ACCEPTABLE LEFT PRIMERS 1-based # self self hair- qual- # sequence start ln N GC% Tm any_th end_th pin lity 0 AACCTGGCTACAGTTCCCTC 72 20 0 55.00 59.951 0.00 0.00 43.93 0.049 1 TATTCCTGTCCCTGTGCCTC 49 20 0 55.00 59.732 0.00 0.00 0.00 0.268 2 AAACCTGGCTACAGTTCCCT 71 20 0 50.00 59.503 0.00 0.00 43.93 0.497 3 CTGTCCCTGTGCCTCAGAAA 54 20 0 55.00 60.532 7.94 0.00 46.81 0.532 4 GCCTCAGAAACCTGGCTACA 64 20 0 55.00 60.603 0.00 0.00 42.38 0.603 5 TGCCTCAGAAACCTGGCTAC 63 20 0 55.00 60.603 0.00 0.00 45.57 0.603 6 GTGCCTCAGAAACCTGGCTA 62 20 0 55.00 60.603 0.00 0.00 45.57 0.603 7 TGTCCCTGTGCCTCAGAAAC 55 20 0 55.00 60.819 7.94 6.46 46.81 0.819 8 GAAACCTGGCTACAGTTCCC 70 20 0 55.00 59.095 0.00 0.00 43.93 0.905 9 ATATTCCTGTCCCTGTGCCT 48 20 0 50.00 59.053 0.00 0.00 0.00 0.947 10 ATTCCTGTCCCTGTGCCTC 50 19 0 57.89 59.938 0.00 0.00 0.00 1.062 11 CTGTCCCTGTGCCTCAGAA 54 19 0 57.89 59.863 7.94 0.00 46.81 1.137 12 CCCTGTGCCTCAGAAACCT 58 19 0 57.89 60.168 7.94 0.00 46.81 1.168 13 TCCCTGTGCCTCAGAAACC 57 19 0 57.89 60.168 7.94 0.00 46.81 1.168 14 ATATTCCTGTCCCTGTGCCTC 48 21 0 52.38 60.172 0.00 0.00 0.00 1.172 15 AATATTCCTGTCCCTGTGCCT 47 21 0 47.62 59.747 0.00 0.00 0.00 1.253 16 CCTCAGAAACCTGGCTACAGT 65 21 0 52.38 60.311 0.00 0.00 43.93 1.311 17 CCTCAGAAACCTGGCTACAG 65 20 0 55.00 58.522 0.00 0.00 44.78 1.478 18 CAGAAACCTGGCTACAGTTCC 68 21 0 52.38 59.491 0.00 0.00 43.93 1.509 19 GTCCCTGTGCCTCAGAAACC 56 20 0 60.00 61.528 7.94 0.00 46.81 1.528 20 CCTGTGCCTCAGAAACCTGG 59 20 0 60.00 61.531 7.94 0.00 46.81 1.531 21 CCCTGTGCCTCAGAAACCTG 58 20 0 60.00 61.531 7.94 0.00 46.81 1.531 22 AAACCTGGCTACAGTTCCCTC 71 21 0 52.38 60.589 0.00 0.00 43.93 1.589 23 GAAACCTGGCTACAGTTCCCT 70 21 0 52.38 60.589 0.00 0.00 43.93 1.589 24 AGAAACCTGGCTACAGTTCCC 69 21 0 52.38 60.589 0.00 0.00 43.93 1.589 25 TGTCCCTGTGCCTCAGAAA 55 19 0 52.63 59.397 7.94 0.00 46.81 1.603 26 TCCCTGTGCCTCAGAAACCT 57 20 0 55.00 61.721 7.94 0.00 46.81 1.721 27 ACCTGGCTACAGTTCCCTC 73 19 0 57.89 59.246 0.00 0.00 43.93 1.754 28 ATTCCTGTCCCTGTGCCTCA 50 20 0 55.00 61.795 0.00 0.00 0.00 1.795 29 AATATTCCTGTCCCTGTGCC 47 20 0 50.00 58.196 0.00 0.00 0.00 1.804 30 TGCCTCAGAAACCTGGCTA 63 19 0 52.63 59.164 0.00 0.00 45.57 1.836 31 GTGCCTCAGAAACCTGGCT 62 19 0 57.89 60.850 0.00 0.00 45.57 1.850 32 AGAAACCTGGCTACAGTTCC 69 20 0 50.00 58.065 0.00 0.00 43.93 1.935 33 GTCCCTGTGCCTCAGAAAC 56 19 0 57.89 58.972 7.94 3.11 46.81 2.028 34 CTGTGCCTCAGAAACCTGG 60 19 0 57.89 58.969 0.00 0.00 46.81 2.031 35 CCTGTGCCTCAGAAACCTG 59 19 0 57.89 58.969 7.94 0.00 46.81 2.031 36 TGTGCCTCAGAAACCTGGC 61 19 0 57.89 61.149 0.00 0.00 45.34 2.149 37 CTGTGCCTCAGAAACCTGGC 60 20 0 60.00 62.156 0.00 0.00 46.81 2.156 38 CTCAGAAACCTGGCTACAGT 66 20 0 50.00 57.783 0.00 0.00 43.93 2.217 39 AACCTGGCTACAGTTCCCT 72 19 0 52.63 58.763 0.00 0.00 43.93 2.237 40 CCTGTCCCTGTGCCTCAG 53 18 0 66.67 60.245 0.33 0.33 43.51 2.245 41 GCCTCAGAAACCTGGCTAC 64 19 0 57.89 58.746 0.00 0.00 42.38 2.254 42 TTCCTGTCCCTGTGCCTCA 