ATGGACAAGTTTTGGTGGCA PRIMER_RIGHT_8_SEQUENCE=TGTTACACCCCAATCTTCATGT PRIMER_LEFT_8=28,20 PRIMER_RIGHT_8=1115,22 PRIMER_LEFT_8_TM=61.336 PRIMER_RIGHT_8_TM=59.218 PRIMER_LEFT_8_GC_PERCENT=45.000 PRIMER_RIGHT_8_GC_PERCENT=40.909 PRIMER_LEFT_8_SELF_ANY=3.00 PRIMER_RIGHT_8_SELF_ANY=5.00 PRIMER_LEFT_8_SELF_END=3.00 PRIMER_RIGHT_8_SELF_END=2.00 PRIMER_LEFT_8_POSITION_PENALTY=0.000000 PRIMER_RIGHT_8_POSITION_PENALTY=2.000000 PRIMER_LEFT_8_END_STABILITY=10.0000 PRIMER_RIGHT_8_END_STABILITY=6.6000 PRIMER_PAIR_8_COMPL_ANY=5.00 PRIMER_PAIR_8_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_8=1088 PRIMER_PAIR_PENALTY_9=6.2650 PRIMER_LEFT_9_PENALTY=1.335843 PRIMER_RIGHT_9_PENALTY=4.929154 PRIMER_LEFT_9_SEQUENCE=ATGGACAAGTTTTGGTGGCA PRIMER_RIGHT_9_SEQUENCE=TACACCCCAATCTTCATGTCCA PRIMER_LEFT_9=28,20 PRIMER_RIGHT_9=1112,22 PRIMER_LEFT_9_TM=61.336 PRIMER_RIGHT_9_TM=62.429 PRIMER_LEFT_9_GC_PERCENT=45.000 PRIMER_RIGHT_9_GC_PERCENT=45.455 PRIMER_LEFT_9_SELF_ANY=3.00 PRIMER_RIGHT_9_SELF_ANY=4.00 PRIMER_LEFT_9_SELF_END=3.00 PRIMER_RIGHT_9_SELF_END=2.00 PRIMER_LEFT_9_POSITION_PENALTY=0.000000 PRIMER_RIGHT_9_POSITION_PENALTY=0.500000 PRIMER_LEFT_9_END_STABILITY=10.0000 PRIMER_RIGHT_9_END_STABILITY=7.9000 PRIMER_PAIR_9_COMPL_ANY=6.00 PRIMER_PAIR_9_COMPL_END=3.00 PRIMER_PRODUCT_SIZE_9=1085 PRIMER_PAIR_PENALTY_10=6.5429 PRIMER_LEFT_10_PENALTY=1.335843 PRIMER_RIGHT_10_PENALTY=5.207042 PRIMER_LEFT_10_SEQUENCE=ATGGACAAGTTTTGGTGGCA PRIMER_RIGHT_10_SEQUENCE=GTTACACCCCAATCTTCATGTC PRIMER_LEFT_10=28,20 PRIMER_RIGHT_10=1114,22 PRIMER_LEFT_10_TM=61.336 PRIMER_RIGHT_10_TM=58.293 PRIMER_LEFT_10_GC_PERCENT=45.000 PRIMER_RIGHT_10_GC_PERCENT=45.455 PRIMER_LEFT_10_SELF_ANY=3.00 PRIMER_RIGHT_10_SELF_ANY=4.00 PRIMER_LEFT_10_SELF_END=3.00 PRIMER_RIGHT_10_SELF_END=1.00 PRIMER_LEFT_10_POSITION_PENALTY=0.000000 PRIMER_RIGHT_10_POSITION_PENALTY=1.500000 PRIMER_LEFT_10_END_STABILITY=10.0000 PRIMER_RIGHT_10_END_STABILITY=6.3000 PRIMER_PAIR_10_COMPL_ANY=5.00 PRIMER_PAIR_10_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_10=1087 PRIMER_PAIR_PENALTY_11=6.5665 PRIMER_LEFT_11_PENALTY=2.598622 PRIMER_RIGHT_11_PENALTY=3.967916 PRIMER_LEFT_11_SEQUENCE=ATGGACAAGTTTTGGTGGC PRIMER_RIGHT_11_SEQUENCE=TTACACCCCAATCTTCATGTCC PRIMER_LEFT_11=28,19 PRIMER_RIGHT_11=1113,22 PRIMER_LEFT_11_TM=58.401 PRIMER_RIGHT_11_TM=60.968 PRIMER_LEFT_11_GC_PERCENT=47.368 PRIMER_RIGHT_11_GC_PERCENT=45.455 PRIMER_LEFT_11_SELF_ANY=3.00 PRIMER_RIGHT_11_SELF_ANY=4.00 PRIMER_LEFT_11_SELF_END=2.00 PRIMER_RIGHT_11_SELF_END=0.00 PRIMER_LEFT_11_POSITION_PENALTY=0.000000 PRIMER_RIGHT_11_POSITION_PENALTY=1.000000 PRIMER_LEFT_11_END_STABILITY=9.4000 PRIMER_RIGHT_11_END_STABILITY=7.9000 PRIMER_PAIR_11_COMPL_ANY=5.00 PRIMER_PAIR_11_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_11=1086 PRIMER_PAIR_PENALTY_12=6.8686 PRIMER_LEFT_12_PENALTY=1.335843 PRIMER_RIGHT_12_PENALTY=5.532744 PRIMER_LEFT_12_SEQUENCE=ATGGACAAGTTTTGGTGGCA PRIMER_RIGHT_12_SEQUENCE=GTGTTACACCCCAATCTTCATGT PRIMER_LEFT_12=28,20 PRIMER_RIGHT_12=1116,23 PRIMER_LEFT_12_TM=61.336 PRIMER_RIGHT_12_TM=60.033 PRIMER_LEFT_12_GC_PERCENT=45.000 PRIMER_RIGHT_12_GC_PERCENT=43.478 PRIMER_LEFT_12_SELF_ANY=3.00 PRIMER_RIGHT_12_SELF_ANY=7.00 PRIMER_LEFT_12_SELF_END=3.00 PRIMER_RIGHT_12_SELF_END=2.00 PRIMER_LEFT_12_POSITION_PENALTY=0.000000 PRIMER_RIGHT_12_POSITION_PENALTY=2.500000 PRIMER_LEFT_12_END_STABILITY=10.0000 PRIMER_RIGHT_12_END_STABILITY=6.6000 PRIMER_PAIR_12_COMPL_ANY=5.00 PRIMER_PAIR_12_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_12=1089 PRIMER_PAIR_PENALTY_13=7.2219 PRIMER_LEFT_13_PENALTY=2.598622 PRIMER_RIGHT_13_PENALTY=4.623327 PRIMER_LEFT_13_SEQUENCE=ATGGACAAGTTTTGGTGGC PRIMER_RIGHT_13_SEQUENCE=TTACACCCCAATCTTCATGTC PRIMER_LEFT_13=28,19 PRIMER_RIGHT_13=1113,21 PRIMER_LEFT_13_TM=58.401 PRIMER_RIGHT_13_TM=57.377 PRIMER_LEFT_13_GC_PERCENT=47.368 PRIMER_RIGHT_13_GC_PERCENT=42.857 PRIMER_LEFT_13_SELF_ANY=3.00 PRIMER_RIGHT_13_SELF_ANY=4.00 PRIMER_LEFT_13_SELF_END=2.00 PRIMER_RIGHT_13_SELF_END=1.00 PRIMER_LEFT_13_POSITION_PENALTY=0.000000 PRIMER_RIGHT_13_POSITION_PENALTY=1.000000 PRIMER_LEFT_13_END_STABILITY=9.4000 PRIMER_RIGHT_13_END_STABILITY=6.3000 PRIMER_PAIR_13_COMPL_ANY=5.00 PRIMER_PAIR_13_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_13=1086 PRIMER_PAIR_PENALTY_14=7.3461 PRIMER_LEFT_14_PENALTY=1.335843 PRIMER_RIGHT_14_PENALTY=6.010288 PRIMER_LEFT_14_SEQUENCE=ATGGACAAGTTTTGGTGGCA PRIMER_RIGHT_14_SEQUENCE=TGTTACACCCCAATCTTCATGTC PRIMER_LEFT_14=28,20 PRIMER_RIGHT_14=1115,23 PRIMER_LEFT_14_TM=61.336 PRIMER_RIGHT_14_TM=61.010 PRIMER_LEFT_14_GC_PERCENT=45.000 PRIMER_RIGHT_14_GC_PERCENT=43.478 PRIMER_LEFT_14_SELF_ANY=3.00 PRIMER_RIGHT_14_SELF_ANY=5.00 PRIMER_LEFT_14_SELF_END=3.00 PRIMER_RIGHT_14_SELF_END=1.00 PRIMER_LEFT_14_POSITION_PENALTY=0.000000 PRIMER_RIGHT_14_POSITION_PENALTY=2.000000 PRIMER_LEFT_14_END_STABILITY=10.0000 PRIMER_RIGHT_14_END_STABILITY=6.3000 PRIMER_PAIR_14_COMPL_ANY=5.00 PRIMER_PAIR_14_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_14=1088 PRIMER_PAIR_PENALTY_15=7.3801 PRIMER_LEFT_15_PENALTY=2.598622 PRIMER_RIGHT_15_PENALTY=4.781508 PRIMER_LEFT_15_SEQUENCE=ATGGACAAGTTTTGGTGGC PRIMER_RIGHT_15_SEQUENCE=TGTTACACCCCAATCTTCATGT PRIMER_LEFT_15=28,19 PRIMER_RIGHT_15=1115,22 PRIMER_LEFT_15_TM=58.401 PRIMER_RIGHT_15_TM=59.218 PRIMER_LEFT_15_GC_PERCENT=47.368 PRIMER_RIGHT_15_GC_PERCENT=40.909 PRIMER_LEFT_15_SELF_ANY=3.00 PRIMER_RIGHT_15_SELF_ANY=5.00 PRIMER_LEFT_15_SELF_END=2.00 PRIMER_RIGHT_15_SELF_END=2.00 PRIMER_LEFT_15_POSITION_PENALTY=0.000000 PRIMER_RIGHT_15_POSITION_PENALTY=2.000000 PRIMER_LEFT_15_END_STABILITY=9.4000 PRIMER_RIGHT_15_END_STABILITY=6.6000 PRIMER_PAIR_15_COMPL_ANY=5.00 PRIMER_PAIR_15_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_15=1088 PRIMER_PAIR_PENALTY_16=7.5278 PRIMER_LEFT_16_PENALTY=2.598622 PRIMER_RIGHT_16_PENALTY=4.929154 PRIMER_LEFT_16_SEQUENCE=ATGGACAAGTTTTGGTGGC PRIMER_RIGHT_16_SEQUENCE=TACACCCCAATCTTCATGTCCA PRIMER_LEFT_16=28,19 PRIMER_RIGHT_16=1112,22 PRIMER_LEFT_16_TM=58.401 PRIMER_RIGHT_16_TM=62.429 PRIMER_LEFT_16_GC_PERCENT=47.368 PRIMER_RIGHT_16_GC_PERCENT=45.455 PRIMER_LEFT_16_SELF_ANY=3.00 PRIMER_RIGHT_16_SELF_ANY=4.00 PRIMER_LEFT_16_SELF_END=2.00 PRIMER_RIGHT_16_SELF_END=2.00 PRIMER_LEFT_16_POSITION_PENALTY=0.000000 PRIMER_RIGHT_16_POSITION_PENALTY=0.500000 PRIMER_LEFT_16_END_STABILITY=9.4000 PRIMER_RIGHT_16_END_STABILITY=7.9000 PRIMER_PAIR_16_COMPL_ANY=6.00 PRIMER_PAIR_16_COMPL_END=3.00 PRIMER_PRODUCT_SIZE_16=1085 PRIMER_PAIR_PENALTY_17=7.5368 PRIMER_LEFT_17_PENALTY=1.335843 PRIMER_RIGHT_17_PENALTY=6.200997 PRIMER_LEFT_17_SEQUENCE=ATGGACAAGTTTTGGTGGCA PRIMER_RIGHT_17_SEQUENCE=GTTACACCCCAATCTTCATGTCC PRIMER_LEFT_17=28,20 PRIMER_RIGHT_17=1114,23 PRIMER_LEFT_17_TM=61.336 PRIMER_RIGHT_17_TM=61.701 PRIMER_LEFT_17_GC_PERCENT=45.000 PRIMER_RIGHT_17_GC_PERCENT=47.826 PRIMER_LEFT_17_SELF_ANY=3.00 PRIMER_RIGHT_17_SELF_ANY=4.00 PRIMER_LEFT_17_SELF_END=3.00 PRIMER_RIGHT_17_SELF_END=0.00 PRIMER_LEFT_17_POSITION_PENALTY=0.000000 PRIMER_RIGHT_17_POSITION_PENALTY=1.500000 PRIMER_LEFT_17_END_STABILITY=10.0000 PRIMER_RIGHT_17_END_STABILITY=7.9000 PRIMER_PAIR_17_COMPL_ANY=5.00 PRIMER_PAIR_17_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_17=1087 PRIMER_PAIR_PENALTY_18=7.8057 PRIMER_LEFT_18_PENALTY=2.598622 PRIMER_RIGHT_18_PENALTY=5.207042 PRIMER_LEFT_18_SEQUENCE=ATGGACAAGTTTTGGTGGC PRIMER_RIGHT_18_SEQUENCE=GTTACACCCCAATCTTCATGTC PRIMER_LEFT_18=28,19 PRIMER_RIGHT_18=1114,22 PRIMER_LEFT_18_TM=58.401 PRIMER_RIGHT_18_TM=58.293 PRIMER_LEFT_18_GC_PERCENT=47.368 PRIMER_RIGHT_18_GC_PERCENT=45.455 PRIMER_LEFT_18_SELF_ANY=3.00 PRIMER_RIGHT_18_SELF_ANY=4.00 PRIMER_LEFT_18_SELF_END=2.00 PRIMER_RIGHT_18_SELF_END=1.00 PRIMER_LEFT_18_POSITION_PENALTY=0.000000 PRIMER_RIGHT_18_POSITION_PENALTY=1.500000 PRIMER_LEFT_18_END_STABILITY=9.4000 PRIMER_RIGHT_18_END_STABILITY=6.3000 PRIMER_PAIR_18_COMPL_ANY=5.00 PRIMER_PAIR_18_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_18=1087 PRIMER_PAIR_PENALTY_19=7.8318 PRIMER_LEFT_19_PENALTY=2.598622 PRIMER_RIGHT_19_PENALTY=5.233212 PRIMER_LEFT_19_SEQUENCE=ATGGACAAGTTTTGGTGGC PRIMER_RIGHT_19_SEQUENCE=ACACCCCAATCTTCATGTCCAC PRIMER_LEFT_19=28,19 PRIMER_RIGHT_19=1111,22 PRIMER_LEFT_19_TM=58.401 PRIMER_RIGHT_19_TM=63.233 PRIMER_LEFT_19_GC_PERCENT=47.368 PRIMER_RIGHT_19_GC_PERCENT=50.000 PRIMER_LEFT_19_SELF_ANY=3.00 PRIMER_RIGHT_19_SELF_ANY=4.00 PRIMER_LEFT_19_SELF_END=2.00 PRIMER_RIGHT_19_SELF_END=2.00 PRIMER_LEFT_19_POSITION_PENALTY=0.000000 PRIMER_RIGHT_19_POSITION_PENALTY=0.000000 PRIMER_LEFT_19_END_STABILITY=9.4000 PRIMER_RIGHT_19_END_STABILITY=7.9000 PRIMER_PAIR_19_COMPL_ANY=6.00 PRIMER_PAIR_19_COMPL_END=3.00 PRIMER_PRODUCT_SIZE_19=1084 = PRIMER_SEQUENCE_ID=M67454/FAS PRIMER_COMMENT=Human Fas antigen (fas) mRNA, complete cds. CDS=195..1202 PRIMER_START_CODON_POSITION=194 SEQUENCE=GACGCTTCTGGGGAGTGAGGGAAGCGGTTTACGAGTGACTTGGCTGGAGCCTCAGGGGCGGGCACTGGCACGGAACACACCCTGAGGCCAGCCCTGGCTGCCCAGGCGGAGCTGCCTCTTCTCCCGCGGGTTGGTGGACCCGCTCAGTACGGAGTTGGGGAAGCTCTTTCACTTCGGAGGATTGCTCAACAACCATGCTGGGCATCTGGACCCTCCTACCTCTGGTTCTTACGTCTGTTGCTAGATTATCGTCCAAAAGTGTTAATGCCCAAGTGACTGACATCAACTCCAAGGGATTGGAATTGAGGAAGACTGTTACTACAGTTGAGACTCAGAACTTGGAAGGCCTGCATCATGATGGCCAATTCTGCCATAAGCCCTGTCCTCCAGGTGAAAGGAAAGCTAGGGACTGCACAGTCAATGGGGATGAACCAGACTGCGTGCCCTGCCAAGAAGGGAAGGAGTACACAGACAAAGCCCATTTTTCTTCCAAATGCAGAAGATGTAGATTGTGTGATGAAGGACATGGCTTAGAAGTGGAAATAAACTGCACCCGGACCCAGAATACCAAGTGCAGATGTAAACCAAACTTTTTTTGTAACTCTACTGTATGTGAACACTGTGACCCTTGCACCAAATGTGAACATGGAATCATCAAGGAATGCACACTCACCAGCAACACCAAGTGCAAAGAGGAAGGATCCAGATCTAACTTGGGGTGGCTTTGTCTTCTTCTTTTGCCAATTCCACTAATTGTTTGGGTGAAGAGAAAGGAAGTACAGAAAACATGCAGAAAGCACAGAAAGGAAAACCAAGGTTCTCATGAATCTCCAACCTTAAATCCTGAAACAGTGGCAATAAATTTATCTGATGTTGACTTGAGTAAATATATCACCACTATTGCTGGAGTCATGACACTAAGTCAAGTTAAAGGCTTTGTTCGAAAGAATGGTGTCAATGAAGCCAAAATAGATGAGATCAAGAATGACAATGTCCAAGACACAGCAGAACAGAAAGTTCAACTGCTTCGTAATTGGCATCAACTTCATGGAAAGAAAGAAGCGTATGACACATTGATTAAAGATCTCAAAAAAGCCAATCTTTGTACTCTTGCAGAGAAAATTCAGACTATCATCCTCAAGGACATTACTAGTGACTCAGAAAATTCAAACTTCAGAAATGAAATCCAAAGCTTGGTCTAGAGTGAAAAACAACAAATTCAGTTCTGAGTATATGCAATTAGTGTTTGAAAAGATTCTTAATAGCTGGCTGTAAATACTGCTTGGTTTTTTACTGGGTACATTTTATCATTTATTAGCGCTGAAGAGCCAACATATTTGTAGATTTTTAATATCTCATGATTCTGCCTCCAAGGATGTTTAAAATCTAGTTGGGAAAACAAACTTCATCAAGAGTAAATGCAGTGGCATGCTAAGTACCCAAATAGGAGTGTATGCAGAGGATGAAAGATTAAGATTATGCTCTGGCATCTAACATATGATTCTGTAGTATGAATGTAATCAGTGTATGTTAGTACAAATGTCTATCCACAGGCTAACCCCACTCTATGAATCAATAGAAGAAGCTATGACCTTTTGCTGAAATATCAGTTACTGAACAGGCAGGCCACTTTGCCTCTAAATTACCTCTGATAATTCTAGAGATTTTACCATATTTCTAAACTTTGTTTATAACTCTGAGAAGATCATATTTATGTAAAGTATATGTATTTGAGTGCAGAATTTAAATAAGGCTCTACCTCAAAGACCTTTGCACAGTTTATTGGTGTCATATTATACAATATTTCAATTGTGAATTCACATAGAAAACATTAAATTATAATGTTTGACTATTATATATGTGTATGCATTTTACTGGCTCAAAACTACCTACTTCTTTCTCAGGCATCAAAAGCATTTTGAGCAGGAGAGTATTACTAGAGCTTTGCCACCTCTCCATTTTTGCCTTGGTGCTCATCTTAATGGCCTAATGCACCCCCAAACATGGAAATATCACCAAAAAATACTTAATAGTCCACCAAAAGGCAAGACTGCCCTTAGAAATTCTAGCCTGGTTTGGAGATACTAACTGCTCTCAGAGAAAGTAGCTTTGTGACATGTCATGAACCCATGTTTGCAATCAAAGATGATAAAATAGATTCTTATTTTTCCCCCACCCCCGAAAATGTTCAATAATGTCCCATGTAAAACCTGCTACAAATGGCAGCTTATACATAGCAATGGTAAAATCATCATCTGGATTTAGGAATTGCTCTTGTCATACCCTCAAGTTTCTAAGATTTAAGATTCTCCTTACTACTATCCTACGTTTAAATATCTTTGAAAGTTTGTATTAAATGTGAATTTTAAGAAATAATATTTATATTTCTGTAAATGTAAACTGTGAAGATAGTTATAAACTGAAGCAGATACCTGGAACCACCTAAAGAACTTCCATTTATGGAGGATTTTTTTGCCCCTTGTGTTTGGAATTATAAAATATAGGTAAAAGTACGTAATTAAATAATGTTTTTG PRIMER_LEFT_EXPLAIN=considered 21749, would not amplify any of the ORF 18372, GC content failed 10, low tm 984, high tm 1002, long poly-x seq 44, high 3' stability 21, ok 1316 PRIMER_RIGHT_EXPLAIN=considered 21076, GC content failed 1260, low tm 8619, high tm 3776, long poly-x seq 112, high 3' stability 48, ok 7261 PRIMER_PAIR_EXPLAIN=considered 21, ok 21 PRIMER_STOP_CODON_POSITION=1199 PRIMER_PAIR_PENALTY=124.1313 PRIMER_LEFT_PENALTY=121.524367 PRIMER_RIGHT_PENALTY=2.606973 PRIMER_LEFT_SEQUENCE=GGCATCTGGACCCTCCTAC PRIMER_RIGHT_SEQUENCE=AGACCAAGCTTTGGATTTCA PRIMER_LEFT=200,19 PRIMER_RIGHT=1199,20 PRIMER_LEFT_TM=59.476 PRIMER_RIGHT_TM=57.393 PRIMER_LEFT_GC_PERCENT=63.158 PRIMER_RIGHT_GC_PERCENT=40.000 PRIMER_LEFT_SELF_ANY=3.00 PRIMER_RIGHT_SELF_ANY=6.00 PRIMER_LEFT_SELF_END=0.00 PRIMER_RIGHT_SELF_END=3.00 PRIMER_LEFT_POSITION_PENALTY=120.000000 PRIMER_RIGHT_POSITION_PENALTY=0.000000 PRIMER_LEFT_END_STABILITY=6.9000 PRIMER_RIGHT_END_STABILITY=7.3000 PRIMER_PAIR_COMPL_ANY=3.00 PRIMER_PAIR_COMPL_END=0.00 PRIMER_PRODUCT_SIZE=1000 PRIMER_PAIR_PENALTY_1=124.3881 PRIMER_LEFT_1_PENALTY=121.524367 PRIMER_RIGHT_1_PENALTY=2.863756 PRIMER_LEFT_1_SEQUENCE=GGCATCTGGACCCTCCTAC PRIMER_RIGHT_1_SEQUENCE=AGACCAAGCTTTGGATTTCATT PRIMER_LEFT_1=200,19 PRIMER_RIGHT_1=1199,22 PRIMER_LEFT_1_TM=59.476 PRIMER_RIGHT_1_TM=59.136 PRIMER_LEFT_1_GC_PERCENT=63.158 PRIMER_RIGHT_1_GC_PERCENT=36.364 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=1.00 PRIMER_LEFT_1_POSITION_PENALTY=120.000000 PRIMER_RIGHT_1_POSITION_PENALTY=0.000000 PRIMER_LEFT_1_END_STABILITY=6.9000 PRIMER_RIGHT_1_END_STABILITY=6.9000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_1=1000 PRIMER_PAIR_PENALTY_2=124.7260 PRIMER_LEFT_2_PENALTY=121.524367 PRIMER_RIGHT_2_PENALTY=3.201621 PRIMER_LEFT_2_SEQUENCE=GGCATCTGGACCCTCCTAC PRIMER_RIGHT_2_SEQUENCE=AGACCAAGCTTTGGATTTCAT PRIMER_LEFT_2=200,19 PRIMER_RIGHT_2=1199,21 PRIMER_LEFT_2_TM=59.476 PRIMER_RIGHT_2_TM=57.798 PRIMER_LEFT_2_GC_PERCENT=63.158 PRIMER_RIGHT_2_GC_PERCENT=38.095 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_LEFT_2_POSITION_PENALTY=120.000000 PRIMER_RIGHT_2_POSITION_PENALTY=0.000000 PRIMER_LEFT_2_END_STABILITY=6.9000 PRIMER_RIGHT_2_END_STABILITY=6.9000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_2=1000 PRIMER_PAIR_PENALTY_3=124.8704 PRIMER_LEFT_3_PENALTY=121.524367 PRIMER_RIGHT_3_PENALTY=3.346072 PRIMER_LEFT_3_SEQUENCE=GGCATCTGGACCCTCCTAC PRIMER_RIGHT_3_SEQUENCE=AGACCAAGCTTTGGATTTCATTT PRIMER_LEFT_3=200,19 PRIMER_RIGHT_3=1199,23 PRIMER_LEFT_3_TM=59.476 PRIMER_RIGHT_3_TM=60.346 PRIMER_LEFT_3_GC_PERCENT=63.158 PRIMER_RIGHT_3_GC_PERCENT=34.783 PRIMER_LEFT_3_SELF_ANY=3.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_LEFT_3_POSITION_PENALTY=120.000000 PRIMER_RIGHT_3_POSITION_PENALTY=0.000000 PRIMER_LEFT_3_END_STABILITY=6.9000 PRIMER_RIGHT_3_END_STABILITY=7.2000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_3=1000 PRIMER_PAIR_PENALTY_4=125.5220 PRIMER_LEFT_4_PENALTY=121.524367 PRIMER_RIGHT_4_PENALTY=3.997622 PRIMER_LEFT_4_SEQUENCE=GGCATCTGGACCCTCCTAC PRIMER_RIGHT_4_SEQUENCE=TAGACCAAGCTTTGGATTTCA PRIMER_LEFT_4=200,19 PRIMER_RIGHT_4=1200,21 PRIMER_LEFT_4_TM=59.476 PRIMER_RIGHT_4_TM=57.502 PRIMER_LEFT_4_GC_PERCENT=63.158 PRIMER_RIGHT_4_GC_PERCENT=38.095 PRIMER_LEFT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=3.00 PRIMER_LEFT_4_POSITION_PENALTY=120.000000 PRIMER_RIGHT_4_POSITION_PENALTY=0.500000 PRIMER_LEFT_4_END_STABILITY=6.9000 PRIMER_RIGHT_4_END_STABILITY=7.3000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_4=1001 PRIMER_PAIR_PENALTY_5=125.6899 PRIMER_LEFT_5_PENALTY=123.082915 PRIMER_RIGHT_5_PENALTY=2.606973 PRIMER_LEFT_5_SEQUENCE=GGCATCTGGACCCTCCTACC PRIMER_RIGHT_5_SEQUENCE=AGACCAAGCTTTGGATTTCA PRIMER_LEFT_5=200,20 PRIMER_RIGHT_5=1199,20 PRIMER_LEFT_5_TM=63.083 PRIMER_RIGHT_5_TM=57.393 PRIMER_LEFT_5_GC_PERCENT=65.000 PRIMER_RIGHT_5_GC_PERCENT=40.000 PRIMER_LEFT_5_SELF_ANY=3.00 PRIMER_RIGHT_5_SELF_ANY=6.00 PRIMER_LEFT_5_SELF_END=0.00 PRIMER_RIGHT_5_SELF_END=3.00 PRIMER_LEFT_5_POSITION_PENALTY=120.000000 PRIMER_RIGHT_5_POSITION_PENALTY=0.000000 PRIMER_LEFT_5_END_STABILITY=6.9000 PRIMER_RIGHT_5_END_STABILITY=7.3000 PRIMER_PAIR_5_COMPL_ANY=3.00 PRIMER_PAIR_5_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_5=1000 PRIMER_PAIR_PENALTY_6=125.8489 PRIMER_LEFT_6_PENALTY=121.524367 PRIMER_RIGHT_6_PENALTY=4.324579 PRIMER_LEFT_6_SEQUENCE=GGCATCTGGACCCTCCTAC PRIMER_RIGHT_6_SEQUENCE=TAGACCAAGCTTTGGATTTCATT PRIMER_LEFT_6=200,19 PRIMER_RIGHT_6=1200,23 PRIMER_LEFT_6_TM=59.476 PRIMER_RIGHT_6_TM=59.175 PRIMER_LEFT_6_GC_PERCENT=63.158 PRIMER_RIGHT_6_GC_PERCENT=34.783 PRIMER_LEFT_6_SELF_ANY=3.00 PRIMER_RIGHT_6_SELF_ANY=6.00 PRIMER_LEFT_6_SELF_END=0.00 PRIMER_RIGHT_6_SELF_END=1.00 PRIMER_LEFT_6_POSITION_PENALTY=120.000000 PRIMER_RIGHT_6_POSITION_PENALTY=0.500000 PRIMER_LEFT_6_END_STABILITY=6.9000 PRIMER_RIGHT_6_END_STABILITY=6.9000 PRIMER_PAIR_6_COMPL_ANY=3.00 PRIMER_PAIR_6_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_6=1001 PRIMER_PAIR_PENALTY_7=125.9467 PRIMER_LEFT_7_PENALTY=123.082915 PRIMER_RIGHT_7_PENALTY=2.863756 PRIMER_LEFT_7_SEQUENCE=GGCATCTGGACCCTCCTACC PRIMER_RIGHT_7_SEQUENCE=AGACCAAGCTTTGGATTTCATT PRIMER_LEFT_7=200,20 PRIMER_RIGHT_7=1199,22 PRIMER_LEFT_7_TM=63.083 PRIMER_RIGHT_7_TM=59.136 PRIMER_LEFT_7_GC_PERCENT=65.000 PRIMER_RIGHT_7_GC_PERCENT=36.364 PRIMER_LEFT_7_SELF_ANY=3.00 PRIMER_RIGHT_7_SELF_ANY=6.00 PRIMER_LEFT_7_SELF_END=0.00 PRIMER_RIGHT_7_SELF_END=1.00 PRIMER_LEFT_7_POSITION_PENALTY=120.000000 PRIMER_RIGHT_7_POSITION_PENALTY=0.000000 PRIMER_LEFT_7_END_STABILITY=6.9000 PRIMER_RIGHT_7_END_STABILITY=6.9000 PRIMER_PAIR_7_COMPL_ANY=3.00 PRIMER_PAIR_7_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_7=1000 PRIMER_PAIR_PENALTY_8=126.0095 PRIMER_LEFT_8_PENALTY=121.524367 PRIMER_RIGHT_8_PENALTY=4.485099 PRIMER_LEFT_8_SEQUENCE=GGCATCTGGACCCTCCTAC PRIMER_RIGHT_8_SEQUENCE=CTAGACCAAGCTTTGGATTTCA PRIMER_LEFT_8=200,19 PRIMER_RIGHT_8=1201,22 PRIMER_LEFT_8_TM=59.476 PRIMER_RIGHT_8_TM=58.515 PRIMER_LEFT_8_GC_PERCENT=63.158 PRIMER_RIGHT_8_GC_PERCENT=40.909 PRIMER_LEFT_8_SELF_ANY=3.00 PRIMER_RIGHT_8_SELF_ANY=6.00 PRIMER_LEFT_8_SELF_END=0.00 PRIMER_RIGHT_8_SELF_END=3.00 PRIMER_LEFT_8_POSITION_PENALTY=120.000000 PRIMER_RIGHT_8_POSITION_PENALTY=1.000000 PRIMER_LEFT_8_END_STABILITY=6.9000 PRIMER_RIGHT_8_END_STABILITY=7.3000 PRIMER_PAIR_8_COMPL_ANY=3.00 PRIMER_PAIR_8_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_8=1002 PRIMER_PAIR_PENALTY_9=126.1368 PRIMER_LEFT_9_PENALTY=121.524367 PRIMER_RIGHT_9_PENALTY=4.612386 PRIMER_LEFT_9_SEQUENCE=GGCATCTGGACCCTCCTAC PRIMER_RIGHT_9_SEQUENCE=TAGACCAAGCTTTGGATTTCAT PRIMER_LEFT_9=200,19 PRIMER_RIGHT_9=1200,22 PRIMER_LEFT_9_TM=59.476 PRIMER_RIGHT_9_TM=57.888 PRIMER_LEFT_9_GC_PERCENT=63.158 PRIMER_RIGHT_9_GC_PERCENT=36.364 PRIMER_LEFT_9_SELF_ANY=3.00 PRIMER_RIGHT_9_SELF_ANY=6.00 PRIMER_LEFT_9_SELF_END=0.00 PRIMER_RIGHT_9_SELF_END=2.00 PRIMER_LEFT_9_POSITION_PENALTY=120.000000 PRIMER_RIGHT_9_POSITION_PENALTY=0.500000 PRIMER_LEFT_9_END_STABILITY=6.9000 PRIMER_RIGHT_9_END_STABILITY=6.9000 PRIMER_PAIR_9_COMPL_ANY=3.00 PRIMER_PAIR_9_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_9=1001 PRIMER_PAIR_PENALTY_10=126.2673 PRIMER_LEFT_10_PENALTY=121.524367 PRIMER_RIGHT_10_PENALTY=4.742983 PRIMER_LEFT_10_SEQUENCE=GGCATCTGGACCCTCCTAC PRIMER_RIGHT_10_SEQUENCE=TCTAGACCAAGCTTTGGATTTCA PRIMER_LEFT_10=200,19 PRIMER_RIGHT_10=1202,23 PRIMER_LEFT_10_TM=59.476 PRIMER_RIGHT_10_TM=60.243 PRIMER_LEFT_10_GC_PERCENT=63.158 PRIMER_RIGHT_10_GC_PERCENT=39.130 PRIMER_LEFT_10_SELF_ANY=3.00 PRIMER_RIGHT_10_SELF_ANY=6.00 PRIMER_LEFT_10_SELF_END=0.00 PRIMER_RIGHT_10_SELF_END=3.00 PRIMER_LEFT_10_POSITION_PENALTY=120.000000 PRIMER_RIGHT_10_POSITION_PENALTY=1.500000 PRIMER_LEFT_10_END_STABILITY=6.9000 PRIMER_RIGHT_10_END_STABILITY=7.3000 PRIMER_PAIR_10_COMPL_ANY=4.00 PRIMER_PAIR_10_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_10=1003 PRIMER_PAIR_PENALTY_11=126.2845 PRIMER_LEFT_11_PENALTY=123.082915 PRIMER_RIGHT_11_PENALTY=3.201621 PRIMER_LEFT_11_SEQUENCE=GGCATCTGGACCCTCCTACC PRIMER_RIGHT_11_SEQUENCE=AGACCAAGCTTTGGATTTCAT PRIMER_LEFT_11=200,20 PRIMER_RIGHT_11=1199,21 PRIMER_LEFT_11_TM=63.083 PRIMER_RIGHT_11_TM=57.798 PRIMER_LEFT_11_GC_PERCENT=65.000 PRIMER_RIGHT_11_GC_PERCENT=38.095 PRIMER_LEFT_11_SELF_ANY=3.00 PRIMER_RIGHT_11_SELF_ANY=6.00 PRIMER_LEFT_11_SELF_END=0.00 PRIMER_RIGHT_11_SELF_END=2.00 PRIMER_LEFT_11_POSITION_PENALTY=120.000000 PRIMER_RIGHT_11_POSITION_PENALTY=0.000000 PRIMER_LEFT_11_END_STABILITY=6.9000 PRIMER_RIGHT_11_END_STABILITY=6.9000 PRIMER_PAIR_11_COMPL_ANY=3.00 PRIMER_PAIR_11_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_11=1000 PRIMER_PAIR_PENALTY_12=126.3684 PRIMER_LEFT_12_PENALTY=121.524367 PRIMER_RIGHT_12_PENALTY=4.843999 PRIMER_LEFT_12_SEQUENCE=GGCATCTGGACCCTCCTAC PRIMER_RIGHT_12_SEQUENCE=TAGACCAAGCTTTGGATTTCATTT PRIMER_LEFT_12=200,19 PRIMER_RIGHT_12=1200,24 PRIMER_LEFT_12_TM=59.476 PRIMER_RIGHT_12_TM=60.344 PRIMER_LEFT_12_GC_PERCENT=63.158 PRIMER_RIGHT_12_GC_PERCENT=33.333 PRIMER_LEFT_12_SELF_ANY=3.00 PRIMER_RIGHT_12_SELF_ANY=6.00 PRIMER_LEFT_12_SELF_END=0.00 PRIMER_RIGHT_12_SELF_END=0.00 PRIMER_LEFT_12_POSITION_PENALTY=120.000000 PRIMER_RIGHT_12_POSITION_PENALTY=0.500000 PRIMER_LEFT_12_END_STABILITY=6.9000 PRIMER_RIGHT_12_END_STABILITY=7.2000 PRIMER_PAIR_12_COMPL_ANY=3.00 PRIMER_PAIR_12_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_12=1001 PRIMER_PAIR_PENALTY_13=126.4290 PRIMER_LEFT_13_PENALTY=123.082915 PRIMER_RIGHT_13_PENALTY=3.346072 PRIMER_LEFT_13_SEQUENCE=GGCATCTGGACCCTCCTACC PRIMER_RIGHT_13_SEQUENCE=AGACCAAGCTTTGGATTTCATTT PRIMER_LEFT_13=200,20 PRIMER_RIGHT_13=1199,23 PRIMER_LEFT_13_TM=63.083 PRIMER_RIGHT_13_TM=60.346 PRIMER_LEFT_13_GC_PERCENT=65.000 PRIMER_RIGHT_13_GC_PERCENT=34.783 PRIMER_LEFT_13_SELF_ANY=3.00 PRIMER_RIGHT_13_SELF_ANY=6.00 PRIMER_LEFT_13_SELF_END=0.00 PRIMER_RIGHT_13_SELF_END=0.00 PRIMER_LEFT_13_POSITION_PENALTY=120.000000 PRIMER_RIGHT_13_POSITION_PENALTY=0.000000 PRIMER_LEFT_13_END_STABILITY=6.9000 PRIMER_RIGHT_13_END_STABILITY=7.2000 PRIMER_PAIR_13_COMPL_ANY=3.00 PRIMER_PAIR_13_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_13=1000 PRIMER_PAIR_PENALTY_14=126.5506 PRIMER_LEFT_14_PENALTY=121.524367 PRIMER_RIGHT_14_PENALTY=5.026278 PRIMER_LEFT_14_SEQUENCE=GGCATCTGGACCCTCCTAC PRIMER_RIGHT_14_SEQUENCE=CTAGACCAAGCTTTGGATTTCATT PRIMER_LEFT_14=200,19 PRIMER_RIGHT_14=1201,24 PRIMER_LEFT_14_TM=59.476 PRIMER_RIGHT_14_TM=60.026 PRIMER_LEFT_14_GC_PERCENT=63.158 PRIMER_RIGHT_14_GC_PERCENT=37.500 