ACCEPTABLE LEFT PRIMERS 1-based # self self hair- qual- # sequence start ln N GC% Tm any_th end_th pin lity 0 TGCAGGTTTAGAAGGGGTGT 66 20 0 50.00 59.801 0.00 0.00 0.00 0.199 1 CCTGCAGGTTTAGAAGGGGT 64 20 0 55.00 60.243 26.16 0.00 0.00 0.243 2 TTAGAAGGGGTGTCTGGGTG 73 20 0 55.00 59.583 0.00 0.00 0.00 0.417 3 ATCTGCACCCTGTAAACCCT 3 20 0 50.00 59.577 0.00 0.00 0.00 0.423 4 TCTGCACCCTGTAAACCCTG 4 20 0 55.00 60.533 0.00 0.00 0.00 0.533 5 GCAGGTTTAGAAGGGGTGTC 67 20 0 55.00 59.095 0.00 0.00 0.00 0.905 6 CTGCAGGTTTAGAAGGGGTG 65 20 0 55.00 59.093 6.10 0.00 0.00 0.907 7 CCTGTAAACCCTGGCTCCTG 11 20 0 60.00 60.964 0.00 0.00 0.00 0.964 8 CCCTGCAGGTTTAGAAGGGG 63 20 0 60.00 60.969 31.21 9.70 44.08 0.969 9 AGGTTTAGAAGGGGTGTCTGG 69 21 0 52.38 59.961 0.00 0.00 0.00 1.039 10 ACCCTGTAAACCCTGGCTC 9 19 0 57.89 59.860 0.00 0.00 0.00 1.140 11 CACCCTGTAAACCCTGGCT 8 19 0 57.89 60.168 0.00 0.00 0.00 1.168 12 TTTAGAAGGGGTGTCTGGGT 72 20 0 50.00 58.826 0.00 0.00 0.00 1.174 13 TTTAGAAGGGGTGTCTGGGTG 72 21 0 52.38 60.242 0.00 0.00 0.00 1.242 14 GTTTAGAAGGGGTGTCTGGGT 71 21 0 52.38 60.242 0.00 0.00 0.00 1.242 15 CACCCTGTAAACCCTGGCTC 8 20 0 60.00 61.249 0.00 0.00 0.00 1.249 16 CCTGTAAACCCTGGCTCCT 11 19 0 57.89 59.550 0.00 0.00 0.00 1.450 17 TGCACCCTGTAAACCCTGG 6 19 0 57.89 60.474 0.00 0.00 0.00 1.474 18 CTGCACCCTGTAAACCCTGG 5 20 0 60.00 61.536 0.00 0.00 0.00 1.536 19 TGTAAACCCTGGCTCCTGC 13 19 0 57.89 60.548 0.00 0.00 38.76 1.548 20 GTTTAGAAGGGGTGTCTGGG 71 20 0 55.00 58.433 0.00 0.00 0.00 1.567 21 GGTTTAGAAGGGGTGTCTGG 70 20 0 55.00 58.433 0.00 0.00 0.00 1.567 22 GCAGGTTTAGAAGGGGTGTCT 67 21 0 52.38 60.589 0.00 0.00 0.00 1.589 23 CTGTAAACCCTGGCTCCTGC 12 20 0 60.00 61.600 0.00 0.00 38.76 1.600 24 CCCTGTAAACCCTGGCTCC 10 19 0 63.16 60.625 0.00 0.00 0.00 1.625 25 TGCAGGTTTAGAAGGGGTGTC 66 21 0 52.38 60.864 0.00 0.00 0.00 1.864 26 CTGCAGGTTTAGAAGGGGTGT 65 21 0 52.38 60.866 6.10 0.00 0.00 1.866 27 CTGAGGTCAGCACACCCTG 49 19 0 63.16 60.916 1.06 0.00 37.75 1.916 28 TCTGCACCCTGTAAACCCT 4 19 0 52.63 59.079 0.00 0.00 0.00 1.921 29 GGTTTAGAAGGGGTGTCTGGG 70 21 0 57.14 60.934 0.00 0.00 0.00 1.934 30 ATCTGCACCCTGTAAACCCTG 3 21 0 52.38 60.936 0.00 0.00 0.00 1.936 31 CTGCACCCTGTAAACCCTG 5 19 0 57.89 58.965 0.00 0.00 0.00 2.035 32 CAGGTTTAGAAGGGGTGTCTG 68 21 0 52.38 58.866 0.00 0.00 0.00 2.134 33 TAGAAGGGGTGTCTGGGTGC 74 20 0 60.00 62.142 0.00 0.00 0.00 2.142 34 TAGAAGGGGTGTCTGGGTG 74 19 0 57.89 58.854 0.00 0.00 0.00 2.146 35 GGACACCCCACTGAGGTC 39 18 0 66.67 59.846 0.00 0.00 35.35 2.154 36 CCCTGTAAACCCTGGCTCCT 10 20 0 60.00 62.160 0.00 0.00 0.00 2.160 37 TGAGGTCAGCACACCCTG 50 18 0 61.11 59.766 1.06 0.00 37.75 2.234 38 ACCCTGTAAACCCTGGCTCC 9 20 0 60.00 62.448 0.00 0.00 0.00 2.448 39 CCTGCAGGTTTAGAAGGGGTG 64 21 0 57.14 61.548 26.16 0.00 0.00 2.548 40 CTGAGGTCAGCACACCCT 49 18 0 61.11 