ACCEPTABLE RIGHT PRIMERS 1-based # self self hair- qual- # sequence start ln N GC% Tm any_th end_th pin lity 0 CGTGCTTTGCGTTTTGGAAG 127 20 0 50.00 60.034 0.00 0.00 0.00 0.034 1 GTGCTTTGCGTTTTGGAAGT 126 20 0 45.00 58.977 0.00 0.00 0.00 1.023 2 GAGCGTGCTTTGCGTTTTG 130 19 0 52.63 60.026 0.00 0.00 37.77 1.026 3 GTGCTTTGCGTTTTGGAAGTT 126 21 0 42.86 59.636 0.00 0.00 0.00 1.364 4 TGCTTTGCGTTTTGGAAGTT 125 20 0 40.00 58.246 0.00 0.00 0.00 1.754 5 GCGTGCTTTGCGTTTTGGAA 128 20 0 50.00 62.034 0.00 0.00 0.00 2.034 6 GCGTGCTTTGCGTTTTGG 128 18 0 55.56 59.941 0.00 0.00 0.00 2.059 7 CGTGCTTTGCGTTTTGGAA 127 19 0 47.37 58.931 0.00 0.00 0.00 2.069 8 GGAGCGTGCTTTGCGTTTT 131 19 0 52.63 61.186 0.00 0.00 37.77 2.186 9 GATCGGGAGCGTGCTTTG 136 18 0 61.11 59.784 0.00 0.00 0.00 2.216 10 GGAGCGTGCTTTGCGTTTTG 131 20 0 55.00 62.422 0.00 0.00 37.77 2.422 11 GAGCGTGCTTTGCGTTTTGG 130 20 0 55.00 62.422 0.00 0.00 37.77 2.422 12 GCGTGCTTTGCGTTTTGGA 128 19 0 52.63 61.472 0.00 0.00 0.00 2.472 13 AGCGTGCTTTGCGTTTTGG 129 19 0 52.63 61.476 0.00 0.00 37.77 2.476 14 GTGCTTTGCGTTTTGGAAGTTA 126 22 0 40.91 59.468 0.00 0.00 0.00 2.532 15 GGAGCGTGCTTTGCGTTT 131 18 0 55.56 60.548 0.00 0.00 37.77 2.548 16 CGTGCTTTGCGTTTTGGAAGT 127 21 0 47.62 61.698 0.00 0.00 0.00 2.698 17 TGCTTTGCGTTTTGGAAGTTAG 125 22 0 40.91 59.209 0.00 0.00 0.00 2.791 18 TGCTTTGCGTTTTGGAAGTTA 125 21 0 38.10 58.130 0.00 0.00 0.00 2.870 19 AGCGTGCTTTGCGTTTTGGA 129 20 0 50.00 62.908 0.00 0.00 37.77 2.908 20 GCTTTGCGTTTTGGAAGTTAGA 124 22 0 40.91 58.951 0.00 0.00 0.00 3.049 21 AGCGTGCTTTGCGTTTTG 129 18 0 50.00 58.874 0.00 0.00 37.77 3.126 22 GCTTTGCGTTTTGGAAGTTAGAC 124 23 0 43.48 60.176 0.00 0.00 0.00 3.176 23 GTGCTTTGCGTTTTGGAAGTTAG 126 23 0 43.48 60.419 0.00 0.00 0.00 3.419 24 GAGCGTGCTTTGCGTTTT 130 18 0 50.00 58.568 0.00 0.00 37.77 3.432 25 GCTTTGCGTTTTGGAAGTTAG 124 21 0 42.86 57.515 0.00 0.00 0.00 3.485 26 TGCTTTGCGTTTTGGAAGT 125 19 0 42.11 57.471 0.00 0.00 0.00 3.529 27 TGCTTTGCGTTTTGGAAGTTAGA 125 23 0 39.13 60.550 0.00 0.00 0.00 3.550 28 CGTGCTTTGCGTTTTGGA 127 18 0 50.00 58.175 0.00 0.00 0.00 3.825 29 GTGCTTTGCGTTTTGGAAG 126 19 0 47.37 57.116 0.00 0.00 0.00 3.884 30 