ACCEPTABLE LEFT PRIMERS 1-based # self self qual- # sequence start ln N GC% Tm any end lity 0 TTAGAAGGGGTGTCTGGGTG 73 20 0 55.00 59.959 3.00 0.00 0.041 1 CTGCAGGTTTAGAAGGGGTG 65 20 0 55.00 59.728 6.00 0.00 0.272 2 CACCCTGCAGGTTTAGAAGG 61 20 0 55.00 59.728 8.00 0.00 0.272 3 AGCACACCCTGCAGGTTTAG 57 20 0 55.00 60.312 8.00 6.00 0.312 4 TGCAGGTTTAGAAGGGGTGT 66 20 0 50.00 59.592 4.00 0.00 0.408 5 CCTGCAGGTTTAGAAGGGGT 64 20 0 55.00 60.488 8.00 0.00 0.488 6 ACCCTGCAGGTTTAGAAGGG 62 20 0 55.00 60.488 8.00 2.00 0.488 7 CACCCTGTAAACCCTGGCT 8 19 0 57.89 59.974 3.00 3.00 1.026 8 TCTGCACCCTGTAAACCCTG 4 20 0 55.00 61.084 4.00 1.00 1.084 9 CACACCCTGCAGGTTTAGAAG 59 21 0 52.38 59.782 8.00 2.00 1.218 10 CACACCCTGCAGGTTTAGAA 59 20 0 50.00 58.773 8.00 2.00 1.227 11 GCACACCCTGCAGGTTTAGA 58 20 0 55.00 61.236 8.00 4.00 1.236 12 CACTGAGGTCAGCACACCC 47 19 0 63.16 60.311 6.00 2.00 1.311 13 CCACTGAGGTCAGCACACC 46 19 0 63.16 60.311 6.00 4.00 1.311 14 CCCACTGAGGTCAGCACAC 45 19 0 63.16 60.311 6.00 2.00 1.311 15 CCTGCAGGTTTAGAAGGGG 64 19 0 57.89 59.673 8.00 0.00 1.327 16 CCCTGCAGGTTTAGAAGGG 63 19 0 57.89 59.673 8.00 2.00 1.327 17 GCAGGTTTAGAAGGGGTGTC 67 20 0 55.00 58.646 2.00 1.00 1.354 18 ACTGAGGTCAGCACACCCTG 48 20 0 60.00 61.355 6.00 2.00 1.355 19 CACTGAGGTCAGCACACCCT 47 20 0 60.00 61.355 6.00 2.00 1.355 20 GCAGGTTTAGAAGGGGTGTCT 67 21 0 52.38 59.630 3.00 0.00 1.370 21 CTGAGGTCAGCACACCCTG 49 19 0 63.16 60.461 6.00 2.00 1.461 22 ATCTGCACCCTGTAAACCCT 3 20 0 50.00 58.525 4.00 0.00 1.475 23 TTTAGAAGGGGTGTCTGGGT 72 20 0 50.00 58.498 3.00 0.00 1.502 24 GTTTAGAAGGGGTGTCTGGG 71 20 0 55.00 58.498 3.00 0.00 1.502 25 GGTTTAGAAGGGGTGTCTGG 70 20 0 55.00 58.498 3.00 0.00 1.502 26 AGGTTTAGAAGGGGTGTCTGG 69 21 0 52.38 59.488 3.00 0.00 1.512 27 CTGCAGGTTTAGAAGGGGTGT 65 21 0 52.38 60.534 6.00 0.00 1.534 28 ACACCCTGCAGGTTTAGAAGG 60 21 0 52.38 60.534 8.00 0.00 1.534 29 GTTTAGAAGGGGTGTCTGGGT 71 21 0 52.38 59.355 3.00 0.00 1.645 30 GCACACCCTGCAGGTTTAG 58 19 0 57.89 59.303 8.00 6.00 1.697 31 AGCACACCCTGCAGGTTTA 57 19 0 52.63 59.303 8.00 4.00 1.697 32 CACCCTGTAAACCCTGGCTC 8 20 0 60.00 61.826 3.00 1.00 1.826 33 CCTGTAAACCCTGGCTCCT 11 19 0 57.89 59.148 3.00 0.00 1.852 34 CTGCACCCTGTAAACCCTG 5 19 0 57.89 59.146 4.00 1.00 1.854 35 CCTGTAAACCCTGGCTCCTG 11 20 0 60.00 61.944 3.00 2.00 1.944 36 GACACCCCACTGAGGTCAG 40 19 0 63.16 59.044 7.00 6.00 