PRIMER_LEFT_14_SELF_ANY=3.00 PRIMER_RIGHT_14_SELF_ANY=6.00 PRIMER_LEFT_14_SELF_END=0.00 PRIMER_RIGHT_14_SELF_END=1.00 PRIMER_LEFT_14_POSITION_PENALTY=120.000000 PRIMER_RIGHT_14_POSITION_PENALTY=1.000000 PRIMER_LEFT_14_END_STABILITY=6.9000 PRIMER_RIGHT_14_END_STABILITY=6.9000 PRIMER_PAIR_14_COMPL_ANY=3.00 PRIMER_PAIR_14_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_14=1002 PRIMER_PAIR_PENALTY_15=126.6898 PRIMER_LEFT_15_PENALTY=121.524367 PRIMER_RIGHT_15_PENALTY=5.165434 PRIMER_LEFT_15_SEQUENCE=GGCATCTGGACCCTCCTAC PRIMER_RIGHT_15_SEQUENCE=CTAGACCAAGCTTTGGATTTCAT PRIMER_LEFT_15=200,19 PRIMER_RIGHT_15=1201,23 PRIMER_LEFT_15_TM=59.476 PRIMER_RIGHT_15_TM=58.835 PRIMER_LEFT_15_GC_PERCENT=63.158 PRIMER_RIGHT_15_GC_PERCENT=39.130 PRIMER_LEFT_15_SELF_ANY=3.00 PRIMER_RIGHT_15_SELF_ANY=6.00 PRIMER_LEFT_15_SELF_END=0.00 PRIMER_RIGHT_15_SELF_END=2.00 PRIMER_LEFT_15_POSITION_PENALTY=120.000000 PRIMER_RIGHT_15_POSITION_PENALTY=1.000000 PRIMER_LEFT_15_END_STABILITY=6.9000 PRIMER_RIGHT_15_END_STABILITY=6.9000 PRIMER_PAIR_15_COMPL_ANY=3.00 PRIMER_PAIR_15_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_15=1002 PRIMER_PAIR_PENALTY_16=127.0805 PRIMER_LEFT_16_PENALTY=123.082915 PRIMER_RIGHT_16_PENALTY=3.997622 PRIMER_LEFT_16_SEQUENCE=GGCATCTGGACCCTCCTACC PRIMER_RIGHT_16_SEQUENCE=TAGACCAAGCTTTGGATTTCA PRIMER_LEFT_16=200,20 PRIMER_RIGHT_16=1200,21 PRIMER_LEFT_16_TM=63.083 PRIMER_RIGHT_16_TM=57.502 PRIMER_LEFT_16_GC_PERCENT=65.000 PRIMER_RIGHT_16_GC_PERCENT=38.095 PRIMER_LEFT_16_SELF_ANY=3.00 PRIMER_RIGHT_16_SELF_ANY=6.00 PRIMER_LEFT_16_SELF_END=0.00 PRIMER_RIGHT_16_SELF_END=3.00 PRIMER_LEFT_16_POSITION_PENALTY=120.000000 PRIMER_RIGHT_16_POSITION_PENALTY=0.500000 PRIMER_LEFT_16_END_STABILITY=6.9000 PRIMER_RIGHT_16_END_STABILITY=7.3000 PRIMER_PAIR_16_COMPL_ANY=3.00 PRIMER_PAIR_16_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_16=1001 PRIMER_PAIR_PENALTY_17=127.3898 PRIMER_LEFT_17_PENALTY=121.524367 PRIMER_RIGHT_17_PENALTY=5.865428 PRIMER_LEFT_17_SEQUENCE=GGCATCTGGACCCTCCTAC PRIMER_RIGHT_17_SEQUENCE=TCTAGACCAAGCTTTGGATTTC PRIMER_LEFT_17=200,19 PRIMER_RIGHT_17=1202,22 PRIMER_LEFT_17_TM=59.476 PRIMER_RIGHT_17_TM=57.635 PRIMER_LEFT_17_GC_PERCENT=63.158 PRIMER_RIGHT_17_GC_PERCENT=40.909 PRIMER_LEFT_17_SELF_ANY=3.00 PRIMER_RIGHT_17_SELF_ANY=6.00 PRIMER_LEFT_17_SELF_END=0.00 PRIMER_RIGHT_17_SELF_END=2.00 PRIMER_LEFT_17_POSITION_PENALTY=120.000000 PRIMER_RIGHT_17_POSITION_PENALTY=1.500000 PRIMER_LEFT_17_END_STABILITY=6.9000 PRIMER_RIGHT_17_END_STABILITY=6.9000 PRIMER_PAIR_17_COMPL_ANY=4.00 PRIMER_PAIR_17_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_17=1003 PRIMER_PAIR_PENALTY_18=127.4075 PRIMER_LEFT_18_PENALTY=123.082915 PRIMER_RIGHT_18_PENALTY=4.324579 PRIMER_LEFT_18_SEQUENCE=GGCATCTGGACCCTCCTACC PRIMER_RIGHT_18_SEQUENCE=TAGACCAAGCTTTGGATTTCATT PRIMER_LEFT_18=200,20 PRIMER_RIGHT_18=1200,23 PRIMER_LEFT_18_TM=63.083 PRIMER_RIGHT_18_TM=59.175 PRIMER_LEFT_18_GC_PERCENT=65.000 PRIMER_RIGHT_18_GC_PERCENT=34.783 PRIMER_LEFT_18_SELF_ANY=3.00 PRIMER_RIGHT_18_SELF_ANY=6.00 PRIMER_LEFT_18_SELF_END=0.00 PRIMER_RIGHT_18_SELF_END=1.00 PRIMER_LEFT_18_POSITION_PENALTY=120.000000 PRIMER_RIGHT_18_POSITION_PENALTY=0.500000 PRIMER_LEFT_18_END_STABILITY=6.9000 PRIMER_RIGHT_18_END_STABILITY=6.9000 PRIMER_PAIR_18_COMPL_ANY=3.00 PRIMER_PAIR_18_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_18=1001 PRIMER_PAIR_PENALTY_19=127.4358 PRIMER_LEFT_19_PENALTY=121.524367 PRIMER_RIGHT_19_PENALTY=5.911413 PRIMER_LEFT_19_SEQUENCE=GGCATCTGGACCCTCCTAC PRIMER_RIGHT_19_SEQUENCE=AGACCAAGCTTTGGATTTCATTTC PRIMER_LEFT_19=200,19 PRIMER_RIGHT_19=1199,24 PRIMER_LEFT_19_TM=59.476 PRIMER_RIGHT_19_TM=61.911 PRIMER_LEFT_19_GC_PERCENT=63.158 PRIMER_RIGHT_19_GC_PERCENT=37.500 PRIMER_LEFT_19_SELF_ANY=3.00 PRIMER_RIGHT_19_SELF_ANY=6.00 PRIMER_LEFT_19_SELF_END=0.00 PRIMER_RIGHT_19_SELF_END=1.00 PRIMER_LEFT_19_POSITION_PENALTY=120.000000 PRIMER_RIGHT_19_POSITION_PENALTY=0.000000 PRIMER_LEFT_19_END_STABILITY=6.9000 PRIMER_RIGHT_19_END_STABILITY=6.9000 PRIMER_PAIR_19_COMPL_ANY=3.00 PRIMER_PAIR_19_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_19=1000 = PRIMER_SEQUENCE_ID=U33760/SKP1 PRIMER_COMMENT=Human cyclin A/CDK2-associated p19 (Skp1) mRNA, complete cds. CDS=94..585 PRIMER_START_CODON_POSITION=93 SEQUENCE=AGGATCCTCGAGGCCACGAAGGCCCTCTCCCTTCGCAAACGCCTCCCGGCTCTCGTAAGCCTCCCGCCGGCCGTCTCCTTAACACCGAACACCATGCCTTCAATTAAGTTGCAGAGTTCTGATGGAGAGATATTTGAAGTTGATGTGGAAATTGCCAAACAATCTGTAACTATTAAGACCATGTTGGAAGATTTGGGAATGGATGATGAAGGAGATGATGACCCAGTTCCTCTACCAAATGTGAATGCAGCAATATTAAAAAAGGTCATTCAGTGGTGCACCCACCACAAGGATGACCCTCCTCCTCCTGAAGATGATGAGAACAAAGAAAAGCGGACAGATGATATCCCTGTTTGGGACCAAGAATTCCTGAAAGTTGACCAAGGAACACTTTTTGAACTCATTCTGGCTGCAAACTACTTAGACATCAAAGGTTTGCTTGATGTTACATGCAAGACTGTTGCCAATATGATCAAGGGGAAAACTCCTGAGGAGATTCGCAAGACCTTCAATATCAAAAATGACTTTACTGAAGAGGAGGAAGCCCAGGTACGCAAAGAGAACCAGTGGTGTGAAGAGAAGTGAAATGTTGTGCCTGACACTGTAACACTGTAAGGAT PRIMER_LEFT_EXPLAIN=considered 4706, would not amplify any of the ORF 3123, GC content failed 5, low tm 396, high tm 523, high end compl 7, long poly-x seq 20, high 3' stability 13, ok 619 PRIMER_RIGHT_EXPLAIN=considered 4645, GC content failed 11, low tm 1320, high tm 1167, high end compl 10, long poly-x seq 22, high 3' stability 16, ok 2099 PRIMER_PAIR_EXPLAIN=considered 21, ok 21 PRIMER_STOP_CODON_POSITION=582 PRIMER_PAIR_PENALTY=12.3522 PRIMER_LEFT_PENALTY=9.051374 PRIMER_RIGHT_PENALTY=3.300825 PRIMER_LEFT_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC PRIMER_RIGHT_SEQUENCE=ACTTCTCTTCACACCACTGGTTC PRIMER_LEFT=93,26 PRIMER_RIGHT=582,23 PRIMER_LEFT_TM=63.051 PRIMER_RIGHT_TM=59.699 PRIMER_LEFT_GC_PERCENT=38.462 PRIMER_RIGHT_GC_PERCENT=47.826 PRIMER_LEFT_SELF_ANY=8.00 PRIMER_RIGHT_SELF_ANY=7.00 PRIMER_LEFT_SELF_END=2.00 PRIMER_RIGHT_SELF_END=3.00 PRIMER_LEFT_POSITION_PENALTY=0.000000 PRIMER_RIGHT_POSITION_PENALTY=0.000000 PRIMER_LEFT_END_STABILITY=6.4000 PRIMER_RIGHT_END_STABILITY=7.9000 PRIMER_PAIR_COMPL_ANY=5.00 PRIMER_PAIR_COMPL_END=0.00 PRIMER_PRODUCT_SIZE=490 PRIMER_PAIR_PENALTY_1=13.6635 PRIMER_LEFT_1_PENALTY=9.051374 PRIMER_RIGHT_1_PENALTY=4.612170 PRIMER_LEFT_1_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC PRIMER_RIGHT_1_SEQUENCE=ACTTCTCTTCACACCACTGGTTCT PRIMER_LEFT_1=93,26 PRIMER_RIGHT_1=582,24 PRIMER_LEFT_1_TM=63.051 PRIMER_RIGHT_1_TM=60.612 PRIMER_LEFT_1_GC_PERCENT=38.462 PRIMER_RIGHT_1_GC_PERCENT=45.833 PRIMER_LEFT_1_SELF_ANY=8.00 PRIMER_RIGHT_1_SELF_ANY=7.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_LEFT_1_POSITION_PENALTY=0.000000 PRIMER_RIGHT_1_POSITION_PENALTY=0.000000 PRIMER_LEFT_1_END_STABILITY=6.4000 PRIMER_RIGHT_1_END_STABILITY=6.4000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_1=490 PRIMER_PAIR_PENALTY_2=14.5623 PRIMER_LEFT_2_PENALTY=9.051374 PRIMER_RIGHT_2_PENALTY=5.510876 PRIMER_LEFT_2_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC PRIMER_RIGHT_2_SEQUENCE=TTCACTTCTCTTCACACCACTG PRIMER_LEFT_2=93,26 PRIMER_RIGHT_2=585,22 PRIMER_LEFT_2_TM=63.051 PRIMER_RIGHT_2_TM=57.989 PRIMER_LEFT_2_GC_PERCENT=38.462 PRIMER_RIGHT_2_GC_PERCENT=45.455 PRIMER_LEFT_2_SELF_ANY=8.00 PRIMER_RIGHT_2_SELF_ANY=3.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_LEFT_2_POSITION_PENALTY=0.000000 PRIMER_RIGHT_2_POSITION_PENALTY=1.500000 PRIMER_LEFT_2_END_STABILITY=6.4000 PRIMER_RIGHT_2_END_STABILITY=6.7000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_2=493 PRIMER_PAIR_PENALTY_3=14.6221 PRIMER_LEFT_3_PENALTY=9.051374 PRIMER_RIGHT_3_PENALTY=5.570716 PRIMER_LEFT_3_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC PRIMER_RIGHT_3_SEQUENCE=TTTCACTTCTCTTCACACCACTG PRIMER_LEFT_3=93,26 PRIMER_RIGHT_3=586,23 PRIMER_LEFT_3_TM=63.051 PRIMER_RIGHT_3_TM=59.429 PRIMER_LEFT_3_GC_PERCENT=38.462 PRIMER_RIGHT_3_GC_PERCENT=43.478 PRIMER_LEFT_3_SELF_ANY=8.00 PRIMER_RIGHT_3_SELF_ANY=3.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=3.00 PRIMER_LEFT_3_POSITION_PENALTY=0.000000 PRIMER_RIGHT_3_POSITION_PENALTY=2.000000 PRIMER_LEFT_3_END_STABILITY=6.4000 PRIMER_RIGHT_3_END_STABILITY=6.7000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_3=494 PRIMER_PAIR_PENALTY_4=15.8310 PRIMER_LEFT_4_PENALTY=9.051374 PRIMER_RIGHT_4_PENALTY=6.779595 PRIMER_LEFT_4_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC PRIMER_RIGHT_4_SEQUENCE=ATTTCACTTCTCTTCACACCACTG PRIMER_LEFT_4=93,26 PRIMER_RIGHT_4=587,24 PRIMER_LEFT_4_TM=63.051 PRIMER_RIGHT_4_TM=59.720 PRIMER_LEFT_4_GC_PERCENT=38.462 PRIMER_RIGHT_4_GC_PERCENT=41.667 PRIMER_LEFT_4_SELF_ANY=8.00 PRIMER_RIGHT_4_SELF_ANY=3.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=3.00 PRIMER_LEFT_4_POSITION_PENALTY=0.000000 PRIMER_RIGHT_4_POSITION_PENALTY=2.500000 PRIMER_LEFT_4_END_STABILITY=6.4000 PRIMER_RIGHT_4_END_STABILITY=6.7000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_4=495 PRIMER_PAIR_PENALTY_5=15.9173 PRIMER_LEFT_5_PENALTY=9.051374 PRIMER_RIGHT_5_PENALTY=6.865886 PRIMER_LEFT_5_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC PRIMER_RIGHT_5_SEQUENCE=CACTTCTCTTCACACCACTGGTTC PRIMER_LEFT_5=93,26 PRIMER_RIGHT_5=583,24 PRIMER_LEFT_5_TM=63.051 PRIMER_RIGHT_5_TM=62.366 PRIMER_LEFT_5_GC_PERCENT=38.462 PRIMER_RIGHT_5_GC_PERCENT=50.000 PRIMER_LEFT_5_SELF_ANY=8.00 PRIMER_RIGHT_5_SELF_ANY=7.00 PRIMER_LEFT_5_SELF_END=2.00 PRIMER_RIGHT_5_SELF_END=3.00 PRIMER_LEFT_5_POSITION_PENALTY=0.000000 PRIMER_RIGHT_5_POSITION_PENALTY=0.500000 PRIMER_LEFT_5_END_STABILITY=6.4000 PRIMER_RIGHT_5_END_STABILITY=7.9000 PRIMER_PAIR_5_COMPL_ANY=5.00 PRIMER_PAIR_5_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_5=491 PRIMER_PAIR_PENALTY_6=16.1955 PRIMER_LEFT_6_PENALTY=9.051374 PRIMER_RIGHT_6_PENALTY=7.144164 PRIMER_LEFT_6_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC PRIMER_RIGHT_6_SEQUENCE=CATTTCACTTCTCTTCACACCA PRIMER_LEFT_6=93,26 PRIMER_RIGHT_6=588,22 PRIMER_LEFT_6_TM=63.051 PRIMER_RIGHT_6_TM=57.856 PRIMER_LEFT_6_GC_PERCENT=38.462 PRIMER_RIGHT_6_GC_PERCENT=40.909 PRIMER_LEFT_6_SELF_ANY=8.00 PRIMER_RIGHT_6_SELF_ANY=2.00 PRIMER_LEFT_6_SELF_END=2.00 PRIMER_RIGHT_6_SELF_END=0.00 PRIMER_LEFT_6_POSITION_PENALTY=0.000000 PRIMER_RIGHT_6_POSITION_PENALTY=3.000000 PRIMER_LEFT_6_END_STABILITY=6.4000 PRIMER_RIGHT_6_END_STABILITY=8.2000 PRIMER_PAIR_6_COMPL_ANY=5.00 PRIMER_PAIR_6_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_6=496 PRIMER_PAIR_PENALTY_7=16.2938 PRIMER_LEFT_7_PENALTY=9.051374 PRIMER_RIGHT_7_PENALTY=7.242436 PRIMER_LEFT_7_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC PRIMER_RIGHT_7_SEQUENCE=CATTTCACTTCTCTTCACACCAC PRIMER_LEFT_7=93,26 PRIMER_RIGHT_7=588,23 PRIMER_LEFT_7_TM=63.051 PRIMER_RIGHT_7_TM=58.758 PRIMER_LEFT_7_GC_PERCENT=38.462 PRIMER_RIGHT_7_GC_PERCENT=43.478 PRIMER_LEFT_7_SELF_ANY=8.00 PRIMER_RIGHT_7_SELF_ANY=2.00 PRIMER_LEFT_7_SELF_END=2.00 PRIMER_RIGHT_7_SELF_END=0.00 PRIMER_LEFT_7_POSITION_PENALTY=0.000000 PRIMER_RIGHT_7_POSITION_PENALTY=3.000000 PRIMER_LEFT_7_END_STABILITY=6.4000 PRIMER_RIGHT_7_END_STABILITY=7.6000 PRIMER_PAIR_7_COMPL_ANY=5.00 PRIMER_PAIR_7_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_7=496 PRIMER_PAIR_PENALTY_8=16.3296 PRIMER_LEFT_8_PENALTY=9.051374 PRIMER_RIGHT_8_PENALTY=7.278253 PRIMER_LEFT_8_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC PRIMER_RIGHT_8_SEQUENCE=ACTTCTCTTCACACCACTGGTTCTC PRIMER_LEFT_8=93,26 PRIMER_RIGHT_8=582,25 PRIMER_LEFT_8_TM=63.051 PRIMER_RIGHT_8_TM=62.278 PRIMER_LEFT_8_GC_PERCENT=38.462 PRIMER_RIGHT_8_GC_PERCENT=48.000 PRIMER_LEFT_8_SELF_ANY=8.00 PRIMER_RIGHT_8_SELF_ANY=7.00 PRIMER_LEFT_8_SELF_END=2.00 PRIMER_RIGHT_8_SELF_END=1.00 PRIMER_LEFT_8_POSITION_PENALTY=0.000000 PRIMER_RIGHT_8_POSITION_PENALTY=0.000000 PRIMER_LEFT_8_END_STABILITY=6.4000 PRIMER_RIGHT_8_END_STABILITY=6.7000 PRIMER_PAIR_8_COMPL_ANY=5.00 PRIMER_PAIR_8_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_8=490 PRIMER_PAIR_PENALTY_9=16.3310 PRIMER_LEFT_9_PENALTY=9.051374 PRIMER_RIGHT_9_PENALTY=7.279595 PRIMER_LEFT_9_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC PRIMER_RIGHT_9_SEQUENCE=CATTTCACTTCTCTTCACACCACT PRIMER_LEFT_9=93,26 PRIMER_RIGHT_9=588,24 PRIMER_LEFT_9_TM=63.051 PRIMER_RIGHT_9_TM=59.720 PRIMER_LEFT_9_GC_PERCENT=38.462 PRIMER_RIGHT_9_GC_PERCENT=41.667 PRIMER_LEFT_9_SELF_ANY=8.00 PRIMER_RIGHT_9_SELF_ANY=2.00 PRIMER_LEFT_9_SELF_END=2.00 PRIMER_RIGHT_9_SELF_END=1.00 PRIMER_LEFT_9_POSITION_PENALTY=0.000000 PRIMER_RIGHT_9_POSITION_PENALTY=3.000000 PRIMER_LEFT_9_END_STABILITY=6.4000 PRIMER_RIGHT_9_END_STABILITY=7.9000 PRIMER_PAIR_9_COMPL_ANY=5.00 PRIMER_PAIR_9_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_9=496 PRIMER_PAIR_PENALTY_10=16.7938 PRIMER_LEFT_10_PENALTY=9.051374 PRIMER_RIGHT_10_PENALTY=7.742436 PRIMER_LEFT_10_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC PRIMER_RIGHT_10_SEQUENCE=ACATTTCACTTCTCTTCACACCA PRIMER_LEFT_10=93,26 PRIMER_RIGHT_10=589,23 PRIMER_LEFT_10_TM=63.051 PRIMER_RIGHT_10_TM=58.758 PRIMER_LEFT_10_GC_PERCENT=38.462 PRIMER_RIGHT_10_GC_PERCENT=39.130 PRIMER_LEFT_10_SELF_ANY=8.00 PRIMER_RIGHT_10_SELF_ANY=2.00 PRIMER_LEFT_10_SELF_END=2.00 PRIMER_RIGHT_10_SELF_END=0.00 PRIMER_LEFT_10_POSITION_PENALTY=0.000000 PRIMER_RIGHT_10_POSITION_PENALTY=3.500000 PRIMER_LEFT_10_END_STABILITY=6.4000 PRIMER_RIGHT_10_END_STABILITY=8.2000 PRIMER_PAIR_10_COMPL_ANY=5.00 PRIMER_PAIR_10_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_10=497 PRIMER_PAIR_PENALTY_11=16.9598 PRIMER_LEFT_11_PENALTY=9.051374 PRIMER_RIGHT_11_PENALTY=7.908407 PRIMER_LEFT_11_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC PRIMER_RIGHT_11_SEQUENCE=ACATTTCACTTCTCTTCACACCAC PRIMER_LEFT_11=93,26 PRIMER_RIGHT_11=589,24 PRIMER_LEFT_11_TM=63.051 PRIMER_RIGHT_11_TM=59.592 PRIMER_LEFT_11_GC_PERCENT=38.462 PRIMER_RIGHT_11_GC_PERCENT=41.667 PRIMER_LEFT_11_SELF_ANY=8.00 PRIMER_RIGHT_11_SELF_ANY=2.00 PRIMER_LEFT_11_SELF_END=2.00 PRIMER_RIGHT_11_SELF_END=0.00 PRIMER_LEFT_11_POSITION_PENALTY=0.000000 PRIMER_RIGHT_11_POSITION_PENALTY=3.500000 PRIMER_LEFT_11_END_STABILITY=6.4000 PRIMER_RIGHT_11_END_STABILITY=7.6000 PRIMER_PAIR_11_COMPL_ANY=5.00 PRIMER_PAIR_11_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_11=497 PRIMER_PAIR_PENALTY_12=17.1262 PRIMER_LEFT_12_PENALTY=9.051374 PRIMER_RIGHT_12_PENALTY=8.074807 PRIMER_LEFT_12_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC PRIMER_RIGHT_12_SEQUENCE=AACATTTCACTTCTCTTCACACCA PRIMER_LEFT_12=93,26 PRIMER_RIGHT_12=590,24 PRIMER_LEFT_12_TM=63.051 PRIMER_RIGHT_12_TM=60.075 PRIMER_LEFT_12_GC_PERCENT=38.462 PRIMER_RIGHT_12_GC_PERCENT=37.500 PRIMER_LEFT_12_SELF_ANY=8.00 PRIMER_RIGHT_12_SELF_ANY=2.00 PRIMER_LEFT_12_SELF_END=2.00 PRIMER_RIGHT_12_SELF_END=0.00 PRIMER_LEFT_12_POSITION_PENALTY=0.000000 PRIMER_RIGHT_12_POSITION_PENALTY=4.000000 PRIMER_LEFT_12_END_STABILITY=6.4000 PRIMER_RIGHT_12_END_STABILITY=8.2000 PRIMER_PAIR_12_COMPL_ANY=5.00 PRIMER_PAIR_12_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_12=498 PRIMER_PAIR_PENALTY_13=17.5496 PRIMER_LEFT_13_PENALTY=9.051374 PRIMER_RIGHT_13_PENALTY=8.498271 PRIMER_LEFT_13_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC PRIMER_RIGHT_13_SEQUENCE=ATTTCACTTCTCTTCACACCACT PRIMER_LEFT_13=93,26 PRIMER_RIGHT_13=587,23 PRIMER_LEFT_13_TM=63.051 PRIMER_RIGHT_13_TM=57.002 PRIMER_LEFT_13_GC_PERCENT=38.462 PRIMER_RIGHT_13_GC_PERCENT=39.130 PRIMER_LEFT_13_SELF_ANY=8.00 PRIMER_RIGHT_13_SELF_ANY=2.00 PRIMER_LEFT_13_SELF_END=2.00 PRIMER_RIGHT_13_SELF_END=1.00 PRIMER_LEFT_13_POSITION_PENALTY=0.000000 PRIMER_RIGHT_13_POSITION_PENALTY=2.500000 PRIMER_LEFT_13_END_STABILITY=6.4000 PRIMER_RIGHT_13_END_STABILITY=7.9000 PRIMER_PAIR_13_COMPL_ANY=5.00 PRIMER_PAIR_13_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_13=495 PRIMER_PAIR_PENALTY_14=17.6262 PRIMER_LEFT_14_PENALTY=9.051374 PRIMER_RIGHT_14_PENALTY=8.574807 PRIMER_LEFT_14_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC PRIMER_RIGHT_14_SEQUENCE=CAACATTTCACTTCTCTTCACACC PRIMER_LEFT_14=93,26 PRIMER_RIGHT_14=591,24 PRIMER_LEFT_14_TM=63.051 PRIMER_RIGHT_14_TM=60.075 PRIMER_LEFT_14_GC_PERCENT=38.462 PRIMER_RIGHT_14_GC_PERCENT=41.667 PRIMER_LEFT_14_SELF_ANY=8.00 PRIMER_RIGHT_14_SELF_ANY=2.00 PRIMER_LEFT_14_SELF_END=2.00 PRIMER_RIGHT_14_SELF_END=0.00 PRIMER_LEFT_14_POSITION_PENALTY=0.000000 PRIMER_RIGHT_14_POSITION_PENALTY=4.500000 PRIMER_LEFT_14_END_STABILITY=6.4000 PRIMER_RIGHT_14_END_STABILITY=7.6000 PRIMER_PAIR_14_COMPL_ANY=5.00 PRIMER_PAIR_14_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_14=499 PRIMER_PAIR_PENALTY_15=17.6905 PRIMER_LEFT_15_PENALTY=9.051374 PRIMER_RIGHT_15_PENALTY=8.639124 PRIMER_LEFT_15_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC PRIMER_RIGHT_15_SEQUENCE=CACTTCTCTTCACACCACTGGTTCT PRIMER_LEFT_15=93,26 PRIMER_RIGHT_15=583,25 PRIMER_LEFT_15_TM=63.051 PRIMER_RIGHT_15_TM=63.139 PRIMER_LEFT_15_GC_PERCENT=38.462 PRIMER_RIGHT_15_GC_PERCENT=48.000 PRIMER_LEFT_15_SELF_ANY=8.00 PRIMER_RIGHT_15_SELF_ANY=7.00 PRIMER_LEFT_15_SELF_END=2.00 PRIMER_RIGHT_15_SELF_END=3.00 PRIMER_LEFT_15_POSITION_PENALTY=0.000000 PRIMER_RIGHT_15_POSITION_PENALTY=0.500000 PRIMER_LEFT_15_END_STABILITY=6.4000 PRIMER_RIGHT_15_END_STABILITY=6.4000 PRIMER_PAIR_15_COMPL_ANY=5.00 PRIMER_PAIR_15_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_15=491 PRIMER_PAIR_PENALTY_16=18.0352 PRIMER_LEFT_16_PENALTY=9.051374 PRIMER_RIGHT_16_PENALTY=8.983834 PRIMER_LEFT_16_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC PRIMER_RIGHT_16_SEQUENCE=ACATTTCACTTCTCTTCACACCACT PRIMER_LEFT_16=93,26 PRIMER_RIGHT_16=589,25 PRIMER_LEFT_16_TM=63.051 PRIMER_RIGHT_16_TM=60.484 PRIMER_LEFT_16_GC_PERCENT=38.462 PRIMER_RIGHT_16_GC_PERCENT=40.000 PRIMER_LEFT_16_SELF_ANY=8.00 PRIMER_RIGHT_16_SELF_ANY=2.00 PRIMER_LEFT_16_SELF_END=2.00 PRIMER_RIGHT_16_SELF_END=1.00 PRIMER_LEFT_16_POSITION_PENALTY=0.000000 PRIMER_RIGHT_16_POSITION_PENALTY=3.500000 PRIMER_LEFT_16_END_STABILITY=6.4000 PRIMER_RIGHT_16_END_STABILITY=7.9000 PRIMER_PAIR_16_COMPL_ANY=5.00 PRIMER_PAIR_16_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_16=497 PRIMER_PAIR_PENALTY_17=18.0736 PRIMER_LEFT_17_PENALTY=9.051374 PRIMER_RIGHT_17_PENALTY=9.022192 PRIMER_LEFT_17_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC PRIMER_RIGHT_17_SEQUENCE=ACTTCTCTTCACACCACTGGTTCTCT PRIMER_LEFT_17=93,26 PRIMER_RIGHT_17=582,26 PRIMER_LEFT_17_TM=63.051 PRIMER_RIGHT_17_TM=63.022 PRIMER_LEFT_17_GC_PERCENT=38.462 PRIMER_RIGHT_17_GC_PERCENT=46.154 PRIMER_LEFT_17_SELF_ANY=8.00 PRIMER_RIGHT_17_SELF_ANY=7.00 PRIMER_LEFT_17_SELF_END=2.00 PRIMER_RIGHT_17_SELF_END=0.00 PRIMER_LEFT_17_POSITION_PENALTY=0.000000 PRIMER_RIGHT_17_POSITION_PENALTY=0.000000 PRIMER_LEFT_17_END_STABILITY=6.4000 PRIMER_RIGHT_17_END_STABILITY=6.4000 PRIMER_PAIR_17_COMPL_ANY=5.00 PRIMER_PAIR_17_COMPL_END=3.00 PRIMER_PRODUCT_SIZE_17=490 PRIMER_PAIR_PENALTY_18=18.6362 PRIMER_LEFT_18_PENALTY=9.051374 PRIMER_RIGHT_18_PENALTY=9.584808 PRIMER_LEFT_18_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC PRIMER_RIGHT_18_SEQUENCE=GCACAACATTTCACTTCTCTTCA PRIMER_LEFT_18=93,26 PRIMER_RIGHT_18=594,23 PRIMER_LEFT_18_TM=63.051 PRIMER_RIGHT_18_TM=59.415 PRIMER_LEFT_18_GC_PERCENT=38.462 PRIMER_RIGHT_18_GC_PERCENT=39.130 PRIMER_LEFT_18_SELF_ANY=8.00 PRIMER_RIGHT_18_SELF_ANY=2.00 PRIMER_LEFT_18_SELF_END=2.00 PRIMER_RIGHT_18_SELF_END=1.00 PRIMER_LEFT_18_POSITION_PENALTY=0.000000 PRIMER_RIGHT_18_POSITION_PENALTY=6.000000 PRIMER_LEFT_18_END_STABILITY=6.4000 PRIMER_RIGHT_18_END_STABILITY=7.0000 PRIMER_PAIR_18_COMPL_ANY=5.00 PRIMER_PAIR_18_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_18=502 PRIMER_PAIR_PENALTY_19=18.6661 PRIMER_LEFT_19_PENALTY=9.051374 PRIMER_RIGHT_19_PENALTY=9.614678 PRIMER_LEFT_19_SEQUENCE=ATGCCTTCAATTAAGTTGCAGAGTTC PRIMER_RIGHT_19_SEQUENCE=AACATTTCACTTCTCTTCACACC PRIMER_LEFT_19=93,26 PRIMER_RIGHT_19=590,23 PRIMER_LEFT_19_TM=63.051 PRIMER_RIGHT_19_TM=57.385 PRIMER_LEFT_19_GC_PERCENT=38.462 PRIMER_RIGHT_19_GC_PERCENT=39.130 PRIMER_LEFT_19_SELF_ANY=8.00 PRIMER_RIGHT_19_SELF_ANY=2.00 PRIMER_LEFT_19_SELF_END=2.00 PRIMER_RIGHT_19_SELF_END=0.00 PRIMER_LEFT_19_POSITION_PENALTY=0.000000 PRIMER_RIGHT_19_POSITION_PENALTY=4.000000 PRIMER_LEFT_19_END_STABILITY=6.4000 PRIMER_RIGHT_19_END_STABILITY=7.6000 PRIMER_PAIR_19_COMPL_ANY=5.00 PRIMER_PAIR_19_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_19=498 = PRIMER_SEQUENCE_ID=X80343/P35 PRIMER_COMMENT=H.sapiens p35 mRNA for regulatory subunit of cdk5 kinase. CDS=98..1021 PRIMER_START_CODON_POSITION=97 SEQUENCE=AAACTCAGAATTTTCGCGGGCTCGGTGAGCGGTTTTATCCCTCCGGCCGGCAGGCTGGGCGCAGGGGGCGAGCCCCCGCCCGGCGCGCAGCAGCACCATGGGCACGGTGCTGTCCCTGTCTCCCAGCTACCGGAAGGCCACGCTGTTTGAGGATGGCGCGGCCACCGTGGGCCACTATACGGCCGTACAGAACAGCAAGAACGCCAAGGACAAGAACCTGAAGCGCCACTCCATCATCTCCGTGCTGCCTTGGAAGAGAATCGTGGCCGTGTCGGCCAAGAAGAAGAACTCCAAGAAGGTGCAGCCTAACAGCAGCTACCAGAACAACATCACGCACCTCAACAATGAGAACCTGAAGAAGTCGCTGTCGTGCGCCAACCTGTCCACATTCGCCCAGCCCCCACCGGCCCAGCCGCCTGCACCCCCGGCCAGCCAGCTCTCGGGTTCCCAGACCGGGGGCTCCTCCTCAGTCAAGAAAGCCCCTCACCCTGCCGTCACCTCCGCAGGGACGCCCAAACGGGTCATCGTCCAGGCGTCCACCAGTGAGCTGCTTCGCTGCCTGGGTGAGTTTCTCTGCCGCCGGTGCTACCGCCTGAAGCACCTGTCCCCCACGGACCCCGTGCTCTGGCTGCGCAGCGTGGACCGCTCGCTGCTTCTGCAGGGCTGGCAGGACCAGGGCTTCATCACGCCGGCCAACGTGGTCTTCCTCTACATGCTCTGCAGGGATGTTATCTCCTCCGAGGTGGGCTCGGATCACGAGCTCCAGGCCGTCCTGCTGACATGCCTGTACCTCTCCTACTCCTACATGGGCAACGAGATCTCCTACCCGCTCAAGCCCTTCCTGGTGGAGAGCTGCAAGGAGGCCTTTTGGGACCGTTGCCTCTCTGTCATCAACCTCATGAGCTCAAAGATGCTGCAGATAAATGCCGACCCACACTACTTCACACAGGTCTTCTCCGACCTGAAGAACGAGAGCGGCCAGGAGGACAAGAAGCGGCTCCTCCTAGGCCTGGATCGGTGAGCACTGTAGCCTGCGTCATGGCTCAAGGATTCAATGCATTTTTAAGAATTTATTATTAAATCAGTTTTGTGTACAG PRIMER_LEFT_EXPLAIN=considered 8968, would not amplify any of the ORF 6063, GC content failed 212, low tm 125, high tm 1996, high 3' stability 33, ok 539 PRIMER_RIGHT_EXPLAIN=considered 8912, GC content failed 682, low tm 430, high tm 6084, high end compl 14, high 3' stability 37, ok 1665 PRIMER_PAIR_EXPLAIN=considered 26, high end compl 4, ok 22 PRIMER_STOP_CODON_POSITION=1018 PRIMER_PAIR_PENALTY=127.4669 PRIMER_LEFT_PENALTY=122.322207 PRIMER_RIGHT_PENALTY=5.144675 PRIMER_LEFT_SEQUENCE=ACGGTGCTGTCCCTGTCTC PRIMER_RIGHT_SEQUENCE=TACAGTGCTCACCGATCCAG PRIMER_LEFT=103,19 PRIMER_RIGHT=1028,20 PRIMER_LEFT_TM=61.322 PRIMER_RIGHT_TM=59.855 PRIMER_LEFT_GC_PERCENT=63.158 PRIMER_RIGHT_GC_PERCENT=55.000 PRIMER_LEFT_SELF_ANY=3.00 PRIMER_RIGHT_SELF_ANY=4.00 PRIMER_LEFT_SELF_END=0.00 PRIMER_RIGHT_SELF_END=1.00 PRIMER_LEFT_POSITION_PENALTY=120.000000 PRIMER_RIGHT_POSITION_PENALTY=5.000000 PRIMER_LEFT_END_STABILITY=6.1000 PRIMER_RIGHT_END_STABILITY=8.2000 PRIMER_PAIR_COMPL_ANY=5.00 PRIMER_PAIR_COMPL_END=3.00 PRIMER_PRODUCT_SIZE=926 PRIMER_PAIR_PENALTY_1=127.9669 PRIMER_LEFT_1_PENALTY=122.322207 PRIMER_RIGHT_1_PENALTY=5.644675 PRIMER_LEFT_1_SEQUENCE=ACGGTGCTGTCCCTGTCTC PRIMER_RIGHT_1_SEQUENCE=CTACAGTGCTCACCGATCCA PRIMER_LEFT_1=103,19 PRIMER_RIGHT_1=1029,20 PRIMER_LEFT_1_TM=61.322 PRIMER_RIGHT_1_TM=59.855 PRIMER_LEFT_1_GC_PERCENT=63.158 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=3.