ACCEPTABLE RIGHT PRIMERS 1-based # self self qual- # sequence start ln N GC% Tm any end lity 0 GATCCTGCTGAAGCAAATGG 175 20 0 50.00 60.744 6.00 1.00 0.744 1 ATCCTGCTGAAGCAAATGGA 174 20 0 45.00 60.744 6.00 1.00 0.744 2 CTGAAGCAAATGGACGTGG 168 19 0 52.63 60.247 4.00 0.00 1.247 3 TCCTGCTGAAGCAAATGGAC 173 20 0 50.00 61.329 6.00 1.00 1.329 4 CTGCTGAAGCAAATGGACGT 171 20 0 50.00 61.375 6.00 4.00 1.375 5 GTCACCAAATGCACCCAGAC 103 20 0 55.00 61.396 4.00 1.00 1.396 6 TGCTGAAGCAAATGGACGT 170 19 0 47.37 60.406 6.00 4.00 1.406 7 GCTGAAGCAAATGGACGTG 169 19 0 52.63 60.406 4.00 2.00 1.406 8 TCCTGCTGAAGCAAATGGA 173 19 0 47.37 60.487 6.00 1.00 1.487 9 GTCACCAAATGCACCCAGA 103 19 0 52.63 60.527 4.00 0.00 1.527 10 TCACCAAATGCACCCAGAC 102 19 0 52.63 60.527 4.00 1.00 1.527 11 CTGCTGAAGCAAATGGACG 171 19 0 52.63 60.546 6.00 2.00 1.546 12 CCTGCTGAAGCAAATGGAC 172 19 0 52.63 59.384 6.00 1.00 1.616 13 ACCAAATGCACCCAGACAC 100 19 0 52.63 59.383 4.00 0.00 1.617 14 AGCAAATGGACGTGGCAT 164 18 0 50.00 60.080 4.00 4.00 2.080 15 ATCCTGCTGAAGCAAATGG 174 19 0 47.37 58.811 6.00 1.00 2.189 16 GAAGCAAATGGACGTGGC 166 18 0 55.56 60.210 4.00 2.00 2.210 17 CTGCGGTGTCACCAAATG 110 18 0 55.56 59.652 7.00 3.00 2.348 18 GTGTCACCAAATGCACCCAG 105 20 0 55.00 62.415 5.00 1.00 2.415 19 CACCAAATGCACCCAGACAC 101 20 0 55.00 62.415 4.00 0.00 2.415 20 TCACCAAATGCACCCAGA 102 18 0 50.00 59.577 4.00 0.00 2.423 21 GTGTCACCAAATGCACCCA 105 19 0 52.63 61.452 5.00 0.00 2.452 22 AAGCAAATGGACGTGGCAT 165 19 0 47.37 61.465 4.00 4.00 2.465 23 CGGTGTCACCAAATGCAC 107 18 0 55.56 59.492 7.00 2.00 2.508 24 TGCTGAAGCAAATGGACG 170 18 0 50.00 59.490 6.00 2.00 2.510 25 ATCCTGCTGAAGCAAATGGAC 174 21 0 47.62 61.530 6.00 1.00 2.530 26 CCTGCGGTGTCACCAAAT 111 18 0 55.56 60.534 7.00 3.00 2.534 27 TGTCACCAAATGCACCCA 104 18 0 50.00 60.535 4.00 0.00 2.535 28 TGTCACCAAATGCACCCAG 104 19 0 52.63 61.589 4.00 1.00 2.589 29 CACCAAATGCACCCAGACA 101 19 0 52.63 61.589 4.00 0.00 2.589 30 TGAAGCAAATGGACGTGG 167 18 0 50.00 59.165 4.00 0.00 2.835 31 CAAATGGACGTGGCATCC 162 18 0 55.56 60.915 4.00 3.00 2.915 32 AGCAAATGGACGTGGCATC 164 19 0 52.63 62.050 4.00 2.00 3.050 33 GCAAATGGACGTGGCATC 163 18 0 55.56 61.080 4.00 2.00 3.080 34 AAGCAAATGGACGTGGCA 165 18 0 50.00 61.248 4.00 3.00 3.248 35 GGTGTCACCAAATGCACCC 106 19 0 57.89 62.254 7.00 1.00 3.254 36 GATCCTGCTGAAGCAAATGGA 175 21 0 47.62 62.559 6.00 1.00 3.559 37 TCCTGCTGAAGCAAATGG 173 18 0 50.00 58.427 6.00 1.00 3.573 38 CCTGCTGAAGCAAATGGA 172 18 0 50.00 58.427 6.00 0.00 3.573 39 GTCACCAAATGCACCCAG 103 18 0 55.56 58.389 4.00 1.00 3.611 40 CACCAAATGCACCCAGAC 101 18 0 55.56 58.389 4.00 1.00 3.611 41 ACCAAATGCACCCAGACA 100 18 0 50.00 58.389 4.00 0.00 3.611 42 CTCTGCCTGCGGTGTCAC 116 18 0 66.67 61.691 5.00 5.00 3.691 43 GGTGTCACCAAATGCACC 106 18 0 55.56 58.218 7.00 3.00 3.782 44 GTGTCACCAAATGCACCC 105 18 0 55.56 58.218 5.00 0.00 3.782 45 GCTGAAGCAAATGGACGT 169 18 0 50.00 57.302 4.00 4.00 4.698 46 TGCGGTGTCACCAAATGC 109 18 0 55.56 62.807 7.00 2.00 4.807 47 GCGGTGTCACCAAATGCA 108 18 0 55.56 62.807 7.00 4.00 4.807 48 AAATGGACGTGGCATCCG 161 18 0 55.56 62.891 4.00 2.00 4.891