ACCEPTABLE LEFT PRIMERS 1-based # self self hair- qual- # sequence start ln N GC% Tm any_th end_th pin lity 0 TCGCAATCAAATTTTTTCTATGACCT 37 26 0 30.77 59.341 0.00 0.00 0.00 6.659 1 TCGCAATCAAATTTTTTCTATGACC 37 25 0 32.00 58.114 0.00 0.00 0.00 6.886 2 ATCGCAATCAAATTTTTTCTATGACCT 36 27 0 29.63 59.695 0.00 0.00 0.00 7.305 3 TCGCAATCAAATTTTTTCTATGACCTG 37 27 0 33.33 60.394 0.00 0.00 0.00 7.394 4 ATCGCAATCAAATTTTTTCTATGACC 36 26 0 30.77 58.519 0.00 0.00 0.00 7.481 5 AATCGCAATCAAATTTTTTCTATGACC 35 27 0 29.63 59.061 0.00 0.00 0.00 7.939 6 TGAATCGCAATCAAATTTTTTCTATGA 33 27 0 25.93 58.301 0.00 0.00 0.00 8.699 7 TGAATCGCAATCAAATTTTTTCTATG 33 26 0 26.92 57.093 0.00 0.00 0.00 8.907 8 AATCGCAATCAAATTTTTTCTATGAC 35 26 0 26.92 57.092 0.00 0.00 0.00 8.908 9 GAATCGCAATCAAATTTTTTCTATGAC 34 27 0 29.63 58.026 0.00 0.00 0.00 8.974 10 ATGAATCGCAATCAAATTTTTTCTATG 32 27 0 25.93 57.518 0.00 0.00 0.00 9.482