ACCEPTABLE LEFT PRIMERS 1-based # self self qual- # sequence start ln N GC% Tm any end lity 0 TGAATCGCAATCAAATTTTTTC 33 22 0 27.27 59.091 6.00 2.00 2.909 1 TGAATCGCAATCAAATTTTTTCT 33 23 0 26.09 59.967 6.00 2.00 3.033 2 ATGAATCGCAATCAAATTTTTTC 32 23 0 26.09 59.374 6.00 2.00 3.626 3 TGAATCGCAATCAAATTTTTT 33 21 0 23.81 57.334 6.00 4.00 3.666 4 TGAATCGCAATCAAATTTTTTCTA 33 24 0 25.00 59.977 6.00 2.00 4.023 5 ATGAATCGCAATCAAATTTTTTCT 32 24 0 25.00 60.199 6.00 2.00 4.199 6 TCGCAATCAAATTTTTTCTATGA 37 23 0 26.09 58.793 6.00 2.00 4.207 7 ATGAATCGCAATCAAATTTTTT 32 22 0 22.73 57.710 6.00 4.00 4.290 8 TCGCAATCAAATTTTTTCTATGAC 37 24 0 29.17 59.542 6.00 1.00 4.458 9 GAATCGCAATCAAATTTTTTCT 34 22 0 27.27 57.467 6.00 2.00 4.533 10 TATGAATCGCAATCAAATTTTTTC 31 24 0 25.00 59.404 6.00 2.00 4.596 11 ATCGCAATCAAATTTTTTCTATGA 36 24 0 25.00 59.083 6.00 3.00 4.917 12 TCGCAATCAAATTTTTTCTATG 37 22 0 27.27 57.077 6.00 2.00 4.923 13 TTATGAATCGCAATCAAATTTTTT 30 24 0 20.83 58.994 6.00 4.00 5.006 14 AATCGCAATCAAATTTTTTCTATGA 35 25 0 24.00 60.202 6.00 2.00 5.202 15 GAATCGCAATCAAATTTTTTCTATG 34 25 0 28.00 60.202 6.00 2.00 5.202 16 TGAATCGCAATCAAATTTTTTCTAT 33 25 0 24.00 60.202 6.00 4.00 5.202 17 ATGAATCGCAATCAAATTTTTTCTA 32 25 0 24.00 60.202 6.00 2.00 5.202 18 TATGAATCGCAATCAAATTTTTTCT 31 25 0 24.00 60.202 6.00 2.00 5.202 19 TATGAATCGCAATCAAATTTTTT 31 23 0 21.74 57.795 6.00 4.00 5.205 20 TTATGAATCGCAATCAAATTTTT 30 23 0 21.74 57.795 6.00 4.00 5.205 21 ATCGCAATCAAATTTTTTCTATGAC 36 25 0 28.00 59.789 6.00 2.00 5.211 22 AATCGCAATCAAATTTTTTCTATG 35 24 0 25.00 58.677 6.00 2.00 5.323 23 GAATCGCAATCAAATTTTTTCTA 34 23 0 26.09 57.561 6.00 2.00 5.439 24 TTATGAATCGCAATCAAATTTTTTC 30 25 0 24.00 60.502 6.00 2.00 5.502 25 ATCGCAATCAAATTTTTTCTATG 36 23 0 26.09 57.456 6.00 2.00 5.544 26 ATTATGAATCGCAATCAAATTTTTT 29 25 0 20.00 59.255 6.00 4.00 5.745 27 AATTATGAATCGCAATCAAATTTTT 28 25 0 20.00 59.255 8.00 4.00 5.745 28 ATTATGAATCGCAATCAAATTTTT 29 24 0 20.83 58.122 6.00 4.00 5.878 29 AATTATGAATCGCAATCAAATTTT 28 24 0 20.83 58.122 8.00 5.00 5.878 30 GAATCGCAATCAAATTTTTTCTAT 34 24 0 25.00 57.900 6.00 4.00 6.100 31 TATGAATCGCAATCAAATTTTTTCTA 31 26 0 23.08 60.204 6.00 2.00 6.204 32 ATGAATCGCAATCAAATTTTTTCTAT 32 26 0 23.08 60.408 6.00 4.00 6.408 33 ATTATGAATCGCAATCAAATTTTTTC 29 26 0 23.08 60.694 6.00 2.00 6.694 34 AATCGCAATCAAATTTTTTCTATGAC 35 26 0 26.92 60.843 6.00 1.00 6.843 35 TTATGAATCGCAATCAAATTTTTTCT 30 26 0 23.08 61.226 6.00 2.00 7.226 36 TATGAATCGCAATCAAATTTTTTCTAT 31 27 0 22.22 60.404 6.00 4.00 7.404 37 TCGCAATCAAATTTTTTCTATGACC 37 25 0 32.00 62.463 6.00 0.00 7.463 38 GAATCGCAATCAAATTTTTTCTATGA 34 26 0 26.92 61.653 6.00 2.00 7.653 39 TTATGAATCGCAATCAAATTTTTTCTA 30 27 0 22.22 61.199 6.00 2.00 8.199 40 ATTATGAATCGCAATCAAATTTTTTCT 29 27 0 22.22 61.382 6.00 2.00 8.382 41 TGAATCGCAATCAAATTTTTTCTATG 33 26 0 26.92 62.397 6.00 2.00 8.397 42 ATCGCAATCAAATTTTTTCTATGACC 36 26 0 30.77 62.574 6.00 0.00 8.574 43 AATTATGAATCGCAATCAAATTTTTTC 28 27 0 22.22 61.652 8.00 2.00 8.652 44 GAATCGCAATCAAATTTTTTCTATGAC 34 27 0 29.63 62.235 6.00 1.00 9.235 45 ATGAATCGCAATCAAATTTTTTCTATG 32 27 0 25.93 62.507 6.00 2.00 9.507