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=0.00 PRIMER_LEFT_1_POSITION_PENALTY=120.000000 PRIMER_RIGHT_1_POSITION_PENALTY=5.500000 PRIMER_LEFT_1_END_STABILITY=6.1000 PRIMER_RIGHT_1_END_STABILITY=8.1000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_1=927 PRIMER_PAIR_PENALTY_2=127.9856 PRIMER_LEFT_2_PENALTY=122.322207 PRIMER_RIGHT_2_PENALTY=5.663428 PRIMER_LEFT_2_SEQUENCE=ACGGTGCTGTCCCTGTCTC PRIMER_RIGHT_2_SEQUENCE=ACAGTGCTCACCGATCCAG PRIMER_LEFT_2=103,19 PRIMER_RIGHT_2=1027,19 PRIMER_LEFT_2_TM=61.322 PRIMER_RIGHT_2_TM=59.837 PRIMER_LEFT_2_GC_PERCENT=63.158 PRIMER_RIGHT_2_GC_PERCENT=57.895 PRIMER_LEFT_2_SELF_ANY=3.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=1.00 PRIMER_LEFT_2_POSITION_PENALTY=120.000000 PRIMER_RIGHT_2_POSITION_PENALTY=4.500000 PRIMER_LEFT_2_END_STABILITY=6.1000 PRIMER_RIGHT_2_END_STABILITY=8.2000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=3.00 PRIMER_PRODUCT_SIZE_2=925 PRIMER_PAIR_PENALTY_3=129.1555 PRIMER_LEFT_3_PENALTY=122.322207 PRIMER_RIGHT_3_PENALTY=6.833259 PRIMER_LEFT_3_SEQUENCE=ACGGTGCTGTCCCTGTCTC PRIMER_RIGHT_3_SEQUENCE=GCTACAGTGCTCACCGATCC PRIMER_LEFT_3=103,19 PRIMER_RIGHT_3=1030,20 PRIMER_LEFT_3_TM=61.322 PRIMER_RIGHT_3_TM=60.833 PRIMER_LEFT_3_GC_PERCENT=63.158 PRIMER_RIGHT_3_GC_PERCENT=60.000 PRIMER_LEFT_3_SELF_ANY=3.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_LEFT_3_POSITION_PENALTY=120.000000 PRIMER_RIGHT_3_POSITION_PENALTY=6.000000 PRIMER_LEFT_3_END_STABILITY=6.1000 PRIMER_RIGHT_3_END_STABILITY=7.8000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=3.00 PRIMER_PRODUCT_SIZE_3=928 PRIMER_PAIR_PENALTY_4=129.4246 PRIMER_LEFT_4_PENALTY=122.322207 PRIMER_RIGHT_4_PENALTY=7.102380 PRIMER_LEFT_4_SEQUENCE=ACGGTGCTGTCCCTGTCTC PRIMER_RIGHT_4_SEQUENCE=AGGCTACAGTGCTCACCGAT PRIMER_LEFT_4=103,19 PRIMER_RIGHT_4=1032,20 PRIMER_LEFT_4_TM=61.322 PRIMER_RIGHT_4_TM=59.898 PRIMER_LEFT_4_GC_PERCENT=63.158 PRIMER_RIGHT_4_GC_PERCENT=55.000 PRIMER_LEFT_4_SELF_ANY=3.00 PRIMER_RIGHT_4_SELF_ANY=4.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_LEFT_4_POSITION_PENALTY=120.000000 PRIMER_RIGHT_4_POSITION_PENALTY=7.000000 PRIMER_LEFT_4_END_STABILITY=6.1000 PRIMER_RIGHT_4_END_STABILITY=9.8000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=3.00 PRIMER_PRODUCT_SIZE_4=930 PRIMER_PAIR_PENALTY_5=129.5803 PRIMER_LEFT_5_PENALTY=122.322207 PRIMER_RIGHT_5_PENALTY=7.258073 PRIMER_LEFT_5_SEQUENCE=ACGGTGCTGTCCCTGTCTC PRIMER_RIGHT_5_SEQUENCE=TACAGTGCTCACCGATCCA PRIMER_LEFT_5=103,19 PRIMER_RIGHT_5=1028,19 PRIMER_LEFT_5_TM=61.322 PRIMER_RIGHT_5_TM=58.742 PRIMER_LEFT_5_GC_PERCENT=63.158 PRIMER_RIGHT_5_GC_PERCENT=52.632 PRIMER_LEFT_5_SELF_ANY=3.00 PRIMER_RIGHT_5_SELF_ANY=4.00 PRIMER_LEFT_5_SELF_END=0.00 PRIMER_RIGHT_5_SELF_END=0.00 PRIMER_LEFT_5_POSITION_PENALTY=120.000000 PRIMER_RIGHT_5_POSITION_PENALTY=5.000000 PRIMER_LEFT_5_END_STABILITY=6.1000 PRIMER_RIGHT_5_END_STABILITY=8.1000 PRIMER_PAIR_5_COMPL_ANY=5.00 PRIMER_PAIR_5_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_5=926 PRIMER_PAIR_PENALTY_6=129.6813 PRIMER_LEFT_6_PENALTY=122.322207 PRIMER_RIGHT_6_PENALTY=7.359137 PRIMER_LEFT_6_SEQUENCE=ACGGTGCTGTCCCTGTCTC PRIMER_RIGHT_6_SEQUENCE=CTACAGTGCTCACCGATCCAG PRIMER_LEFT_6=103,19 PRIMER_RIGHT_6=1029,21 PRIMER_LEFT_6_TM=61.322 PRIMER_RIGHT_6_TM=60.859 PRIMER_LEFT_6_GC_PERCENT=63.158 PRIMER_RIGHT_6_GC_PERCENT=57.143 PRIMER_LEFT_6_SELF_ANY=3.00 PRIMER_RIGHT_6_SELF_ANY=4.00 PRIMER_LEFT_6_SELF_END=0.00 PRIMER_RIGHT_6_SELF_END=1.00 PRIMER_LEFT_6_POSITION_PENALTY=120.000000 PRIMER_RIGHT_6_POSITION_PENALTY=5.500000 PRIMER_LEFT_6_END_STABILITY=6.1000 PRIMER_RIGHT_6_END_STABILITY=8.2000 PRIMER_PAIR_6_COMPL_ANY=5.00 PRIMER_PAIR_6_COMPL_END=3.00 PRIMER_PRODUCT_SIZE_6=927 PRIMER_PAIR_PENALTY_7=130.1180 PRIMER_LEFT_7_PENALTY=124.973289 PRIMER_RIGHT_7_PENALTY=5.144675 PRIMER_LEFT_7_SEQUENCE=ACGGTGCTGTCCCTGTCTCC PRIMER_RIGHT_7_SEQUENCE=TACAGTGCTCACCGATCCAG PRIMER_LEFT_7=103,20 PRIMER_RIGHT_7=1028,20 PRIMER_LEFT_7_TM=64.973 PRIMER_RIGHT_7_TM=59.855 PRIMER_LEFT_7_GC_PERCENT=65.000 PRIMER_RIGHT_7_GC_PERCENT=55.000 PRIMER_LEFT_7_SELF_ANY=3.00 PRIMER_RIGHT_7_SELF_ANY=4.00 PRIMER_LEFT_7_SELF_END=0.00 PRIMER_RIGHT_7_SELF_END=1.00 PRIMER_LEFT_7_POSITION_PENALTY=120.000000 PRIMER_RIGHT_7_POSITION_PENALTY=5.000000 PRIMER_LEFT_7_END_STABILITY=7.9000 PRIMER_RIGHT_7_END_STABILITY=8.2000 PRIMER_PAIR_7_COMPL_ANY=5.00 PRIMER_PAIR_7_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_7=926 PRIMER_PAIR_PENALTY_8=130.6021 PRIMER_LEFT_8_PENALTY=122.322207 PRIMER_RIGHT_8_PENALTY=8.279921 PRIMER_LEFT_8_SEQUENCE=ACGGTGCTGTCCCTGTCTC PRIMER_RIGHT_8_SEQUENCE=AGTGCTCACCGATCCAGGC PRIMER_LEFT_8=103,19 PRIMER_RIGHT_8=1025,19 PRIMER_LEFT_8_TM=61.322 PRIMER_RIGHT_8_TM=63.780 PRIMER_LEFT_8_GC_PERCENT=63.158 PRIMER_RIGHT_8_GC_PERCENT=63.158 PRIMER_LEFT_8_SELF_ANY=3.00 PRIMER_RIGHT_8_SELF_ANY=4.00 PRIMER_LEFT_8_SELF_END=0.00 PRIMER_RIGHT_8_SELF_END=2.00 PRIMER_LEFT_8_POSITION_PENALTY=120.000000 PRIMER_RIGHT_8_POSITION_PENALTY=3.500000 PRIMER_LEFT_8_END_STABILITY=6.1000 PRIMER_RIGHT_8_END_STABILITY=9.7000 PRIMER_PAIR_8_COMPL_ANY=5.00 PRIMER_PAIR_8_COMPL_END=3.00 PRIMER_PRODUCT_SIZE_8=923 PRIMER_PAIR_PENALTY_9=130.6180 PRIMER_LEFT_9_PENALTY=124.973289 PRIMER_RIGHT_9_PENALTY=5.644675 PRIMER_LEFT_9_SEQUENCE=ACGGTGCTGTCCCTGTCTCC PRIMER_RIGHT_9_SEQUENCE=CTACAGTGCTCACCGATCCA PRIMER_LEFT_9=103,20 PRIMER_RIGHT_9=1029,20 PRIMER_LEFT_9_TM=64.973 PRIMER_RIGHT_9_TM=59.855 PRIMER_LEFT_9_GC_PERCENT=65.000 PRIMER_RIGHT_9_GC_PERCENT=55.000 PRIMER_LEFT_9_SELF_ANY=3.00 PRIMER_RIGHT_9_SELF_ANY=4.00 PRIMER_LEFT_9_SELF_END=0.00 PRIMER_RIGHT_9_SELF_END=0.00 PRIMER_LEFT_9_POSITION_PENALTY=120.000000 PRIMER_RIGHT_9_POSITION_PENALTY=5.500000 PRIMER_LEFT_9_END_STABILITY=7.9000 PRIMER_RIGHT_9_END_STABILITY=8.1000 PRIMER_PAIR_9_COMPL_ANY=5.00 PRIMER_PAIR_9_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_9=927 PRIMER_PAIR_PENALTY_10=130.6367 PRIMER_LEFT_10_PENALTY=124.973289 PRIMER_RIGHT_10_PENALTY=5.663428 PRIMER_LEFT_10_SEQUENCE=ACGGTGCTGTCCCTGTCTCC PRIMER_RIGHT_10_SEQUENCE=ACAGTGCTCACCGATCCAG PRIMER_LEFT_10=103,20 PRIMER_RIGHT_10=1027,19 PRIMER_LEFT_10_TM=64.973 PRIMER_RIGHT_10_TM=59.837 PRIMER_LEFT_10_GC_PERCENT=65.000 PRIMER_RIGHT_10_GC_PERCENT=57.895 PRIMER_LEFT_10_SELF_ANY=3.00 PRIMER_RIGHT_10_SELF_ANY=4.00 PRIMER_LEFT_10_SELF_END=0.00 PRIMER_RIGHT_10_SELF_END=1.00 PRIMER_LEFT_10_POSITION_PENALTY=120.000000 PRIMER_RIGHT_10_POSITION_PENALTY=4.500000 PRIMER_LEFT_10_END_STABILITY=7.9000 PRIMER_RIGHT_10_END_STABILITY=8.2000 PRIMER_PAIR_10_COMPL_ANY=5.00 PRIMER_PAIR_10_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_10=925 PRIMER_PAIR_PENALTY_11=130.7353 PRIMER_LEFT_11_PENALTY=122.322207 PRIMER_RIGHT_11_PENALTY=8.413090 PRIMER_LEFT_11_SEQUENCE=ACGGTGCTGTCCCTGTCTC PRIMER_RIGHT_11_SEQUENCE=AGGCTACAGTGCTCACCGA PRIMER_LEFT_11=103,19 PRIMER_RIGHT_11=1032,19 PRIMER_LEFT_11_TM=61.322 PRIMER_RIGHT_11_TM=59.587 PRIMER_LEFT_11_GC_PERCENT=63.158 PRIMER_RIGHT_11_GC_PERCENT=57.895 PRIMER_LEFT_11_SELF_ANY=3.00 PRIMER_RIGHT_11_SELF_ANY=4.00 PRIMER_LEFT_11_SELF_END=0.00 PRIMER_RIGHT_11_SELF_END=2.00 PRIMER_LEFT_11_POSITION_PENALTY=120.000000 PRIMER_RIGHT_11_POSITION_PENALTY=7.000000 PRIMER_LEFT_11_END_STABILITY=6.1000 PRIMER_RIGHT_11_END_STABILITY=9.6000 PRIMER_PAIR_11_COMPL_ANY=5.00 PRIMER_PAIR_11_COMPL_END=3.00 PRIMER_PRODUCT_SIZE_11=930 PRIMER_PAIR_PENALTY_12=130.9774 PRIMER_LEFT_12_PENALTY=122.322207 PRIMER_RIGHT_12_PENALTY=8.655174 PRIMER_LEFT_12_SEQUENCE=ACGGTGCTGTCCCTGTCTC PRIMER_RIGHT_12_SEQUENCE=GGCTACAGTGCTCACCGAT PRIMER_LEFT_12=103,19 PRIMER_RIGHT_12=1031,19 PRIMER_LEFT_12_TM=61.322 PRIMER_RIGHT_12_TM=58.845 PRIMER_LEFT_12_GC_PERCENT=63.158 PRIMER_RIGHT_12_GC_PERCENT=57.895 PRIMER_LEFT_12_SELF_ANY=3.00 PRIMER_RIGHT_12_SELF_ANY=4.00 PRIMER_LEFT_12_SELF_END=0.00 PRIMER_RIGHT_12_SELF_END=2.00 PRIMER_LEFT_12_POSITION_PENALTY=120.000000 PRIMER_RIGHT_12_POSITION_PENALTY=6.500000 PRIMER_LEFT_12_END_STABILITY=6.1000 PRIMER_RIGHT_12_END_STABILITY=9.8000 PRIMER_PAIR_12_COMPL_ANY=5.00 PRIMER_PAIR_12_COMPL_END=3.00 PRIMER_PRODUCT_SIZE_12=929 PRIMER_PAIR_PENALTY_13=131.7293 PRIMER_LEFT_13_PENALTY=122.322207 PRIMER_RIGHT_13_PENALTY=9.407131 PRIMER_LEFT_13_SEQUENCE=ACGGTGCTGTCCCTGTCTC PRIMER_RIGHT_13_SEQUENCE=GCAGGCTACAGTGCTCACC PRIMER_LEFT_13=103,19 PRIMER_RIGHT_13=1034,19 PRIMER_LEFT_13_TM=61.322 PRIMER_RIGHT_13_TM=59.593 PRIMER_LEFT_13_GC_PERCENT=63.158 PRIMER_RIGHT_13_GC_PERCENT=63.158 PRIMER_LEFT_13_SELF_ANY=3.00 PRIMER_RIGHT_13_SELF_ANY=4.00 PRIMER_LEFT_13_SELF_END=0.00 PRIMER_RIGHT_13_SELF_END=2.00 PRIMER_LEFT_13_POSITION_PENALTY=120.000000 PRIMER_RIGHT_13_POSITION_PENALTY=8.000000 PRIMER_LEFT_13_END_STABILITY=6.1000 PRIMER_RIGHT_13_END_STABILITY=7.9000 PRIMER_PAIR_13_COMPL_ANY=5.00 PRIMER_PAIR_13_COMPL_END=3.00 PRIMER_PRODUCT_SIZE_13=932 PRIMER_PAIR_PENALTY_14=131.8065 PRIMER_LEFT_14_PENALTY=124.973289 PRIMER_RIGHT_14_PENALTY=6.833259 PRIMER_LEFT_14_SEQUENCE=ACGGTGCTGTCCCTGTCTCC PRIMER_RIGHT_14_SEQUENCE=GCTACAGTGCTCACCGATCC PRIMER_LEFT_14=103,20 PRIMER_RIGHT_14=1030,20 PRIMER_LEFT_14_TM=64.973 PRIMER_RIGHT_14_TM=60.833 PRIMER_LEFT_14_GC_PERCENT=65.000 PRIMER_RIGHT_14_GC_PERCENT=60.000 PRIMER_LEFT_14_SELF_ANY=3.00 PRIMER_RIGHT_14_SELF_ANY=4.00 PRIMER_LEFT_14_SELF_END=0.00 PRIMER_RIGHT_14_SELF_END=2.00 PRIMER_LEFT_14_POSITION_PENALTY=120.000000 PRIMER_RIGHT_14_POSITION_PENALTY=6.000000 PRIMER_LEFT_14_END_STABILITY=7.9000 PRIMER_RIGHT_14_END_STABILITY=7.8000 PRIMER_PAIR_14_COMPL_ANY=5.00 PRIMER_PAIR_14_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_14=928 PRIMER_PAIR_PENALTY_15=132.2314 PRIMER_LEFT_15_PENALTY=124.973289 PRIMER_RIGHT_15_PENALTY=7.258073 PRIMER_LEFT_15_SEQUENCE=ACGGTGCTGTCCCTGTCTCC PRIMER_RIGHT_15_SEQUENCE=TACAGTGCTCACCGATCCA PRIMER_LEFT_15=103,20 PRIMER_RIGHT_15=1028,19 PRIMER_LEFT_15_TM=64.973 PRIMER_RIGHT_15_TM=58.742 PRIMER_LEFT_15_GC_PERCENT=65.000 PRIMER_RIGHT_15_GC_PERCENT=52.632 PRIMER_LEFT_15_SELF_ANY=3.00 PRIMER_RIGHT_15_SELF_ANY=4.00 PRIMER_LEFT_15_SELF_END=0.00 PRIMER_RIGHT_15_SELF_END=0.00 PRIMER_LEFT_15_POSITION_PENALTY=120.000000 PRIMER_RIGHT_15_POSITION_PENALTY=5.000000 PRIMER_LEFT_15_END_STABILITY=7.9000 PRIMER_RIGHT_15_END_STABILITY=8.1000 PRIMER_PAIR_15_COMPL_ANY=5.00 PRIMER_PAIR_15_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_15=926 PRIMER_PAIR_PENALTY_16=132.3324 PRIMER_LEFT_16_PENALTY=124.973289 PRIMER_RIGHT_16_PENALTY=7.359137 PRIMER_LEFT_16_SEQUENCE=ACGGTGCTGTCCCTGTCTCC PRIMER_RIGHT_16_SEQUENCE=CTACAGTGCTCACCGATCCAG PRIMER_LEFT_16=103,20 PRIMER_RIGHT_16=1029,21 PRIMER_LEFT_16_TM=64.973 PRIMER_RIGHT_16_TM=60.859 PRIMER_LEFT_16_GC_PERCENT=65.000 PRIMER_RIGHT_16_GC_PERCENT=57.143 PRIMER_LEFT_16_SELF_ANY=3.00 PRIMER_RIGHT_16_SELF_ANY=4.00 PRIMER_LEFT_16_SELF_END=0.00 PRIMER_RIGHT_16_SELF_END=1.00 PRIMER_LEFT_16_POSITION_PENALTY=120.000000 PRIMER_RIGHT_16_POSITION_PENALTY=5.500000 PRIMER_LEFT_16_END_STABILITY=7.9000 PRIMER_RIGHT_16_END_STABILITY=8.2000 PRIMER_PAIR_16_COMPL_ANY=5.00 PRIMER_PAIR_16_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_16=927 PRIMER_PAIR_PENALTY_17=132.3926 PRIMER_LEFT_17_PENALTY=122.322207 PRIMER_RIGHT_17_PENALTY=10.070442 PRIMER_LEFT_17_SEQUENCE=ACGGTGCTGTCCCTGTCTC PRIMER_RIGHT_17_SEQUENCE=CAGGCTACAGTGCTCACCGA PRIMER_LEFT_17=103,19 PRIMER_RIGHT_17=1033,20 PRIMER_LEFT_17_TM=61.322 PRIMER_RIGHT_17_TM=62.570 PRIMER_LEFT_17_GC_PERCENT=63.158 PRIMER_RIGHT_17_GC_PERCENT=60.000 PRIMER_LEFT_17_SELF_ANY=3.00 PRIMER_RIGHT_17_SELF_ANY=4.00 PRIMER_LEFT_17_SELF_END=0.00 PRIMER_RIGHT_17_SELF_END=2.00 PRIMER_LEFT_17_POSITION_PENALTY=120.000000 PRIMER_RIGHT_17_POSITION_PENALTY=7.500000 PRIMER_LEFT_17_END_STABILITY=6.1000 PRIMER_RIGHT_17_END_STABILITY=9.6000 PRIMER_PAIR_17_COMPL_ANY=5.00 PRIMER_PAIR_17_COMPL_END=3.00 PRIMER_PRODUCT_SIZE_17=931 PRIMER_PAIR_PENALTY_18=132.8596 PRIMER_LEFT_18_PENALTY=124.973289 PRIMER_RIGHT_18_PENALTY=7.886276 PRIMER_LEFT_18_SEQUENCE=ACGGTGCTGTCCCTGTCTCC PRIMER_RIGHT_18_SEQUENCE=CAGTGCTCACCGATCCAGG PRIMER_LEFT_18=103,20 PRIMER_RIGHT_18=1026,19 PRIMER_LEFT_18_TM=64.973 PRIMER_RIGHT_18_TM=62.886 PRIMER_LEFT_18_GC_PERCENT=65.000 PRIMER_RIGHT_18_GC_PERCENT=63.158 PRIMER_LEFT_18_SELF_ANY=3.00 PRIMER_RIGHT_18_SELF_ANY=4.00 PRIMER_LEFT_18_SELF_END=0.00 PRIMER_RIGHT_18_SELF_END=3.00 PRIMER_LEFT_18_POSITION_PENALTY=120.000000 PRIMER_RIGHT_18_POSITION_PENALTY=4.000000 PRIMER_LEFT_18_END_STABILITY=7.9000 PRIMER_RIGHT_18_END_STABILITY=9.7000 PRIMER_PAIR_18_COMPL_ANY=5.00 PRIMER_PAIR_18_COMPL_END=3.00 PRIMER_PRODUCT_SIZE_18=924 PRIMER_PAIR_PENALTY_19=132.9859 PRIMER_LEFT_19_PENALTY=122.322207 PRIMER_RIGHT_19_PENALTY=10.663709 PRIMER_LEFT_19_SEQUENCE=ACGGTGCTGTCCCTGTCTC PRIMER_RIGHT_19_SEQUENCE=GCTACAGTGCTCACCGATCCA PRIMER_LEFT_19=103,19 PRIMER_RIGHT_19=1030,21 PRIMER_LEFT_19_TM=61.322 PRIMER_RIGHT_19_TM=63.664 PRIMER_LEFT_19_GC_PERCENT=63.158 PRIMER_RIGHT_19_GC_PERCENT=57.143 PRIMER_LEFT_19_SELF_ANY=3.00 PRIMER_RIGHT_19_SELF_ANY=4.00 PRIMER_LEFT_19_SELF_END=0.00 PRIMER_RIGHT_19_SELF_END=0.00 PRIMER_LEFT_19_POSITION_PENALTY=120.000000 PRIMER_RIGHT_19_POSITION_PENALTY=6.000000 PRIMER_LEFT_19_END_STABILITY=6.1000 PRIMER_RIGHT_19_END_STABILITY=8.1000 PRIMER_PAIR_19_COMPL_ANY=5.00 PRIMER_PAIR_19_COMPL_END=3.00 PRIMER_PRODUCT_SIZE_19=928 = PRIMER_SEQUENCE_ID=L23959/DP1 PRIMER_COMMENT=Homo sapiens E2F-related transcription factor (DP-1) mRNA, complete cds. CDS=38..1270 PRIMER_START_CODON_POSITION=37 SEQUENCE=GGAATTCCGTAGCTATTGATTTCCCGGATCTGGTAACATGGCAAAAGATGCCGGTCTAATTGAAGCCAACGGAGAACTCAAGGTCTTCATAGACCAGAACCTTAGTCCCGGGAAAGGCGTGGTGTCCCTCGTGGCCGTTCACCCCTCCACCGTCAACCCGCTCGGGAAGCAGCTCTTGCCAAAAACCTTTGGACAGTCCAATGTCAACATTGCCCAGCAAGTGGTAATTGGTACGCCTCAGAGACCGGCAGCGTCAAACACCCTGGTGGTAGGAAGCCCACACACCCCCAGCACTCACTTTGCCTCTCAGAACCAGCCTTCCGACTCCTCACCTTGGTCTGCCGGGAAGCGCAACAGGAAAGGAGAGAAGAATGGCAAGGGCCTACGGCATTTCTCCATGAAGGTCTGCGAGAAGGTGCAGAGGAAAGGGACCACTTCCTACAACGAAGTGGCAGACGAGCTGGTTGCGGAGTTCAGTGCTGCCGACAACCACATCTTACCAAACGAGTCAGCTTATGACCAGAAAAACATAAGACGGCGCGTCTACGATGCCTTAAACGTGCTAATGGCCATGAACATCATCTCCAAGGAGAAGAAGGAGATCAAGTGGATTGGTCTGCCCACCAACTCGGCTCAGGAATGTCAGAACTTAGAGGTGGAAAGACAGAGGAGACTTGAAAGAATAAAACAGAAACAGTCTCAACTTCAAGAACTTATTCTACAGCAAATTGCCTTCAAGAACCTGGTGCAGAGAAACCGGCATGCGGAGCAGCAGGCCAGCCGGCCACCGCCACCCAACTCAGTCATCCACCTGCCCTTCATCATCGTCAACACCAGCAAGAAGACGGTCATCGACTGCAGCATCTCCAATGACAAATTTGAGTATCTGTTTAATTTTGACAACACATTTGAAATCCACGATGACATAGAAGTGCTGAAGCGGATGGGCATGGCTTGCGGGCTGGAGTCGGGGAGCTGCTCTGCCGAAGACCTTAAAATGGCCAGAAGTCTGGTCCCCAAGGCTCTGGAGCCATACGTGACAGAAATGGCTCAGGGAACTGTTGGAGGCGTGTTCATCACGACGGCAGGTTCCACGTCTAACGGCACAAGGTTCTCTGCCAGTGACCTGACCAACGGTGCAGATGGGATGCTGGCCACAAGCTCCAATGGGTCTCAGTACAGCGGCTCCAGGGTGGAGACTCCGGTGTCCTACGTCGGGGAGGACGACGAGGAGGACGATGACTTCAACGAGAATGACGAGGACGACTGACGTCCTCCCCACTTCAGATTCGGCTTCAGGAAAACGTTTAGCGAAAAGAAACTTTTTTTTTAATGTGGGTTTTCTGTTTCCTTTTGGCCTAGTCCCAAGAAGATATTGGTAAGCTATTGAATTTAGATATGCACCTCTGATAAGCAAGGATTGTTTCCCGTAGATTAGGA PRIMER_LEFT_EXPLAIN=considered 11963, would not amplify any of the ORF 8309, GC content failed 29, low tm 476, high tm 2059, high 3' stability 47, ok 1043 PRIMER_RIGHT_EXPLAIN=considered 11769, GC content failed 99, low tm 1773, high tm 6322, long poly-x seq 25, high 3' stability 49, ok 3501 PRIMER_PAIR_EXPLAIN=considered 23, ok 23 PRIMER_STOP_CODON_POSITION=1267 PRIMER_PAIR_PENALTY=4.4475 PRIMER_LEFT_PENALTY=4.039125 PRIMER_RIGHT_PENALTY=0.408401 PRIMER_LEFT_SEQUENCE=ATGGCAAAAGATGCCGGTCT PRIMER_RIGHT_SEQUENCE=AGTCGTCCTCGTCATTCTCG PRIMER_LEFT=37,20 PRIMER_RIGHT=1267,20 PRIMER_LEFT_TM=64.039 PRIMER_RIGHT_TM=60.408 PRIMER_LEFT_GC_PERCENT=50.000 PRIMER_RIGHT_GC_PERCENT=55.000 PRIMER_LEFT_SELF_ANY=4.00 PRIMER_RIGHT_SELF_ANY=2.00 PRIMER_LEFT_SELF_END=2.00 PRIMER_RIGHT_SELF_END=2.00 PRIMER_LEFT_POSITION_PENALTY=0.000000 PRIMER_RIGHT_POSITION_PENALTY=0.000000 PRIMER_LEFT_END_STABILITY=7.6000 PRIMER_RIGHT_END_STABILITY=8.4000 PRIMER_PAIR_COMPL_ANY=4.00 PRIMER_PAIR_COMPL_END=0.00 PRIMER_PRODUCT_SIZE=1231 PRIMER_PAIR_PENALTY_1=4.6661 PRIMER_LEFT_1_PENALTY=4.257663 PRIMER_RIGHT_1_PENALTY=0.408401 PRIMER_LEFT_1_SEQUENCE=ATGGCAAAAGATGCCGGTC PRIMER_RIGHT_1_SEQUENCE=AGTCGTCCTCGTCATTCTCG PRIMER_LEFT_1=37,19 PRIMER_RIGHT_1=1267,20 PRIMER_LEFT_1_TM=63.258 PRIMER_RIGHT_1_TM=60.408 PRIMER_LEFT_1_GC_PERCENT=52.632 PRIMER_RIGHT_1_GC_PERCENT=55.000 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=2.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_LEFT_1_POSITION_PENALTY=0.000000 PRIMER_RIGHT_1_POSITION_PENALTY=0.000000 PRIMER_LEFT_1_END_STABILITY=9.6000 PRIMER_RIGHT_1_END_STABILITY=8.4000 PRIMER_PAIR_1_COMPL_ANY=4.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_1=1231 PRIMER_PAIR_PENALTY_2=5.3122 PRIMER_LEFT_2_PENALTY=4.903833 PRIMER_RIGHT_2_PENALTY=0.408401 PRIMER_LEFT_2_SEQUENCE=ATGGCAAAAGATGCCGGTCTA PRIMER_RIGHT_2_SEQUENCE=AGTCGTCCTCGTCATTCTCG PRIMER_LEFT_2=37,21 PRIMER_RIGHT_2=1267,20 PRIMER_LEFT_2_TM=63.904 PRIMER_RIGHT_2_TM=60.408 PRIMER_LEFT_2_GC_PERCENT=47.619 PRIMER_RIGHT_2_GC_PERCENT=55.000 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=2.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_LEFT_2_POSITION_PENALTY=0.000000 PRIMER_RIGHT_2_POSITION_PENALTY=0.000000 PRIMER_LEFT_2_END_STABILITY=5.4000 PRIMER_RIGHT_2_END_STABILITY=8.4000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_2=1231 PRIMER_PAIR_PENALTY_3=6.1605 PRIMER_LEFT_3_PENALTY=4.039125 PRIMER_RIGHT_3_PENALTY=2.121401 PRIMER_LEFT_3_SEQUENCE=ATGGCAAAAGATGCCGGTCT PRIMER_RIGHT_3_SEQUENCE=CAGTCGTCCTCGTCATTCTC PRIMER_LEFT_3=37,20 PRIMER_RIGHT_3=1268,20 PRIMER_LEFT_3_TM=64.039 PRIMER_RIGHT_3_TM=58.379 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=55.000 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=2.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=0.00 PRIMER_LEFT_3_POSITION_PENALTY=0.000000 PRIMER_RIGHT_3_POSITION_PENALTY=0.500000 PRIMER_LEFT_3_END_STABILITY=7.6000 PRIMER_RIGHT_3_END_STABILITY=6.7000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_3=1232 PRIMER_PAIR_PENALTY_4=6.2743 PRIMER_LEFT_4_PENALTY=4.039125 PRIMER_RIGHT_4_PENALTY=2.235177 PRIMER_LEFT_4_SEQUENCE=ATGGCAAAAGATGCCGGTCT PRIMER_RIGHT_4_SEQUENCE=AGTCGTCCTCGTCATTCTCGT PRIMER_LEFT_4=37,20 PRIMER_RIGHT_4=1267,21 PRIMER_LEFT_4_TM=64.039 PRIMER_RIGHT_4_TM=61.235 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=52.381 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=2.