ACCEPTABLE RIGHT PRIMERS 1-based # self self qual- # sequence start ln N GC% Tm any end lity 0 TCCTGTTGGAGAAAAGCCAC 248 20 0 50.00 60.232 3.00 0.00 0.232 1 CCTGTTGGAGAAAAGCCACT 247 20 0 50.00 59.328 3.00 1.00 0.672 2 TTCCTGTTGGAGAAAAGCCA 249 20 0 45.00 60.745 3.00 0.00 0.745 3 TGTTGGAGAAAAGCCACTGG 245 20 0 50.00 61.204 5.00 5.00 1.204 4 CTGTTGGAGAAAAGCCACTG 246 20 0 50.00 58.496 3.00 3.00 1.504 5 TCCTGTTGGAGAAAAGCCA 248 19 0 47.37 59.354 3.00 0.00 1.646 6 TGGAGAAAAGCCACTGGC 242 18 0 55.56 59.918 7.00 7.00 2.082 7 GGAGAAAAGCCACTGGCA 241 18 0 55.56 59.918 7.00 5.00 2.082 8 GTTGGAGAAAAGCCACTGGC 244 20 0 55.00 62.088 7.00 7.00 2.088 9 TCCTGTTGGAGAAAAGCCACT 248 21 0 47.62 61.165 3.00 1.00 2.165 10 TTGGAGAAAAGCCACTGGC 243 19 0 52.63 61.313 7.00 7.00 2.313 11 TTCCTGTTGGAGAAAAGCCAC 249 21 0 47.62 61.523 3.00 0.00 2.523 12 CCTGTTGGAGAAAAGCCAC 247 19 0 52.63 58.272 3.00 0.00 2.728 13 GTTGGAGAAAAGCCACTGG 244 19 0 52.63 58.272 5.00 5.00 2.728 14 CCTGTTGGAGAAAAGCCACTG 247 21 0 52.38 62.093 3.00 3.00 3.093 15 CTGTTGGAGAAAAGCCACTGG 246 21 0 52.38 62.093 5.00 5.00 3.093 16 TTCCTGTTGGAGAAAAGCC 249 19 0 47.37 57.850 3.00 1.00 3.150 17 TGTTGGAGAAAAGCCACTG 245 19 0 47.37 57.360 3.00 3.00 3.640 18 TGGAGAAAAGCCACTGGCA 242 19 0 52.63 62.902 7.00 5.00 3.902 19 TTCCTGTTGGAGAAAAGCCACT 249 22 0 45.45 62.351 3.00 1.00 4.351 20 CCTGTTGGAGAAAAGCCA 247 18 0 50.00 57.278 3.00 0.00 4.722 21 TTGGAGAAAAGCCACTGG 243 18 0 50.00 57.278 5.00 5.00 4.722 22 GACCCCACGCACGCTAAG 159 18 0 66.67 62.798 3.00 3.00 4.798