ACCEPTABLE LEFT PRIMERS 1-based # self self hair- qual- # sequence start ln N GC% Tm any_th end_th pin lity 0 GCTGTTTCACACTTACGGAA 8 20 0 45.00 57.204 0.00 0.00 0.00 2.796 1 GCTGTTTCACACTTACGGAAA 8 21 0 42.86 57.949 0.00 0.00 0.00 3.051 2 GCTGTTTCACACTTACGGAAATG 8 23 0 43.48 59.687 0.00 0.00 34.24 3.313 3 TGTTTCACACTTACGGAAATGGA 10 23 0 39.13 59.482 0.00 0.00 34.24 3.518 4 GCTGTTTCACACTTACGGAAAT 8 22 0 40.91 58.429 0.00 0.00 34.24 3.571 5 CTTAGCGTGCGTGGGGTC 142 18 0 66.67 61.698 0.00 0.00 0.00 3.698 6 CTGTTTCACACTTACGGAAATGG 9 23 0 43.48 59.129 0.00 0.00 34.24 3.871 7 TGTTTCACACTTACGGAAATGG 10 22 0 40.91 58.094 0.00 0.00 34.24 3.906 8 GTTTCACACTTACGGAAATGGA 11 22 0 40.91 57.832 0.00 0.00 34.24 4.168 9 CTGTTTCACACTTACGGAAATGGA 9 24 0 41.67 60.417 0.00 0.00 34.24 4.417 10 GCTGTTTCACACTTACGGAAATGG 8 24 0 45.83 61.766 0.00 0.00 34.24 5.766 11 GCTGTTTCACACTTACGGAAATGGA 8 25 0 44.00 62.926 0.00 0.00 34.24 7.926