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_LEFT_4_POSITION_PENALTY=0.000000 PRIMER_RIGHT_4_POSITION_PENALTY=0.000000 PRIMER_LEFT_4_END_STABILITY=7.6000 PRIMER_RIGHT_4_END_STABILITY=8.1000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_4=1231 PRIMER_PAIR_PENALTY_5=6.3791 PRIMER_LEFT_5_PENALTY=4.257663 PRIMER_RIGHT_5_PENALTY=2.121401 PRIMER_LEFT_5_SEQUENCE=ATGGCAAAAGATGCCGGTC PRIMER_RIGHT_5_SEQUENCE=CAGTCGTCCTCGTCATTCTC PRIMER_LEFT_5=37,19 PRIMER_RIGHT_5=1268,20 PRIMER_LEFT_5_TM=63.258 PRIMER_RIGHT_5_TM=58.379 PRIMER_LEFT_5_GC_PERCENT=52.632 PRIMER_RIGHT_5_GC_PERCENT=55.000 PRIMER_LEFT_5_SELF_ANY=4.00 PRIMER_RIGHT_5_SELF_ANY=2.00 PRIMER_LEFT_5_SELF_END=2.00 PRIMER_RIGHT_5_SELF_END=0.00 PRIMER_LEFT_5_POSITION_PENALTY=0.000000 PRIMER_RIGHT_5_POSITION_PENALTY=0.500000 PRIMER_LEFT_5_END_STABILITY=9.6000 PRIMER_RIGHT_5_END_STABILITY=6.7000 PRIMER_PAIR_5_COMPL_ANY=4.00 PRIMER_PAIR_5_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_5=1232 PRIMER_PAIR_PENALTY_6=6.4408 PRIMER_LEFT_6_PENALTY=4.039125 PRIMER_RIGHT_6_PENALTY=2.401642 PRIMER_LEFT_6_SEQUENCE=ATGGCAAAAGATGCCGGTCT PRIMER_RIGHT_6_SEQUENCE=TCAGTCGTCCTCGTCATTCTC PRIMER_LEFT_6=37,20 PRIMER_RIGHT_6=1269,21 PRIMER_LEFT_6_TM=64.039 PRIMER_RIGHT_6_TM=60.402 PRIMER_LEFT_6_GC_PERCENT=50.000 PRIMER_RIGHT_6_GC_PERCENT=52.381 PRIMER_LEFT_6_SELF_ANY=4.00 PRIMER_RIGHT_6_SELF_ANY=2.00 PRIMER_LEFT_6_SELF_END=2.00 PRIMER_RIGHT_6_SELF_END=0.00 PRIMER_LEFT_6_POSITION_PENALTY=0.000000 PRIMER_RIGHT_6_POSITION_PENALTY=1.000000 PRIMER_LEFT_6_END_STABILITY=7.6000 PRIMER_RIGHT_6_END_STABILITY=6.7000 PRIMER_PAIR_6_COMPL_ANY=4.00 PRIMER_PAIR_6_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_6=1233 PRIMER_PAIR_PENALTY_7=6.4928 PRIMER_LEFT_7_PENALTY=4.257663 PRIMER_RIGHT_7_PENALTY=2.235177 PRIMER_LEFT_7_SEQUENCE=ATGGCAAAAGATGCCGGTC PRIMER_RIGHT_7_SEQUENCE=AGTCGTCCTCGTCATTCTCGT PRIMER_LEFT_7=37,19 PRIMER_RIGHT_7=1267,21 PRIMER_LEFT_7_TM=63.258 PRIMER_RIGHT_7_TM=61.235 PRIMER_LEFT_7_GC_PERCENT=52.632 PRIMER_RIGHT_7_GC_PERCENT=52.381 PRIMER_LEFT_7_SELF_ANY=4.00 PRIMER_RIGHT_7_SELF_ANY=2.00 PRIMER_LEFT_7_SELF_END=2.00 PRIMER_RIGHT_7_SELF_END=0.00 PRIMER_LEFT_7_POSITION_PENALTY=0.000000 PRIMER_RIGHT_7_POSITION_PENALTY=0.000000 PRIMER_LEFT_7_END_STABILITY=9.6000 PRIMER_RIGHT_7_END_STABILITY=8.1000 PRIMER_PAIR_7_COMPL_ANY=4.00 PRIMER_PAIR_7_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_7=1231 PRIMER_PAIR_PENALTY_8=6.6593 PRIMER_LEFT_8_PENALTY=4.257663 PRIMER_RIGHT_8_PENALTY=2.401642 PRIMER_LEFT_8_SEQUENCE=ATGGCAAAAGATGCCGGTC PRIMER_RIGHT_8_SEQUENCE=TCAGTCGTCCTCGTCATTCTC PRIMER_LEFT_8=37,19 PRIMER_RIGHT_8=1269,21 PRIMER_LEFT_8_TM=63.258 PRIMER_RIGHT_8_TM=60.402 PRIMER_LEFT_8_GC_PERCENT=52.632 PRIMER_RIGHT_8_GC_PERCENT=52.381 PRIMER_LEFT_8_SELF_ANY=4.00 PRIMER_RIGHT_8_SELF_ANY=2.00 PRIMER_LEFT_8_SELF_END=2.00 PRIMER_RIGHT_8_SELF_END=0.00 PRIMER_LEFT_8_POSITION_PENALTY=0.000000 PRIMER_RIGHT_8_POSITION_PENALTY=1.000000 PRIMER_LEFT_8_END_STABILITY=9.6000 PRIMER_RIGHT_8_END_STABILITY=6.7000 PRIMER_PAIR_8_COMPL_ANY=4.00 PRIMER_PAIR_8_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_8=1233 PRIMER_PAIR_PENALTY_9=6.6605 PRIMER_LEFT_9_PENALTY=4.039125 PRIMER_RIGHT_9_PENALTY=2.621401 PRIMER_LEFT_9_SEQUENCE=ATGGCAAAAGATGCCGGTCT PRIMER_RIGHT_9_SEQUENCE=TCAGTCGTCCTCGTCATTCT PRIMER_LEFT_9=37,20 PRIMER_RIGHT_9=1269,20 PRIMER_LEFT_9_TM=64.039 PRIMER_RIGHT_9_TM=58.379 PRIMER_LEFT_9_GC_PERCENT=50.000 PRIMER_RIGHT_9_GC_PERCENT=50.000 PRIMER_LEFT_9_SELF_ANY=4.00 PRIMER_RIGHT_9_SELF_ANY=2.00 PRIMER_LEFT_9_SELF_END=2.00 PRIMER_RIGHT_9_SELF_END=0.00 PRIMER_LEFT_9_POSITION_PENALTY=0.000000 PRIMER_RIGHT_9_POSITION_PENALTY=1.000000 PRIMER_LEFT_9_END_STABILITY=7.6000 PRIMER_RIGHT_9_END_STABILITY=6.6000 PRIMER_PAIR_9_COMPL_ANY=4.00 PRIMER_PAIR_9_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_9=1233 PRIMER_PAIR_PENALTY_10=6.8791 PRIMER_LEFT_10_PENALTY=4.257663 PRIMER_RIGHT_10_PENALTY=2.621401 PRIMER_LEFT_10_SEQUENCE=ATGGCAAAAGATGCCGGTC PRIMER_RIGHT_10_SEQUENCE=TCAGTCGTCCTCGTCATTCT PRIMER_LEFT_10=37,19 PRIMER_RIGHT_10=1269,20 PRIMER_LEFT_10_TM=63.258 PRIMER_RIGHT_10_TM=58.379 PRIMER_LEFT_10_GC_PERCENT=52.632 PRIMER_RIGHT_10_GC_PERCENT=50.000 PRIMER_LEFT_10_SELF_ANY=4.00 PRIMER_RIGHT_10_SELF_ANY=2.00 PRIMER_LEFT_10_SELF_END=2.00 PRIMER_RIGHT_10_SELF_END=0.00 PRIMER_LEFT_10_POSITION_PENALTY=0.000000 PRIMER_RIGHT_10_POSITION_PENALTY=1.000000 PRIMER_LEFT_10_END_STABILITY=9.6000 PRIMER_RIGHT_10_END_STABILITY=6.6000 PRIMER_PAIR_10_COMPL_ANY=4.00 PRIMER_PAIR_10_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_10=1233 PRIMER_PAIR_PENALTY_11=7.0252 PRIMER_LEFT_11_PENALTY=4.903833 PRIMER_RIGHT_11_PENALTY=2.121401 PRIMER_LEFT_11_SEQUENCE=ATGGCAAAAGATGCCGGTCTA PRIMER_RIGHT_11_SEQUENCE=CAGTCGTCCTCGTCATTCTC PRIMER_LEFT_11=37,21 PRIMER_RIGHT_11=1268,20 PRIMER_LEFT_11_TM=63.904 PRIMER_RIGHT_11_TM=58.379 PRIMER_LEFT_11_GC_PERCENT=47.619 PRIMER_RIGHT_11_GC_PERCENT=55.000 PRIMER_LEFT_11_SELF_ANY=4.00 PRIMER_RIGHT_11_SELF_ANY=2.00 PRIMER_LEFT_11_SELF_END=2.00 PRIMER_RIGHT_11_SELF_END=0.00 PRIMER_LEFT_11_POSITION_PENALTY=0.000000 PRIMER_RIGHT_11_POSITION_PENALTY=0.500000 PRIMER_LEFT_11_END_STABILITY=5.4000 PRIMER_RIGHT_11_END_STABILITY=6.7000 PRIMER_PAIR_11_COMPL_ANY=4.00 PRIMER_PAIR_11_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_11=1232 PRIMER_PAIR_PENALTY_12=7.1390 PRIMER_LEFT_12_PENALTY=4.903833 PRIMER_RIGHT_12_PENALTY=2.235177 PRIMER_LEFT_12_SEQUENCE=ATGGCAAAAGATGCCGGTCTA PRIMER_RIGHT_12_SEQUENCE=AGTCGTCCTCGTCATTCTCGT PRIMER_LEFT_12=37,21 PRIMER_RIGHT_12=1267,21 PRIMER_LEFT_12_TM=63.904 PRIMER_RIGHT_12_TM=61.235 PRIMER_LEFT_12_GC_PERCENT=47.619 PRIMER_RIGHT_12_GC_PERCENT=52.381 PRIMER_LEFT_12_SELF_ANY=4.00 PRIMER_RIGHT_12_SELF_ANY=2.00 PRIMER_LEFT_12_SELF_END=2.00 PRIMER_RIGHT_12_SELF_END=0.00 PRIMER_LEFT_12_POSITION_PENALTY=0.000000 PRIMER_RIGHT_12_POSITION_PENALTY=0.000000 PRIMER_LEFT_12_END_STABILITY=5.4000 PRIMER_RIGHT_12_END_STABILITY=8.1000 PRIMER_PAIR_12_COMPL_ANY=4.00 PRIMER_PAIR_12_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_12=1231 PRIMER_PAIR_PENALTY_13=7.2026 PRIMER_LEFT_13_PENALTY=4.039125 PRIMER_RIGHT_13_PENALTY=3.163428 PRIMER_LEFT_13_SEQUENCE=ATGGCAAAAGATGCCGGTCT PRIMER_RIGHT_13_SEQUENCE=CGTCAGTCGTCCTCGTCAT PRIMER_LEFT_13=37,20 PRIMER_RIGHT_13=1271,19 PRIMER_LEFT_13_TM=64.039 PRIMER_RIGHT_13_TM=59.837 PRIMER_LEFT_13_GC_PERCENT=50.000 PRIMER_RIGHT_13_GC_PERCENT=57.895 PRIMER_LEFT_13_SELF_ANY=4.00 PRIMER_RIGHT_13_SELF_ANY=3.00 PRIMER_LEFT_13_SELF_END=2.00 PRIMER_RIGHT_13_SELF_END=2.00 PRIMER_LEFT_13_POSITION_PENALTY=0.000000 PRIMER_RIGHT_13_POSITION_PENALTY=2.000000 PRIMER_LEFT_13_END_STABILITY=7.6000 PRIMER_RIGHT_13_END_STABILITY=6.3000 PRIMER_PAIR_13_COMPL_ANY=4.00 PRIMER_PAIR_13_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_13=1235 PRIMER_PAIR_PENALTY_14=7.2196 PRIMER_LEFT_14_PENALTY=4.039125 PRIMER_RIGHT_14_PENALTY=3.180452 PRIMER_LEFT_14_SEQUENCE=ATGGCAAAAGATGCCGGTCT PRIMER_RIGHT_14_SEQUENCE=GTCAGTCGTCCTCGTCATTCT PRIMER_LEFT_14=37,20 PRIMER_RIGHT_14=1270,21 PRIMER_LEFT_14_TM=64.039 PRIMER_RIGHT_14_TM=59.320 PRIMER_LEFT_14_GC_PERCENT=50.000 PRIMER_RIGHT_14_GC_PERCENT=52.381 PRIMER_LEFT_14_SELF_ANY=4.00 PRIMER_RIGHT_14_SELF_ANY=2.00 PRIMER_LEFT_14_SELF_END=2.00 PRIMER_RIGHT_14_SELF_END=0.00 PRIMER_LEFT_14_POSITION_PENALTY=0.000000 PRIMER_RIGHT_14_POSITION_PENALTY=1.500000 PRIMER_LEFT_14_END_STABILITY=7.6000 PRIMER_RIGHT_14_END_STABILITY=6.6000 PRIMER_PAIR_14_COMPL_ANY=4.00 PRIMER_PAIR_14_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_14=1234 PRIMER_PAIR_PENALTY_15=7.2848 PRIMER_LEFT_15_PENALTY=4.039125 PRIMER_RIGHT_15_PENALTY=3.245695 PRIMER_LEFT_15_SEQUENCE=ATGGCAAAAGATGCCGGTCT PRIMER_RIGHT_15_SEQUENCE=ACGTCAGTCGTCCTCGTCAT PRIMER_LEFT_15=37,20 PRIMER_RIGHT_15=1272,20 PRIMER_LEFT_15_TM=64.039 PRIMER_RIGHT_15_TM=60.746 PRIMER_LEFT_15_GC_PERCENT=50.000 PRIMER_RIGHT_15_GC_PERCENT=55.000 PRIMER_LEFT_15_SELF_ANY=4.00 PRIMER_RIGHT_15_SELF_ANY=5.00 PRIMER_LEFT_15_SELF_END=2.00 PRIMER_RIGHT_15_SELF_END=2.00 PRIMER_LEFT_15_POSITION_PENALTY=0.000000 PRIMER_RIGHT_15_POSITION_PENALTY=2.500000 PRIMER_LEFT_15_END_STABILITY=7.6000 PRIMER_RIGHT_15_END_STABILITY=6.3000 PRIMER_PAIR_15_COMPL_ANY=4.00 PRIMER_PAIR_15_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_15=1236 PRIMER_PAIR_PENALTY_16=7.3055 PRIMER_LEFT_16_PENALTY=4.903833 PRIMER_RIGHT_16_PENALTY=2.401642 PRIMER_LEFT_16_SEQUENCE=ATGGCAAAAGATGCCGGTCTA PRIMER_RIGHT_16_SEQUENCE=TCAGTCGTCCTCGTCATTCTC PRIMER_LEFT_16=37,21 PRIMER_RIGHT_16=1269,21 PRIMER_LEFT_16_TM=63.904 PRIMER_RIGHT_16_TM=60.402 PRIMER_LEFT_16_GC_PERCENT=47.619 PRIMER_RIGHT_16_GC_PERCENT=52.381 PRIMER_LEFT_16_SELF_ANY=4.00 PRIMER_RIGHT_16_SELF_ANY=2.00 PRIMER_LEFT_16_SELF_END=2.00 PRIMER_RIGHT_16_SELF_END=0.00 PRIMER_LEFT_16_POSITION_PENALTY=0.000000 PRIMER_RIGHT_16_POSITION_PENALTY=1.000000 PRIMER_LEFT_16_END_STABILITY=5.4000 PRIMER_RIGHT_16_END_STABILITY=6.7000 PRIMER_PAIR_16_COMPL_ANY=4.00 PRIMER_PAIR_16_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_16=1233 PRIMER_PAIR_PENALTY_17=7.3104 PRIMER_LEFT_17_PENALTY=6.902047 PRIMER_RIGHT_17_PENALTY=0.408401 PRIMER_LEFT_17_SEQUENCE=ATGGCAAAAGATGCCGGTCTAA PRIMER_RIGHT_17_SEQUENCE=AGTCGTCCTCGTCATTCTCG PRIMER_LEFT_17=37,22 PRIMER_RIGHT_17=1267,20 PRIMER_LEFT_17_TM=64.902 PRIMER_RIGHT_17_TM=60.408 PRIMER_LEFT_17_GC_PERCENT=45.455 PRIMER_RIGHT_17_GC_PERCENT=55.000 PRIMER_LEFT_17_SELF_ANY=4.00 PRIMER_RIGHT_17_SELF_ANY=2.00 PRIMER_LEFT_17_SELF_END=1.00 PRIMER_RIGHT_17_SELF_END=2.00 PRIMER_LEFT_17_POSITION_PENALTY=0.000000 PRIMER_RIGHT_17_POSITION_PENALTY=0.000000 PRIMER_LEFT_17_END_STABILITY=6.0000 PRIMER_RIGHT_17_END_STABILITY=8.4000 PRIMER_PAIR_17_COMPL_ANY=4.00 PRIMER_PAIR_17_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_17=1231 PRIMER_PAIR_PENALTY_18=7.3184 PRIMER_LEFT_18_PENALTY=4.039125 PRIMER_RIGHT_18_PENALTY=3.279241 PRIMER_LEFT_18_SEQUENCE=ATGGCAAAAGATGCCGGTCT PRIMER_RIGHT_18_SEQUENCE=CGTCAGTCGTCCTCGTCATT PRIMER_LEFT_18=37,20 PRIMER_RIGHT_18=1271,20 PRIMER_LEFT_18_TM=64.039 PRIMER_RIGHT_18_TM=61.279 PRIMER_LEFT_18_GC_PERCENT=50.000 PRIMER_RIGHT_18_GC_PERCENT=55.000 PRIMER_LEFT_18_SELF_ANY=4.00 PRIMER_RIGHT_18_SELF_ANY=3.00 PRIMER_LEFT_18_SELF_END=2.00 PRIMER_RIGHT_18_SELF_END=1.00 PRIMER_LEFT_18_POSITION_PENALTY=0.000000 PRIMER_RIGHT_18_POSITION_PENALTY=2.000000 PRIMER_LEFT_18_END_STABILITY=7.6000 PRIMER_RIGHT_18_END_STABILITY=6.9000 PRIMER_PAIR_18_COMPL_ANY=4.00 PRIMER_PAIR_18_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_18=1235 PRIMER_PAIR_PENALTY_19=7.3228 PRIMER_LEFT_19_PENALTY=4.039125 PRIMER_RIGHT_19_PENALTY=3.283719 PRIMER_LEFT_19_SEQUENCE=ATGGCAAAAGATGCCGGTCT PRIMER_RIGHT_19_SEQUENCE=GTCAGTCGTCCTCGTCATTC PRIMER_LEFT_19=37,20 PRIMER_RIGHT_19=1270,20 PRIMER_LEFT_19_TM=64.039 PRIMER_RIGHT_19_TM=58.216 PRIMER_LEFT_19_GC_PERCENT=50.000 PRIMER_RIGHT_19_GC_PERCENT=55.000 PRIMER_LEFT_19_SELF_ANY=4.00 PRIMER_RIGHT_19_SELF_ANY=2.00 PRIMER_LEFT_19_SELF_END=2.00 PRIMER_RIGHT_19_SELF_END=0.00 PRIMER_LEFT_19_POSITION_PENALTY=0.000000 PRIMER_RIGHT_19_POSITION_PENALTY=1.500000 PRIMER_LEFT_19_END_STABILITY=7.6000 PRIMER_RIGHT_19_END_STABILITY=6.9000 PRIMER_PAIR_19_COMPL_ANY=4.00 PRIMER_PAIR_19_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_19=1234 = PRIMER_SEQUENCE_ID=X98032/E6AP PRIMER_COMMENT=H.sapiens mRNA for E6-AP isoform-I. CDS=1..2559 PRIMER_START_CODON_POSITION=0 SEQUENCE=ATGAAGCGAGCAGCTGCAAAGCATCTAATAGAACGCTACTACCACCAGTTAACTGAGGGCTGTGGAAATGAAGCCTGCACGAATGAGTTTTGTGCTTCCTGTCCAACTTTTCTTCGTATGGATAATAATGCAGCAGCTATTAAAGCCCTCGAGCTTTATAAGATTAATGCAAAACTCTGTGATCCTCATCCCTCCAAGAAAGGAGCAAGCTCAGCTTACCTTGAGAACTCGAAAGGTGCCCCCAACAACTCCTGCTCTGAGATAAAAATGAACAAGAAAGGCGCTAGAATTGATTTTAAAGATGTGACTTACTTAACAGAAGAGAAGGTATATGAAATTCTTGAATTATGTAGAGAAAGAGAGGATTATTCCCCTTTAATCCGTGTTATTGGAAGAGTTTTTTCTAGTGCTGAGGCATTGGTACAGAGCTTCCGGAAAGTTAAACAACACACCAAGGAAGAACTGAAATCTCTTCAAGCAAAAGATGAAGACAAAGATGAAGATGAAAAGGAAAAAGCTGCATGTTCTGCTGCTGCTATGGAAGAAGACTCAGAAGCATCTTCCTCAAGGATAGGTGATAGCTCACAGGGAGACAACAATTTGCAAAAATTAGGCCCTGATGATGTGTCTGTGGATATTGATGCCATTAGAAGGGTCTACACCAGATTGCTCTCTAATGAAAAAATTGAAACTGCCTTTCTCAATGCACTTGTATATTTGTCACCTAACGTGGAATGTGACTTGACGTATCACAATGTATACTCTCGAGATCCTAATTATCTGAATTTGTTCATTATCGGAATGGAGAATAGAAATCTCCACAGTCCTGAATATCTGGAAATGGCTTTGCCATTATTTTGCAAAGCGATGAGCAAGCTACCCCTTGCAGCCCAAGGAAAACTGATCAGACTGTGGTCTAAATACAATGCAGACCAGATTCGGAGAATGATGGAGACATTTCAGCAACTTATTACTTATAAAGTCATAAGCAATGAATTTAACAGTCGAAATCTAGTGAATGATGATGATGCCATTGTTGCTGCTTCGAAGTGCTTGAAAATGGTTTACTATGCAAATGTAGTGGGAGGGGAAGTGGACACAAATCACAATGAAGAAGATGATGAAGAGCCCATCCCTGAGTCCAGCGAGCTGACACTTCAGGAACTTTTGGGAGAAGAAAGAAGAAACAAGAAAGGTCCTCGAGTGGACCCCCTGGAAACTGAACTTGGTGTTAAAACCCTGGATTGTCGAAAACCACTTATCCCTTTTGAAGAGTTTATTAATGAACCACTGAATGAGGTTCTAGAAATGGATAAAGATTATACTTTTTTCAAAGTAGAAACAGAGAACAAATTCTCTTTTATGACATGTCCCTTTATATTGAATGCTGTCACAAAGAATTTGGGATTATATTATGACAATAGAATTCGCATGTACAGTGAACGAAGAATCACTGTTCTCTACAGCTTAGTTCAAGGACAGCAGTTGAATCCATATTTGAGACTCAAAGTTAGACGTGACCATATCATAGATGATGCACTTGTCCGGCTAGAGATGATCGCTATGGAAAATCCTGCAGACTTGAAGAAGCAGTTGTATGTGGAATTTGAAGGAGAACAAGGAGTTGATGAGGGAGGTGTTTCCAAAGAATTTTTTCAGCTGGTTGTGGAGGAAATCTTCAATCCAGATATTGGTATGTTCACATACGATGAATCTACAAAATTGTTTTGGTTTAATCCATCTTCTTTTGAAACTGAGGGTCAGTTTACTCTGATTGGCATAGTACTGGGTCTGGCTATTTACAATAACTGTATACTGGATGTACATTTTCCCATGGTTGTCTACAGGAAGCTAATGGGGAAAAAAGGAACTTTTCGTGACTTGGGAGACTCTCACCCAGTTCTATATCAGAGTTTAAAAGATTTATTGGAGTATGAAGGGAATGTGGAAGATGACATGATGATCACTTTCCAGATATCACAGACAGATCTTTTTGGTAACCCAATGATGTATGATCTAAAGGAAAATGGTGATAAAATTCCAATTACAAATGAAAACAGGAAGGAATTTGTCAATCTTTATTCTGACTACATTCTCAATAAATCAGTAGAAAAACAGTTCAAGGCTTTTCGGAGAGGTTTTCATATGGTGACCAATGAATCTCCCTTAAAGTACTTATTCAGACCAGAAGAAATTGAATTGCTTATATGTGGAAGCCGGAATCTAGATTTCCAAGCACTAGAAGAAACTACAGAATATGACGGTGGCTATACCAGGGACTCTGTTCTGATTAGGGAGTTCTGGGAAATCGTTCATTCATTTACAGATGAACAGAAAAGACTCTTCTTGCAGTTTACAACGGGCACAGACAGAGCACCTGTGGGAGGACTAGGAAAATTAAAGATGATTATAGCCAAAAATGGCCCAGACACAGAAAGGTTACCTACATCTCATACTTGCTTTAATGTGCTTTTACTTCCGGAATACTCAAGCAAAGAAAAACTTAAAGAGAGATTGTTGAAGGCCATCACGTATGCCAAAGGATTTGGCATGCTGTAA PRIMER_LEFT_EXPLAIN=considered 21507, would not amplify any of the ORF 14282, GC content failed 55, low tm 3071, high tm 1071, high end compl 6, long poly-x seq 102, high 3' stability 49, ok 2871 PRIMER_RIGHT_EXPLAIN=considered 21345, GC content failed 229, low tm 9108, high tm 3197, high end compl 7, long poly-x seq 106, high 3' stability 53, ok 8645 PRIMER_PAIR_EXPLAIN=considered 27, high end compl 6, ok 21 PRIMER_STOP_CODON_POSITION=2556 PRIMER_PAIR_PENALTY=6.7537 PRIMER_LEFT_PENALTY=4.494664 PRIMER_RIGHT_PENALTY=2.259083 PRIMER_LEFT_SEQUENCE=ATGAAGCGAGCAGCTGCAAA PRIMER_RIGHT_SEQUENCE=TACAGCATGCCAAATCCTTT PRIMER_LEFT=0,20 PRIMER_RIGHT=2557,20 PRIMER_LEFT_TM=64.495 PRIMER_RIGHT_TM=58.241 PRIMER_LEFT_GC_PERCENT=50.000 PRIMER_RIGHT_GC_PERCENT=40.000 PRIMER_LEFT_SELF_ANY=8.00 PRIMER_RIGHT_SELF_ANY=6.00 PRIMER_LEFT_SELF_END=2.00 PRIMER_RIGHT_SELF_END=3.00 PRIMER_LEFT_POSITION_PENALTY=0.000000 PRIMER_RIGHT_POSITION_PENALTY=0.500000 PRIMER_LEFT_END_STABILITY=8.8000 PRIMER_RIGHT_END_STABILITY=8.5000 PRIMER_PAIR_COMPL_ANY=5.00 PRIMER_PAIR_COMPL_END=3.00 PRIMER_PRODUCT_SIZE=2558 PRIMER_PAIR_PENALTY_1=6.8946 PRIMER_LEFT_1_PENALTY=4.494664 PRIMER_RIGHT_1_PENALTY=2.399914 PRIMER_LEFT_1_SEQUENCE=ATGAAGCGAGCAGCTGCAAA PRIMER_RIGHT_1_SEQUENCE=TTACAGCATGCCAAATCCTTT PRIMER_LEFT_1=0,20 PRIMER_RIGHT_1=2558,21 PRIMER_LEFT_1_TM=64.495 PRIMER_RIGHT_1_TM=59.600 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=38.095 PRIMER_LEFT_1_SELF_ANY=8.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_LEFT_1_POSITION_PENALTY=0.000000 PRIMER_RIGHT_1_POSITION_PENALTY=1.000000 PRIMER_LEFT_1_END_STABILITY=8.8000 PRIMER_RIGHT_1_END_STABILITY=8.5000 PRIMER_PAIR_1_COMPL_ANY=6.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PRODUCT_SIZE_1=2559 PRIMER_PAIR_PENALTY_2=7.2537 PRIMER_LEFT_2_PENALTY=4.494664 PRIMER_RIGHT_2_PENALTY=2.759083 PRIMER_LEFT_2_SEQUENCE=ATGAAGCGAGCAGCTGCAAA PRIMER_RIGHT_2_SEQUENCE=TTACAGCATGCCAAATCCTT PRIMER_LEFT_2=0,20 PRIMER_RIGHT_2=2558,20 PRIMER_LEFT_2_TM=64.495 PRIMER_RIGHT_2_TM=58.241 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=40.000 PRIMER_LEFT_2_SELF_ANY=8.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=1.00 PRIMER_LEFT_2_POSITION_PENALTY=0.000000 PRIMER_RIGHT_2_POSITION_PENALTY=1.000000 PRIMER_LEFT_2_END_STABILITY=8.8000 PRIMER_RIGHT_2_END_STABILITY=8.2000 PRIMER_PAIR_2_COMPL_ANY=6.00 PRIMER_PAIR_2_COMPL_END=3.00 PRIMER_PRODUCT_SIZE_2=2559 PRIMER_PAIR_PENALTY_3=7.3356 PRIMER_LEFT_3_PENALTY=4.494664 PRIMER_RIGHT_3_PENALTY=2.840968 PRIMER_LEFT_3_SEQUENCE=ATGAAGCGAGCAGCTGCAAA PRIMER_RIGHT_3_SEQUENCE=ACAGCATGCCAAATCCTTT PRIMER_LEFT_3=0,20 PRIMER_RIGHT_3=2556,19 PRIMER_LEFT_3_TM=64.495 PRIMER_RIGHT_3_TM=58.159 PRIMER_LEFT_3_GC_PERCENT=50.000 PRIMER_RIGHT_3_GC_PERCENT=42.105 PRIMER_LEFT_3_SELF_ANY=8.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=3.00 PRIMER_LEFT_3_POSITION_PENALTY=0.000000 PRIMER_RIGHT_3_POSITION_PENALTY=0.000000 PRIMER_LEFT_3_END_STABILITY=8.8000 PRIMER_RIGHT_3_END_STABILITY=8.5000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=3.00 PRIMER_PRODUCT_SIZE_3=2557 PRIMER_PAIR_PENALTY_4=127.1022 PRIMER_LEFT_4_PENALTY=124.343098 PRIMER_RIGHT_4_PENALTY=2.759083 PRIMER_LEFT_4_SEQUENCE=CGAGCAGCTGCAAAGCATC PRIMER_RIGHT_4_SEQUENCE=TTACAGCATGCCAAATCCTT PRIMER_LEFT_4=6,19 PRIMER_RIGHT_4=2558,20 PRIMER_LEFT_4_TM=63.343 PRIMER_RIGHT_4_TM=58.241 PRIMER_LEFT_4_GC_PERCENT=57.895 PRIMER_RIGHT_4_GC_PERCENT=40.000 PRIMER_LEFT_4_SELF_ANY=8.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=3.00 PRIMER_RIGHT_4_SELF_END=1.00 PRIMER_LEFT_4_POSITION_PENALTY=120.000000 PRIMER_RIGHT_4_POSITION_PENALTY=1.000000 PRIMER_LEFT_4_END_STABILITY=8.1000 PRIMER_RIGHT_4_END_STABILITY=8.2000 PRIMER_PAIR_4_COMPL_ANY=6.00 PRIMER_PAIR_4_COMPL_END=3.00 PRIMER_PRODUCT_SIZE_4=2553 PRIMER_PAIR_PENALTY_5=127.7645 PRIMER_LEFT_5_PENALTY=125.005410 PRIMER_RIGHT_5_PENALTY=2.759083 PRIMER_LEFT_5_SEQUENCE=CGAGCAGCTGCAAAGCATCTA PRIMER_RIGHT_5_SEQUENCE=TTACAGCATGCCAAATCCTT PRIMER_LEFT_5=6,21 PRIMER_RIGHT_5=2558,20 PRIMER_LEFT_5_TM=64.005 PRIMER_RIGHT_5_TM=58.241 PRIMER_LEFT_5_GC_PERCENT=52.381 PRIMER_RIGHT_5_GC_PERCENT=40.000 PRIMER_LEFT_5_SELF_ANY=8.00 PRIMER_RIGHT_5_SELF_ANY=6.00 PRIMER_LEFT_5_SELF_END=3.00 PRIMER_RIGHT_5_SELF_END=1.00 PRIMER_LEFT_5_POSITION_PENALTY=120.000000 PRIMER_RIGHT_5_POSITION_PENALTY=1.000000 PRIMER_LEFT_5_END_STABILITY=5.6000 PRIMER_RIGHT_5_END_STABILITY=8.2000 PRIMER_PAIR_5_COMPL_ANY=6.00 PRIMER_PAIR_5_COMPL_END=3.00 PRIMER_PRODUCT_SIZE_5=2553 PRIMER_PAIR_PENALTY_6=131.1391 PRIMER_LEFT_6_PENALTY=128.880021 PRIMER_RIGHT_6_PENALTY=2.259083 PRIMER_LEFT_6_SEQUENCE=CGAGCAGCTGCAAAGCATCTAATA PRIMER_RIGHT_6_SEQUENCE=TACAGCATGCCAAATCCTTT PRIMER_LEFT_6=6,24 PRIMER_RIGHT_6=2557,20 PRIMER_LEFT_6_TM=64.880 PRIMER_RIGHT_6_TM=58.241 PRIMER_LEFT_6_GC_PERCENT=45.833 PRIMER_RIGHT_6_GC_PERCENT=40.000 PRIMER_LEFT_6_SELF_ANY=8.00 PRIMER_RIGHT_6_SELF_ANY=6.00 PRIMER_LEFT_6_SELF_END=3.00 PRIMER_RIGHT_6_SELF_END=3.00 PRIMER_LEFT_6_POSITION_PENALTY=120.000000 PRIMER_RIGHT_6_POSITION_PENALTY=0.500000 PRIMER_LEFT_6_END_STABILITY=5.2000 PRIMER_RIGHT_6_END_STABILITY=8.5000 PRIMER_PAIR_6_COMPL_ANY=5.00 PRIMER_PAIR_6_COMPL_END=3.00 PRIMER_PRODUCT_SIZE_6=2552 PRIMER_PAIR_PENALTY_7=131.2799 PRIMER_LEFT_7_PENALTY=128.880021 PRIMER_RIGHT_7_PENALTY=2.399914 PRIMER_LEFT_7_SEQUENCE=CGAGCAGCTGCAAAGCATCTAATA PRIMER_RIGHT_7_SEQUENCE=TTACAGCATGCCAAATCCTTT PRIMER_LEFT_7=6,24 PRIMER_RIGHT_7=2558,21 PRIMER_LEFT_7_TM=64.880 PRIMER_RIGHT_7_TM=59.600 PRIMER_LEFT_7_GC_PERCENT=45.833 PRIMER_RIGHT_7_GC_PERCENT=38.095 PRIMER_LEFT_7_SELF_ANY=8.00 PRIMER_RIGHT_7_SELF_ANY=6.00 PRIMER_LEFT_7_SELF_END=3.00 PRIMER_RIGHT_7_SELF_END=3.00 PRIMER_LEFT_7_POSITION_PENALTY=120.000000 PRIMER_RIGHT_7_POSITION_PENALTY=1.000000 PRIMER_LEFT_7_END_STABILITY=5.2000 PRIMER_RIGHT_7_END_STABILITY=8.5000 PRIMER_PAIR_7_COMPL_ANY=6.00 PRIMER_PAIR_7_COMPL_END=3.00 PRIMER_PRODUCT_SIZE_7=2553 PRIMER_PAIR_PENALTY_8=131.6391 PRIMER_LEFT_8_PENALTY=128.880021 PRIMER_RIGHT_8_PENALTY=2.759083 PRIMER_LEFT_8_SEQUENCE=CGAGCAGCTGCAAAGCATCTAATA PRIMER_RIGHT_8_SEQUENCE=TTACAGCATGCCAAATCCTT PRIMER_LEFT_8=6,24 PRIMER_RIGHT_8=2558,20 PRIMER_LEFT_8_TM=64.880 PRIMER_RIGHT_8_TM=58.241 PRIMER_LEFT_8_GC_PERCENT=45.833 PRIMER_RIGHT_8_GC_PERCENT=40.000 PRIMER_LEFT_8_SELF_ANY=8.00 PRIMER_RIGHT_8_SELF_ANY=6.00 PRIMER_LEFT_8_SELF_END=3.00 PRIMER_RIGHT_8_SELF_END=1.00 PRIMER_LEFT_8_POSITION_PENALTY=120.000000 PRIMER_RIGHT_8_POSITION_PENALTY=1.000000 PRIMER_LEFT_8_END_STABILITY=5.2000 PRIMER_RIGHT_8_END_STABILITY=8.2000 PRIMER_PAIR_8_COMPL_ANY=6.00 PRIMER_PAIR_8_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_8=2553 PRIMER_PAIR_PENALTY_9=131.7210 PRIMER_LEFT_9_PENALTY=128.880021 PRIMER_RIGHT_9_PENALTY=2.840968 PRIMER_LEFT_9_SEQUENCE=CGAGCAGCTGCAAAGCATCTAATA PRIMER_RIGHT_9_SEQUENCE=ACAGCATGCCAAATCCTTT PRIMER_LEFT_9=6,24 PRIMER_RIGHT_9=2556,19 PRIMER_LEFT_9_TM=64.880 PRIMER_RIGHT_9_TM=58.159 PRIMER_LEFT_9_GC_PERCENT=45.833 PRIMER_RIGHT_9_GC_PERCENT=42.105 PRIMER_LEFT_9_SELF_ANY=8.00 PRIMER_RIGHT_9_SELF_ANY=6.00 PRIMER_LEFT_9_SELF_END=3.00 PRIMER_RIGHT_9_SELF_END=3.00 PRIMER_LEFT_9_POSITION_PENALTY=120.000000 PRIMER_RIGHT_9_POSITION_PENALTY=0.000000 PRIMER_LEFT_9_END_STABILITY=5.2000 PRIMER_RIGHT_9_END_STABILITY=8.5000 PRIMER_PAIR_9_COMPL_ANY=5.00 PRIMER_PAIR_9_COMPL_END=3.00 PRIMER_PRODUCT_SIZE_9=2551 PRIMER_PAIR_PENALTY_10=183.9646 PRIMER_LEFT_10_PENALTY=181.705510 PRIMER_RIGHT_10_PENALTY=2.259083 PRIMER_LEFT_10_SEQUENCE=GCAGCTGCAAAGCATCTAAT PRIMER_RIGHT_10_SEQUENCE=TACAGCATGCCAAATCCTTT PRIMER_LEFT_10=9,20 PRIMER_RIGHT_10=2557,20 PRIMER_LEFT_10_TM=58.294 PRIMER_RIGHT_10_TM=58.241 PRIMER_LEFT_10_GC_PERCENT=45.000 PRIMER_RIGHT_10_GC_PERCENT=40.000 PRIMER_LEFT_10_SELF_ANY=8.00 PRIMER_RIGHT_10_SELF_ANY=6.00 PRIMER_LEFT_10_SELF_END=2.00 PRIMER_RIGHT_10_SELF_END=3.00 PRIMER_LEFT_10_POSITION_PENALTY=180.000000 PRIMER_RIGHT_10_POSITION_PENALTY=0.500000 PRIMER_LEFT_10_END_STABILITY=5.9000 PRIMER_RIGHT_10_END_STABILITY=8.5000 PRIMER_PAIR_10_COMPL_ANY=5.00 PRIMER_PAIR_10_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_10=2549 PRIMER_PAIR_PENALTY_11=184.1054 PRIMER_LEFT_11_PENALTY=181.705510 PRIMER_RIGHT_11_PENALTY=2.399914 PRIMER_LEFT_11_SEQUENCE=GCAGCTGCAAAGCATCTAAT PRIMER_RIGHT_11_SEQUENCE=TTACAGCATGCCAAATCCTTT PRIMER_LEFT_11=9,20 PRIMER_RIGHT_11=2558,21 PRIMER_LEFT_11_TM=58.294 PRIMER_RIGHT_11_TM=59.600 PRIMER_LEFT_11_GC_PERCENT=45.000 PRIMER_RIGHT_11_GC_PERCENT=38.095 PRIMER_LEFT_11_SELF_ANY=8.00 PRIMER_RIGHT_11_SELF_ANY=6.00 PRIMER_LEFT_11_SELF_END=2.00 PRIMER_RIGHT_11_SELF_END=3.00 PRIMER_LEFT_11_POSITION_PENALTY=180.000000 PRIMER_RIGHT_11_POSITION_PENALTY=1.000000 PRIMER_LEFT_11_END_STABILITY=5.9000 PRIMER_RIGHT_11_END_STABILITY=8.5000 PRIMER_PAIR_11_COMPL_ANY=6.00 PRIMER_PAIR_11_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_11=2550 PRIMER_PAIR_PENALTY_12=184.4646 PRIMER_LEFT_12_PENALTY=181.705510 PRIMER_RIGHT_12_PENALTY=2.759083 PRIMER_LEFT_12_SEQUENCE=GCAGCTGCAAAGCATCTAAT PRIMER_RIGHT_12_SEQUENCE=TTACAGCATGCCAAATCCTT PRIMER_LEFT_12=9,20 PRIMER_RIGHT_12=2558,20 PRIMER_LEFT_12_TM=58.294 PRIMER_RIGHT_12_TM=58.241 PRIMER_LEFT_12_GC_PERCENT=45.000 PRIMER_RIGHT_12_GC_PERCENT=40.000 PRIMER_LEFT_12_SELF_ANY=8.00 PRIMER_RIGHT_12_SELF_ANY=6.00 PRIMER_LEFT_12_SELF_END=2.00 PRIMER_RIGHT_12_SELF_END=1.00 PRIMER_LEFT_12_POSITION_PENALTY=180.000000 PRIMER_RIGHT_12_POSITION_PENALTY=1.000000 PRIMER_LEFT_12_END_STABILITY=5.9000 PRIMER_RIGHT_12_END_STABILITY=8.2000 PRIMER_PAIR_12_COMPL_ANY=6.00 PRIMER_PAIR_12_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_12=2550 PRIMER_PAIR_PENALTY_13=184.5465 PRIMER_LEFT_13_PENALTY=181.705510 PRIMER_RIGHT_13_PENALTY=2.840968 PRIMER_LEFT_13_SEQUENCE=GCAGCTGCAAAGCATCTAAT PRIMER_RIGHT_13_SEQUENCE=ACAGCATGCCAAATCCTTT PRIMER_LEFT_13=9,20 PRIMER_RIGHT_13=2556,19 PRIMER_LEFT_13_TM=58.294 PRIMER_RIGHT_13_TM=58.159 PRIMER_LEFT_13_GC_PERCENT=45.000 PRIMER_RIGHT_13_GC_PERCENT=42.105 PRIMER_LEFT_13_SELF_ANY=8.00 PRIMER_RIGHT_13_SELF_ANY=6.00 PRIMER_LEFT_13_SELF_END=2.00 PRIMER_RIGHT_13_SELF_END=3.00 PRIMER_LEFT_13_POSITION_PENALTY=180.000000 PRIMER_RIGHT_13_POSITION_PENALTY=0.000000 PRIMER_LEFT_13_END_STABILITY=5.9000 PRIMER_RIGHT_13_END_STABILITY=8.5000 PRIMER_PAIR_13_COMPL_ANY=5.00 PRIMER_PAIR_13_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_13=2548 PRIMER_PAIR_PENALTY_14=184.8909 PRIMER_LEFT_14_PENALTY=182.631776 PRIMER_RIGHT_14_PENALTY=2.259083 PRIMER_LEFT_14_SEQUENCE=GCAGCTGCAAAGCATCTAATA PRIMER_RIGHT_14_SEQUENCE=TACAGCATGCCAAATCCTTT PRIMER_LEFT_14=9,21 PRIMER_RIGHT_14=2557,20 PRIMER_LEFT_14_TM=58.368 PRIMER_RIGHT_14_TM=58.241 PRIMER_LEFT_14_GC_PERCENT=42.857 PRIMER_RIGHT_14_GC_PERCENT=40.000 