ACCEPTABLE RIGHT PRIMERS 1-based # self self hair- qual- # sequence start ln N GC% Tm any_th end_th pin lity 0 TTTTTTAAACGACGGCCAGT 283 20 0 40.00 57.391 2.58 1.20 0.00 2.609 1 ATTTTTTAAACGACGGCCAGT 284 21 0 38.10 57.921 2.58 1.20 0.00 3.079 2 TTTTAAACGACGGCCAGTG 281 19 0 47.37 57.368 2.58 0.00 0.00 3.632 3 TTTTTAAACGACGGCCAGTG 282 20 0 45.00 58.136 2.58 0.00 0.00 1.864 4 TTTTTTAAACGACGGCCAGTG 283 21 0 42.86 58.833 2.58 0.00 0.00 2.167 5 ATTTTTTAAACGACGGCCAGTG 284 22 0 40.91 59.275 2.58 0.00 0.00 2.725 6 TTAAACGACGGCCAGTGA 279 18 0 50.00 57.405 2.58 0.00 0.00 4.595 7 TTTAAACGACGGCCAGTGA 280 19 0 47.37 58.222 2.58 0.00 0.00 2.778 8 TTTTAAACGACGGCCAGTGA 281 20 0 45.00 58.960 2.58 0.00 0.00 1.040 9 TTTTTAAACGACGGCCAGTGA 282 21 0 42.86 59.629 2.58 0.00 0.00 1.371 10 TTTTTTAAACGACGGCCAGTGA 283 22 0 40.91 60.239 2.58 0.00 0.00 2.239 11 ATTTTTTAAACGACGGCCAGTGA 284 23 0 39.13 60.612 2.58 0.00 0.00 3.612 12 TAAACGACGGCCAGTGAG 278 18 0 55.56 57.909 2.58 1.62 0.00 4.091 13 TTAAACGACGGCCAGTGAG 279 19 0 52.63 58.690 2.58 1.62 0.00 2.310 14 TTTAAACGACGGCCAGTGAG 280 20 0 50.00 59.397 2.58 1.62 0.00 0.603 15 TTTTAAACGACGGCCAGTGAG 281 21 0 47.62 60.038 2.58 1.62 0.00 1.038 16 TTTTTAAACGACGGCCAGTGAG 282 22 0 45.45 60.623 2.58 1.62 0.00 2.623 17 TTTTTTAAACGACGGCCAGTGAG 283 23 0 43.48 61.159 2.58 1.62 0.00 4.159 18 ATTTTTTAAACGACGGCCAGTGAG 284 24 0 41.67 61.479 2.58 1.62 0.00 5.479 19 AAACGACGGCCAGTGAGC 277 18 0 61.11 61.557 2.58 0.00 0.00 3.557 20 TAAACGACGGCCAGTGAGC 278 19 0 57.89 61.271 2.58 0.00 0.00 2.271 21 TTAAACGACGGCCAGTGAGC 279 20 0 55.00 61.851 2.58 0.00 0.00 1.851 22 TTTAAACGACGGCCAGTGAGC 280 21 0 52.38 62.377 2.58 0.00 0.00 3.377 23 TTTTAAACGACGGCCAGTGAGC 281 22 0 50.00 62.858 2.58 0.00 0.00 4.858 24 CCAGTGAGCGCGCGTAAT 268 18 0 61.11 61.764 32.80 18.47 0.00 3.764 25 CAGTGAGCGCGCGTAATA 267 18 0 55.56 58.877 32.80 13.29 0.00 3.123 26 CCAGTGAGCGCGCGTAATA 268 19 0 57.89 61.473 32.80 13.29 0.00 2.473 27 CCAGAGATCCACCGTCGG 228 18 0 66.67 60.088 10.98 10.98 0.00 2.088 28 CAGAGATCCACCGTCGGT 227 18 0 61.11 59.298 12.95 12.95 36.10 2.702 29 CCAGAGATCCACCGTCGGT 228 19 0 63.16 61.972 12.95 12.95 36.10 2.972 30 AGAGATCCACCGTCGGTA 226 18 0 55.56 57.512 19.07 3.03 32.96 4.488 31 CAGAGATCCACCGTCGGTA 227 19 0 57.89 59.119 19.07 3.03 32.96 1.881 32 CCAGAGATCCACCGTCGGTA 228 20 0 60.00 61.674 19.07 3.03 32.96 1.674