1.956 37 ACCCTGTAAACCCTGGCTC 9 19 0 57.89 59.003 3.00 1.00 1.997 38 CTGTAAACCCTGGCTCCTGC 12 20 0 60.00 62.090 3.00 2.00 2.090 39 ACCCCACTGAGGTCAGCAC 43 19 0 63.16 61.171 7.00 1.00 2.171 40 ACACCCCACTGAGGTCAGC 41 19 0 63.16 61.171 7.00 4.00 2.171 41 TGTAAACCCTGGCTCCTGC 13 19 0 57.89 61.192 3.00 2.00 2.192 42 CAGCACACCCTGCAGGTTTA 56 20 0 55.00 62.212 8.00 4.00 2.212 43 CCCCACTGAGGTCAGCAC 44 18 0 66.67 60.252 6.00 1.00 2.252 44 ACCCCACTGAGGTCAGCA 43 18 0 61.11 60.252 7.00 2.00 2.252 45 CACCCCACTGAGGTCAGC 42 18 0 66.67 60.252 7.00 4.00 2.252 46 TTTAGAAGGGGTGTCTGGGTG 72 21 0 52.38 61.267 3.00 0.00 2.267 47 TGCAGGTTTAGAAGGGGTG 66 19 0 52.63 58.707 4.00 0.00 2.293 48 CAGGTTTAGAAGGGGTGTCTG 68 21 0 52.38 58.705 3.00 1.00 2.295 49 ATCTGCACCCTGTAAACCCTG 3 21 0 52.38 61.297 4.00 1.00 2.297 50 TAGAAGGGGTGTCTGGGTGC 74 20 0 60.00 62.397 3.00 2.00 2.397 51 TGCAGGTTTAGAAGGGGTGTC 66 21 0 52.38 61.411 4.00 1.00 2.411 52 GAGGTCAGCACACCCTGC 51 18 0 66.67 60.425 4.00 2.00 2.425 53 TAGAAGGGGTGTCTGGGTG 74 19 0 57.89 58.495 3.00 0.00 2.505 54 ACCCTGTAAACCCTGGCTCC 9 20 0 60.00 62.523 3.00 2.00 2.523 55 CCCTGTAAACCCTGGCTCCT 10 20 0 60.00 62.632 3.00 0.00 2.632 56 TGAGGTCAGCACACCCTG 50 18 0 61.11 59.315 4.00 1.00 2.685 57 CCCACTGAGGTCAGCACA 45 18 0 61.11 59.315 6.00 3.00 2.685 58 CTGTAAACCCTGGCTCCTG 12 19 0 57.89 58.300 3.00 2.00 2.700 59 AGCACACCCTGCAGGTTT 57 18 0 55.56 59.261 8.00 6.00 2.739 60 CTGCACCCTGTAAACCCTGG 5 20 0 60.00 62.773 4.00 3.00 2.773 61 GTAAACCCTGGCTCCTGCCT 14 20 0 60.00 62.773 3.00 0.00 2.773 62 ACTGAGGTCAGCACACCCT 48 19 0 57.89 58.217 6.00 2.00 2.783 63 CCCTGTAAACCCTGGCTCC 10 19 0 63.16 61.789 3.00 2.00 2.789 64 GGGACACCCCACTGAGGT 38 18 0 66.67 60.812 7.00 7.00 2.812 65 TCTGCACCCTGTAAACCCT 4 19 0 52.63 58.144 4.00 0.00 2.856 66 TGCACCCTGTAAACCCTGG 6 19 0 57.89 61.900 4.00 3.00 2.900 67 CAGCACACCCTGCAGGTT 56 18 0 61.11 60.905 8.00 8.00 2.905 68 GTAAACCCTGGCTCCTGCC 14 19 0 63.16 61.937 3.00 2.00 2.937 69 GGTTTAGAAGGGGTGTCTGGG 70 21 0 57.14 61.965 3.00 0.00 2.965 70 GGACACCCCACTGAGGTCAG 39 20 0 65.00 62.981 7.00 6.00 2.981 71 GGACACCCCACTGAGGTCA 39 19 0 63.16 62.043 7.00 4.00 3.043 72 TAAACCCTGGCTCCTGCCT 15 19 0 57.89 62.055 3.00 0.00 3.055 73 CACCCTGTAAACCCTGGC 8 18 0 61.11 58.930 3.00 2.00 3.070 74 GCACCCTGTAAACCCTGG 7 18 0 61.11 58.930 3.00 3.00 3.070 75 AGCACACCCTGCAGGTTTAGA 57 21 0 