PRIMER_LEFT_14_SELF_ANY=8.00 PRIMER_RIGHT_14_SELF_ANY=6.00 PRIMER_LEFT_14_SELF_END=3.00 PRIMER_RIGHT_14_SELF_END=3.00 PRIMER_LEFT_14_POSITION_PENALTY=180.000000 PRIMER_RIGHT_14_POSITION_PENALTY=0.500000 PRIMER_LEFT_14_END_STABILITY=5.2000 PRIMER_RIGHT_14_END_STABILITY=8.5000 PRIMER_PAIR_14_COMPL_ANY=5.00 PRIMER_PAIR_14_COMPL_END=3.00 PRIMER_PRODUCT_SIZE_14=2549 PRIMER_PAIR_PENALTY_15=185.0317 PRIMER_LEFT_15_PENALTY=182.631776 PRIMER_RIGHT_15_PENALTY=2.399914 PRIMER_LEFT_15_SEQUENCE=GCAGCTGCAAAGCATCTAATA PRIMER_RIGHT_15_SEQUENCE=TTACAGCATGCCAAATCCTTT PRIMER_LEFT_15=9,21 PRIMER_RIGHT_15=2558,21 PRIMER_LEFT_15_TM=58.368 PRIMER_RIGHT_15_TM=59.600 PRIMER_LEFT_15_GC_PERCENT=42.857 PRIMER_RIGHT_15_GC_PERCENT=38.095 PRIMER_LEFT_15_SELF_ANY=8.00 PRIMER_RIGHT_15_SELF_ANY=6.00 PRIMER_LEFT_15_SELF_END=3.00 PRIMER_RIGHT_15_SELF_END=3.00 PRIMER_LEFT_15_POSITION_PENALTY=180.000000 PRIMER_RIGHT_15_POSITION_PENALTY=1.000000 PRIMER_LEFT_15_END_STABILITY=5.2000 PRIMER_RIGHT_15_END_STABILITY=8.5000 PRIMER_PAIR_15_COMPL_ANY=6.00 PRIMER_PAIR_15_COMPL_END=3.00 PRIMER_PRODUCT_SIZE_15=2550 PRIMER_PAIR_PENALTY_16=185.3558 PRIMER_LEFT_16_PENALTY=183.096742 PRIMER_RIGHT_16_PENALTY=2.259083 PRIMER_LEFT_16_SEQUENCE=GCAGCTGCAAAGCATCTAA PRIMER_RIGHT_16_SEQUENCE=TACAGCATGCCAAATCCTTT PRIMER_LEFT_16=9,19 PRIMER_RIGHT_16=2557,20 PRIMER_LEFT_16_TM=57.903 PRIMER_RIGHT_16_TM=58.241 PRIMER_LEFT_16_GC_PERCENT=47.368 PRIMER_RIGHT_16_GC_PERCENT=40.000 PRIMER_LEFT_16_SELF_ANY=8.00 PRIMER_RIGHT_16_SELF_ANY=6.00 PRIMER_LEFT_16_SELF_END=1.00 PRIMER_RIGHT_16_SELF_END=3.00 PRIMER_LEFT_16_POSITION_PENALTY=180.000000 PRIMER_RIGHT_16_POSITION_PENALTY=0.500000 PRIMER_LEFT_16_END_STABILITY=6.0000 PRIMER_RIGHT_16_END_STABILITY=8.5000 PRIMER_PAIR_16_COMPL_ANY=5.00 PRIMER_PAIR_16_COMPL_END=3.00 PRIMER_PRODUCT_SIZE_16=2549 PRIMER_PAIR_PENALTY_17=185.3909 PRIMER_LEFT_17_PENALTY=182.631776 PRIMER_RIGHT_17_PENALTY=2.759083 PRIMER_LEFT_17_SEQUENCE=GCAGCTGCAAAGCATCTAATA PRIMER_RIGHT_17_SEQUENCE=TTACAGCATGCCAAATCCTT PRIMER_LEFT_17=9,21 PRIMER_RIGHT_17=2558,20 PRIMER_LEFT_17_TM=58.368 PRIMER_RIGHT_17_TM=58.241 PRIMER_LEFT_17_GC_PERCENT=42.857 PRIMER_RIGHT_17_GC_PERCENT=40.000 PRIMER_LEFT_17_SELF_ANY=8.00 PRIMER_RIGHT_17_SELF_ANY=6.00 PRIMER_LEFT_17_SELF_END=3.00 PRIMER_RIGHT_17_SELF_END=1.00 PRIMER_LEFT_17_POSITION_PENALTY=180.000000 PRIMER_RIGHT_17_POSITION_PENALTY=1.000000 PRIMER_LEFT_17_END_STABILITY=5.2000 PRIMER_RIGHT_17_END_STABILITY=8.2000 PRIMER_PAIR_17_COMPL_ANY=6.00 PRIMER_PAIR_17_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_17=2550 PRIMER_PAIR_PENALTY_18=185.4727 PRIMER_LEFT_18_PENALTY=182.631776 PRIMER_RIGHT_18_PENALTY=2.840968 PRIMER_LEFT_18_SEQUENCE=GCAGCTGCAAAGCATCTAATA PRIMER_RIGHT_18_SEQUENCE=ACAGCATGCCAAATCCTTT PRIMER_LEFT_18=9,21 PRIMER_RIGHT_18=2556,19 PRIMER_LEFT_18_TM=58.368 PRIMER_RIGHT_18_TM=58.159 PRIMER_LEFT_18_GC_PERCENT=42.857 PRIMER_RIGHT_18_GC_PERCENT=42.105 PRIMER_LEFT_18_SELF_ANY=8.00 PRIMER_RIGHT_18_SELF_ANY=6.00 PRIMER_LEFT_18_SELF_END=3.00 PRIMER_RIGHT_18_SELF_END=3.00 PRIMER_LEFT_18_POSITION_PENALTY=180.000000 PRIMER_RIGHT_18_POSITION_PENALTY=0.000000 PRIMER_LEFT_18_END_STABILITY=5.2000 PRIMER_RIGHT_18_END_STABILITY=8.5000 PRIMER_PAIR_18_COMPL_ANY=5.00 PRIMER_PAIR_18_COMPL_END=3.00 PRIMER_PRODUCT_SIZE_18=2548 PRIMER_PAIR_PENALTY_19=185.4967 PRIMER_LEFT_19_PENALTY=183.096742 PRIMER_RIGHT_19_PENALTY=2.399914 PRIMER_LEFT_19_SEQUENCE=GCAGCTGCAAAGCATCTAA PRIMER_RIGHT_19_SEQUENCE=TTACAGCATGCCAAATCCTTT PRIMER_LEFT_19=9,19 PRIMER_RIGHT_19=2558,21 PRIMER_LEFT_19_TM=57.903 PRIMER_RIGHT_19_TM=59.600 PRIMER_LEFT_19_GC_PERCENT=47.368 PRIMER_RIGHT_19_GC_PERCENT=38.095 PRIMER_LEFT_19_SELF_ANY=8.00 PRIMER_RIGHT_19_SELF_ANY=6.00 PRIMER_LEFT_19_SELF_END=1.00 PRIMER_RIGHT_19_SELF_END=3.00 PRIMER_LEFT_19_POSITION_PENALTY=180.000000 PRIMER_RIGHT_19_POSITION_PENALTY=1.000000 PRIMER_LEFT_19_END_STABILITY=6.0000 PRIMER_RIGHT_19_END_STABILITY=8.5000 PRIMER_PAIR_19_COMPL_ANY=6.00 PRIMER_PAIR_19_COMPL_END=3.00 PRIMER_PRODUCT_SIZE_19=2550 = PRIMER_SEQUENCE_ID=L22005/HCDC34 PRIMER_COMMENT=Human ubiquitin conjugating enzyme mRNA, partial cds. CDS=<1..897 PRIMER_START_CODON_POSITION=-3 SEQUENCE=ATTGCGGCGGCGCCAGAGCTGCTGGAGCGCTCGGGGTCCCCGGGCGGCGGCGGCGGCGCAGAGGAGGAGGCAGGCGGCGGCCCCGGTGGCTCCCCCCCGGACGGTGCGCGGCCCGGCCCGTCTCGCGAACTCGCGGTGGTCGCGCGGCCCCGCGCTGCTCCGACCCCGGGCCCCTCCGCCGCCGCCATGGCTCGGCCGCTAGTGCCCAGCTCGCAGAAGGCGCTGCTGCTGGAGCTCAAGGGGCTGCAGGAAGAGCCGGTCGAGGGATTCCGCGTGACACTGGTGGACGAGGGCGATCTATACAACTGGGAGGTGGCCATTTTCGGGCCCCCCAACACCTACTACGAGGGCGGCTACTTCAAGGCGCGCCTCAAGTTCCCCATCGACTACCCATACTCTCCACCAGCCTTTCGGTTCCTGACCAAGATGTGGCACCCTAACATCTACGAGACGGGGGACGTGTGTATCTCCATCCTCCACCCGCCGGTGGACGACCCCCAGAGCGGGGAGCTGCCCTCAGAGAGGTGGAACCCCACGCAGAACGTCAGGACCATTCTCCTGAGTGTGATCTCCCTCCTGAACGAGCCCAACACCTTCTCGCCCGCAAACGTGGACGCCTCCGTGATGTACAGGAAGTGGAAAGAGAGCAAGGGGAAGGATCGGGAGTACACAGACATCATCCGGAAGCAGGTCCTGGGGACCAAGGTGGACGCGGAGCGTGACGGCGTGAAGGTGCCCACCACGCTGGCCGAGTACTGCGTGAAGACCAAGGCGCCGGCGCCCGACGAGGGCTCAGACCTCTTCTACGACGACTACTACGAGGACGGCGAGGTGGAGGAGGAGGCCGACAGCTGCTTCGGGGACGATGAGGATGACTCTGGCACGGAGGAGTCCTGACACCACCAGAATAAACTTGCCGAGTTTACCTCACTAGGGCCGGACCCGTGGCTCCTTAGACGACAGACTACCTCACGGAGGTTTTGTGCTGGTCCCCGTCTCCTCTGGTTGTTTCGTTTTGGCTTTTTCTCCCTCCCCATGTCTGTTCTGGGTTTTCACGTGCTTCAGAGAAGAGGGGCTGCCCCACCGCCACTCACGTCACTCGGGGCTCGGTGGACGGGCCCAGGGTGGGAGCGGCCGGCCCACCTGTCCCCTCGGGAGGGGAGCTGAGCCCGACTTCTACCGGGGTCCCCCAGCTTCCGGACTGGCCGCACCCCGGAGGAGCCACGGGGGCGCTGCTGGGAACGTGGGCGGGGGGCCGTTTCCTGACACTACCAGCCTGGGAGGCCCAGGTGTAGCGGTCCGAGGGGCCCGGTCCTGCCTGTCAGCTCCAGGTCCTGGAGCCACGTCCAGCACTGAGTGGACGGATTCACCAAT PRIMER_LEFT_EXPLAIN=considered 11417, would not amplify any of the ORF 8815, GC content failed 428, low tm 104, high tm 1651, high end compl 2, long poly-x seq 18, high 3' stability 24, ok 375 PRIMER_RIGHT_EXPLAIN=considered 11034, GC content failed 1402, low tm 461, high tm 7364, high end compl 5, long poly-x seq 44, high 3' stability 50, ok 1708 PRIMER_PAIR_EXPLAIN=considered 23, high end compl 2, ok 21 PRIMER_STOP_CODON_POSITION=894 PRIMER_PAIR_PENALTY=4027.9177 PRIMER_LEFT_PENALTY=4023.813185 PRIMER_RIGHT_PENALTY=4.104466 PRIMER_LEFT_SEQUENCE=CTAGTGCCCAGCTCGCAGA PRIMER_RIGHT_SEQUENCE=GGTGTCAGGACTCCTCCGT PRIMER_LEFT=198,19 PRIMER_RIGHT=900,19 PRIMER_LEFT_TM=62.813 PRIMER_RIGHT_TM=60.104 PRIMER_LEFT_GC_PERCENT=63.158 PRIMER_RIGHT_GC_PERCENT=63.158 PRIMER_LEFT_SELF_ANY=4.00 PRIMER_RIGHT_SELF_ANY=7.00 PRIMER_LEFT_SELF_END=2.00 PRIMER_RIGHT_SELF_END=0.00 PRIMER_LEFT_POSITION_PENALTY=4020.000000 PRIMER_RIGHT_POSITION_PENALTY=3.000000 PRIMER_LEFT_END_STABILITY=8.2000 PRIMER_RIGHT_END_STABILITY=9.6000 PRIMER_PAIR_COMPL_ANY=3.00 PRIMER_PAIR_COMPL_END=1.00 PRIMER_PRODUCT_SIZE=703 PRIMER_PAIR_PENALTY_1=4028.1023 PRIMER_LEFT_1_PENALTY=4023.813185 PRIMER_RIGHT_1_PENALTY=4.289096 PRIMER_LEFT_1_SEQUENCE=CTAGTGCCCAGCTCGCAGA PRIMER_RIGHT_1_SEQUENCE=GTGTCAGGACTCCTCCGTG PRIMER_LEFT_1=198,19 PRIMER_RIGHT_1=899,19 PRIMER_LEFT_1_TM=62.813 PRIMER_RIGHT_1_TM=59.211 PRIMER_LEFT_1_GC_PERCENT=63.158 PRIMER_RIGHT_1_GC_PERCENT=63.158 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=7.00 PRIMER_LEFT_1_SELF_END=2.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_LEFT_1_POSITION_PENALTY=4020.000000 PRIMER_RIGHT_1_POSITION_PENALTY=2.500000 PRIMER_LEFT_1_END_STABILITY=8.2000 PRIMER_RIGHT_1_END_STABILITY=9.9000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_1=702 PRIMER_PAIR_PENALTY_2=4028.1071 PRIMER_LEFT_2_PENALTY=4024.002640 PRIMER_RIGHT_2_PENALTY=4.104466 PRIMER_LEFT_2_SEQUENCE=CTAGTGCCCAGCTCGCAGAA PRIMER_RIGHT_2_SEQUENCE=GGTGTCAGGACTCCTCCGT PRIMER_LEFT_2=198,20 PRIMER_RIGHT_2=900,19 PRIMER_LEFT_2_TM=64.003 PRIMER_RIGHT_2_TM=60.104 PRIMER_LEFT_2_GC_PERCENT=60.000 PRIMER_RIGHT_2_GC_PERCENT=63.158 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=7.00 PRIMER_LEFT_2_SELF_END=2.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_LEFT_2_POSITION_PENALTY=4020.000000 PRIMER_RIGHT_2_POSITION_PENALTY=3.000000 PRIMER_LEFT_2_END_STABILITY=7.0000 PRIMER_RIGHT_2_END_STABILITY=9.6000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_2=703 PRIMER_PAIR_PENALTY_3=4028.1792 PRIMER_LEFT_3_PENALTY=4023.813185 PRIMER_RIGHT_3_PENALTY=4.365996 PRIMER_LEFT_3_SEQUENCE=CTAGTGCCCAGCTCGCAGA PRIMER_RIGHT_3_SEQUENCE=AGGACTCCTCCGTGCCAGA PRIMER_LEFT_3=198,19 PRIMER_RIGHT_3=894,19 PRIMER_LEFT_3_TM=62.813 PRIMER_RIGHT_3_TM=63.366 PRIMER_LEFT_3_GC_PERCENT=63.158 PRIMER_RIGHT_3_GC_PERCENT=63.158 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=7.00 PRIMER_LEFT_3_SELF_END=2.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_LEFT_3_POSITION_PENALTY=4020.000000 PRIMER_RIGHT_3_POSITION_PENALTY=0.000000 PRIMER_LEFT_3_END_STABILITY=8.2000 PRIMER_RIGHT_3_END_STABILITY=8.2000 PRIMER_PAIR_3_COMPL_ANY=4.00 PRIMER_PAIR_3_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_3=697 PRIMER_PAIR_PENALTY_4=4028.2917 PRIMER_LEFT_4_PENALTY=4024.002640 PRIMER_RIGHT_4_PENALTY=4.289096 PRIMER_LEFT_4_SEQUENCE=CTAGTGCCCAGCTCGCAGAA PRIMER_RIGHT_4_SEQUENCE=GTGTCAGGACTCCTCCGTG PRIMER_LEFT_4=198,20 PRIMER_RIGHT_4=899,19 PRIMER_LEFT_4_TM=64.003 PRIMER_RIGHT_4_TM=59.211 PRIMER_LEFT_4_GC_PERCENT=60.000 PRIMER_RIGHT_4_GC_PERCENT=63.158 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=7.00 PRIMER_LEFT_4_SELF_END=2.00 PRIMER_RIGHT_4_SELF_END=2.00 PRIMER_LEFT_4_POSITION_PENALTY=4020.000000 PRIMER_RIGHT_4_POSITION_PENALTY=2.500000 PRIMER_LEFT_4_END_STABILITY=7.0000 PRIMER_RIGHT_4_END_STABILITY=9.9000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=3.00 PRIMER_PRODUCT_SIZE_4=702 PRIMER_PAIR_PENALTY_5=4028.3686 PRIMER_LEFT_5_PENALTY=4024.002640 PRIMER_RIGHT_5_PENALTY=4.365996 PRIMER_LEFT_5_SEQUENCE=CTAGTGCCCAGCTCGCAGAA PRIMER_RIGHT_5_SEQUENCE=AGGACTCCTCCGTGCCAGA PRIMER_LEFT_5=198,20 PRIMER_RIGHT_5=894,19 PRIMER_LEFT_5_TM=64.003 PRIMER_RIGHT_5_TM=63.366 PRIMER_LEFT_5_GC_PERCENT=60.000 PRIMER_RIGHT_5_GC_PERCENT=63.158 PRIMER_LEFT_5_SELF_ANY=4.00 PRIMER_RIGHT_5_SELF_ANY=7.00 PRIMER_LEFT_5_SELF_END=2.00 PRIMER_RIGHT_5_SELF_END=2.00 PRIMER_LEFT_5_POSITION_PENALTY=4020.000000 PRIMER_RIGHT_5_POSITION_PENALTY=0.000000 PRIMER_LEFT_5_END_STABILITY=7.0000 PRIMER_RIGHT_5_END_STABILITY=8.2000 PRIMER_PAIR_5_COMPL_ANY=4.00 PRIMER_PAIR_5_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_5=697 PRIMER_PAIR_PENALTY_6=4029.3023 PRIMER_LEFT_6_PENALTY=4023.813185 PRIMER_RIGHT_6_PENALTY=5.489119 PRIMER_LEFT_6_SEQUENCE=CTAGTGCCCAGCTCGCAGA PRIMER_RIGHT_6_SEQUENCE=TGTCAGGACTCCTCCGTGC PRIMER_LEFT_6=198,19 PRIMER_RIGHT_6=898,19 PRIMER_LEFT_6_TM=62.813 PRIMER_RIGHT_6_TM=62.489 PRIMER_LEFT_6_GC_PERCENT=63.158 PRIMER_RIGHT_6_GC_PERCENT=63.158 PRIMER_LEFT_6_SELF_ANY=4.00 PRIMER_RIGHT_6_SELF_ANY=7.00 PRIMER_LEFT_6_SELF_END=2.00 PRIMER_RIGHT_6_SELF_END=2.00 PRIMER_LEFT_6_POSITION_PENALTY=4020.000000 PRIMER_RIGHT_6_POSITION_PENALTY=2.000000 PRIMER_LEFT_6_END_STABILITY=8.2000 PRIMER_RIGHT_6_END_STABILITY=9.9000 PRIMER_PAIR_6_COMPL_ANY=4.00 PRIMER_PAIR_6_COMPL_END=3.00 PRIMER_PRODUCT_SIZE_6=701 PRIMER_PAIR_PENALTY_7=4029.5632 PRIMER_LEFT_7_PENALTY=4023.813185 PRIMER_RIGHT_7_PENALTY=5.750053 PRIMER_LEFT_7_SEQUENCE=CTAGTGCCCAGCTCGCAGA PRIMER_RIGHT_7_SEQUENCE=GTCAGGACTCCTCCGTGCC PRIMER_LEFT_7=198,19 PRIMER_RIGHT_7=897,19 PRIMER_LEFT_7_TM=62.813 PRIMER_RIGHT_7_TM=63.250 PRIMER_LEFT_7_GC_PERCENT=63.158 PRIMER_RIGHT_7_GC_PERCENT=68.421 PRIMER_LEFT_7_SELF_ANY=4.00 PRIMER_RIGHT_7_SELF_ANY=7.00 PRIMER_LEFT_7_SELF_END=2.00 PRIMER_RIGHT_7_SELF_END=2.00 PRIMER_LEFT_7_POSITION_PENALTY=4020.000000 PRIMER_RIGHT_7_POSITION_PENALTY=1.500000 PRIMER_LEFT_7_END_STABILITY=8.2000 PRIMER_RIGHT_7_END_STABILITY=9.4000 PRIMER_PAIR_7_COMPL_ANY=4.00 PRIMER_PAIR_7_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_7=700 PRIMER_PAIR_PENALTY_8=4029.5909 PRIMER_LEFT_8_PENALTY=4023.813185 PRIMER_RIGHT_8_PENALTY=5.777694 PRIMER_LEFT_8_SEQUENCE=CTAGTGCCCAGCTCGCAGA PRIMER_RIGHT_8_SEQUENCE=GTGTCAGGACTCCTCCGTGC PRIMER_LEFT_8=198,19 PRIMER_RIGHT_8=899,20 PRIMER_LEFT_8_TM=62.813 PRIMER_RIGHT_8_TM=63.278 PRIMER_LEFT_8_GC_PERCENT=63.158 PRIMER_RIGHT_8_GC_PERCENT=65.000 PRIMER_LEFT_8_SELF_ANY=4.00 PRIMER_RIGHT_8_SELF_ANY=7.00 PRIMER_LEFT_8_SELF_END=2.00 PRIMER_RIGHT_8_SELF_END=2.00 PRIMER_LEFT_8_POSITION_PENALTY=4020.000000 PRIMER_RIGHT_8_POSITION_PENALTY=2.500000 PRIMER_LEFT_8_END_STABILITY=8.2000 PRIMER_RIGHT_8_END_STABILITY=9.9000 PRIMER_PAIR_8_COMPL_ANY=4.00 PRIMER_PAIR_8_COMPL_END=3.00 PRIMER_PRODUCT_SIZE_8=702 PRIMER_PAIR_PENALTY_9=4029.6579 PRIMER_LEFT_9_PENALTY=4023.813185 PRIMER_RIGHT_9_PENALTY=5.844698 PRIMER_LEFT_9_SEQUENCE=CTAGTGCCCAGCTCGCAGA PRIMER_RIGHT_9_SEQUENCE=AGGACTCCTCCGTGCCAGAGT PRIMER_LEFT_9=198,19 PRIMER_RIGHT_9=894,21 PRIMER_LEFT_9_TM=62.813 PRIMER_RIGHT_9_TM=64.845 PRIMER_LEFT_9_GC_PERCENT=63.158 PRIMER_RIGHT_9_GC_PERCENT=61.905 PRIMER_LEFT_9_SELF_ANY=4.00 PRIMER_RIGHT_9_SELF_ANY=7.00 PRIMER_LEFT_9_SELF_END=2.00 PRIMER_RIGHT_9_SELF_END=2.00 PRIMER_LEFT_9_POSITION_PENALTY=4020.000000 PRIMER_RIGHT_9_POSITION_PENALTY=0.000000 PRIMER_LEFT_9_END_STABILITY=8.2000 PRIMER_RIGHT_9_END_STABILITY=6.1000 PRIMER_PAIR_9_COMPL_ANY=4.00 PRIMER_PAIR_9_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_9=697 PRIMER_PAIR_PENALTY_10=4029.7039 PRIMER_LEFT_10_PENALTY=4023.813185 PRIMER_RIGHT_10_PENALTY=5.890719 PRIMER_LEFT_10_SEQUENCE=CTAGTGCCCAGCTCGCAGA PRIMER_RIGHT_10_SEQUENCE=CAGGACTCCTCCGTGCCAG PRIMER_LEFT_10=198,19 PRIMER_RIGHT_10=895,19 PRIMER_LEFT_10_TM=62.813 PRIMER_RIGHT_10_TM=64.391 PRIMER_LEFT_10_GC_PERCENT=63.158 PRIMER_RIGHT_10_GC_PERCENT=68.421 PRIMER_LEFT_10_SELF_ANY=4.00 PRIMER_RIGHT_10_SELF_ANY=7.00 PRIMER_LEFT_10_SELF_END=2.00 PRIMER_RIGHT_10_SELF_END=1.00 PRIMER_LEFT_10_POSITION_PENALTY=4020.000000 PRIMER_RIGHT_10_POSITION_PENALTY=0.500000 PRIMER_LEFT_10_END_STABILITY=8.2000 PRIMER_RIGHT_10_END_STABILITY=9.7000 PRIMER_PAIR_10_COMPL_ANY=4.00 PRIMER_PAIR_10_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_10=698 PRIMER_PAIR_PENALTY_11=4029.7527 PRIMER_LEFT_11_PENALTY=4024.002640 PRIMER_RIGHT_11_PENALTY=5.750053 PRIMER_LEFT_11_SEQUENCE=CTAGTGCCCAGCTCGCAGAA PRIMER_RIGHT_11_SEQUENCE=GTCAGGACTCCTCCGTGCC PRIMER_LEFT_11=198,20 PRIMER_RIGHT_11=897,19 PRIMER_LEFT_11_TM=64.003 PRIMER_RIGHT_11_TM=63.250 PRIMER_LEFT_11_GC_PERCENT=60.000 PRIMER_RIGHT_11_GC_PERCENT=68.421 PRIMER_LEFT_11_SELF_ANY=4.00 PRIMER_RIGHT_11_SELF_ANY=7.00 PRIMER_LEFT_11_SELF_END=2.00 PRIMER_RIGHT_11_SELF_END=2.00 PRIMER_LEFT_11_POSITION_PENALTY=4020.000000 PRIMER_RIGHT_11_POSITION_PENALTY=1.500000 PRIMER_LEFT_11_END_STABILITY=7.0000 PRIMER_RIGHT_11_END_STABILITY=9.4000 PRIMER_PAIR_11_COMPL_ANY=4.00 PRIMER_PAIR_11_COMPL_END=3.00 PRIMER_PRODUCT_SIZE_11=700 PRIMER_PAIR_PENALTY_12=4029.8473 PRIMER_LEFT_12_PENALTY=4024.002640 PRIMER_RIGHT_12_PENALTY=5.844698 PRIMER_LEFT_12_SEQUENCE=CTAGTGCCCAGCTCGCAGAA PRIMER_RIGHT_12_SEQUENCE=AGGACTCCTCCGTGCCAGAGT PRIMER_LEFT_12=198,20 PRIMER_RIGHT_12=894,21 PRIMER_LEFT_12_TM=64.003 PRIMER_RIGHT_12_TM=64.845 PRIMER_LEFT_12_GC_PERCENT=60.000 PRIMER_RIGHT_12_GC_PERCENT=61.905 PRIMER_LEFT_12_SELF_ANY=4.00 PRIMER_RIGHT_12_SELF_ANY=7.00 PRIMER_LEFT_12_SELF_END=2.00 PRIMER_RIGHT_12_SELF_END=2.00 PRIMER_LEFT_12_POSITION_PENALTY=4020.000000 PRIMER_RIGHT_12_POSITION_PENALTY=0.000000 PRIMER_LEFT_12_END_STABILITY=7.0000 PRIMER_RIGHT_12_END_STABILITY=6.1000 PRIMER_PAIR_12_COMPL_ANY=4.00 PRIMER_PAIR_12_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_12=697 PRIMER_PAIR_PENALTY_13=4029.8934 PRIMER_LEFT_13_PENALTY=4024.002640 PRIMER_RIGHT_13_PENALTY=5.890719 PRIMER_LEFT_13_SEQUENCE=CTAGTGCCCAGCTCGCAGAA PRIMER_RIGHT_13_SEQUENCE=CAGGACTCCTCCGTGCCAG PRIMER_LEFT_13=198,20 PRIMER_RIGHT_13=895,19 PRIMER_LEFT_13_TM=64.003 PRIMER_RIGHT_13_TM=64.391 PRIMER_LEFT_13_GC_PERCENT=60.000 PRIMER_RIGHT_13_GC_PERCENT=68.421 PRIMER_LEFT_13_SELF_ANY=4.00 PRIMER_RIGHT_13_SELF_ANY=7.00 PRIMER_LEFT_13_SELF_END=2.00 PRIMER_RIGHT_13_SELF_END=1.00 PRIMER_LEFT_13_POSITION_PENALTY=4020.000000 PRIMER_RIGHT_13_POSITION_PENALTY=0.500000 PRIMER_LEFT_13_END_STABILITY=7.0000 PRIMER_RIGHT_13_END_STABILITY=9.7000 PRIMER_PAIR_13_COMPL_ANY=4.00 PRIMER_PAIR_13_COMPL_END=3.00 PRIMER_PRODUCT_SIZE_13=698 PRIMER_PAIR_PENALTY_14=4029.9325 PRIMER_LEFT_14_PENALTY=4023.813185 PRIMER_RIGHT_14_PENALTY=6.119361 PRIMER_LEFT_14_SEQUENCE=CTAGTGCCCAGCTCGCAGA PRIMER_RIGHT_14_SEQUENCE=GGTGTCAGGACTCCTCCGTG PRIMER_LEFT_14=198,19 PRIMER_RIGHT_14=900,20 PRIMER_LEFT_14_TM=62.813 PRIMER_RIGHT_14_TM=63.119 PRIMER_LEFT_14_GC_PERCENT=63.158 PRIMER_RIGHT_14_GC_PERCENT=65.000 PRIMER_LEFT_14_SELF_ANY=4.00 PRIMER_RIGHT_14_SELF_ANY=7.00 PRIMER_LEFT_14_SELF_END=2.00 PRIMER_RIGHT_14_SELF_END=2.00 PRIMER_LEFT_14_POSITION_PENALTY=4020.000000 PRIMER_RIGHT_14_POSITION_PENALTY=3.000000 PRIMER_LEFT_14_END_STABILITY=8.2000 PRIMER_RIGHT_14_END_STABILITY=9.9000 PRIMER_PAIR_14_COMPL_ANY=3.00 PRIMER_PAIR_14_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_14=703 PRIMER_PAIR_PENALTY_15=4030.1220 PRIMER_LEFT_15_PENALTY=4024.002640 PRIMER_RIGHT_15_PENALTY=6.119361 PRIMER_LEFT_15_SEQUENCE=CTAGTGCCCAGCTCGCAGAA PRIMER_RIGHT_15_SEQUENCE=GGTGTCAGGACTCCTCCGTG PRIMER_LEFT_15=198,20 PRIMER_RIGHT_15=900,20 PRIMER_LEFT_15_TM=64.003 PRIMER_RIGHT_15_TM=63.119 PRIMER_LEFT_15_GC_PERCENT=60.000 PRIMER_RIGHT_15_GC_PERCENT=65.000 PRIMER_LEFT_15_SELF_ANY=4.00 PRIMER_RIGHT_15_SELF_ANY=7.00 PRIMER_LEFT_15_SELF_END=2.00 PRIMER_RIGHT_15_SELF_END=2.00 PRIMER_LEFT_15_POSITION_PENALTY=4020.000000 PRIMER_RIGHT_15_POSITION_PENALTY=3.000000 PRIMER_LEFT_15_END_STABILITY=7.0000 PRIMER_RIGHT_15_END_STABILITY=9.9000 PRIMER_PAIR_15_COMPL_ANY=3.00 PRIMER_PAIR_15_COMPL_END=3.00 PRIMER_PRODUCT_SIZE_15=703 PRIMER_PAIR_PENALTY_16=4030.4325 PRIMER_LEFT_16_PENALTY=4023.813185 PRIMER_RIGHT_16_PENALTY=6.619361 PRIMER_LEFT_16_SEQUENCE=CTAGTGCCCAGCTCGCAGA PRIMER_RIGHT_16_SEQUENCE=TGGTGTCAGGACTCCTCCGT PRIMER_LEFT_16=198,19 PRIMER_RIGHT_16=901,20 PRIMER_LEFT_16_TM=62.813 PRIMER_RIGHT_16_TM=63.119 PRIMER_LEFT_16_GC_PERCENT=63.158 PRIMER_RIGHT_16_GC_PERCENT=60.000 PRIMER_LEFT_16_SELF_ANY=4.00 PRIMER_RIGHT_16_SELF_ANY=7.00 PRIMER_LEFT_16_SELF_END=2.00 PRIMER_RIGHT_16_SELF_END=0.00 PRIMER_LEFT_16_POSITION_PENALTY=4020.000000 PRIMER_RIGHT_16_POSITION_PENALTY=3.500000 PRIMER_LEFT_16_END_STABILITY=8.2000 PRIMER_RIGHT_16_END_STABILITY=9.6000 PRIMER_PAIR_16_COMPL_ANY=3.00 PRIMER_PAIR_16_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_16=704 PRIMER_PAIR_PENALTY_17=4030.6220 PRIMER_LEFT_17_PENALTY=4024.002640 PRIMER_RIGHT_17_PENALTY=6.619361 PRIMER_LEFT_17_SEQUENCE=CTAGTGCCCAGCTCGCAGAA PRIMER_RIGHT_17_SEQUENCE=TGGTGTCAGGACTCCTCCGT PRIMER_LEFT_17=198,20 PRIMER_RIGHT_17=901,20 PRIMER_LEFT_17_TM=64.003 PRIMER_RIGHT_17_TM=63.119 PRIMER_LEFT_17_GC_PERCENT=60.000 PRIMER_RIGHT_17_GC_PERCENT=60.000 PRIMER_LEFT_17_SELF_ANY=4.00 PRIMER_RIGHT_17_SELF_ANY=7.00 PRIMER_LEFT_17_SELF_END=2.00 PRIMER_RIGHT_17_SELF_END=0.00 PRIMER_LEFT_17_POSITION_PENALTY=4020.000000 PRIMER_RIGHT_17_POSITION_PENALTY=3.500000 PRIMER_LEFT_17_END_STABILITY=7.0000 PRIMER_RIGHT_17_END_STABILITY=9.6000 PRIMER_PAIR_17_COMPL_ANY=3.00 PRIMER_PAIR_17_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_17=704 PRIMER_PAIR_PENALTY_18=4030.6367 PRIMER_LEFT_18_PENALTY=4023.813185 PRIMER_RIGHT_18_PENALTY=6.823526 PRIMER_LEFT_18_SEQUENCE=CTAGTGCCCAGCTCGCAGA PRIMER_RIGHT_18_SEQUENCE=TGGTGTCAGGACTCCTCCG PRIMER_LEFT_18=198,19 PRIMER_RIGHT_18=901,19 PRIMER_LEFT_18_TM=62.813 PRIMER_RIGHT_18_TM=62.324 PRIMER_LEFT_18_GC_PERCENT=63.158 PRIMER_RIGHT_18_GC_PERCENT=63.158 PRIMER_LEFT_18_SELF_ANY=4.00 PRIMER_RIGHT_18_SELF_ANY=7.00 PRIMER_LEFT_18_SELF_END=2.00 PRIMER_RIGHT_18_SELF_END=2.00 PRIMER_LEFT_18_POSITION_PENALTY=4020.000000 PRIMER_RIGHT_18_POSITION_PENALTY=3.500000 PRIMER_LEFT_18_END_STABILITY=8.2000 PRIMER_RIGHT_18_END_STABILITY=9.9000 PRIMER_PAIR_18_COMPL_ANY=3.00 PRIMER_PAIR_18_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_18=704 PRIMER_PAIR_PENALTY_19=4030.8262 PRIMER_LEFT_19_PENALTY=4024.002640 PRIMER_RIGHT_19_PENALTY=6.823526 PRIMER_LEFT_19_SEQUENCE=CTAGTGCCCAGCTCGCAGAA PRIMER_RIGHT_19_SEQUENCE=TGGTGTCAGGACTCCTCCG PRIMER_LEFT_19=198,20 PRIMER_RIGHT_19=901,19 PRIMER_LEFT_19_TM=64.003 PRIMER_RIGHT_19_TM=62.324 PRIMER_LEFT_19_GC_PERCENT=60.000 PRIMER_RIGHT_19_GC_PERCENT=63.158 PRIMER_LEFT_19_SELF_ANY=4.00 PRIMER_RIGHT_19_SELF_ANY=7.00 PRIMER_LEFT_19_SELF_END=2.00 PRIMER_RIGHT_19_SELF_END=2.00 PRIMER_LEFT_19_POSITION_PENALTY=4020.000000 PRIMER_RIGHT_19_POSITION_PENALTY=3.500000 PRIMER_LEFT_19_END_STABILITY=7.0000 PRIMER_RIGHT_19_END_STABILITY=9.9000 PRIMER_PAIR_19_COMPL_ANY=3.00 PRIMER_PAIR_19_COMPL_END=3.00 PRIMER_PRODUCT_SIZE_19=704 = PRIMER_SEQUENCE_ID=M24194/MHC/RACK1 PRIMER_COMMENT=Human MHC protein homologous to chicken B complex protein mRNA, complete cds. CDS=96..1049 PRIMER_START_CODON_POSITION=95 SEQUENCE=CTGCAAGGCGGCGGCAGGAGAGGTTGTGGTGCTAGTTTCTCTAAGCCATCCAGTGCCATCCTCGTCGCTGCAGCGACACCGCTCTCGCCGCCGCCATGACTGAGCAGATGACCCTTCGTGGCACCCTCAAGGGCCACAACGGCTGGGTAACCCAGATCGCTACTACCCCGCAGTTCCCGGACATGATCCTCTCCGCCTCTCGAGATAAGACCATCATCATGTGGAAACTGACCAGGGATGAGACCAACTATGGAATTCCACAGCGTGCTCTGCGGGGTCACTCCCACTTTGTTAGTGATGTGGTTATCTCCTCAGATGGCCAGTTTGCCCTCTCAGGCTCCTGGGATGGAACCCTGCGCCTCTGGGATCTCACAACGGGCACCACCACGAGGCGATTTGTGGGCCATACCAAGGATGTGCTGAGTGTGGCCTTCTCCTCTGACAACCGGCAGATTGTCTCTGGATCTCGAGATAAAACCATCAAGCTATGGAATACCCTGGGTGTGTGCAAATACACTGTCCAGGATGAGAGCCACTCAGAGTGGGTGTCTTGTGTCCGCTTCTCGCCCAACAGCAGCAACCCTATCATCGTCTCCTGTGGCTGGGACAAGCTGGTCAAGGTATGGAACCTGGCTAACTGCAAGCTGAAGACCAACCACATTGGCCACACAGGCTATCTGAACACGGTGACTGTCTCTCCAGATGGATCCCTCTGTGCTTCTGGAGGCAAGGATGGCCAGGCCATGTTATGGGATCTCAACGAAGGCAAACACCTTTACACGCTAGATGGTGGGGACATCATCAACGCCCTGTGCTTCAGCCCTAACCGCTACTGGCTGTGTGCTGCCACAGGCCCCAGCATCAAGATCTGGGATTTAGAGGGAAAGATCATTGTAGATGAACTGAAGCAAGAAGTTATCAGTACCAGCAGCAAGGCAGAACCACCCCAGTGCACTTCCCTGGCCTGGTCTGCTGATGGCCAGACTCTGTTTGCTGGCTACACGGACAACCTGGTGCGAGTGTGGCAGGTGACCATTGGCACACGCTAGAAGTTTATGGCAGAGCTTTACAAATAAAAAAAAAATGGCTTTTC PRIMER_LEFT_EXPLAIN=considered 8997, would not amplify any of the ORF 6119, GC content failed 1, low tm 301, high tm 1735, high 3' stability 24, ok 817 PRIMER_RIGHT_EXPLAIN=considered 8758, GC content failed 64, low tm 957, high tm 5153, high end compl 7, long poly-x seq 16, high 3' stability 45, ok 2516 PRIMER_PAIR_EXPLAIN=considered 38, high end compl 14, ok 24 PRIMER_STOP_CODON_POSITION=1046 PRIMER_PAIR_PENALTY=5.4381 PRIMER_LEFT_PENALTY=2.658657 PRIMER_RIGHT_PENALTY=2.779412 PRIMER_LEFT_SEQUENCE=ATGACTGAGCAGATGACCCTTC PRIMER_RIGHT_SEQUENCE=TCTAGCGTGTGCCAATGGT PRIMER_LEFT=95,22 PRIMER_RIGHT=1049,19 PRIMER_LEFT_TM=60.659 PRIMER_RIGHT_TM=60.279 PRIMER_LEFT_GC_PERCENT=50.000 PRIMER_RIGHT_GC_PERCENT=52.632 PRIMER_LEFT_SELF_ANY=4.00 PRIMER_RIGHT_SELF_ANY=5.00 PRIMER_LEFT_SELF_END=1.00 PRIMER_RIGHT_SELF_END=3.00 PRIMER_LEFT_POSITION_PENALTY=0.000000 PRIMER_RIGHT_POSITION_PENALTY=1.500000 PRIMER_LEFT_END_STABILITY=8.2000 PRIMER_RIGHT_END_STABILITY=7.8000 PRIMER_PAIR_COMPL_ANY=4.00 PRIMER_PAIR_COMPL_END=3.00 PRIMER_PRODUCT_SIZE=955 PRIMER_PAIR_PENALTY_1=5.5682 PRIMER_LEFT_1_PENALTY=2.788786 PRIMER_RIGHT_1_PENALTY=2.779412 PRIMER_LEFT_1_SEQUENCE=ATGACTGAGCAGATGACCCT PRIMER_RIGHT_1_SEQUENCE=TCTAGCGTGTGCCAATGGT PRIMER_LEFT_1=95,20 PRIMER_RIGHT_1=1049,19 PRIMER_LEFT_1_TM=57.211 PRIMER_RIGHT_1_TM=60.279 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=52.632 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=5.