GCGTGCTTTGCGTTTTGGAAG 128 21 0 52.38 62.910 0.00 0.00 0.00 3.910 31 GATCGGGAGCGTGCTTTGC 136 19 0 63.16 62.988 0.00 0.00 0.00 3.988 32 TGCGTTTTGGAAGTTAGACTTTG 120 23 0 39.13 59.001 0.00 0.00 39.76 3.999 33 ATCGGGAGCGTGCTTTGC 135 18 0 61.11 62.020 0.00 0.00 0.00 4.020 34 GCGTTTTGGAAGTTAGACTTTGAC 119 24 0 41.67 59.943 0.00 0.00 39.76 4.057 35 CGTGCTTTGCGTTTTGGAAGTT 127 22 0 45.45 62.204 0.00 0.00 0.00 4.204 36 GCGTTTTGGAAGTTAGACTTTGA 119 23 0 39.13 58.752 0.00 0.00 39.76 4.248 37 CTTTGCGTTTTGGAAGTTAGACT 123 23 0 39.13 58.749 0.00 0.00 0.00 4.251 38 TGCGTTTTGGAAGTTAGACTTTGA 120 24 0 37.50 60.297 0.00 0.00 39.76 4.297 39 TTTGCGTTTTGGAAGTTAGACT 122 22 0 36.36 57.684 0.00 0.00 0.00 4.316 40 TTGCGTTTTGGAAGTTAGACTT 121 22 0 36.36 57.684 0.00 0.00 42.60 4.316 41 TGCGTTTTGGAAGTTAGACTTT 120 22 0 36.36 57.684 0.00 0.00 39.76 4.316 42 TTGCGTTTTGGAAGTTAGACTTTG 121 24 0 37.50 59.584 0.00 0.00 39.76 4.416 43 GGGAGCGTGCTTTGCGTT 132 18 0 61.11 62.550 0.00 0.00 37.77 4.550 44 TTGACAACAGACTCATACAGTGA 100 23 0 39.13 58.405 0.00 0.00 35.23 4.595 45 CTTTGCGTTTTGGAAGTTAGAC 123 22 0 40.91 57.367 0.00 0.00 0.00 4.633 46 GCGTTTTGGAAGTTAGACTTTG 119 22 0 40.91 57.367 0.00 0.00 39.76 4.633 47 TTTGCGTTTTGGAAGTTAGACTT 122 23 0 34.78 58.357 0.00 0.00 42.60 4.643 48 TTGCGTTTTGGAAGTTAGACTTT 121 23 0 34.78 58.357 0.00 0.00 39.76 4.643 49 CTTTGCGTTTTGGAAGTTAGACTT 123 24 0 37.50 59.344 0.00 0.00 42.60 4.656 50 CGTGCTTTGCGTTTTGGAAGTTA 127 23 0 43.48 61.942 0.00 0.00 0.00 4.942 51 TTTGACAACAGACTCATACAGTGA 101 24 0 37.50 59.026 0.00 0.00 35.23 4.974 52 ACTTTGACAACAGACTCATACAGT 103 24 0 37.50 59.023 0.00 0.00 38.87 4.977 53 TTTGCGTTTTGGAAGTTAGACTTT 122 24 0 33.33 58.975 0.00 0.00 39.76 5.025 54 TGGAAGTTAGACTTTGACAACAGA 113 24 0 37.50 58.959 0.00 0.00 39.76 5.041 55 CTTTGACAACAGACTCATACAGTGA 102 25 0 40.00 59.948 0.00 0.00 35.23 5.052 56 GACTTTGACAACAGACTCATACAGT 104 25 0 40.00 59.948 0.00 0.00 38.87 5.052 57 CTTTGCGTTTTGGAAGTTAGACTTT 123 25 0 36.00 59.893 0.00 0.00 39.76 5.107 58 GGAAGTTAGACTTTGACAACAGACT 112 25 0 40.00 59.889 0.00 0.00 39.76 5.111 59 TGGAAGTTAGACTTTGACAACAGAC 113 25 0 40.00 60.121 0.00 0.00 39.76 5.121 60 