59.443 1.06 0.00 37.75 2.557 41 AGGTTTAGAAGGGGTGTCTG 69 20 0 50.00 57.384 0.00 0.00 0.00 2.616 42 CAGGTTTAGAAGGGGTGTCT 68 20 0 50.00 57.384 0.00 0.00 0.00 2.616 43 CTGTAAACCCTGGCTCCTG 12 19 0 57.89 58.355 0.00 0.00 0.00 2.645 44 CCTGCAGGTTTAGAAGGGG 64 19 0 57.89 58.343 26.16 0.00 0.00 2.657 45 CCCTGCAGGTTTAGAAGGG 63 19 0 57.89 58.343 31.21 10.50 42.19 2.657 46 GGACACCCCACTGAGGTCA 39 19 0 63.16 61.780 0.00 0.00 35.35 2.780 47 GAAGGGGTGTCTGGGTGC 76 18 0 66.67 60.880 0.00 0.00 0.00 2.880 48 GCACCCTGTAAACCCTGGC 7 19 0 63.16 61.895 0.00 0.00 0.00 2.895 49 TTAGAAGGGGTGTCTGGGT 73 19 0 52.63 58.044 0.00 0.00 0.00 2.956 50 GACACCCCACTGAGGTCA 40 18 0 61.11 59.037 0.00 0.00 35.35 2.963 51 AGAAGGGGTGTCTGGGTG 75 18 0 61.11 59.025 0.00 0.00 0.00 2.975 52 ATCTGCACCCTGTAAACCC 3 19 0 52.63 57.946 0.00 0.00 0.00 3.054 53 TGCAGGTTTAGAAGGGGTG 66 19 0 52.63 57.872 0.00 0.00 0.00 3.128 54 GCAGGTTTAGAAGGGGTGT 67 19 0 52.63 57.870 0.00 0.00 0.00 3.130 55 CAGGTTTAGAAGGGGTGTCTGG 68 22 0 54.55 61.229 0.00 0.00 0.00 3.229 56 ACCCTGTAAACCCTGGCT 9 18 0 55.56 58.594 0.00 0.00 0.00 3.406 57 GTAAACCCTGGCTCCTGC 14 18 0 61.11 58.583 0.00 0.00 38.76 3.417 58 CTGCAGGTTTAGAAGGGGT 65 19 0 52.63 57.556 6.10 0.00 0.00 3.444 59 AGAAGGGGTGTCTGGGTGC 75 19 0 63.16 62.469 0.00 0.00 0.00 3.469 60 GTTTAGAAGGGGTGTCTGGGTG 71 22 0 54.55 61.489 0.00 0.00 0.00 3.489 61 CACCCTGTAAACCCTGGC 8 18 0 61.11 58.494 0.00 0.00 0.00 3.506 62 GCACCCTGTAAACCCTGG 7 18 0 61.11 58.494 0.00 0.00 0.00 3.506 63 TTAGAAGGGGTGTCTGGGTGC 73 21 0 57.14 62.677 0.00 0.00 0.00 3.677 64 CCCTGCAGGTTTAGAAGGGGT 63 21 0 57.14 62.699 31.21 0.00 44.08 3.699 65 GAAGGGGTGTCTGGGTGCA 76 19 0 63.16 62.766 0.00 0.00 0.00 3.766 66 GCAGGTTTAGAAGGGGTGTCTG 67 22 0 54.55 61.808 0.00 0.00 0.00 3.808 67 CTGCAGGTTTAGAAGGGGTGTC 65 22 0 54.55 61.808 6.10 0.00 0.00 3.808 68 TCTGCACCCTGTAAACCCTGG 4 21 0 57.14 62.952 0.00 0.00 0.00 3.952 69 CTCCTGCCTCTCCGGGAC 25 18 0 72.22 62.066 0.00 0.00 36.14 4.066 70 CCTGTAAACCCTGGCTCC 11 18 0 61.11 57.847 0.00 0.00 0.00 4.153 71 CCCTGTAAACCCTGGCTC 10 18 0 61.11 57.847 0.00 0.00 0.00 4.153 72 TGCAGGTTTAGAAGGGGTGTCT 66 22 0 50.00 62.257 0.00 0.00 0.00 4.257 73 AGGTTTAGAAGGGGTGTCTGGG 69 22 0 54.55 62.326 0.00 0.00 0.00 4.326 74 TGCACCCTGTAAACCCTG 6 18 0 55.56 57.673 0.00 0.00 0.00 4.327 75 GGTTTAGAAGGGGTGTCTGGGT 70 22 0 54.55 62.588 0.00 0.00 0.00 4.588 76 TCTGCACCCTGTAAACCC 4 18 0 55.56 57.349 0.00 0.00 0.00 4.651 77 CTGCACCCTGTAAACCCT 5 18 0 55.56 57.340 0.00 0.00 0.00 4.660 78 CTGCCTCTCCGGGACACC 28 18 0 72.22 62.688 0.00 0.00 0.00 4.688 79 CCTGCCTCTCCGGGACAC 27 18 0 72.22 62.688 0.00 0.00 33.37 4.688 80 TAGAAGGGGTGTCTGGGT 74 18 0 55.56 57.173 0.00 0.00 0.00 4.827 81 TCCTGCCTCTCCGGGACA 26 18 0 66.67 62.937 0.00 0.00 36.14 4.937 82 TGTAAACCCTGGCTCCTG 13 18 0 55.56 57.016 0.00 0.00 0.00 4.984