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_LEFT_1_POSITION_PENALTY=0.000000 PRIMER_RIGHT_1_POSITION_PENALTY=1.500000 PRIMER_LEFT_1_END_STABILITY=9.1000 PRIMER_RIGHT_1_END_STABILITY=7.8000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=3.00 PRIMER_PRODUCT_SIZE_1=955 PRIMER_PAIR_PENALTY_2=6.2893 PRIMER_LEFT_2_PENALTY=2.658657 PRIMER_RIGHT_2_PENALTY=3.630671 PRIMER_LEFT_2_SEQUENCE=ATGACTGAGCAGATGACCCTTC PRIMER_RIGHT_2_SEQUENCE=TTCTAGCGTGTGCCAATGGT PRIMER_LEFT_2=95,22 PRIMER_RIGHT_2=1050,20 PRIMER_LEFT_2_TM=60.659 PRIMER_RIGHT_2_TM=61.631 PRIMER_LEFT_2_GC_PERCENT=50.000 PRIMER_RIGHT_2_GC_PERCENT=50.000 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=5.00 PRIMER_LEFT_2_SELF_END=1.00 PRIMER_RIGHT_2_SELF_END=3.00 PRIMER_LEFT_2_POSITION_PENALTY=0.000000 PRIMER_RIGHT_2_POSITION_PENALTY=2.000000 PRIMER_LEFT_2_END_STABILITY=8.2000 PRIMER_RIGHT_2_END_STABILITY=7.8000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=3.00 PRIMER_PRODUCT_SIZE_2=956 PRIMER_PAIR_PENALTY_3=6.2897 PRIMER_LEFT_3_PENALTY=2.241606 PRIMER_RIGHT_3_PENALTY=4.048100 PRIMER_LEFT_3_SEQUENCE=ATGACTGAGCAGATGACCCTT PRIMER_RIGHT_3_SEQUENCE=ACTTCTAGCGTGTGCCAATG PRIMER_LEFT_3=95,21 PRIMER_RIGHT_3=1052,20 PRIMER_LEFT_3_TM=58.758 PRIMER_RIGHT_3_TM=58.952 PRIMER_LEFT_3_GC_PERCENT=47.619 PRIMER_RIGHT_3_GC_PERCENT=50.000 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=3.00 PRIMER_LEFT_3_POSITION_PENALTY=0.000000 PRIMER_RIGHT_3_POSITION_PENALTY=3.000000 PRIMER_LEFT_3_END_STABILITY=9.7000 PRIMER_RIGHT_3_END_STABILITY=7.2000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_3=958 PRIMER_PAIR_PENALTY_4=6.4195 PRIMER_LEFT_4_PENALTY=2.788786 PRIMER_RIGHT_4_PENALTY=3.630671 PRIMER_LEFT_4_SEQUENCE=ATGACTGAGCAGATGACCCT PRIMER_RIGHT_4_SEQUENCE=TTCTAGCGTGTGCCAATGGT PRIMER_LEFT_4=95,20 PRIMER_RIGHT_4=1050,20 PRIMER_LEFT_4_TM=57.211 PRIMER_RIGHT_4_TM=61.631 PRIMER_LEFT_4_GC_PERCENT=50.000 PRIMER_RIGHT_4_GC_PERCENT=50.000 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=5.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=3.00 PRIMER_LEFT_4_POSITION_PENALTY=0.000000 PRIMER_RIGHT_4_POSITION_PENALTY=2.000000 PRIMER_LEFT_4_END_STABILITY=9.1000 PRIMER_RIGHT_4_END_STABILITY=7.8000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=3.00 PRIMER_PRODUCT_SIZE_4=956 PRIMER_PAIR_PENALTY_5=6.7068 PRIMER_LEFT_5_PENALTY=2.658657 PRIMER_RIGHT_5_PENALTY=4.048100 PRIMER_LEFT_5_SEQUENCE=ATGACTGAGCAGATGACCCTTC PRIMER_RIGHT_5_SEQUENCE=ACTTCTAGCGTGTGCCAATG PRIMER_LEFT_5=95,22 PRIMER_RIGHT_5=1052,20 PRIMER_LEFT_5_TM=60.659 PRIMER_RIGHT_5_TM=58.952 PRIMER_LEFT_5_GC_PERCENT=50.000 PRIMER_RIGHT_5_GC_PERCENT=50.000 PRIMER_LEFT_5_SELF_ANY=4.00 PRIMER_RIGHT_5_SELF_ANY=4.00 PRIMER_LEFT_5_SELF_END=1.00 PRIMER_RIGHT_5_SELF_END=3.00 PRIMER_LEFT_5_POSITION_PENALTY=0.000000 PRIMER_RIGHT_5_POSITION_PENALTY=3.000000 PRIMER_LEFT_5_END_STABILITY=8.2000 PRIMER_RIGHT_5_END_STABILITY=7.2000 PRIMER_PAIR_5_COMPL_ANY=3.00 PRIMER_PAIR_5_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_5=958 PRIMER_PAIR_PENALTY_6=6.8369 PRIMER_LEFT_6_PENALTY=2.788786 PRIMER_RIGHT_6_PENALTY=4.048100 PRIMER_LEFT_6_SEQUENCE=ATGACTGAGCAGATGACCCT PRIMER_RIGHT_6_SEQUENCE=ACTTCTAGCGTGTGCCAATG PRIMER_LEFT_6=95,20 PRIMER_RIGHT_6=1052,20 PRIMER_LEFT_6_TM=57.211 PRIMER_RIGHT_6_TM=58.952 PRIMER_LEFT_6_GC_PERCENT=50.000 PRIMER_RIGHT_6_GC_PERCENT=50.000 PRIMER_LEFT_6_SELF_ANY=4.00 PRIMER_RIGHT_6_SELF_ANY=4.00 PRIMER_LEFT_6_SELF_END=0.00 PRIMER_RIGHT_6_SELF_END=3.00 PRIMER_LEFT_6_POSITION_PENALTY=0.000000 PRIMER_RIGHT_6_POSITION_PENALTY=3.000000 PRIMER_LEFT_6_END_STABILITY=9.1000 PRIMER_RIGHT_6_END_STABILITY=7.2000 PRIMER_PAIR_6_COMPL_ANY=3.00 PRIMER_PAIR_6_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_6=958 PRIMER_PAIR_PENALTY_7=7.0610 PRIMER_LEFT_7_PENALTY=2.241606 PRIMER_RIGHT_7_PENALTY=4.819419 PRIMER_LEFT_7_SEQUENCE=ATGACTGAGCAGATGACCCTT PRIMER_RIGHT_7_SEQUENCE=AACTTCTAGCGTGTGCCAATG PRIMER_LEFT_7=95,21 PRIMER_RIGHT_7=1053,21 PRIMER_LEFT_7_TM=58.758 PRIMER_RIGHT_7_TM=60.319 PRIMER_LEFT_7_GC_PERCENT=47.619 PRIMER_RIGHT_7_GC_PERCENT=47.619 PRIMER_LEFT_7_SELF_ANY=4.00 PRIMER_RIGHT_7_SELF_ANY=4.00 PRIMER_LEFT_7_SELF_END=0.00 PRIMER_RIGHT_7_SELF_END=3.00 PRIMER_LEFT_7_POSITION_PENALTY=0.000000 PRIMER_RIGHT_7_POSITION_PENALTY=3.500000 PRIMER_LEFT_7_END_STABILITY=9.7000 PRIMER_RIGHT_7_END_STABILITY=7.2000 PRIMER_PAIR_7_COMPL_ANY=3.00 PRIMER_PAIR_7_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_7=959 PRIMER_PAIR_PENALTY_8=7.4781 PRIMER_LEFT_8_PENALTY=2.658657 PRIMER_RIGHT_8_PENALTY=4.819419 PRIMER_LEFT_8_SEQUENCE=ATGACTGAGCAGATGACCCTTC PRIMER_RIGHT_8_SEQUENCE=AACTTCTAGCGTGTGCCAATG PRIMER_LEFT_8=95,22 PRIMER_RIGHT_8=1053,21 PRIMER_LEFT_8_TM=60.659 PRIMER_RIGHT_8_TM=60.319 PRIMER_LEFT_8_GC_PERCENT=50.000 PRIMER_RIGHT_8_GC_PERCENT=47.619 PRIMER_LEFT_8_SELF_ANY=4.00 PRIMER_RIGHT_8_SELF_ANY=4.00 PRIMER_LEFT_8_SELF_END=1.00 PRIMER_RIGHT_8_SELF_END=3.00 PRIMER_LEFT_8_POSITION_PENALTY=0.000000 PRIMER_RIGHT_8_POSITION_PENALTY=3.500000 PRIMER_LEFT_8_END_STABILITY=8.2000 PRIMER_RIGHT_8_END_STABILITY=7.2000 PRIMER_PAIR_8_COMPL_ANY=3.00 PRIMER_PAIR_8_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_8=959 PRIMER_PAIR_PENALTY_9=7.6082 PRIMER_LEFT_9_PENALTY=2.788786 PRIMER_RIGHT_9_PENALTY=4.819419 PRIMER_LEFT_9_SEQUENCE=ATGACTGAGCAGATGACCCT PRIMER_RIGHT_9_SEQUENCE=AACTTCTAGCGTGTGCCAATG PRIMER_LEFT_9=95,20 PRIMER_RIGHT_9=1053,21 PRIMER_LEFT_9_TM=57.211 PRIMER_RIGHT_9_TM=60.319 PRIMER_LEFT_9_GC_PERCENT=50.000 PRIMER_RIGHT_9_GC_PERCENT=47.619 PRIMER_LEFT_9_SELF_ANY=4.00 PRIMER_RIGHT_9_SELF_ANY=4.00 PRIMER_LEFT_9_SELF_END=0.00 PRIMER_RIGHT_9_SELF_END=3.00 PRIMER_LEFT_9_POSITION_PENALTY=0.000000 PRIMER_RIGHT_9_POSITION_PENALTY=3.500000 PRIMER_LEFT_9_END_STABILITY=9.1000 PRIMER_RIGHT_9_END_STABILITY=7.2000 PRIMER_PAIR_9_COMPL_ANY=3.00 PRIMER_PAIR_9_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_9=959 PRIMER_PAIR_PENALTY_10=7.6615 PRIMER_LEFT_10_PENALTY=2.241606 PRIMER_RIGHT_10_PENALTY=5.419920 PRIMER_LEFT_10_SEQUENCE=ATGACTGAGCAGATGACCCTT PRIMER_RIGHT_10_SEQUENCE=AAACTTCTAGCGTGTGCCAA PRIMER_LEFT_10=95,21 PRIMER_RIGHT_10=1054,20 PRIMER_LEFT_10_TM=58.758 PRIMER_RIGHT_10_TM=58.580 PRIMER_LEFT_10_GC_PERCENT=47.619 PRIMER_RIGHT_10_GC_PERCENT=45.000 PRIMER_LEFT_10_SELF_ANY=4.00 PRIMER_RIGHT_10_SELF_ANY=4.00 PRIMER_LEFT_10_SELF_END=0.00 PRIMER_RIGHT_10_SELF_END=1.00 PRIMER_LEFT_10_POSITION_PENALTY=0.000000 PRIMER_RIGHT_10_POSITION_PENALTY=4.000000 PRIMER_LEFT_10_END_STABILITY=9.7000 PRIMER_RIGHT_10_END_STABILITY=10.0000 PRIMER_PAIR_10_COMPL_ANY=3.00 PRIMER_PAIR_10_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_10=960 PRIMER_PAIR_PENALTY_11=7.7289 PRIMER_LEFT_11_PENALTY=2.241606 PRIMER_RIGHT_11_PENALTY=5.487331 PRIMER_LEFT_11_SEQUENCE=ATGACTGAGCAGATGACCCTT PRIMER_RIGHT_11_SEQUENCE=CTTCTAGCGTGTGCCAATG PRIMER_LEFT_11=95,21 PRIMER_RIGHT_11=1051,19 PRIMER_LEFT_11_TM=58.758 PRIMER_RIGHT_11_TM=58.013 PRIMER_LEFT_11_GC_PERCENT=47.619 PRIMER_RIGHT_11_GC_PERCENT=52.632 PRIMER_LEFT_11_SELF_ANY=4.00 PRIMER_RIGHT_11_SELF_ANY=4.00 PRIMER_LEFT_11_SELF_END=0.00 PRIMER_RIGHT_11_SELF_END=3.00 PRIMER_LEFT_11_POSITION_PENALTY=0.000000 PRIMER_RIGHT_11_POSITION_PENALTY=2.500000 PRIMER_LEFT_11_END_STABILITY=9.7000 PRIMER_RIGHT_11_END_STABILITY=7.2000 PRIMER_PAIR_11_COMPL_ANY=3.00 PRIMER_PAIR_11_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_11=957 PRIMER_PAIR_PENALTY_12=8.0786 PRIMER_LEFT_12_PENALTY=2.658657 PRIMER_RIGHT_12_PENALTY=5.419920 PRIMER_LEFT_12_SEQUENCE=ATGACTGAGCAGATGACCCTTC PRIMER_RIGHT_12_SEQUENCE=AAACTTCTAGCGTGTGCCAA PRIMER_LEFT_12=95,22 PRIMER_RIGHT_12=1054,20 PRIMER_LEFT_12_TM=60.659 PRIMER_RIGHT_12_TM=58.580 PRIMER_LEFT_12_GC_PERCENT=50.000 PRIMER_RIGHT_12_GC_PERCENT=45.000 PRIMER_LEFT_12_SELF_ANY=4.00 PRIMER_RIGHT_12_SELF_ANY=4.00 PRIMER_LEFT_12_SELF_END=1.00 PRIMER_RIGHT_12_SELF_END=1.00 PRIMER_LEFT_12_POSITION_PENALTY=0.000000 PRIMER_RIGHT_12_POSITION_PENALTY=4.000000 PRIMER_LEFT_12_END_STABILITY=8.2000 PRIMER_RIGHT_12_END_STABILITY=10.0000 PRIMER_PAIR_12_COMPL_ANY=3.00 PRIMER_PAIR_12_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_12=960 PRIMER_PAIR_PENALTY_13=8.1460 PRIMER_LEFT_13_PENALTY=2.658657 PRIMER_RIGHT_13_PENALTY=5.487331 PRIMER_LEFT_13_SEQUENCE=ATGACTGAGCAGATGACCCTTC PRIMER_RIGHT_13_SEQUENCE=CTTCTAGCGTGTGCCAATG PRIMER_LEFT_13=95,22 PRIMER_RIGHT_13=1051,19 PRIMER_LEFT_13_TM=60.659 PRIMER_RIGHT_13_TM=58.013 PRIMER_LEFT_13_GC_PERCENT=50.000 PRIMER_RIGHT_13_GC_PERCENT=52.632 PRIMER_LEFT_13_SELF_ANY=4.00 PRIMER_RIGHT_13_SELF_ANY=4.00 PRIMER_LEFT_13_SELF_END=1.00 PRIMER_RIGHT_13_SELF_END=3.00 PRIMER_LEFT_13_POSITION_PENALTY=0.000000 PRIMER_RIGHT_13_POSITION_PENALTY=2.500000 PRIMER_LEFT_13_END_STABILITY=8.2000 PRIMER_RIGHT_13_END_STABILITY=7.2000 PRIMER_PAIR_13_COMPL_ANY=3.00 PRIMER_PAIR_13_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_13=957 PRIMER_PAIR_PENALTY_14=8.2087 PRIMER_LEFT_14_PENALTY=2.788786 PRIMER_RIGHT_14_PENALTY=5.419920 PRIMER_LEFT_14_SEQUENCE=ATGACTGAGCAGATGACCCT PRIMER_RIGHT_14_SEQUENCE=AAACTTCTAGCGTGTGCCAA PRIMER_LEFT_14=95,20 PRIMER_RIGHT_14=1054,20 PRIMER_LEFT_14_TM=57.211 PRIMER_RIGHT_14_TM=58.580 PRIMER_LEFT_14_GC_PERCENT=50.000 PRIMER_RIGHT_14_GC_PERCENT=45.000 PRIMER_LEFT_14_SELF_ANY=4.00 PRIMER_RIGHT_14_SELF_ANY=4.00 PRIMER_LEFT_14_SELF_END=0.00 PRIMER_RIGHT_14_SELF_END=1.00 PRIMER_LEFT_14_POSITION_PENALTY=0.000000 PRIMER_RIGHT_14_POSITION_PENALTY=4.000000 PRIMER_LEFT_14_END_STABILITY=9.1000 PRIMER_RIGHT_14_END_STABILITY=10.0000 PRIMER_PAIR_14_COMPL_ANY=3.00 PRIMER_PAIR_14_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_14=960 PRIMER_PAIR_PENALTY_15=8.2320 PRIMER_LEFT_15_PENALTY=2.241606 PRIMER_RIGHT_15_PENALTY=5.990437 PRIMER_LEFT_15_SEQUENCE=ATGACTGAGCAGATGACCCTT PRIMER_RIGHT_15_SEQUENCE=AACTTCTAGCGTGTGCCAAT PRIMER_LEFT_15=95,21 PRIMER_RIGHT_15=1053,20 PRIMER_LEFT_15_TM=58.758 PRIMER_RIGHT_15_TM=57.510 PRIMER_LEFT_15_GC_PERCENT=47.619 PRIMER_RIGHT_15_GC_PERCENT=45.000 PRIMER_LEFT_15_SELF_ANY=4.00 PRIMER_RIGHT_15_SELF_ANY=4.00 PRIMER_LEFT_15_SELF_END=0.00 PRIMER_RIGHT_15_SELF_END=2.00 PRIMER_LEFT_15_POSITION_PENALTY=0.000000 PRIMER_RIGHT_15_POSITION_PENALTY=3.500000 PRIMER_LEFT_15_END_STABILITY=9.7000 PRIMER_RIGHT_15_END_STABILITY=8.4000 PRIMER_PAIR_15_COMPL_ANY=3.00 PRIMER_PAIR_15_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_15=959 PRIMER_PAIR_PENALTY_16=8.2761 PRIMER_LEFT_16_PENALTY=2.788786 PRIMER_RIGHT_16_PENALTY=5.487331 PRIMER_LEFT_16_SEQUENCE=ATGACTGAGCAGATGACCCT PRIMER_RIGHT_16_SEQUENCE=CTTCTAGCGTGTGCCAATG PRIMER_LEFT_16=95,20 PRIMER_RIGHT_16=1051,19 PRIMER_LEFT_16_TM=57.211 PRIMER_RIGHT_16_TM=58.013 PRIMER_LEFT_16_GC_PERCENT=50.000 PRIMER_RIGHT_16_GC_PERCENT=52.632 PRIMER_LEFT_16_SELF_ANY=4.00 PRIMER_RIGHT_16_SELF_ANY=4.00 PRIMER_LEFT_16_SELF_END=0.00 PRIMER_RIGHT_16_SELF_END=3.00 PRIMER_LEFT_16_POSITION_PENALTY=0.000000 PRIMER_RIGHT_16_POSITION_PENALTY=2.500000 PRIMER_LEFT_16_END_STABILITY=9.1000 PRIMER_RIGHT_16_END_STABILITY=7.2000 PRIMER_PAIR_16_COMPL_ANY=3.00 PRIMER_PAIR_16_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_16=957 PRIMER_PAIR_PENALTY_17=8.3221 PRIMER_LEFT_17_PENALTY=2.241606 PRIMER_RIGHT_17_PENALTY=6.080486 PRIMER_LEFT_17_SEQUENCE=ATGACTGAGCAGATGACCCTT PRIMER_RIGHT_17_SEQUENCE=AAACTTCTAGCGTGTGCCAAT PRIMER_LEFT_17=95,21 PRIMER_RIGHT_17=1054,21 PRIMER_LEFT_17_TM=58.758 PRIMER_RIGHT_17_TM=58.920 PRIMER_LEFT_17_GC_PERCENT=47.619 PRIMER_RIGHT_17_GC_PERCENT=42.857 PRIMER_LEFT_17_SELF_ANY=4.00 PRIMER_RIGHT_17_SELF_ANY=4.00 PRIMER_LEFT_17_SELF_END=0.00 PRIMER_RIGHT_17_SELF_END=2.00 PRIMER_LEFT_17_POSITION_PENALTY=0.000000 PRIMER_RIGHT_17_POSITION_PENALTY=4.000000 PRIMER_LEFT_17_END_STABILITY=9.7000 PRIMER_RIGHT_17_END_STABILITY=8.4000 PRIMER_PAIR_17_COMPL_ANY=3.00 PRIMER_PAIR_17_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_17=960 PRIMER_PAIR_PENALTY_18=8.6491 PRIMER_LEFT_18_PENALTY=2.658657 PRIMER_RIGHT_18_PENALTY=5.990437 PRIMER_LEFT_18_SEQUENCE=ATGACTGAGCAGATGACCCTTC PRIMER_RIGHT_18_SEQUENCE=AACTTCTAGCGTGTGCCAAT PRIMER_LEFT_18=95,22 PRIMER_RIGHT_18=1053,20 PRIMER_LEFT_18_TM=60.659 PRIMER_RIGHT_18_TM=57.510 PRIMER_LEFT_18_GC_PERCENT=50.000 PRIMER_RIGHT_18_GC_PERCENT=45.000 PRIMER_LEFT_18_SELF_ANY=4.00 PRIMER_RIGHT_18_SELF_ANY=4.00 PRIMER_LEFT_18_SELF_END=1.00 PRIMER_RIGHT_18_SELF_END=2.00 PRIMER_LEFT_18_POSITION_PENALTY=0.000000 PRIMER_RIGHT_18_POSITION_PENALTY=3.500000 PRIMER_LEFT_18_END_STABILITY=8.2000 PRIMER_RIGHT_18_END_STABILITY=8.4000 PRIMER_PAIR_18_COMPL_ANY=3.00 PRIMER_PAIR_18_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_18=959 PRIMER_PAIR_PENALTY_19=8.6509 PRIMER_LEFT_19_PENALTY=2.658657 PRIMER_RIGHT_19_PENALTY=5.992277 PRIMER_LEFT_19_SEQUENCE=ATGACTGAGCAGATGACCCTTC PRIMER_RIGHT_19_SEQUENCE=CTTCTAGCGTGTGCCAATGGT PRIMER_LEFT_19=95,22 PRIMER_RIGHT_19=1051,21 PRIMER_LEFT_19_TM=60.659 PRIMER_RIGHT_19_TM=62.492 PRIMER_LEFT_19_GC_PERCENT=50.000 PRIMER_RIGHT_19_GC_PERCENT=52.381 PRIMER_LEFT_19_SELF_ANY=4.00 PRIMER_RIGHT_19_SELF_ANY=5.00 PRIMER_LEFT_19_SELF_END=1.00 PRIMER_RIGHT_19_SELF_END=3.00 PRIMER_LEFT_19_POSITION_PENALTY=0.000000 PRIMER_RIGHT_19_POSITION_PENALTY=2.500000 PRIMER_LEFT_19_END_STABILITY=8.2000 PRIMER_RIGHT_19_END_STABILITY=7.8000 PRIMER_PAIR_19_COMPL_ANY=4.00 PRIMER_PAIR_19_COMPL_END=3.00 PRIMER_PRODUCT_SIZE_19=957 = PRIMER_SEQUENCE_ID=X66357/CDK3 PRIMER_COMMENT=H.sapiens mRNA cdk3 for serine/threonine protein kinase. CDS=89..1006 PRIMER_START_CODON_POSITION=88 SEQUENCE=CCACATGGAAGCTGGAGGAGCAACCGGGAGCGCTGGGCTGGGGTGCAAATTGCCCAGTGCCTTCTGTTTCCCAGGCAGCTCTGTGGCCATGGATATGTTCCAGAAGGTAGAGAAGATCGGAGAGGGCACCTATGGGGTGGTGTACAAGGCCAAGAACAGGGAGACAGGGCAGCTGGTGGCCCTGAAGAAGATCAGACTGGATTTGGAGATGGAGGGGGTCCCAAGCACTGCCATCAGGGAGATCTCGCTGCTCAAGGAACTGAAGCACCCCAACATCGTCCGACTGCTGGACGTGGTGCACAACGAGAGGAAGCTCTATCTGGTGTTTGAGTTCCTCAGCCAGGACCTGAAGAAGTACATGGACTCCACCCCAGGCTCAGAGCTCCCCCTGCACCTCATCAAGAGCTACCTCTTCCAGCTGCTGCAGGGGGTGAGTTTCTGCCACTCACATCGGGTCATCCACCGAGACCTGAAGCCCCAGAACCTGCTCATCAATGAGTTGGGTGCCATCAAGCTGGCTGACTTCGGCCTGGCTCGCGCCTTCGGGGTGCCCCTGCGCACCTACACCCATGAGGTGGTGACACTGTGGTATCGCGCCCCCGAGATTCTCTTGGGCAGCAAGTTCTATACCACAGCTGTGGATATCTGGAGCATTGGTTGCATCTTTGCAGAGATGGTGACTCGAAAAGCCCTGTTTCCTGGTGACTCTGAGATTGACCAGCTCTTTCGTATCTTTCGTATGCTGGGGACACCCAGCGAAGACACATGGCCCGGGGTCACCCAGCTGCCTGACTATAAGGGCAGCTTCCCTAAGTGGACCAGGAAGGGACTGGAAGAGATTGTGCCCAATCTGGAGCCAGAGGGCAGGGACCTGCTCATGCAACTCCTGCAGTATGACCCCAGCCAGCGGATCACAGCCAAGACTGCCCTGGCCCACCCGTACTTCTCATCCCCTGAGCCCTCCCCAGCTGCCCGCCAGTATGTGCTGCAGCGATTCCGCCATTGAGAATGTCAAGGCCACACTCAGATCCTTTCTCGAGCAGCAGCTGCTGCCCCAGCTGCCTCCTACCCATTGCCAAGAGAGGATGCATCTGGGGAGAGCAAAGCACTAAGGAATTCAGCATCAGCCTGCAGAGGGCTGAGTCTGGGTTAGTCCTGCCC PRIMER_LEFT_EXPLAIN=considered 9505, would not amplify any of the ORF 6576, GC content failed 22, low tm 213, high tm 1911, high 3' stability 39, ok 744 PRIMER_RIGHT_EXPLAIN=considered 9326, GC content failed 54, low tm 682, high tm 6041, high any compl 1, high end compl 3, high 3' stability 58, ok 2487 PRIMER_PAIR_EXPLAIN=considered 22, ok 22 PRIMER_STOP_CODON_POSITION=1003 PRIMER_PAIR_PENALTY=9.6633 PRIMER_LEFT_PENALTY=5.563683 PRIMER_RIGHT_PENALTY=4.099578 PRIMER_LEFT_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAGA PRIMER_RIGHT_SEQUENCE=ACATTCTCAATGGCGGAATC PRIMER_LEFT=88,25 PRIMER_RIGHT=1011,20 PRIMER_LEFT_TM=59.436 PRIMER_RIGHT_TM=59.900 PRIMER_LEFT_GC_PERCENT=40.000 PRIMER_RIGHT_GC_PERCENT=45.000 PRIMER_LEFT_SELF_ANY=4.00 PRIMER_RIGHT_SELF_ANY=5.00 PRIMER_LEFT_SELF_END=0.00 PRIMER_RIGHT_SELF_END=3.00 PRIMER_LEFT_POSITION_PENALTY=0.000000 PRIMER_RIGHT_POSITION_PENALTY=4.000000 PRIMER_LEFT_END_STABILITY=6.4000 PRIMER_RIGHT_END_STABILITY=6.6000 PRIMER_PAIR_COMPL_ANY=5.00 PRIMER_PAIR_COMPL_END=2.00 PRIMER_PRODUCT_SIZE=924 PRIMER_PAIR_PENALTY_1=10.3311 PRIMER_LEFT_1_PENALTY=6.231558 PRIMER_RIGHT_1_PENALTY=4.099578 PRIMER_LEFT_1_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAG PRIMER_RIGHT_1_SEQUENCE=ACATTCTCAATGGCGGAATC PRIMER_LEFT_1=88,24 PRIMER_RIGHT_1=1011,20 PRIMER_LEFT_1_TM=57.768 PRIMER_RIGHT_1_TM=59.900 PRIMER_LEFT_1_GC_PERCENT=41.667 PRIMER_RIGHT_1_GC_PERCENT=45.000 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=5.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_LEFT_1_POSITION_PENALTY=0.000000 PRIMER_RIGHT_1_POSITION_PENALTY=4.000000 PRIMER_LEFT_1_END_STABILITY=5.7000 PRIMER_RIGHT_1_END_STABILITY=6.6000 PRIMER_PAIR_1_COMPL_ANY=5.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_1=924 PRIMER_PAIR_PENALTY_2=10.5213 PRIMER_LEFT_2_PENALTY=5.563683 PRIMER_RIGHT_2_PENALTY=4.957587 PRIMER_LEFT_2_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAGA PRIMER_RIGHT_2_SEQUENCE=ATTCTCAATGGCGGAATCG PRIMER_LEFT_2=88,25 PRIMER_RIGHT_2=1009,19 PRIMER_LEFT_2_TM=59.436 PRIMER_RIGHT_2_TM=60.958 PRIMER_LEFT_2_GC_PERCENT=40.000 PRIMER_RIGHT_2_GC_PERCENT=47.368 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=4.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_LEFT_2_POSITION_PENALTY=0.000000 PRIMER_RIGHT_2_POSITION_PENALTY=3.000000 PRIMER_LEFT_2_END_STABILITY=6.4000 PRIMER_RIGHT_2_END_STABILITY=8.6000 PRIMER_PAIR_2_COMPL_ANY=4.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_2=922 PRIMER_PAIR_PENALTY_3=10.6709 PRIMER_LEFT_3_PENALTY=6.571364 PRIMER_RIGHT_3_PENALTY=4.099578 PRIMER_LEFT_3_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAGAA PRIMER_RIGHT_3_SEQUENCE=ACATTCTCAATGGCGGAATC PRIMER_LEFT_3=88,26 PRIMER_RIGHT_3=1011,20 PRIMER_LEFT_3_TM=60.571 PRIMER_RIGHT_3_TM=59.900 PRIMER_LEFT_3_GC_PERCENT=38.462 PRIMER_RIGHT_3_GC_PERCENT=45.000 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=5.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=3.00 PRIMER_LEFT_3_POSITION_PENALTY=0.000000 PRIMER_RIGHT_3_POSITION_PENALTY=4.000000 PRIMER_LEFT_3_END_STABILITY=6.7000 PRIMER_RIGHT_3_END_STABILITY=6.6000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_3=924 PRIMER_PAIR_PENALTY_4=11.0485 PRIMER_LEFT_4_PENALTY=5.563683 PRIMER_RIGHT_4_PENALTY=5.484828 PRIMER_LEFT_4_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAGA PRIMER_RIGHT_4_SEQUENCE=CATTCTCAATGGCGGAATC PRIMER_LEFT_4=88,25 PRIMER_RIGHT_4=1010,19 PRIMER_LEFT_4_TM=59.436 PRIMER_RIGHT_4_TM=59.015 PRIMER_LEFT_4_GC_PERCENT=40.000 PRIMER_RIGHT_4_GC_PERCENT=47.368 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=5.