TTTGCGTTTTGGAAGTTAGACTTTG 122 25 0 36.00 60.122 0.00 0.00 39.76 5.122 61 ACTTTGACAACAGACTCATACAGTG 103 25 0 40.00 60.179 0.00 0.00 35.23 5.179 62 CGTTTTGGAAGTTAGACTTTGACA 118 24 0 37.50 58.808 0.00 0.00 39.76 5.192 63 AGACTTTGACAACAGACTCATACA 105 24 0 37.50 58.779 0.00 0.00 0.00 5.221 64 AGTTAGACTTTGACAACAGACTCA 109 24 0 37.50 58.718 0.00 0.00 0.00 5.282 65 AGACTTTGACAACAGACTCATACAG 105 25 0 40.00 59.716 0.00 0.00 0.00 5.284 66 CTTTGACAACAGACTCATACAGTG 102 24 0 41.67 58.696 0.00 0.00 35.23 5.304 67 TTTGACAACAGACTCATACAGTG 101 23 0 39.13 57.679 0.00 0.00 35.23 5.321 68 GGAAGTTAGACTTTGACAACAGAC 112 24 0 41.67 58.631 0.00 0.00 39.76 5.369 69 TGGAAGTTAGACTTTGACAACAG 113 23 0 39.13 57.603 0.00 0.00 39.76 5.397 70 GCTTTGCGTTTTGGAAGTTAGACT 124 24 0 41.67 61.422 0.00 0.00 0.00 5.422 71 TTGGAAGTTAGACTTTGACAACAGA 114 25 0 36.00 59.535 0.00 0.00 39.76 5.465 72 GACTTTGACAACAGACTCATACAG 104 24 0 41.67 58.458 0.00 0.00 0.00 5.542 73 GACTTTGACAACAGACTCATACA 104 23 0 39.13 57.427 0.00 0.00 0.00 5.573 74 ACTTTGACAACAGACTCATACAG 103 23 0 39.13 57.421 0.00 0.00 0.00 5.579 75 CTTTGACAACAGACTCATACAGT 102 23 0 39.13 57.421 0.00 0.00 38.87 5.579 76 CGTTTTGGAAGTTAGACTTTGACAA 118 25 0 36.00 59.378 0.00 0.72 39.76 5.622 77 GTTAGACTTTGACAACAGACTCA 108 23 0 39.13 57.365 0.00 0.00 0.00 5.635 78 GGAAGTTAGACTTTGACAACAGA 112 23 0 39.13 57.349 0.00 0.00 39.76 5.651 79 GTGCTTTGCGTTTTGGAAGTTAGA 126 24 0 41.67 61.655 0.00 0.00 0.00 5.655 80 TGCTTTGCGTTTTGGAAGTTAGAC 125 24 0 41.67 61.655 0.00 0.00 0.00 5.655 81 AAGTTAGACTTTGACAACAGACTCA 110 25 0 36.00 59.303 0.00 0.00 35.26 5.697 82 TTGGAAGTTAGACTTTGACAACAG 114 24 0 37.50 58.252 0.00 0.00 39.76 5.748 83 CGTTTTGGAAGTTAGACTTTGAC 118 23 0 39.13 57.231 0.00 0.00 39.76 5.769 84 TTGGAAGTTAGACTTTGACAACA 114 23 0 34.78 57.194 0.00 0.00 39.76 5.806 85 TTGCGTTTTGGAAGTTAGACTTTGA 121 25 0 36.00 60.814 0.00 0.00 39.76 5.814 86 AGACTTTGACAACAGACTCATAC 105 23 0 39.13 57.168 0.00 0.00 0.00 5.832 87 AGTTAGACTTTGACAACAGACTCAT 109 25 0 36.00 59.125 0.00 0.00 0.00 5.875 88 AGTTAGACTTTGACAACAGACTC 109 23 0 39.13 57.106 0.00 0.00 0.00 5.894 89 TTTGGAAGTTAGACTTTGACAACAGA 115 26 0 34.62 60.068 0.00 0.00 39.76 