ACCEPTABLE RIGHT PRIMERS 1-based # self self hair- qual- # sequence start ln N GC% Tm any_th end_th pin lity 0 GTCACCAAATGCACCCAGAC 103 20 0 55.00 60.312 0.00 0.00 0.00 0.312 1 GAAGCAAATGGACGTGGCAT 166 20 0 50.00 60.383 0.00 0.00 0.00 0.383 2 AAGCAAATGGACGTGGCATC 165 20 0 50.00 60.383 0.00 0.00 0.00 0.383 3 GTGTCACCAAATGCACCCAG 105 20 0 55.00 60.595 0.00 0.00 33.06 0.595 4 CACCAAATGCACCCAGACAC 101 20 0 55.00 60.595 0.00 0.00 0.00 0.595 5 TGTCACCAAATGCACCCAGA 104 20 0 50.00 60.753 0.00 0.00 0.00 0.753 6 TCACCAAATGCACCCAGACA 102 20 0 50.00 60.753 0.00 0.00 0.00 0.753 7 GCTGAAGCAAATGGACGTGG 169 20 0 55.00 61.007 0.00 0.00 41.90 1.007 8 CTGAAGCAAATGGACGTGGC 168 20 0 55.00 61.007 0.00 0.00 0.00 1.007 9 TGAAGCAAATGGACGTGGC 167 19 0 52.63 59.946 0.00 0.00 0.00 1.054 10 GAAGCAAATGGACGTGGCA 166 19 0 52.63 59.946 0.00 0.00 0.00 1.054 11 GGTGTCACCAAATGCACCC 106 19 0 57.89 60.243 18.20 0.00 44.57 1.243 12 AGCAAATGGACGTGGCATC 164 19 0 52.63 59.721 0.00 0.00 0.00 1.279 13 CCTGCGGTGTCACCAAATG 111 19 0 57.89 60.316 22.47 7.16 39.26 1.316 14 CAAATGGACGTGGCATCCG 162 19 0 57.89 60.461 0.00 0.00 39.91 1.461 15 GTGTCACCAAATGCACCCA 105 19 0 52.63 59.490 0.00 0.00 33.06 1.510 16 CGGTGTCACCAAATGCACC 107 19 0 57.89 60.609 22.47 0.00 45.61 1.609 17 AAGCAAATGGACGTGGCAT 165 19 0 47.37 59.260 0.00 0.00 0.00 1.740 18 TGAAGCAAATGGACGTGGCA 167 20 0 50.00 61.744 0.00 0.00 0.00 1.744 19 GCAAATGGACGTGGCATCC 163 19 0 57.89 60.756 0.00 0.69 36.99 1.756 20 ACCAAATGCACCCAGACACC 100 20 0 55.00 61.760 0.00 0.00 0.00 1.760 21 TGTCACCAAATGCACCCAG 104 19 0 52.63 59.185 0.00 0.00 0.00 1.815 22 CACCAAATGCACCCAGACA 101 19 0 52.63 59.185 0.00 0.00 0.00 1.815 23 ACCAAATGCACCCAGACAC 100 19 0 52.63 59.185 0.00 0.00 0.00 1.815 24 CTGCGGTGTCACCAAATGC 110 19 0 57.89 60.971 22.47 16.94 39.26 1.971 25 GGTGTCACCAAATGCACCCA 106 20 0 55.00 62.042 18.20 0.00 44.57 2.042 26 GTCACCAAATGCACCCAGA 103 19 0 52.63 58.882 0.00 0.00 0.00 2.118 27 TCACCAAATGCACCCAGAC 102 19 0 52.63 58.882 0.00 0.00 0.00 2.118 28 TGCGGTGTCACCAAATGC 109 18 0 55.56 59.855 22.47 16.94 39.26 2.145 29 GCGGTGTCACCAAATGCA 108 18 0 55.56 59.855 22.47 0.00 39.26 2.145 30 AGCAAATGGACGTGGCATCC 164 20 0 55.00 62.235 0.00 0.69 36.99 2.235 31 GCGGTGTCACCAAATGCAC 108 19 0 57.89 61.259 22.47 0.00 39.26 2.259 32 GAAGCAAATGGACGTGGCATC 166 21 0 52.38 61.372 0.00 0.00 0.00 2.372 33 GCTGAAGCAAATGGACGTG 169 19 0 52.63 58.492 0.00 0.00 41.90 2.508 34 CTGCGGTGTCACCAAATGCA 110 20 0 55.00 62.705 