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=3.00 PRIMER_LEFT_4_POSITION_PENALTY=0.000000 PRIMER_RIGHT_4_POSITION_PENALTY=3.500000 PRIMER_LEFT_4_END_STABILITY=6.4000 PRIMER_RIGHT_4_END_STABILITY=6.6000 PRIMER_PAIR_4_COMPL_ANY=4.00 PRIMER_PAIR_4_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_4=923 PRIMER_PAIR_PENALTY_5=11.1891 PRIMER_LEFT_5_PENALTY=6.231558 PRIMER_RIGHT_5_PENALTY=4.957587 PRIMER_LEFT_5_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAG PRIMER_RIGHT_5_SEQUENCE=ATTCTCAATGGCGGAATCG PRIMER_LEFT_5=88,24 PRIMER_RIGHT_5=1009,19 PRIMER_LEFT_5_TM=57.768 PRIMER_RIGHT_5_TM=60.958 PRIMER_LEFT_5_GC_PERCENT=41.667 PRIMER_RIGHT_5_GC_PERCENT=47.368 PRIMER_LEFT_5_SELF_ANY=4.00 PRIMER_RIGHT_5_SELF_ANY=4.00 PRIMER_LEFT_5_SELF_END=0.00 PRIMER_RIGHT_5_SELF_END=2.00 PRIMER_LEFT_5_POSITION_PENALTY=0.000000 PRIMER_RIGHT_5_POSITION_PENALTY=3.000000 PRIMER_LEFT_5_END_STABILITY=5.7000 PRIMER_RIGHT_5_END_STABILITY=8.6000 PRIMER_PAIR_5_COMPL_ANY=4.00 PRIMER_PAIR_5_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_5=922 PRIMER_PAIR_PENALTY_6=11.5290 PRIMER_LEFT_6_PENALTY=6.571364 PRIMER_RIGHT_6_PENALTY=4.957587 PRIMER_LEFT_6_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAGAA PRIMER_RIGHT_6_SEQUENCE=ATTCTCAATGGCGGAATCG PRIMER_LEFT_6=88,26 PRIMER_RIGHT_6=1009,19 PRIMER_LEFT_6_TM=60.571 PRIMER_RIGHT_6_TM=60.958 PRIMER_LEFT_6_GC_PERCENT=38.462 PRIMER_RIGHT_6_GC_PERCENT=47.368 PRIMER_LEFT_6_SELF_ANY=4.00 PRIMER_RIGHT_6_SELF_ANY=4.00 PRIMER_LEFT_6_SELF_END=0.00 PRIMER_RIGHT_6_SELF_END=2.00 PRIMER_LEFT_6_POSITION_PENALTY=0.000000 PRIMER_RIGHT_6_POSITION_PENALTY=3.000000 PRIMER_LEFT_6_END_STABILITY=6.7000 PRIMER_RIGHT_6_END_STABILITY=8.6000 PRIMER_PAIR_6_COMPL_ANY=5.00 PRIMER_PAIR_6_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_6=922 PRIMER_PAIR_PENALTY_7=11.7164 PRIMER_LEFT_7_PENALTY=6.231558 PRIMER_RIGHT_7_PENALTY=5.484828 PRIMER_LEFT_7_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAG PRIMER_RIGHT_7_SEQUENCE=CATTCTCAATGGCGGAATC PRIMER_LEFT_7=88,24 PRIMER_RIGHT_7=1010,19 PRIMER_LEFT_7_TM=57.768 PRIMER_RIGHT_7_TM=59.015 PRIMER_LEFT_7_GC_PERCENT=41.667 PRIMER_RIGHT_7_GC_PERCENT=47.368 PRIMER_LEFT_7_SELF_ANY=4.00 PRIMER_RIGHT_7_SELF_ANY=5.00 PRIMER_LEFT_7_SELF_END=0.00 PRIMER_RIGHT_7_SELF_END=3.00 PRIMER_LEFT_7_POSITION_PENALTY=0.000000 PRIMER_RIGHT_7_POSITION_PENALTY=3.500000 PRIMER_LEFT_7_END_STABILITY=5.7000 PRIMER_RIGHT_7_END_STABILITY=6.6000 PRIMER_PAIR_7_COMPL_ANY=4.00 PRIMER_PAIR_7_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_7=923 PRIMER_PAIR_PENALTY_8=12.0562 PRIMER_LEFT_8_PENALTY=6.571364 PRIMER_RIGHT_8_PENALTY=5.484828 PRIMER_LEFT_8_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAGAA PRIMER_RIGHT_8_SEQUENCE=CATTCTCAATGGCGGAATC PRIMER_LEFT_8=88,26 PRIMER_RIGHT_8=1010,19 PRIMER_LEFT_8_TM=60.571 PRIMER_RIGHT_8_TM=59.015 PRIMER_LEFT_8_GC_PERCENT=38.462 PRIMER_RIGHT_8_GC_PERCENT=47.368 PRIMER_LEFT_8_SELF_ANY=4.00 PRIMER_RIGHT_8_SELF_ANY=5.00 PRIMER_LEFT_8_SELF_END=0.00 PRIMER_RIGHT_8_SELF_END=3.00 PRIMER_LEFT_8_POSITION_PENALTY=0.000000 PRIMER_RIGHT_8_POSITION_PENALTY=3.500000 PRIMER_LEFT_8_END_STABILITY=6.7000 PRIMER_RIGHT_8_END_STABILITY=6.6000 PRIMER_PAIR_8_COMPL_ANY=5.00 PRIMER_PAIR_8_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_8=923 PRIMER_PAIR_PENALTY_9=12.4165 PRIMER_LEFT_9_PENALTY=8.316960 PRIMER_RIGHT_9_PENALTY=4.099578 PRIMER_LEFT_9_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAGAAG PRIMER_RIGHT_9_SEQUENCE=ACATTCTCAATGGCGGAATC PRIMER_LEFT_9=88,27 PRIMER_RIGHT_9=1011,20 PRIMER_LEFT_9_TM=61.317 PRIMER_RIGHT_9_TM=59.900 PRIMER_LEFT_9_GC_PERCENT=40.741 PRIMER_RIGHT_9_GC_PERCENT=45.000 PRIMER_LEFT_9_SELF_ANY=4.00 PRIMER_RIGHT_9_SELF_ANY=5.00 PRIMER_LEFT_9_SELF_END=0.00 PRIMER_RIGHT_9_SELF_END=3.00 PRIMER_LEFT_9_POSITION_PENALTY=0.000000 PRIMER_RIGHT_9_POSITION_PENALTY=4.000000 PRIMER_LEFT_9_END_STABILITY=6.7000 PRIMER_RIGHT_9_END_STABILITY=6.6000 PRIMER_PAIR_9_COMPL_ANY=5.00 PRIMER_PAIR_9_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_9=924 PRIMER_PAIR_PENALTY_10=12.6695 PRIMER_LEFT_10_PENALTY=5.563683 PRIMER_RIGHT_10_PENALTY=7.105834 PRIMER_LEFT_10_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAGA PRIMER_RIGHT_10_SEQUENCE=TCTCAATGGCGGAATCGCT PRIMER_LEFT_10=88,25 PRIMER_RIGHT_10=1007,19 PRIMER_LEFT_10_TM=59.436 PRIMER_RIGHT_10_TM=64.106 PRIMER_LEFT_10_GC_PERCENT=40.000 PRIMER_RIGHT_10_GC_PERCENT=52.632 PRIMER_LEFT_10_SELF_ANY=4.00 PRIMER_RIGHT_10_SELF_ANY=3.00 PRIMER_LEFT_10_SELF_END=0.00 PRIMER_RIGHT_10_SELF_END=2.00 PRIMER_LEFT_10_POSITION_PENALTY=0.000000 PRIMER_RIGHT_10_POSITION_PENALTY=2.000000 PRIMER_LEFT_10_END_STABILITY=6.4000 PRIMER_RIGHT_10_END_STABILITY=9.9000 PRIMER_PAIR_10_COMPL_ANY=4.00 PRIMER_PAIR_10_COMPL_END=3.00 PRIMER_PRODUCT_SIZE_10=920 PRIMER_PAIR_PENALTY_11=12.7766 PRIMER_LEFT_11_PENALTY=5.563683 PRIMER_RIGHT_11_PENALTY=7.212929 PRIMER_LEFT_11_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAGA PRIMER_RIGHT_11_SEQUENCE=CATTCTCAATGGCGGAATCG PRIMER_LEFT_11=88,25 PRIMER_RIGHT_11=1010,20 PRIMER_LEFT_11_TM=59.436 PRIMER_RIGHT_11_TM=63.713 PRIMER_LEFT_11_GC_PERCENT=40.000 PRIMER_RIGHT_11_GC_PERCENT=50.000 PRIMER_LEFT_11_SELF_ANY=4.00 PRIMER_RIGHT_11_SELF_ANY=5.00 PRIMER_LEFT_11_SELF_END=0.00 PRIMER_RIGHT_11_SELF_END=2.00 PRIMER_LEFT_11_POSITION_PENALTY=0.000000 PRIMER_RIGHT_11_POSITION_PENALTY=3.500000 PRIMER_LEFT_11_END_STABILITY=6.4000 PRIMER_RIGHT_11_END_STABILITY=8.6000 PRIMER_PAIR_11_COMPL_ANY=4.00 PRIMER_PAIR_11_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_11=923 PRIMER_PAIR_PENALTY_12=12.7991 PRIMER_LEFT_12_PENALTY=5.563683 PRIMER_RIGHT_12_PENALTY=7.235377 PRIMER_LEFT_12_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAGA PRIMER_RIGHT_12_SEQUENCE=GACATTCTCAATGGCGGAATC PRIMER_LEFT_12=88,25 PRIMER_RIGHT_12=1012,21 PRIMER_LEFT_12_TM=59.436 PRIMER_RIGHT_12_TM=61.735 PRIMER_LEFT_12_GC_PERCENT=40.000 PRIMER_RIGHT_12_GC_PERCENT=47.619 PRIMER_LEFT_12_SELF_ANY=4.00 PRIMER_RIGHT_12_SELF_ANY=5.00 PRIMER_LEFT_12_SELF_END=0.00 PRIMER_RIGHT_12_SELF_END=3.00 PRIMER_LEFT_12_POSITION_PENALTY=0.000000 PRIMER_RIGHT_12_POSITION_PENALTY=4.500000 PRIMER_LEFT_12_END_STABILITY=6.4000 PRIMER_RIGHT_12_END_STABILITY=6.6000 PRIMER_PAIR_12_COMPL_ANY=5.00 PRIMER_PAIR_12_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_12=925 PRIMER_PAIR_PENALTY_13=13.0556 PRIMER_LEFT_13_PENALTY=5.563683 PRIMER_RIGHT_13_PENALTY=7.491945 PRIMER_LEFT_13_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAGA PRIMER_RIGHT_13_SEQUENCE=ATTCTCAATGGCGGAATCGC PRIMER_LEFT_13=88,25 PRIMER_RIGHT_13=1009,20 PRIMER_LEFT_13_TM=59.436 PRIMER_RIGHT_13_TM=64.492 PRIMER_LEFT_13_GC_PERCENT=40.000 PRIMER_RIGHT_13_GC_PERCENT=50.000 PRIMER_LEFT_13_SELF_ANY=4.00 PRIMER_RIGHT_13_SELF_ANY=4.00 PRIMER_LEFT_13_SELF_END=0.00 PRIMER_RIGHT_13_SELF_END=3.00 PRIMER_LEFT_13_POSITION_PENALTY=0.000000 PRIMER_RIGHT_13_POSITION_PENALTY=3.000000 PRIMER_LEFT_13_END_STABILITY=6.4000 PRIMER_RIGHT_13_END_STABILITY=9.8000 PRIMER_PAIR_13_COMPL_ANY=4.00 PRIMER_PAIR_13_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_13=922 PRIMER_PAIR_PENALTY_14=13.2745 PRIMER_LEFT_14_PENALTY=8.316960 PRIMER_RIGHT_14_PENALTY=4.957587 PRIMER_LEFT_14_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAGAAG PRIMER_RIGHT_14_SEQUENCE=ATTCTCAATGGCGGAATCG PRIMER_LEFT_14=88,27 PRIMER_RIGHT_14=1009,19 PRIMER_LEFT_14_TM=61.317 PRIMER_RIGHT_14_TM=60.958 PRIMER_LEFT_14_GC_PERCENT=40.741 PRIMER_RIGHT_14_GC_PERCENT=47.368 PRIMER_LEFT_14_SELF_ANY=4.00 PRIMER_RIGHT_14_SELF_ANY=4.00 PRIMER_LEFT_14_SELF_END=0.00 PRIMER_RIGHT_14_SELF_END=2.00 PRIMER_LEFT_14_POSITION_PENALTY=0.000000 PRIMER_RIGHT_14_POSITION_PENALTY=3.000000 PRIMER_LEFT_14_END_STABILITY=6.7000 PRIMER_RIGHT_14_END_STABILITY=8.6000 PRIMER_PAIR_14_COMPL_ANY=5.00 PRIMER_PAIR_14_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_14=922 PRIMER_PAIR_PENALTY_15=13.3374 PRIMER_LEFT_15_PENALTY=6.231558 PRIMER_RIGHT_15_PENALTY=7.105834 PRIMER_LEFT_15_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAG PRIMER_RIGHT_15_SEQUENCE=TCTCAATGGCGGAATCGCT PRIMER_LEFT_15=88,24 PRIMER_RIGHT_15=1007,19 PRIMER_LEFT_15_TM=57.768 PRIMER_RIGHT_15_TM=64.106 PRIMER_LEFT_15_GC_PERCENT=41.667 PRIMER_RIGHT_15_GC_PERCENT=52.632 PRIMER_LEFT_15_SELF_ANY=4.00 PRIMER_RIGHT_15_SELF_ANY=3.00 PRIMER_LEFT_15_SELF_END=0.00 PRIMER_RIGHT_15_SELF_END=2.00 PRIMER_LEFT_15_POSITION_PENALTY=0.000000 PRIMER_RIGHT_15_POSITION_PENALTY=2.000000 PRIMER_LEFT_15_END_STABILITY=5.7000 PRIMER_RIGHT_15_END_STABILITY=9.9000 PRIMER_PAIR_15_COMPL_ANY=4.00 PRIMER_PAIR_15_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_15=920 PRIMER_PAIR_PENALTY_16=13.4445 PRIMER_LEFT_16_PENALTY=6.231558 PRIMER_RIGHT_16_PENALTY=7.212929 PRIMER_LEFT_16_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAG PRIMER_RIGHT_16_SEQUENCE=CATTCTCAATGGCGGAATCG PRIMER_LEFT_16=88,24 PRIMER_RIGHT_16=1010,20 PRIMER_LEFT_16_TM=57.768 PRIMER_RIGHT_16_TM=63.713 PRIMER_LEFT_16_GC_PERCENT=41.667 PRIMER_RIGHT_16_GC_PERCENT=50.000 PRIMER_LEFT_16_SELF_ANY=4.00 PRIMER_RIGHT_16_SELF_ANY=5.00 PRIMER_LEFT_16_SELF_END=0.00 PRIMER_RIGHT_16_SELF_END=2.00 PRIMER_LEFT_16_POSITION_PENALTY=0.000000 PRIMER_RIGHT_16_POSITION_PENALTY=3.500000 PRIMER_LEFT_16_END_STABILITY=5.7000 PRIMER_RIGHT_16_END_STABILITY=8.6000 PRIMER_PAIR_16_COMPL_ANY=4.00 PRIMER_PAIR_16_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_16=923 PRIMER_PAIR_PENALTY_17=13.4669 PRIMER_LEFT_17_PENALTY=6.231558 PRIMER_RIGHT_17_PENALTY=7.235377 PRIMER_LEFT_17_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAG PRIMER_RIGHT_17_SEQUENCE=GACATTCTCAATGGCGGAATC PRIMER_LEFT_17=88,24 PRIMER_RIGHT_17=1012,21 PRIMER_LEFT_17_TM=57.768 PRIMER_RIGHT_17_TM=61.735 PRIMER_LEFT_17_GC_PERCENT=41.667 PRIMER_RIGHT_17_GC_PERCENT=47.619 PRIMER_LEFT_17_SELF_ANY=4.00 PRIMER_RIGHT_17_SELF_ANY=5.00 PRIMER_LEFT_17_SELF_END=0.00 PRIMER_RIGHT_17_SELF_END=3.00 PRIMER_LEFT_17_POSITION_PENALTY=0.000000 PRIMER_RIGHT_17_POSITION_PENALTY=4.500000 PRIMER_LEFT_17_END_STABILITY=5.7000 PRIMER_RIGHT_17_END_STABILITY=6.6000 PRIMER_PAIR_17_COMPL_ANY=5.00 PRIMER_PAIR_17_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_17=925 PRIMER_PAIR_PENALTY_18=13.5226 PRIMER_LEFT_18_PENALTY=5.563683 PRIMER_RIGHT_18_PENALTY=7.958919 PRIMER_LEFT_18_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAGA PRIMER_RIGHT_18_SEQUENCE=TTCTCAATGGCGGAATCGC PRIMER_LEFT_18=88,25 PRIMER_RIGHT_18=1008,19 PRIMER_LEFT_18_TM=59.436 PRIMER_RIGHT_18_TM=64.459 PRIMER_LEFT_18_GC_PERCENT=40.000 PRIMER_RIGHT_18_GC_PERCENT=52.632 PRIMER_LEFT_18_SELF_ANY=4.00 PRIMER_RIGHT_18_SELF_ANY=3.00 PRIMER_LEFT_18_SELF_END=0.00 PRIMER_RIGHT_18_SELF_END=3.00 PRIMER_LEFT_18_POSITION_PENALTY=0.000000 PRIMER_RIGHT_18_POSITION_PENALTY=2.500000 PRIMER_LEFT_18_END_STABILITY=6.4000 PRIMER_RIGHT_18_END_STABILITY=9.8000 PRIMER_PAIR_18_COMPL_ANY=4.00 PRIMER_PAIR_18_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_18=921 PRIMER_PAIR_PENALTY_19=13.6772 PRIMER_LEFT_19_PENALTY=6.571364 PRIMER_RIGHT_19_PENALTY=7.105834 PRIMER_LEFT_19_SEQUENCE=ATGGATATGTTCCAGAAGGTAGAGAA PRIMER_RIGHT_19_SEQUENCE=TCTCAATGGCGGAATCGCT PRIMER_LEFT_19=88,26 PRIMER_RIGHT_19=1007,19 PRIMER_LEFT_19_TM=60.571 PRIMER_RIGHT_19_TM=64.106 PRIMER_LEFT_19_GC_PERCENT=38.462 PRIMER_RIGHT_19_GC_PERCENT=52.632 PRIMER_LEFT_19_SELF_ANY=4.00 PRIMER_RIGHT_19_SELF_ANY=3.00 PRIMER_LEFT_19_SELF_END=0.00 PRIMER_RIGHT_19_SELF_END=2.00 PRIMER_LEFT_19_POSITION_PENALTY=0.000000 PRIMER_RIGHT_19_POSITION_PENALTY=2.000000 PRIMER_LEFT_19_END_STABILITY=6.7000 PRIMER_RIGHT_19_END_STABILITY=9.9000 PRIMER_PAIR_19_COMPL_ANY=4.00 PRIMER_PAIR_19_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_19=920 = PRIMER_SEQUENCE_ID=X66364/CDK5 PRIMER_COMMENT=H.sapiens mRNA PSSALRE for serine/threonine protein kinase. CDS=25..903 PRIMER_START_CODON_POSITION=24 SEQUENCE=CGCAGGGGTCCCCCGGCCGCCGCGATGCAGAAATACGAGAAACTGGAAAAGATTGGGGAAGGCACCTACGGAACTGTGTTCAAGGCCAAAAACCGGGAGACTCATGAGATCGTGGCTCTGAAACGGGTGAGGCTGGATGACGATGATGAGGGTGTGCCGAGTTCCGCCCTCCGGGAGATCTGCCTACTCAAGGAGCTGAAGCACAAGAACATCGTCAGGCTTCATGACGTCCTGCACAGCGACAAGAAGCTGACTTTGGTTTTTGAATTCTGTGACCAGGACCTGAAGAAGTATTTTGACAGTTGCAATGGTGACCTCGATCCTGAGATTGTAAAGTCATTCCTCTTCCAGCTACTAAAAGGGCTGGGATTCTGTCATAGCCGCAATGTGCTACACAGGGACCTGAAGCCCCAGAACCTGCTAATAAACAGGAATGGGGAGCTGAAATTGGCTGATTTTGGCCTGGCTCGAGCCTTTGGGATTCCCGTCCGCTGTTACTCAGCTGAGGTGGTCACACTGTGGTACCGCCCACCGGATGTCCTCTTTGGGGCCAAGCTGTACTCCACGTCCATCGACATGTGGTCAGCCGGCTGCATCTTTGCAGAGCTGGCCAATGCTGGGCGGCCTCTTTTTCCCGGCAATGATGTCGATGACCAGTTGAAGAGGATCTTCCGACTGCTGGGGACGCCCACCGAGGAGCAGTGGCCCTCTATGACCAAGCTGCCAGACTATAAGCCCTATCCGATGTACCCGGCCACAACATCCCTGGTGAACGTCGTGCCCAAACTCAATGCCACAGGGAGGGATCTGCTGCAGAACCTTCTGAAGTGTAACCCTGTCCAGCGTATCTCAGCAGAAGAGGCCCTGCAGCACCCCTACTTCTCCGACTTCTGTCCGCCCTAGGCCCCGGGACCCCCGCCTCCAGGCTGGGCCTGGCCTATTTAAGCCCCCTCTTGAGAGGGTGAGACAGTGGGGGTGCCTGG PRIMER_LEFT_EXPLAIN=considered 7904, would not amplify any of the ORF 5278, GC content failed 48, low tm 249, high tm 1503, high 3' stability 40, ok 786 PRIMER_RIGHT_EXPLAIN=considered 7709, GC content failed 194, low tm 667, high tm 4515, high 3' stability 60, ok 2273 PRIMER_PAIR_EXPLAIN=considered 21, ok 21 PRIMER_STOP_CODON_POSITION=900 PRIMER_PAIR_PENALTY=5.8309 PRIMER_LEFT_PENALTY=3.142611 PRIMER_RIGHT_PENALTY=2.688260 PRIMER_LEFT_SEQUENCE=ATGCAGAAATACGAGAAACTGGA PRIMER_RIGHT_SEQUENCE=CCTAGGGCGGACAGAAGTC PRIMER_LEFT=24,23 PRIMER_RIGHT=903,19 PRIMER_LEFT_TM=60.143 PRIMER_RIGHT_TM=59.812 PRIMER_LEFT_GC_PERCENT=39.130 PRIMER_RIGHT_GC_PERCENT=63.158 PRIMER_LEFT_SELF_ANY=4.00 PRIMER_RIGHT_SELF_ANY=6.00 PRIMER_LEFT_SELF_END=0.00 PRIMER_RIGHT_SELF_END=2.00 PRIMER_LEFT_POSITION_PENALTY=0.000000 PRIMER_RIGHT_POSITION_PENALTY=1.500000 PRIMER_LEFT_END_STABILITY=8.2000 PRIMER_RIGHT_END_STABILITY=6.4000 PRIMER_PAIR_COMPL_ANY=3.00 PRIMER_PAIR_COMPL_END=2.00 PRIMER_PRODUCT_SIZE=880 PRIMER_PAIR_PENALTY_1=6.0811 PRIMER_LEFT_1_PENALTY=3.142611 PRIMER_RIGHT_1_PENALTY=2.938464 PRIMER_LEFT_1_SEQUENCE=ATGCAGAAATACGAGAAACTGGA PRIMER_RIGHT_1_SEQUENCE=CTAGGGCGGACAGAAGTCG PRIMER_LEFT_1=24,23 PRIMER_RIGHT_1=902,19 PRIMER_LEFT_1_TM=60.143 PRIMER_RIGHT_1_TM=60.938 PRIMER_LEFT_1_GC_PERCENT=39.130 PRIMER_RIGHT_1_GC_PERCENT=63.158 PRIMER_LEFT_1_SELF_ANY=4.00 PRIMER_RIGHT_1_SELF_ANY=4.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=2.00 PRIMER_LEFT_1_POSITION_PENALTY=0.000000 PRIMER_RIGHT_1_POSITION_PENALTY=1.000000 PRIMER_LEFT_1_END_STABILITY=8.2000 PRIMER_RIGHT_1_END_STABILITY=8.1000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_1=879 PRIMER_PAIR_PENALTY_2=6.2856 PRIMER_LEFT_2_PENALTY=3.597306 PRIMER_RIGHT_2_PENALTY=2.688260 PRIMER_LEFT_2_SEQUENCE=ATGCAGAAATACGAGAAACTGG PRIMER_RIGHT_2_SEQUENCE=CCTAGGGCGGACAGAAGTC PRIMER_LEFT_2=24,22 PRIMER_RIGHT_2=903,19 PRIMER_LEFT_2_TM=58.403 PRIMER_RIGHT_2_TM=59.812 PRIMER_LEFT_2_GC_PERCENT=40.909 PRIMER_RIGHT_2_GC_PERCENT=63.158 PRIMER_LEFT_2_SELF_ANY=4.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=1.00 PRIMER_RIGHT_2_SELF_END=2.00 PRIMER_LEFT_2_POSITION_PENALTY=0.000000 PRIMER_RIGHT_2_POSITION_PENALTY=1.500000 PRIMER_LEFT_2_END_STABILITY=7.9000 PRIMER_RIGHT_2_END_STABILITY=6.4000 PRIMER_PAIR_2_COMPL_ANY=3.00 PRIMER_PAIR_2_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_2=880 PRIMER_PAIR_PENALTY_3=6.5358 PRIMER_LEFT_3_PENALTY=3.597306 PRIMER_RIGHT_3_PENALTY=2.938464 PRIMER_LEFT_3_SEQUENCE=ATGCAGAAATACGAGAAACTGG PRIMER_RIGHT_3_SEQUENCE=CTAGGGCGGACAGAAGTCG PRIMER_LEFT_3=24,22 PRIMER_RIGHT_3=902,19 PRIMER_LEFT_3_TM=58.403 PRIMER_RIGHT_3_TM=60.938 PRIMER_LEFT_3_GC_PERCENT=40.909 PRIMER_RIGHT_3_GC_PERCENT=63.158 PRIMER_LEFT_3_SELF_ANY=4.00 PRIMER_RIGHT_3_SELF_ANY=4.00 PRIMER_LEFT_3_SELF_END=1.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_LEFT_3_POSITION_PENALTY=0.000000 PRIMER_RIGHT_3_POSITION_PENALTY=1.000000 PRIMER_LEFT_3_END_STABILITY=7.9000 PRIMER_RIGHT_3_END_STABILITY=8.1000 PRIMER_PAIR_3_COMPL_ANY=3.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_3=879 PRIMER_PAIR_PENALTY_4=7.8470 PRIMER_LEFT_4_PENALTY=3.142611 PRIMER_RIGHT_4_PENALTY=4.704404 PRIMER_LEFT_4_SEQUENCE=ATGCAGAAATACGAGAAACTGGA PRIMER_RIGHT_4_SEQUENCE=GCCTAGGGCGGACAGAAGT PRIMER_LEFT_4=24,23 PRIMER_RIGHT_4=904,19 PRIMER_LEFT_4_TM=60.143 PRIMER_RIGHT_4_TM=61.704 PRIMER_LEFT_4_GC_PERCENT=39.130 PRIMER_RIGHT_4_GC_PERCENT=63.158 PRIMER_LEFT_4_SELF_ANY=4.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=1.00 PRIMER_LEFT_4_POSITION_PENALTY=0.000000 PRIMER_RIGHT_4_POSITION_PENALTY=2.000000 PRIMER_LEFT_4_END_STABILITY=8.2000 PRIMER_RIGHT_4_END_STABILITY=6.4000 PRIMER_PAIR_4_COMPL_ANY=3.00 PRIMER_PAIR_4_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_4=881 PRIMER_PAIR_PENALTY_5=7.9905 PRIMER_LEFT_5_PENALTY=5.302203 PRIMER_RIGHT_5_PENALTY=2.688260 PRIMER_LEFT_5_SEQUENCE=ATGCAGAAATACGAGAAACTGGAA PRIMER_RIGHT_5_SEQUENCE=CCTAGGGCGGACAGAAGTC PRIMER_LEFT_5=24,24 PRIMER_RIGHT_5=903,19 PRIMER_LEFT_5_TM=61.302 PRIMER_RIGHT_5_TM=59.812 PRIMER_LEFT_5_GC_PERCENT=37.500 PRIMER_RIGHT_5_GC_PERCENT=63.158 PRIMER_LEFT_5_SELF_ANY=4.00 PRIMER_RIGHT_5_SELF_ANY=6.00 PRIMER_LEFT_5_SELF_END=1.00 PRIMER_RIGHT_5_SELF_END=2.00 PRIMER_LEFT_5_POSITION_PENALTY=0.000000 PRIMER_RIGHT_5_POSITION_PENALTY=1.500000 PRIMER_LEFT_5_END_STABILITY=8.5000 PRIMER_RIGHT_5_END_STABILITY=6.4000 PRIMER_PAIR_5_COMPL_ANY=3.00 PRIMER_PAIR_5_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_5=880 PRIMER_PAIR_PENALTY_6=8.2407 PRIMER_LEFT_6_PENALTY=5.302203 PRIMER_RIGHT_6_PENALTY=2.938464 PRIMER_LEFT_6_SEQUENCE=ATGCAGAAATACGAGAAACTGGAA PRIMER_RIGHT_6_SEQUENCE=CTAGGGCGGACAGAAGTCG PRIMER_LEFT_6=24,24 PRIMER_RIGHT_6=902,19 PRIMER_LEFT_6_TM=61.302 PRIMER_RIGHT_6_TM=60.938 PRIMER_LEFT_6_GC_PERCENT=37.500 PRIMER_RIGHT_6_GC_PERCENT=63.158 PRIMER_LEFT_6_SELF_ANY=4.00 PRIMER_RIGHT_6_SELF_ANY=4.00 PRIMER_LEFT_6_SELF_END=1.00 PRIMER_RIGHT_6_SELF_END=2.00 PRIMER_LEFT_6_POSITION_PENALTY=0.000000 PRIMER_RIGHT_6_POSITION_PENALTY=1.000000 PRIMER_LEFT_6_END_STABILITY=8.5000 PRIMER_RIGHT_6_END_STABILITY=8.1000 PRIMER_PAIR_6_COMPL_ANY=3.00 PRIMER_PAIR_6_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_6=879 PRIMER_PAIR_PENALTY_7=8.2442 PRIMER_LEFT_7_PENALTY=3.142611 PRIMER_RIGHT_7_PENALTY=5.101542 PRIMER_LEFT_7_SEQUENCE=ATGCAGAAATACGAGAAACTGGA PRIMER_RIGHT_7_SEQUENCE=TAGGGCGGACAGAAGTCGG PRIMER_LEFT_7=24,23 PRIMER_RIGHT_7=901,19 PRIMER_LEFT_7_TM=60.143 PRIMER_RIGHT_7_TM=63.602 PRIMER_LEFT_7_GC_PERCENT=39.130 PRIMER_RIGHT_7_GC_PERCENT=63.158 PRIMER_LEFT_7_SELF_ANY=4.00 PRIMER_RIGHT_7_SELF_ANY=3.00 PRIMER_LEFT_7_SELF_END=0.00 PRIMER_RIGHT_7_SELF_END=2.00 PRIMER_LEFT_7_POSITION_PENALTY=0.000000 PRIMER_RIGHT_7_POSITION_PENALTY=0.500000 PRIMER_LEFT_7_END_STABILITY=8.2000 PRIMER_RIGHT_7_END_STABILITY=9.6000 PRIMER_PAIR_7_COMPL_ANY=3.00 PRIMER_PAIR_7_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_7=878 PRIMER_PAIR_PENALTY_8=8.3017 PRIMER_LEFT_8_PENALTY=3.597306 PRIMER_RIGHT_8_PENALTY=4.704404 PRIMER_LEFT_8_SEQUENCE=ATGCAGAAATACGAGAAACTGG PRIMER_RIGHT_8_SEQUENCE=GCCTAGGGCGGACAGAAGT PRIMER_LEFT_8=24,22 PRIMER_RIGHT_8=904,19 PRIMER_LEFT_8_TM=58.403 PRIMER_RIGHT_8_TM=61.704 PRIMER_LEFT_8_GC_PERCENT=40.909 PRIMER_RIGHT_8_GC_PERCENT=63.158 PRIMER_LEFT_8_SELF_ANY=4.00 PRIMER_RIGHT_8_SELF_ANY=6.00 PRIMER_LEFT_8_SELF_END=1.00 PRIMER_RIGHT_8_SELF_END=1.00 PRIMER_LEFT_8_POSITION_PENALTY=0.000000 PRIMER_RIGHT_8_POSITION_PENALTY=2.000000 PRIMER_LEFT_8_END_STABILITY=7.9000 PRIMER_RIGHT_8_END_STABILITY=6.4000 PRIMER_PAIR_8_COMPL_ANY=3.00 PRIMER_PAIR_8_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_8=881 PRIMER_PAIR_PENALTY_9=8.5177 PRIMER_LEFT_9_PENALTY=3.142611 PRIMER_RIGHT_9_PENALTY=5.375058 PRIMER_LEFT_9_SEQUENCE=ATGCAGAAATACGAGAAACTGGA PRIMER_RIGHT_9_SEQUENCE=CTAGGGCGGACAGAAGTCGG PRIMER_LEFT_9=24,23 PRIMER_RIGHT_9=902,20 PRIMER_LEFT_9_TM=60.143 PRIMER_RIGHT_9_TM=64.375 PRIMER_LEFT_9_GC_PERCENT=39.130 PRIMER_RIGHT_9_GC_PERCENT=65.000 PRIMER_LEFT_9_SELF_ANY=4.00 PRIMER_RIGHT_9_SELF_ANY=4.00 PRIMER_LEFT_9_SELF_END=0.00 PRIMER_RIGHT_9_SELF_END=2.00 PRIMER_LEFT_9_POSITION_PENALTY=0.000000 PRIMER_RIGHT_9_POSITION_PENALTY=1.000000 PRIMER_LEFT_9_END_STABILITY=8.2000 PRIMER_RIGHT_9_END_STABILITY=9.6000 PRIMER_PAIR_9_COMPL_ANY=3.00 PRIMER_PAIR_9_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_9=879 PRIMER_PAIR_PENALTY_10=8.6110 PRIMER_LEFT_10_PENALTY=3.142611 PRIMER_RIGHT_10_PENALTY=5.468402 PRIMER_LEFT_10_SEQUENCE=ATGCAGAAATACGAGAAACTGGA PRIMER_RIGHT_10_SEQUENCE=GCCTAGGGCGGACAGAAGTC PRIMER_LEFT_10=24,23 PRIMER_RIGHT_10=904,20 PRIMER_LEFT_10_TM=60.143 PRIMER_RIGHT_10_TM=63.468 PRIMER_LEFT_10_GC_PERCENT=39.130 PRIMER_RIGHT_10_GC_PERCENT=65.000 PRIMER_LEFT_10_SELF_ANY=4.00 PRIMER_RIGHT_10_SELF_ANY=6.00 PRIMER_LEFT_10_SELF_END=0.00 PRIMER_RIGHT_10_SELF_END=2.00 PRIMER_LEFT_10_POSITION_PENALTY=0.000000 PRIMER_RIGHT_10_POSITION_PENALTY=2.000000 PRIMER_LEFT_10_END_STABILITY=8.2000 PRIMER_RIGHT_10_END_STABILITY=6.4000 PRIMER_PAIR_10_COMPL_ANY=3.00 PRIMER_PAIR_10_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_10=881 PRIMER_PAIR_PENALTY_11=8.6988 PRIMER_LEFT_11_PENALTY=3.597306 PRIMER_RIGHT_11_PENALTY=5.101542 PRIMER_LEFT_11_SEQUENCE=ATGCAGAAATACGAGAAACTGG PRIMER_RIGHT_11_SEQUENCE=TAGGGCGGACAGAAGTCGG PRIMER_LEFT_11=24,22 PRIMER_RIGHT_11=901,19 PRIMER_LEFT_11_TM=58.403 PRIMER_RIGHT_11_TM=63.602 PRIMER_LEFT_11_GC_PERCENT=40.909 PRIMER_RIGHT_11_GC_PERCENT=63.158 PRIMER_LEFT_11_SELF_ANY=4.00 PRIMER_RIGHT_11_SELF_ANY=3.00 PRIMER_LEFT_11_SELF_END=1.00 PRIMER_RIGHT_11_SELF_END=2.00 PRIMER_LEFT_11_POSITION_PENALTY=0.000000 PRIMER_RIGHT_11_POSITION_PENALTY=0.500000 PRIMER_LEFT_11_END_STABILITY=7.9000 PRIMER_RIGHT_11_END_STABILITY=9.6000 PRIMER_PAIR_11_COMPL_ANY=3.00 PRIMER_PAIR_11_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_11=878 PRIMER_PAIR_PENALTY_12=8.9724 PRIMER_LEFT_12_PENALTY=3.597306 PRIMER_RIGHT_12_PENALTY=5.375058 PRIMER_LEFT_12_SEQUENCE=ATGCAGAAATACGAGAAACTGG PRIMER_RIGHT_12_SEQUENCE=CTAGGGCGGACAGAAGTCGG PRIMER_LEFT_12=24,22 PRIMER_RIGHT_12=902,20 PRIMER_LEFT_12_TM=58.403 PRIMER_RIGHT_12_TM=64.375 PRIMER_LEFT_12_GC_PERCENT=40.909 PRIMER_RIGHT_12_GC_PERCENT=65.000 PRIMER_LEFT_12_SELF_ANY=4.00 PRIMER_RIGHT_12_SELF_ANY=4.00 PRIMER_LEFT_12_SELF_END=1.00 PRIMER_RIGHT_12_SELF_END=2.00 PRIMER_LEFT_12_POSITION_PENALTY=0.000000 PRIMER_RIGHT_12_POSITION_PENALTY=1.000000 PRIMER_LEFT_12_END_STABILITY=7.9000 PRIMER_RIGHT_12_END_STABILITY=9.6000 PRIMER_PAIR_12_COMPL_ANY=3.00 PRIMER_PAIR_12_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_12=879 PRIMER_PAIR_PENALTY_13=9.0177 PRIMER_LEFT_13_PENALTY=3.142611 PRIMER_RIGHT_13_PENALTY=5.875058 PRIMER_LEFT_13_SEQUENCE=ATGCAGAAATACGAGAAACTGGA PRIMER_RIGHT_13_SEQUENCE=CCTAGGGCGGACAGAAGTCG PRIMER_LEFT_13=24,23 PRIMER_RIGHT_13=903,20 PRIMER_LEFT_13_TM=60.143 PRIMER_RIGHT_13_TM=64.375 PRIMER_LEFT_13_GC_PERCENT=39.130 PRIMER_RIGHT_13_GC_PERCENT=65.000 PRIMER_LEFT_13_SELF_ANY=4.00 PRIMER_RIGHT_13_SELF_ANY=6.00 PRIMER_LEFT_13_SELF_END=0.00 PRIMER_RIGHT_13_SELF_END=2.00 PRIMER_LEFT_13_POSITION_PENALTY=0.000000 PRIMER_RIGHT_13_POSITION_PENALTY=1.500000 PRIMER_LEFT_13_END_STABILITY=8.2000 PRIMER_RIGHT_13_END_STABILITY=8.1000 PRIMER_PAIR_13_COMPL_ANY=3.00 PRIMER_PAIR_13_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_13=880 PRIMER_PAIR_PENALTY_14=9.0657 PRIMER_LEFT_14_PENALTY=3.597306 PRIMER_RIGHT_14_PENALTY=5.468402 PRIMER_LEFT_14_SEQUENCE=ATGCAGAAATACGAGAAACTGG PRIMER_RIGHT_14_SEQUENCE=GCCTAGGGCGGACAGAAGTC PRIMER_LEFT_14=24,22 PRIMER_RIGHT_14=904,20 PRIMER_LEFT_14_TM=58.403 PRIMER_RIGHT_14_TM=63.468 PRIMER_LEFT_14_GC_PERCENT=40.909 PRIMER_RIGHT_14_GC_PERCENT=65.000 PRIMER_LEFT_14_SELF_ANY=4.00 PRIMER_RIGHT_14_SELF_ANY=6.00 PRIMER_LEFT_14_SELF_END=1.00 PRIMER_RIGHT_14_SELF_END=2.00 PRIMER_LEFT_14_POSITION_PENALTY=0.000000 PRIMER_RIGHT_14_POSITION_PENALTY=2.000000 PRIMER_LEFT_14_END_STABILITY=7.9000 PRIMER_RIGHT_14_END_STABILITY=6.4000 PRIMER_PAIR_14_COMPL_ANY=3.00 PRIMER_PAIR_14_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_14=881 PRIMER_PAIR_PENALTY_15=9.4724 PRIMER_LEFT_15_PENALTY=3.597306 PRIMER_RIGHT_15_PENALTY=5.875058 PRIMER_LEFT_15_SEQUENCE=ATGCAGAAATACGAGAAACTGG PRIMER_RIGHT_15_SEQUENCE=CCTAGGGCGGACAGAAGTCG PRIMER_LEFT_15=24,22 PRIMER_RIGHT_15=903,20 PRIMER_LEFT_15_TM=58.403 PRIMER_RIGHT_15_TM=64.375 PRIMER_LEFT_15_GC_PERCENT=40.909 PRIMER_RIGHT_15_GC_PERCENT=65.000 PRIMER_LEFT_15_SELF_ANY=4.00 PRIMER_RIGHT_15_SELF_ANY=6.00 PRIMER_LEFT_15_SELF_END=1.00 