6.068 90 TTTGGAAGTTAGACTTTGACAACA 115 24 0 33.33 57.867 0.00 0.00 39.76 6.133 91 TTTGGAAGTTAGACTTTGACAACAG 115 25 0 36.00 58.850 0.00 0.00 39.76 6.150 92 GTTAGACTTTGACAACAGACTCAT 108 24 0 37.50 57.834 0.00 0.00 0.00 6.166 93 GAAGTTAGACTTTGACAACAGACTCA 111 26 0 38.46 60.180 0.00 0.00 39.76 6.180 94 GAAGTTAGACTTTGACAACAGACT 111 24 0 37.50 57.774 0.00 0.00 39.76 6.226 95 AAGTTAGACTTTGACAACAGACTC 110 24 0 37.50 57.774 0.00 0.00 35.26 6.226 96 GAAGTTAGACTTTGACAACAGACTC 111 25 0 40.00 58.753 0.00 0.00 39.76 6.247 97 AAGTTAGACTTTGACAACAGACTCAT 110 26 0 34.62 59.673 0.00 0.00 35.26 6.327 98 TAGACTTTGACAACAGACTCATACA 106 25 0 36.00 58.659 0.00 0.00 0.00 6.341 99 GTTTTGGAAGTTAGACTTTGACAACA 117 26 0 34.62 59.625 0.00 0.00 39.76 6.375 100 TGCGTTTTGGAAGTTAGACTTTGAC 120 25 0 40.00 61.376 0.00 0.00 39.76 6.376 101 GCGTTTTGGAAGTTAGACTTTGACA 119 25 0 40.00 61.376 0.00 0.00 39.76 6.376 102 TAGACTTTGACAACAGACTCATACAG 106 26 0 38.46 59.568 0.00 0.00 0.00 6.432 103 CGTTTTGGAAGTTAGACTTTGACAAC 118 26 0 38.46 60.452 0.00 0.00 39.76 6.452 104 TTTTGGAAGTTAGACTTTGACAACA 116 25 0 32.00 58.486 0.00 0.00 39.76 6.514 105 TTTTGGAAGTTAGACTTTGACAACAG 116 26 0 34.62 59.402 0.00 0.00 39.76 6.598 106 TTGGAAGTTAGACTTTGACAACAGAC 114 26 0 38.46 60.625 0.00 0.00 39.76 6.625 107 CGTGCTTTGCGTTTTGGAAGTTAG 127 24 0 45.83 62.725 0.00 0.00 0.00 6.725 108 GGAAGTTAGACTTTGACAACAGACTC 112 26 0 42.31 60.731 0.00 0.00 39.76 6.731 109 TTAGACTTTGACAACAGACTCATACA 107 26 0 34.62 59.224 0.00 0.00 0.00 6.776 110 GTTTTGGAAGTTAGACTTTGACAAC 117 25 0 36.00 58.183 0.00 0.00 39.76 6.817 111 GCTTTGCGTTTTGGAAGTTAGACTT 124 25 0 40.00 61.888 0.00 0.00 42.60 6.888 112 TAGACTTTGACAACAGACTCATAC 106 24 0 37.50 57.108 0.00 0.00 0.00 6.892 113 CTTTGCGTTTTGGAAGTTAGACTTTG 123 26 0 38.46 60.941 0.00 0.00 39.76 6.941 114 GACTTTGACAACAGACTCATACAGTG 104 26 0 42.31 61.002 0.00 0.00 35.23 7.002 115 AGTTAGACTTTGACAACAGACTCATA 109 26 0 34.62 58.996 0.00 0.00 0.00 7.004 116 AGTTAGACTTTGACAACAGACTCATAC 109 27 0 37.04 60.074 0.00 0.00 0.00 7.074 117 TTAGACTTTGACAACAGACTCATACAG 107 27 0 37.04 60.074 0.00 0.00 0.00 7.074 118 GTTAGACTTTGACAACAGACTCATAC 108 26 0 38.46 58.916 