52.38 62.124 8.00 4.00 3.124 76 TAAACCCTGGCTCCTGCC 15 18 0 61.11 61.147 3.00 2.00 3.147 77 GGACACCCCACTGAGGTC 39 18 0 66.67 58.806 7.00 5.00 3.194 78 CAGCACACCCTGCAGGTTT 56 19 0 57.89 62.292 8.00 6.00 3.292 79 GTCAGCACACCCTGCAGGT 54 19 0 63.16 62.397 8.00 6.00 3.397 80 TTTAGAAGGGGTGTCTGGG 72 19 0 52.63 57.569 3.00 0.00 3.431 81 GCACACCCTGCAGGTTTAGAA 58 21 0 52.38 62.477 8.00 2.00 3.477 82 AGAAGGGGTGTCTGGGTGC 75 19 0 63.16 62.484 3.00 2.00 3.484 83 AGGTCAGCACACCCTGCAG 52 19 0 63.16 62.524 6.00 6.00 3.524 84 TCAGCACACCCTGCAGGT 55 18 0 61.11 61.534 8.00 6.00 3.534 85 GTCAGCACACCCTGCAGG 54 18 0 66.67 61.534 8.00 8.00 3.534 86 GGTCAGCACACCCTGCAG 53 18 0 66.67 61.534 6.00 6.00 3.534 87 AGGTCAGCACACCCTGCA 52 18 0 61.11 61.534 4.00 4.00 3.534 88 GAAGGGGTGTCTGGGTGC 76 18 0 66.67 61.536 2.00 2.00 3.536 89 ATCTGCACCCTGTAAACCC 3 19 0 52.63 57.435 4.00 0.00 3.565 90 AGAAGGGGTGTCTGGGTG 75 18 0 61.11 58.416 3.00 0.00 3.584 91 GTAAACCCTGGCTCCTGC 14 18 0 61.11 58.230 3.00 2.00 3.770 92 CACACCCTGCAGGTTTAGA 59 19 0 52.63 57.225 8.00 3.00 3.775 93 GCACCCTGTAAACCCTGGC 7 19 0 63.16 62.779 3.00 2.00 3.779 94 GCACACCCTGCAGGTTTA 58 18 0 55.56 58.186 8.00 4.00 3.814 95 GGGACACCCCACTGAGGTC 38 19 0 68.42 62.829 7.00 5.00 3.829 96 TCAGCACACCCTGCAGGTT 55 19 0 57.89 62.920 8.00 8.00 3.920 97 CCTGTAAACCCTGGCTCC 11 18 0 61.11 58.071 3.00 2.00 3.929 98 CCCTGTAAACCCTGGCTC 10 18 0 61.11 58.071 3.00 1.00 3.929 99 TGCACCCTGTAAACCCTG 6 18 0 55.56 58.020 4.00 1.00 3.980 100 GTTTAGAAGGGGTGTCTGGGTG 71 22 0 54.55 61.999 3.00 0.00 3.999 101 CAGGTTTAGAAGGGGTGTCTGG 68 22 0 54.55 62.108 3.00 0.00 4.108 102 AAACCCTGGCTCCTGCCT 16 18 0 61.11 62.126 3.00 0.00 4.126 103 GACACCCCACTGAGGTCA 40 18 0 61.11 57.806 7.00 4.00 4.194 104 GCAGGTTTAGAAGGGGTGTCTG 67 22 0 54.55 62.245 3.00 1.00 4.245 105 TGCAGGTTTAGAAGGGGTGTCT 66 22 0 50.00 62.245 4.00 0.00 4.245 106 CTGCAGGTTTAGAAGGGGTGTC 65 22 0 54.55 62.245 6.00 1.00 4.245 107 ACCCTGGCTCCTGCCTCT 18 18 0 66.67 62.344 3.00 0.00 4.344 108 GGTTTAGAAGGGGTGTCTGGGT 70 22 0 54.55 62.650 3.00 0.00 4.650 109 AACCCTGGCTCCTGCCTC 17 18 0 66.67 62.728 3.00 0.00 4.728 110 AGGTTTAGAAGGGGTGTCTGGG 69 22 0 54.55 62.752 3.00 0.00 4.752 111 CTGAGGTCAGCACACCCT 49 18 0 61.11 57.145 6.00 2.00 4.855 112 TGTAAACCCTGGCTCCTG 13 18 0 55.56 57.142 3.00 2.00 4.858 113 ACCCTGTAAACCCTGGCT 9 18 0 55.56 57.001 3.00 3.00 4.999