PRIMER_RIGHT_15_SELF_END=2.00 PRIMER_LEFT_15_POSITION_PENALTY=0.000000 PRIMER_RIGHT_15_POSITION_PENALTY=1.500000 PRIMER_LEFT_15_END_STABILITY=7.9000 PRIMER_RIGHT_15_END_STABILITY=8.1000 PRIMER_PAIR_15_COMPL_ANY=3.00 PRIMER_PAIR_15_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_15=880 PRIMER_PAIR_PENALTY_16=10.0066 PRIMER_LEFT_16_PENALTY=5.302203 PRIMER_RIGHT_16_PENALTY=4.704404 PRIMER_LEFT_16_SEQUENCE=ATGCAGAAATACGAGAAACTGGAA PRIMER_RIGHT_16_SEQUENCE=GCCTAGGGCGGACAGAAGT PRIMER_LEFT_16=24,24 PRIMER_RIGHT_16=904,19 PRIMER_LEFT_16_TM=61.302 PRIMER_RIGHT_16_TM=61.704 PRIMER_LEFT_16_GC_PERCENT=37.500 PRIMER_RIGHT_16_GC_PERCENT=63.158 PRIMER_LEFT_16_SELF_ANY=4.00 PRIMER_RIGHT_16_SELF_ANY=6.00 PRIMER_LEFT_16_SELF_END=1.00 PRIMER_RIGHT_16_SELF_END=1.00 PRIMER_LEFT_16_POSITION_PENALTY=0.000000 PRIMER_RIGHT_16_POSITION_PENALTY=2.000000 PRIMER_LEFT_16_END_STABILITY=8.5000 PRIMER_RIGHT_16_END_STABILITY=6.4000 PRIMER_PAIR_16_COMPL_ANY=3.00 PRIMER_PAIR_16_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_16=881 PRIMER_PAIR_PENALTY_17=10.0443 PRIMER_LEFT_17_PENALTY=7.356069 PRIMER_RIGHT_17_PENALTY=2.688260 PRIMER_LEFT_17_SEQUENCE=ATGCAGAAATACGAGAAACTGGAAA PRIMER_RIGHT_17_SEQUENCE=CCTAGGGCGGACAGAAGTC PRIMER_LEFT_17=24,25 PRIMER_RIGHT_17=903,19 PRIMER_LEFT_17_TM=62.356 PRIMER_RIGHT_17_TM=59.812 PRIMER_LEFT_17_GC_PERCENT=36.000 PRIMER_RIGHT_17_GC_PERCENT=63.158 PRIMER_LEFT_17_SELF_ANY=4.00 PRIMER_RIGHT_17_SELF_ANY=6.00 PRIMER_LEFT_17_SELF_END=0.00 PRIMER_RIGHT_17_SELF_END=2.00 PRIMER_LEFT_17_POSITION_PENALTY=0.000000 PRIMER_RIGHT_17_POSITION_PENALTY=1.500000 PRIMER_LEFT_17_END_STABILITY=8.5000 PRIMER_RIGHT_17_END_STABILITY=6.4000 PRIMER_PAIR_17_COMPL_ANY=3.00 PRIMER_PAIR_17_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_17=880 PRIMER_PAIR_PENALTY_18=10.2945 PRIMER_LEFT_18_PENALTY=7.356069 PRIMER_RIGHT_18_PENALTY=2.938464 PRIMER_LEFT_18_SEQUENCE=ATGCAGAAATACGAGAAACTGGAAA PRIMER_RIGHT_18_SEQUENCE=CTAGGGCGGACAGAAGTCG PRIMER_LEFT_18=24,25 PRIMER_RIGHT_18=902,19 PRIMER_LEFT_18_TM=62.356 PRIMER_RIGHT_18_TM=60.938 PRIMER_LEFT_18_GC_PERCENT=36.000 PRIMER_RIGHT_18_GC_PERCENT=63.158 PRIMER_LEFT_18_SELF_ANY=4.00 PRIMER_RIGHT_18_SELF_ANY=4.00 PRIMER_LEFT_18_SELF_END=0.00 PRIMER_RIGHT_18_SELF_END=2.00 PRIMER_LEFT_18_POSITION_PENALTY=0.000000 PRIMER_RIGHT_18_POSITION_PENALTY=1.000000 PRIMER_LEFT_18_END_STABILITY=8.5000 PRIMER_RIGHT_18_END_STABILITY=8.1000 PRIMER_PAIR_18_COMPL_ANY=3.00 PRIMER_PAIR_18_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_18=879 PRIMER_PAIR_PENALTY_19=10.4037 PRIMER_LEFT_19_PENALTY=5.302203 PRIMER_RIGHT_19_PENALTY=5.101542 PRIMER_LEFT_19_SEQUENCE=ATGCAGAAATACGAGAAACTGGAA PRIMER_RIGHT_19_SEQUENCE=TAGGGCGGACAGAAGTCGG PRIMER_LEFT_19=24,24 PRIMER_RIGHT_19=901,19 PRIMER_LEFT_19_TM=61.302 PRIMER_RIGHT_19_TM=63.602 PRIMER_LEFT_19_GC_PERCENT=37.500 PRIMER_RIGHT_19_GC_PERCENT=63.158 PRIMER_LEFT_19_SELF_ANY=4.00 PRIMER_RIGHT_19_SELF_ANY=3.00 PRIMER_LEFT_19_SELF_END=1.00 PRIMER_RIGHT_19_SELF_END=2.00 PRIMER_LEFT_19_POSITION_PENALTY=0.000000 PRIMER_RIGHT_19_POSITION_PENALTY=0.500000 PRIMER_LEFT_19_END_STABILITY=8.5000 PRIMER_RIGHT_19_END_STABILITY=9.6000 PRIMER_PAIR_19_COMPL_ANY=3.00 PRIMER_PAIR_19_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_19=878 = PRIMER_SEQUENCE_ID=L34673/HIP116 PRIMER_COMMENT=Human ATPase, DNA-binding protein (HIP116) mRNA, 3' end. CDS=178..3207 PRIMER_START_CODON_POSITION=177 SEQUENCE=ATTCCCGGGGTCTGACTGGACTCGCGGCGACTTACCTTTCAGTCGTGCGCTCCTGATCCGGCGCTCGGAATTTGTCCCCGGCTTCAGGGCTGCGGGGCCTGGAAGGAGGCGTATCGAGGCGGCTCGAAAACGATCCAGGGGAGCCGAGGCGCTCCTCTTGTCATCCCACTCAGCGCCATGTCCTGGATGTTCAAGAGGGATCCAGTTTGGAAGTACTTGCAGACTGTCCAGTATGGAGTTCATGGAAATTTTCCACGCCTCTCATATCCAACTTTCTTTCCACGTTTTGAATTCCAAGATGTTATCCCTCCAGATGACTTTCTAACTAGTGATGAAGAAGTAGATTCCGTTTTATTTGGAAGTTTGAGAGGTCATGTGGTTGGACTACGCTATTACACGGGAGTAGTTAATAATAATGAAATGGTTGCATTACAACGAGATCCTAATAACCCTTATGATAAGAATGCAATTAAAGTAAACAATGTGAATGGAAATCAAGTTGGCCATTTAAAGAAAGAGCTTGCAGGTGCTTTGGCCTATATCATGGACAACAAATTGGCACAAATTGAAGGGGTAGTTCCTTTTGGTGCAAACAATGCTTTTACCATGCCTCTGCATATGACTTTTTGGGGAAAAGAAGAAAATAGAAAAGCGGTTTCAGATCAGTTGAAGAAACATGGATTTAAATTGGGTCCTGCACCAAAAACTTTAGGATTCAATTTGGAAAGTGGTTGGGGCTCTGGAAGAGCTGGACCAAGCTATAGTATGCCAGTGCATGCTGCAGTACAGATGACAACTGAACAGCTTAAAACAGAATTTGACAAATTGTTTGAAGATTTAAAAGAAGATGATAAAACCCATGAAATGGAACCAGCTGAGGCTATTGAAACACCACTGCTTCCACATCAAAAACAAGCTCTAGCTTGGATGGTGTCACGGGAAAATAGCAAAGAACTTCCACCATTCTGGGAACAGCGAAATGACTTATACTATAACACAATAACAAATTTTTCTGAGAAGGACCGACCAGAAAATGTCCATGGAGGAATTTTAGCTGATGATATGGGTTTGGGTAAAACTCTTACAGCCATTGCAGTAATCCTTACCAACTTCCATGATGGCAGACCTCTTCCTATTGAAAGAGTTAAAAAGAATCTACTGAAGAAGGAATATAATGTTAACGATGACTCTATGAAACTTGGAGGAAACAATACCAGTGAAAAGGCAGATGGACTAAGCAAAGACGCATCTAGATGTAGTGAACAACCCAGTATTTCAGATATCAAGGAGAAGAGTAAGTTTCGCATGTCAGAATTGTCTACGTCCCGCCCCAAAAGAAGAAAAACTGCTGTCCAGTACATAGAAAGCAGTGATTCAGAGGAAATTGAAACAAGTGAATTGCCGCAGAAAATGAAAGGCAAACTGAAAAATGTACAGTCTGAAACTAAAGGCAGGGCGAAAGCAGGATCTTCTAAGGTTATAGAAGATGTGGCATTTGCATGTGCATTAACTTCATCTGTTCCTACAACAAAAAAGAAAATGTTGAAAAAGGGAGCTTGTGCAGTGGAGGGGTCAAAGAAAACTGATGTTGAGGAGAGACCAAGAACAACACTGATCATCTGTCCGCTTTCTGTGTTAAGCAACTGGATTGACCAGTTTGGACAACATATAAAATCAGATGTACACTTGAATTTTTATGTTTATTATGGTCCTGATCGTATTAGAGAACCGGCCTTACTTTCAAAACAGGATATTGTTTTGACTACGTATAATATTTTAACTCATGACTATGGAACTAAAGGAGATAGTCCATTACATAGCATAAGGTGGCTAAGAGTGATCCTGGATGAAGGACATGCCATACGAAATCCAAATGCTCAGCAGACAAAAGCTGTACTTGACTTAGAATCAGAAAGAAGATGGGTTTTGACAGGTACTCCAATCCAGAATTCTTTAAAGGACTTGTGGTCTCTTCTTTCCTTTTTAAAACTTAAACCATTTATTGATAGAGAATGGTGGCATAGAACAATACAGCGTCCTGTCACAATGGGAGATGAAGGAGGACTTAGGCGTTTACAGTCCCTAATTAAAAATATTACACTTAGAAGAACAAAGACAAGCAAAATTAAAGGAAAACCTGTTTTGGAGTTACCAGAACGTAAAGTATTTATTCAGCACATTACACTTTCAGATGAAGAGAGAAAGATTTATCAGTCTGTGAAAAATGAAGGCAGAGCCACTATTGGAAGGTATTTTAATGAAGGGACTGTCCTGGCACATTATGCAGATGTCCTGGGTCTTTTGCTTAGACTGCGGCAAATTTGTTGCCATACTTACCTTCTTACAAATGCAGTGTCTTCCAATGGCCCCTCAGGAAATGATACACCTGAAGAACTGAGAAAGAAGTTAATAAGGAAGATGAAGTTAATTCTGAGCTCAGGTTCAGATGAGGAATGTGCAATTTGCCTGGATTCTTTAACAGTTCCTGTGATAACACATTGTGCACATGTATTTTGTAAACCCTGTATTTGCCAAGTCATTCAGAATGAGCAGCCACATGCTAAATGCCCTTTATGCAGAAATGATATACATGAAGATAATTTATTAGAATGTCCTCCAGAAGAATTAGCACGTGACAGTGAGAAAAAGTCTGATATGGAATGGACATCCAGTTCAAAGATTAATGCGCTAATGCACGCATTGACTGACTTAAGAAAGAAGAATCCCAACATAAAAAGTTTGGTTGTTTCTCAGTTTACAACATTCCTGTCTTTAATAGAAATACCACTTAAAGCCTCTGGATTTGTGTTTACTCGTTTGGATGGTTCCATGGCCCAAAAGAAAAGAGTTGAATCAATTCAGTGTTTTCAAAACACTGAAGCAGGATCTCCAACTATAATGCTTCTGTCCTTAAAAGCAGGTGGAGTTGGTTTGAATCTGTCTGCAGCTTCTCGAGTGTTTTTAATGGATCCAGCCTGGAATCCTGCTGCTGAAGATCAGTGCTTTGACAGATGCCATAGACTTGGTCAGAAGCAAGAAGTTATCATCACAAAATTCATTGTAAAGGACTCTGTTGAAGAAAATATGCTGAAAATACAAAACAAAAAGAGAGAACTTGCAGCAGGAGCCTTTGGAACTAAAAAACCAAATGCTGACGAAATGAAACAAGCCAAAATTAATGAAATCAGAACATTAATTGACTTATAATTTGTGGGATTTTAGTAAGAAGACTACTATATGTGAGAGGCGTGATATCTGGATGGAAGTTGGGCTGGATGATCTCCAAAGTCGTTTCAACTCTTAAAGACATCTTAATCCTGAATGTAAACAATTGTTATGTGTTTAGAATCAGAATTTGATTTTGAACTTGAGTAATTCATCCTTACAGCTATCTGTAGAATTAGTCATCTTTTTTCTT PRIMER_LEFT_EXPLAIN=considered 29448, would not amplify any of the ORF 20059, GC content failed 202, low tm 3593, high tm 1850, long poly-x seq 41, high 3' stability 65, ok 3638 PRIMER_RIGHT_EXPLAIN=considered 29262, GC content failed 608, low tm 11948, high tm 5429, high end compl 9, long poly-x seq 56, high 3' stability 79, ok 11133 PRIMER_PAIR_EXPLAIN=considered 21, ok 21 PRIMER_STOP_CODON_POSITION=3204 PRIMER_PAIR_PENALTY=15.9507 PRIMER_LEFT_PENALTY=1.996678 PRIMER_RIGHT_PENALTY=13.954041 PRIMER_LEFT_SEQUENCE=ATGTCCTGGATGTTCAAGAGG PRIMER_RIGHT_SEQUENCE=CCCACAAATTATAAGTCAATTAATGTT PRIMER_LEFT=177,21 PRIMER_RIGHT=3214,27 PRIMER_LEFT_TM=59.003 PRIMER_RIGHT_TM=58.046 PRIMER_LEFT_GC_PERCENT=47.619 PRIMER_RIGHT_GC_PERCENT=25.926 PRIMER_LEFT_SELF_ANY=5.00 PRIMER_RIGHT_SELF_ANY=6.00 PRIMER_LEFT_SELF_END=0.00 PRIMER_RIGHT_SELF_END=3.00 PRIMER_LEFT_POSITION_PENALTY=0.000000 PRIMER_RIGHT_POSITION_PENALTY=5.000000 PRIMER_LEFT_END_STABILITY=7.9000 PRIMER_RIGHT_END_STABILITY=6.6000 PRIMER_PAIR_COMPL_ANY=3.00 PRIMER_PAIR_COMPL_END=0.00 PRIMER_PRODUCT_SIZE=3038 PRIMER_PAIR_PENALTY_1=18.4775 PRIMER_LEFT_1_PENALTY=4.523430 PRIMER_RIGHT_1_PENALTY=13.954041 PRIMER_LEFT_1_SEQUENCE=ATGTCCTGGATGTTCAAGAGGG PRIMER_RIGHT_1_SEQUENCE=CCCACAAATTATAAGTCAATTAATGTT PRIMER_LEFT_1=177,22 PRIMER_RIGHT_1=3214,27 PRIMER_LEFT_1_TM=62.523 PRIMER_RIGHT_1_TM=58.046 PRIMER_LEFT_1_GC_PERCENT=50.000 PRIMER_RIGHT_1_GC_PERCENT=25.926 PRIMER_LEFT_1_SELF_ANY=5.00 PRIMER_RIGHT_1_SELF_ANY=6.00 PRIMER_LEFT_1_SELF_END=0.00 PRIMER_RIGHT_1_SELF_END=3.00 PRIMER_LEFT_1_POSITION_PENALTY=0.000000 PRIMER_RIGHT_1_POSITION_PENALTY=5.000000 PRIMER_LEFT_1_END_STABILITY=9.4000 PRIMER_RIGHT_1_END_STABILITY=6.6000 PRIMER_PAIR_1_COMPL_ANY=3.00 PRIMER_PAIR_1_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_1=3038 PRIMER_PAIR_PENALTY_2=19.3757 PRIMER_LEFT_2_PENALTY=1.996678 PRIMER_RIGHT_2_PENALTY=17.378993 PRIMER_LEFT_2_SEQUENCE=ATGTCCTGGATGTTCAAGAGG PRIMER_RIGHT_2_SEQUENCE=CTAAAATCCCACAAATTATAAGTCAA PRIMER_LEFT_2=177,21 PRIMER_RIGHT_2=3221,26 PRIMER_LEFT_2_TM=59.003 PRIMER_RIGHT_2_TM=57.121 PRIMER_LEFT_2_GC_PERCENT=47.619 PRIMER_RIGHT_2_GC_PERCENT=26.923 PRIMER_LEFT_2_SELF_ANY=5.00 PRIMER_RIGHT_2_SELF_ANY=6.00 PRIMER_LEFT_2_SELF_END=0.00 PRIMER_RIGHT_2_SELF_END=0.00 PRIMER_LEFT_2_POSITION_PENALTY=0.000000 PRIMER_RIGHT_2_POSITION_PENALTY=8.500000 PRIMER_LEFT_2_END_STABILITY=7.9000 PRIMER_RIGHT_2_END_STABILITY=6.7000 PRIMER_PAIR_2_COMPL_ANY=5.00 PRIMER_PAIR_2_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_2=3045 PRIMER_PAIR_PENALTY_3=20.0477 PRIMER_LEFT_3_PENALTY=1.996678 PRIMER_RIGHT_3_PENALTY=18.051002 PRIMER_LEFT_3_SEQUENCE=ATGTCCTGGATGTTCAAGAGG PRIMER_RIGHT_3_SEQUENCE=CTAAAATCCCACAAATTATAAGTCAAT PRIMER_LEFT_3=177,21 PRIMER_RIGHT_3=3221,27 PRIMER_LEFT_3_TM=59.003 PRIMER_RIGHT_3_TM=57.449 PRIMER_LEFT_3_GC_PERCENT=47.619 PRIMER_RIGHT_3_GC_PERCENT=25.926 PRIMER_LEFT_3_SELF_ANY=5.00 PRIMER_RIGHT_3_SELF_ANY=6.00 PRIMER_LEFT_3_SELF_END=0.00 PRIMER_RIGHT_3_SELF_END=2.00 PRIMER_LEFT_3_POSITION_PENALTY=0.000000 PRIMER_RIGHT_3_POSITION_PENALTY=8.500000 PRIMER_LEFT_3_END_STABILITY=7.9000 PRIMER_RIGHT_3_END_STABILITY=6.9000 PRIMER_PAIR_3_COMPL_ANY=5.00 PRIMER_PAIR_3_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_3=3045 PRIMER_PAIR_PENALTY_4=20.1564 PRIMER_LEFT_4_PENALTY=1.996678 PRIMER_RIGHT_4_PENALTY=18.159733 PRIMER_LEFT_4_SEQUENCE=ATGTCCTGGATGTTCAAGAGG PRIMER_RIGHT_4_SEQUENCE=ACTAAAATCCCACAAATTATAAGTCAA PRIMER_LEFT_4=177,21 PRIMER_RIGHT_4=3222,27 PRIMER_LEFT_4_TM=59.003 PRIMER_RIGHT_4_TM=57.840 PRIMER_LEFT_4_GC_PERCENT=47.619 PRIMER_RIGHT_4_GC_PERCENT=25.926 PRIMER_LEFT_4_SELF_ANY=5.00 PRIMER_RIGHT_4_SELF_ANY=6.00 PRIMER_LEFT_4_SELF_END=0.00 PRIMER_RIGHT_4_SELF_END=0.00 PRIMER_LEFT_4_POSITION_PENALTY=0.000000 PRIMER_RIGHT_4_POSITION_PENALTY=9.000000 PRIMER_LEFT_4_END_STABILITY=7.9000 PRIMER_RIGHT_4_END_STABILITY=6.7000 PRIMER_PAIR_4_COMPL_ANY=5.00 PRIMER_PAIR_4_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_4=3046 PRIMER_PAIR_PENALTY_5=21.1472 PRIMER_LEFT_5_PENALTY=7.193142 PRIMER_RIGHT_5_PENALTY=13.954041 PRIMER_LEFT_5_SEQUENCE=ATGTCCTGGATGTTCAAGAGGGA PRIMER_RIGHT_5_SEQUENCE=CCCACAAATTATAAGTCAATTAATGTT PRIMER_LEFT_5=177,23 PRIMER_RIGHT_5=3214,27 PRIMER_LEFT_5_TM=64.193 PRIMER_RIGHT_5_TM=58.046 PRIMER_LEFT_5_GC_PERCENT=47.826 PRIMER_RIGHT_5_GC_PERCENT=25.926 PRIMER_LEFT_5_SELF_ANY=5.00 PRIMER_RIGHT_5_SELF_ANY=6.00 PRIMER_LEFT_5_SELF_END=0.00 PRIMER_RIGHT_5_SELF_END=3.00 PRIMER_LEFT_5_POSITION_PENALTY=0.000000 PRIMER_RIGHT_5_POSITION_PENALTY=5.000000 PRIMER_LEFT_5_END_STABILITY=9.4000 PRIMER_RIGHT_5_END_STABILITY=6.6000 PRIMER_PAIR_5_COMPL_ANY=3.00 PRIMER_PAIR_5_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_5=3038 PRIMER_PAIR_PENALTY_6=21.9024 PRIMER_LEFT_6_PENALTY=4.523430 PRIMER_RIGHT_6_PENALTY=17.378993 PRIMER_LEFT_6_SEQUENCE=ATGTCCTGGATGTTCAAGAGGG PRIMER_RIGHT_6_SEQUENCE=CTAAAATCCCACAAATTATAAGTCAA PRIMER_LEFT_6=177,22 PRIMER_RIGHT_6=3221,26 PRIMER_LEFT_6_TM=62.523 PRIMER_RIGHT_6_TM=57.121 PRIMER_LEFT_6_GC_PERCENT=50.000 PRIMER_RIGHT_6_GC_PERCENT=26.923 PRIMER_LEFT_6_SELF_ANY=5.00 PRIMER_RIGHT_6_SELF_ANY=6.00 PRIMER_LEFT_6_SELF_END=0.00 PRIMER_RIGHT_6_SELF_END=0.00 PRIMER_LEFT_6_POSITION_PENALTY=0.000000 PRIMER_RIGHT_6_POSITION_PENALTY=8.500000 PRIMER_LEFT_6_END_STABILITY=9.4000 PRIMER_RIGHT_6_END_STABILITY=6.7000 PRIMER_PAIR_6_COMPL_ANY=5.00 PRIMER_PAIR_6_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_6=3045 PRIMER_PAIR_PENALTY_7=22.1908 PRIMER_LEFT_7_PENALTY=8.236744 PRIMER_RIGHT_7_PENALTY=13.954041 PRIMER_LEFT_7_SEQUENCE=ATGTCCTGGATGTTCAAGAGGGAT PRIMER_RIGHT_7_SEQUENCE=CCCACAAATTATAAGTCAATTAATGTT PRIMER_LEFT_7=177,24 PRIMER_RIGHT_7=3214,27 PRIMER_LEFT_7_TM=64.237 PRIMER_RIGHT_7_TM=58.046 PRIMER_LEFT_7_GC_PERCENT=45.833 PRIMER_RIGHT_7_GC_PERCENT=25.926 PRIMER_LEFT_7_SELF_ANY=5.00 PRIMER_RIGHT_7_SELF_ANY=6.00 PRIMER_LEFT_7_SELF_END=2.00 PRIMER_RIGHT_7_SELF_END=3.00 PRIMER_LEFT_7_POSITION_PENALTY=0.000000 PRIMER_RIGHT_7_POSITION_PENALTY=5.000000 PRIMER_LEFT_7_END_STABILITY=9.3000 PRIMER_RIGHT_7_END_STABILITY=6.6000 PRIMER_PAIR_7_COMPL_ANY=3.00 PRIMER_PAIR_7_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_7=3038 PRIMER_PAIR_PENALTY_8=22.5744 PRIMER_LEFT_8_PENALTY=4.523430 PRIMER_RIGHT_8_PENALTY=18.051002 PRIMER_LEFT_8_SEQUENCE=ATGTCCTGGATGTTCAAGAGGG PRIMER_RIGHT_8_SEQUENCE=CTAAAATCCCACAAATTATAAGTCAAT PRIMER_LEFT_8=177,22 PRIMER_RIGHT_8=3221,27 PRIMER_LEFT_8_TM=62.523 PRIMER_RIGHT_8_TM=57.449 PRIMER_LEFT_8_GC_PERCENT=50.000 PRIMER_RIGHT_8_GC_PERCENT=25.926 PRIMER_LEFT_8_SELF_ANY=5.00 PRIMER_RIGHT_8_SELF_ANY=6.00 PRIMER_LEFT_8_SELF_END=0.00 PRIMER_RIGHT_8_SELF_END=2.00 PRIMER_LEFT_8_POSITION_PENALTY=0.000000 PRIMER_RIGHT_8_POSITION_PENALTY=8.500000 PRIMER_LEFT_8_END_STABILITY=9.4000 PRIMER_RIGHT_8_END_STABILITY=6.9000 PRIMER_PAIR_8_COMPL_ANY=5.00 PRIMER_PAIR_8_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_8=3045 PRIMER_PAIR_PENALTY_9=22.6832 PRIMER_LEFT_9_PENALTY=4.523430 PRIMER_RIGHT_9_PENALTY=18.159733 PRIMER_LEFT_9_SEQUENCE=ATGTCCTGGATGTTCAAGAGGG PRIMER_RIGHT_9_SEQUENCE=ACTAAAATCCCACAAATTATAAGTCAA PRIMER_LEFT_9=177,22 PRIMER_RIGHT_9=3222,27 PRIMER_LEFT_9_TM=62.523 PRIMER_RIGHT_9_TM=57.840 PRIMER_LEFT_9_GC_PERCENT=50.000 PRIMER_RIGHT_9_GC_PERCENT=25.926 PRIMER_LEFT_9_SELF_ANY=5.00 PRIMER_RIGHT_9_SELF_ANY=6.00 PRIMER_LEFT_9_SELF_END=0.00 PRIMER_RIGHT_9_SELF_END=0.00 PRIMER_LEFT_9_POSITION_PENALTY=0.000000 PRIMER_RIGHT_9_POSITION_PENALTY=9.000000 PRIMER_LEFT_9_END_STABILITY=9.4000 PRIMER_RIGHT_9_END_STABILITY=6.7000 PRIMER_PAIR_9_COMPL_ANY=5.00 PRIMER_PAIR_9_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_9=3046 PRIMER_PAIR_PENALTY_10=24.2545 PRIMER_LEFT_10_PENALTY=1.996678 PRIMER_RIGHT_10_PENALTY=22.257817 PRIMER_LEFT_10_SEQUENCE=ATGTCCTGGATGTTCAAGAGG PRIMER_RIGHT_10_SEQUENCE=TCTTCTTACTAAAATCCCACAAATTAT PRIMER_LEFT_10=177,21 PRIMER_RIGHT_10=3229,27 PRIMER_LEFT_10_TM=59.003 PRIMER_RIGHT_10_TM=57.242 PRIMER_LEFT_10_GC_PERCENT=47.619 PRIMER_RIGHT_10_GC_PERCENT=25.926 PRIMER_LEFT_10_SELF_ANY=5.00 PRIMER_RIGHT_10_SELF_ANY=5.00 PRIMER_LEFT_10_SELF_END=0.00 PRIMER_RIGHT_10_SELF_END=3.00 PRIMER_LEFT_10_POSITION_PENALTY=0.000000 PRIMER_RIGHT_10_POSITION_PENALTY=12.500000 PRIMER_LEFT_10_END_STABILITY=7.9000 PRIMER_RIGHT_10_END_STABILITY=5.8000 PRIMER_PAIR_10_COMPL_ANY=5.00 PRIMER_PAIR_10_COMPL_END=3.00 PRIMER_PRODUCT_SIZE_10=3053 PRIMER_PAIR_PENALTY_11=24.2579 PRIMER_LEFT_11_PENALTY=1.996678 PRIMER_RIGHT_11_PENALTY=22.261240 PRIMER_LEFT_11_SEQUENCE=ATGTCCTGGATGTTCAAGAGG PRIMER_RIGHT_11_SEQUENCE=AGTCTTCTTACTAAAATCCCACAAAT PRIMER_LEFT_11=177,21 PRIMER_RIGHT_11=3231,26 PRIMER_LEFT_11_TM=59.003 PRIMER_RIGHT_11_TM=57.239 PRIMER_LEFT_11_GC_PERCENT=47.619 PRIMER_RIGHT_11_GC_PERCENT=30.769 PRIMER_LEFT_11_SELF_ANY=5.00 PRIMER_RIGHT_11_SELF_ANY=5.00 PRIMER_LEFT_11_SELF_END=0.00 PRIMER_RIGHT_11_SELF_END=2.00 PRIMER_LEFT_11_POSITION_PENALTY=0.000000 PRIMER_RIGHT_11_POSITION_PENALTY=13.500000 PRIMER_LEFT_11_END_STABILITY=7.9000 PRIMER_RIGHT_11_END_STABILITY=7.2000 PRIMER_PAIR_11_COMPL_ANY=5.00 PRIMER_PAIR_11_COMPL_END=3.00 PRIMER_PRODUCT_SIZE_11=3055 PRIMER_PAIR_PENALTY_12=24.3639 PRIMER_LEFT_12_PENALTY=1.996678 PRIMER_RIGHT_12_PENALTY=22.367225 PRIMER_LEFT_12_SEQUENCE=ATGTCCTGGATGTTCAAGAGG PRIMER_RIGHT_12_SEQUENCE=GTCTTCTTACTAAAATCCCACAAATTA PRIMER_LEFT_12=177,21 PRIMER_RIGHT_12=3230,27 PRIMER_LEFT_12_TM=59.003 PRIMER_RIGHT_12_TM=57.633 PRIMER_LEFT_12_GC_PERCENT=47.619 PRIMER_RIGHT_12_GC_PERCENT=29.630 PRIMER_LEFT_12_SELF_ANY=5.00 PRIMER_RIGHT_12_SELF_ANY=5.00 PRIMER_LEFT_12_SELF_END=0.00 PRIMER_RIGHT_12_SELF_END=2.00 PRIMER_LEFT_12_POSITION_PENALTY=0.000000 PRIMER_RIGHT_12_POSITION_PENALTY=13.000000 PRIMER_LEFT_12_END_STABILITY=7.9000 PRIMER_RIGHT_12_END_STABILITY=6.2000 PRIMER_PAIR_12_COMPL_ANY=5.00 PRIMER_PAIR_12_COMPL_END=3.00 PRIMER_PRODUCT_SIZE_12=3054 PRIMER_PAIR_PENALTY_13=24.5721 PRIMER_LEFT_13_PENALTY=7.193142 PRIMER_RIGHT_13_PENALTY=17.378993 PRIMER_LEFT_13_SEQUENCE=ATGTCCTGGATGTTCAAGAGGGA PRIMER_RIGHT_13_SEQUENCE=CTAAAATCCCACAAATTATAAGTCAA PRIMER_LEFT_13=177,23 PRIMER_RIGHT_13=3221,26 PRIMER_LEFT_13_TM=64.193 PRIMER_RIGHT_13_TM=57.121 PRIMER_LEFT_13_GC_PERCENT=47.826 PRIMER_RIGHT_13_GC_PERCENT=26.923 PRIMER_LEFT_13_SELF_ANY=5.00 PRIMER_RIGHT_13_SELF_ANY=6.00 PRIMER_LEFT_13_SELF_END=0.00 PRIMER_RIGHT_13_SELF_END=0.00 PRIMER_LEFT_13_POSITION_PENALTY=0.000000 PRIMER_RIGHT_13_POSITION_PENALTY=8.500000 PRIMER_LEFT_13_END_STABILITY=9.4000 PRIMER_RIGHT_13_END_STABILITY=6.7000 PRIMER_PAIR_13_COMPL_ANY=5.00 PRIMER_PAIR_13_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_13=3045 PRIMER_PAIR_PENALTY_14=25.2441 PRIMER_LEFT_14_PENALTY=7.193142 PRIMER_RIGHT_14_PENALTY=18.051002 PRIMER_LEFT_14_SEQUENCE=ATGTCCTGGATGTTCAAGAGGGA PRIMER_RIGHT_14_SEQUENCE=CTAAAATCCCACAAATTATAAGTCAAT PRIMER_LEFT_14=177,23 PRIMER_RIGHT_14=3221,27 PRIMER_LEFT_14_TM=64.193 PRIMER_RIGHT_14_TM=57.449 PRIMER_LEFT_14_GC_PERCENT=47.826 PRIMER_RIGHT_14_GC_PERCENT=25.926 PRIMER_LEFT_14_SELF_ANY=5.00 PRIMER_RIGHT_14_SELF_ANY=6.00 PRIMER_LEFT_14_SELF_END=0.00 PRIMER_RIGHT_14_SELF_END=2.00 PRIMER_LEFT_14_POSITION_PENALTY=0.000000 PRIMER_RIGHT_14_POSITION_PENALTY=8.500000 PRIMER_LEFT_14_END_STABILITY=9.4000 PRIMER_RIGHT_14_END_STABILITY=6.9000 PRIMER_PAIR_14_COMPL_ANY=5.00 PRIMER_PAIR_14_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_14=3045 PRIMER_PAIR_PENALTY_15=25.3529 PRIMER_LEFT_15_PENALTY=7.193142 PRIMER_RIGHT_15_PENALTY=18.159733 PRIMER_LEFT_15_SEQUENCE=ATGTCCTGGATGTTCAAGAGGGA PRIMER_RIGHT_15_SEQUENCE=ACTAAAATCCCACAAATTATAAGTCAA PRIMER_LEFT_15=177,23 PRIMER_RIGHT_15=3222,27 PRIMER_LEFT_15_TM=64.193 PRIMER_RIGHT_15_TM=57.840 PRIMER_LEFT_15_GC_PERCENT=47.826 PRIMER_RIGHT_15_GC_PERCENT=25.926 PRIMER_LEFT_15_SELF_ANY=5.00 PRIMER_RIGHT_15_SELF_ANY=6.00 PRIMER_LEFT_15_SELF_END=0.00 PRIMER_RIGHT_15_SELF_END=0.00 PRIMER_LEFT_15_POSITION_PENALTY=0.000000 PRIMER_RIGHT_15_POSITION_PENALTY=9.000000 PRIMER_LEFT_15_END_STABILITY=9.4000 PRIMER_RIGHT_15_END_STABILITY=6.7000 PRIMER_PAIR_15_COMPL_ANY=5.00 PRIMER_PAIR_15_COMPL_END=0.00 PRIMER_PRODUCT_SIZE_15=3046 PRIMER_PAIR_PENALTY_16=25.6157 PRIMER_LEFT_16_PENALTY=8.236744 PRIMER_RIGHT_16_PENALTY=17.378993 PRIMER_LEFT_16_SEQUENCE=ATGTCCTGGATGTTCAAGAGGGAT PRIMER_RIGHT_16_SEQUENCE=CTAAAATCCCACAAATTATAAGTCAA PRIMER_LEFT_16=177,24 PRIMER_RIGHT_16=3221,26 PRIMER_LEFT_16_TM=64.237 PRIMER_RIGHT_16_TM=57.121 PRIMER_LEFT_16_GC_PERCENT=45.833 PRIMER_RIGHT_16_GC_PERCENT=26.923 PRIMER_LEFT_16_SELF_ANY=5.00 PRIMER_RIGHT_16_SELF_ANY=6.00 PRIMER_LEFT_16_SELF_END=2.00 PRIMER_RIGHT_16_SELF_END=0.00 PRIMER_LEFT_16_POSITION_PENALTY=0.000000 PRIMER_RIGHT_16_POSITION_PENALTY=8.500000 PRIMER_LEFT_16_END_STABILITY=9.3000 PRIMER_RIGHT_16_END_STABILITY=6.7000 PRIMER_PAIR_16_COMPL_ANY=5.00 PRIMER_PAIR_16_COMPL_END=1.00 PRIMER_PRODUCT_SIZE_16=3045 PRIMER_PAIR_PENALTY_17=25.6674 PRIMER_LEFT_17_PENALTY=1.996678 PRIMER_RIGHT_17_PENALTY=23.670687 PRIMER_LEFT_17_SEQUENCE=ATGTCCTGGATGTTCAAGAGG PRIMER_RIGHT_17_SEQUENCE=TAGTCTTCTTACTAAAATCCCACAAAT PRIMER_LEFT_17=177,21 PRIMER_RIGHT_17=3232,27 PRIMER_LEFT_17_TM=59.003 PRIMER_RIGHT_17_TM=57.329 PRIMER_LEFT_17_GC_PERCENT=47.619 PRIMER_RIGHT_17_GC_PERCENT=29.630 PRIMER_LEFT_17_SELF_ANY=5.00 PRIMER_RIGHT_17_SELF_ANY=5.00 PRIMER_LEFT_17_SELF_END=0.00 PRIMER_RIGHT_17_SELF_END=2.00 PRIMER_LEFT_17_POSITION_PENALTY=0.000000 PRIMER_RIGHT_17_POSITION_PENALTY=14.000000 PRIMER_LEFT_17_END_STABILITY=7.9000 PRIMER_RIGHT_17_END_STABILITY=7.2000 PRIMER_PAIR_17_COMPL_ANY=5.00 PRIMER_PAIR_17_COMPL_END=3.00 PRIMER_PRODUCT_SIZE_17=3056 PRIMER_PAIR_PENALTY_18=25.7689 PRIMER_LEFT_18_PENALTY=1.996678 PRIMER_RIGHT_18_PENALTY=23.772253 PRIMER_LEFT_18_SEQUENCE=ATGTCCTGGATGTTCAAGAGG PRIMER_RIGHT_18_SEQUENCE=GTAGTCTTCTTACTAAAATCCCACAAA PRIMER_LEFT_18=177,21 PRIMER_RIGHT_18=3233,27 PRIMER_LEFT_18_TM=59.003 PRIMER_RIGHT_18_TM=57.728 PRIMER_LEFT_18_GC_PERCENT=47.619 PRIMER_RIGHT_18_GC_PERCENT=33.333 PRIMER_LEFT_18_SELF_ANY=5.00 PRIMER_RIGHT_18_SELF_ANY=5.00 PRIMER_LEFT_18_SELF_END=0.00 PRIMER_RIGHT_18_SELF_END=0.00 PRIMER_LEFT_18_POSITION_PENALTY=0.000000 PRIMER_RIGHT_18_POSITION_PENALTY=14.500000 PRIMER_LEFT_18_END_STABILITY=7.9000 PRIMER_RIGHT_18_END_STABILITY=7.0000 PRIMER_PAIR_18_COMPL_ANY=5.00 PRIMER_PAIR_18_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_18=3057 PRIMER_PAIR_PENALTY_19=26.2877 PRIMER_LEFT_19_PENALTY=8.236744 PRIMER_RIGHT_19_PENALTY=18.051002 PRIMER_LEFT_19_SEQUENCE=ATGTCCTGGATGTTCAAGAGGGAT PRIMER_RIGHT_19_SEQUENCE=CTAAAATCCCACAAATTATAAGTCAAT PRIMER_LEFT_19=177,24 PRIMER_RIGHT_19=3221,27 PRIMER_LEFT_19_TM=64.237 PRIMER_RIGHT_19_TM=57.449 PRIMER_LEFT_19_GC_PERCENT=45.833 PRIMER_RIGHT_19_GC_PERCENT=25.926 PRIMER_LEFT_19_SELF_ANY=5.00 PRIMER_RIGHT_19_SELF_ANY=6.00 PRIMER_LEFT_19_SELF_END=2.00 PRIMER_RIGHT_19_SELF_END=2.00 PRIMER_LEFT_19_POSITION_PENALTY=0.000000 PRIMER_RIGHT_19_POSITION_PENALTY=8.500000 PRIMER_LEFT_19_END_STABILITY=9.3000 PRIMER_RIGHT_19_END_STABILITY=6.9000 PRIMER_PAIR_19_COMPL_ANY=5.00 PRIMER_PAIR_19_COMPL_END=2.00 PRIMER_PRODUCT_SIZE_19=3045 =