0.00 0.00 0.00 7.084 119 AGACTTTGACAACAGACTCATACAGT 105 26 0 38.46 61.136 0.00 0.00 38.87 7.136 120 GTTAGACTTTGACAACAGACTCATA 108 25 0 36.00 57.751 0.00 0.00 0.00 7.249 121 TTAGACTTTGACAACAGACTCATAC 107 25 0 36.00 57.751 0.00 0.00 0.00 7.249 122 GTTAGACTTTGACAACAGACTCATACA 108 27 0 37.04 60.288 0.00 0.00 0.00 7.288 123 TTTGCGTTTTGGAAGTTAGACTTTGA 122 26 0 34.62 61.290 0.00 0.00 39.76 7.290 124 TGGAAGTTAGACTTTGACAACAGACT 113 26 0 38.46 61.307 0.00 0.00 39.76 7.307 125 ACTTTGACAACAGACTCATACAGTGA 103 26 0 38.46 61.357 0.00 0.00 35.23 7.357 126 GTTTTGGAAGTTAGACTTTGACAACAG 117 27 0 37.04 60.446 0.00 0.00 39.76 7.446 127 AAGTTAGACTTTGACAACAGACTCATA 110 27 0 33.33 59.530 0.00 0.00 35.26 7.470 128 GAAGTTAGACTTTGACAACAGACTCAT 111 27 0 37.04 60.503 0.00 0.00 39.76 7.503 129 TTTTGGAAGTTAGACTTTGACAACAGA 116 27 0 33.33 60.560 0.00 0.00 39.76 7.560 130 GTGCTTTGCGTTTTGGAAGTTAGAC 126 25 0 44.00 62.649 0.00 0.00 0.00 7.649 131 TTGCGTTTTGGAAGTTAGACTTTGAC 121 26 0 38.46 61.826 0.00 0.00 39.76 7.826 132 GCGTTTTGGAAGTTAGACTTTGACAA 119 26 0 38.46 61.826 0.00 0.72 39.76 7.826 133 TGCTTTGCGTTTTGGAAGTTAGACT 125 25 0 40.00 62.829 0.00 0.00 0.00 7.829 134 TAGACTTTGACAACAGACTCATACAGT 106 27 0 37.04 60.940 0.00 0.00 38.87 7.940 135 TTTGGAAGTTAGACTTTGACAACAGAC 115 27 0 37.04 61.092 0.00 0.00 39.76 8.092 136 GCTTTGCGTTTTGGAAGTTAGACTTT 124 26 0 38.46 62.319 0.00 0.00 39.76 8.319 137 TGCGTTTTGGAAGTTAGACTTTGACA 120 26 0 38.46 62.731 0.00 0.00 39.76 8.731 138 TTGGAAGTTAGACTTTGACAACAGACT 114 27 0 37.04 61.754 0.00 0.00 39.76 8.754 139 CGTTTTGGAAGTTAGACTTTGACAACA 118 27 0 37.04 61.772 0.00 0.00 39.76 8.772 140 CTTTGCGTTTTGGAAGTTAGACTTTGA 123 27 0 37.04 62.036 0.00 0.00 39.76 9.036 141 TGGAAGTTAGACTTTGACAACAGACTC 113 27 0 40.74 62.059 0.00 0.00 39.76 9.059 142 GGAAGTTAGACTTTGACAACAGACTCA 112 27 0 40.74 62.059 0.00 0.00 39.76 9.059 143 GACTTTGACAACAGACTCATACAGTGA 104 27 0 40.74 62.103 0.00 0.00 35.23 9.103 144 AGACTTTGACAACAGACTCATACAGTG 105 27 0 40.74 62.107 0.00 0.00 35.23 9.107 145 TTTGCGTTTTGGAAGTTAGACTTTGAC 122 27 0 37.04 62.244 0.00 0.00 39.76 9.244 146 GCGTTTTGGAAGTTAGACTTTGACAAC 119 27 0 40.74 62.744 0.00 0.00 39.76 9.744