22.47 20.34 39.26 2.705 35 TGCGGTGTCACCAAATGCA 109 19 0 52.63 61.741 22.47 19.30 39.26 2.741 36 TGCGGTGTCACCAAATGCAC 109 20 0 55.00 62.978 22.47 3.23 39.26 2.978 37 AAATGGACGTGGCATCCG 161 18 0 55.56 58.992 0.00 0.00 39.91 3.008 38 GTGTCACCAAATGCACCCAGA 105 21 0 52.38 62.021 0.00 0.00 33.06 3.021 39 TGTCACCAAATGCACCCAGAC 104 21 0 52.38 62.021 0.00 0.00 0.00 3.021 40 GTCACCAAATGCACCCAGACA 103 21 0 52.38 62.021 0.00 0.00 0.00 3.021 41 TCACCAAATGCACCCAGACAC 102 21 0 52.38 62.021 0.00 0.00 0.00 3.021 42 TGAAGCAAATGGACGTGGCAT 167 21 0 47.62 62.089 0.00 0.00 0.00 3.089 43 CCTGCGGTGTCACCAAAT 111 18 0 55.56 58.824 22.47 0.00 39.26 3.176 44 GCCTGCGGTGTCACCAAAT 112 19 0 57.89 62.180 22.47 0.00 39.26 3.180 45 CTGAAGCAAATGGACGTGG 168 19 0 52.63 57.813 0.00 0.00 0.00 3.187 46 AAGCAAATGGACGTGGCA 165 18 0 50.00 58.736 0.00 0.00 0.00 3.264 47 AAATGGACGTGGCATCCGC 161 19 0 57.89 62.311 0.00 0.00 39.91 3.311 48 AGCAAATGGACGTGGCAT 164 18 0 50.00 58.490 0.00 0.00 0.00 3.510 49 CTCTGCCTGCGGTGTCAC 116 18 0 66.67 61.628 0.00 0.00 0.00 3.628 50 CTGAAGCAAATGGACGTGGCA 168 21 0 52.38 62.667 0.00 0.00 0.00 3.667 51 AATGGACGTGGCATCCGC 160 18 0 61.11 61.723 0.00 0.00 39.91 3.723 52 AAGCAAATGGACGTGGCATCC 165 21 0 52.38 62.752 0.00 0.69 36.99 3.752 53 GCCTGCGGTGTCACCAAA 112 18 0 61.11 61.808 22.47 8.65 39.26 3.808 54 GCAAATGGACGTGGCATC 163 18 0 55.56 58.087 0.00 0.00 0.00 3.913 55 GGTGTCACCAAATGCACCCAG 106 21 0 57.14 62.953 18.20 0.00 44.57 3.953 56 CACCAAATGCACCCAGACACC 101 21 0 57.14 62.953 0.00 0.00 0.00 3.953 57 GAAGCAAATGGACGTGGC 166 18 0 55.56 58.010 0.00 0.00 0.00 3.990 58 CGGTGTCACCAAATGCAC 107 18 0 55.56 57.936 22.47 0.00 39.26 4.064 59 TGTCACCAAATGCACCCA 104 18 0 50.00 57.903 0.00 0.00 0.00 4.097 60 CTGCGGTGTCACCAAATG 110 18 0 55.56 57.620 22.47 7.16 39.26 4.380 61 ACCAAATGCACCCAGACA 100 18 0 50.00 57.569 0.00 0.00 0.00 4.431 62 GGTGTCACCAAATGCACC 106 18 0 55.56 57.506 18.20 0.00 44.15 4.494 63 GTGTCACCAAATGCACCC 105 18 0 55.56 57.506 0.00 0.00 33.06 4.494 64 CAAATGGACGTGGCATCC 162 18 0 55.56 57.365 0.00 0.69 36.99 4.635 65 TCACCAAATGCACCCAGA 102 18 0 50.00 57.243 0.00 0.00 0.00 4.757 66 GTCACCAAATGCACCCAG 103 18 0 55.56 57.183 0.00 0.00 0.00 4.817 67 CACCAAATGCACCCAGAC 101 18 0 55.56 57.183 0.00 0.00 0.00 4.817 68 CCTCTGCCTGCGGTGTCA 117 18 0 66.67 62.919 0.00 0.00 0.00 4.919 69 TGAAGCAAATGGACGTGGCATC 167 22 0 50.00 62.949 0.00 0.00 0.00 4.949 70 CTGAAGCAAATGGACGTGGCAT 168 22 0 50.00 62.956 0.00 0.00 0.00 4.956