51 19 0 57.89 61.407 0.00 0.00 0.00 2.407 43 CTCAGAAACCTGGCTACAGTTC 66 22 0 50.00 59.590 0.00 0.00 43.93 2.410 44 CCTGTCCCTGTGCCTCAGAA 53 20 0 60.00 62.423 7.94 0.00 46.81 2.423 45 TTCCTGTCCCTGTGCCTCAG 51 20 0 60.00 62.423 0.33 0.33 43.51 2.423 46 TAATATTCCTGTCCCTGTGCCT 46 22 0 45.45 59.565 0.00 0.00 0.00 2.435 47 TATTCCTGTCCCTGTGCCT 49 19 0 52.63 58.528 0.00 0.00 0.00 2.472 48 TCAGAAACCTGGCTACAGTTC 67 21 0 47.62 58.522 0.00 0.00 43.93 2.478 49 CTCAGAAACCTGGCTACAGTT 66 21 0 47.62 58.518 0.00 0.00 43.93 2.482 50 TATTCCTGTCCCTGTGCCTCA 49 21 0 52.38 61.511 0.00 0.00 0.00 2.511 51 TTCCTGTCCCTGTGCCTC 51 18 0 61.11 59.440 0.00 0.00 0.00 2.560 52 GCCTCAGAAACCTGGCTACAG 64 21 0 57.14 61.600 0.00 0.00 44.78 2.600 53 TGCCTCAGAAACCTGGCT 63 18 0 55.56 59.352 0.00 0.00 45.57 2.648 54 TGTGCCTCAGAAACCTGGCT 61 20 0 55.00 62.654 0.00 0.00 45.57 2.654 55 TCAGAAACCTGGCTACAGTT 67 20 0 45.00 57.313 0.00 0.00 43.93 2.687 56 TCCTGTCCCTGTGCCTCA 52 18 0 61.11 60.738 0.00 0.00 0.00 2.738 57 AATATTCCTGTCCCTGTGCCTC 47 22 0 50.00 60.773 0.00 0.00 0.00 2.773 58 GTGCCTCAGAAACCTGGC 62 18 0 61.11 59.225 0.00 0.00 45.34 2.775 59 CTGTCCCTGTGCCTCAGAAAC 54 21 0 57.14 61.802 7.94 6.46 46.81 2.802 60 CCTGTCCCTGTGCCTCAGA 53 19 0 63.16 61.850 7.94 0.00 46.81 2.850 61 TCCTGTCCCTGTGCCTCAG 52 19 0 63.16 61.850 0.33 0.33 43.51 2.850 62 GTGCCTCAGAAACCTGGCTAC 62 21 0 57.14 61.867 0.00 0.00 45.57 2.867 63 CTGTCCCTGTGCCTCAGA 54 18 0 61.11 59.122 7.94 0.00 46.81 2.878 64 TCAGAAACCTGGCTACAGTTCC 67 22 0 50.00 60.900 0.00 0.00 43.93 2.900 65 CCTCAGAAACCTGGCTACAGTT 65 22 0 50.00 60.901 0.00 0.00 43.93 2.901 66 TAATATTCCTGTCCCTGTGCC 46 21 0 47.62 58.080 0.00 0.00 0.00 2.920 67 AAACCTGGCTACAGTTCCC 71 19 0 52.63 57.870 0.00 0.00 43.93 3.130 68 TTAATATTCCTGTCCCTGTGCCT 45 23 0 43.48 60.175 0.00 0.00 0.00 3.175 69 TTAATATTCCTGTCCCTGTGCC 45 22 0 45.45 58.776 0.00 0.00 0.00 3.224 70 ATTCCTGTCCCTGTGCCT 50 18 0 55.56 58.683 0.00 0.00 0.00 3.317 71 TGCCTCAGAAACCTGGCTACA 63 21 0 52.38 62.333 0.00 0.00 45.57 3.333 72 TGTGCCTCAGAAACCTGGCTA 61 21 0 52.38 62.333 0.00 0.00 45.57 3.333 73 TGTCCCTGTGCCTCAGAA 55 18 0 55.56 58.619 7.94 0.00 46.81 3.381 74 CCCTGTGCCTCAGAAACC 58 18 0 61.11 58.501 7.94 0.00 46.81 3.499 75 GAAACCTGGCTACAGTTCCCTC 70 22 0 54.55 61.548 0.00 0.00 43.93 3.548 76 TAATATTCCTGTCCCTGTGCCTC 46 23 0 47.83 60.559 0.00 0.00 0.00 3.559 77 ATATTCCTGTCCCTGTGCC 48 19 0 52.63 57.398 0.00 0.00 0.00 3.602 78 CCTCAGAAACCTGGCTACA 65 19 0 52.63 57.267 0.00 0.00 33.11 3.733 79 ATTCCTGTCCCTGTGCCTCAG 50 21 0 57.14 62.743 0.33 0.33 43.51 3.743 80 ATTAATATTCCTGTCCCTGTGCC 44 23 0 43.48 59.217 0.00 0.00 0.00 3.783 81 CAGAAACCTGGCTACAGTTCCC 68 22 0 54.55 61.808 0.00 0.00 43.93 3.808 82 ATATTCCTGTCCCTGTGCCTCA 48 22 0 50.00 61.861 0.00 0.00 0.00 3.861 83 TCCCTGTGCCTCAGAAACCTG 57 21 0 57.14 62.942 7.94 0.00 46.81 3.942 84 CCTGTCCCTGTGCCTCAGAAA 53 21 0 57.14 62.942 7.94 0.00 46.81 3.942 85 GTCCCTGTGCCTCAGAAACCT 56 21 0 57.14 62.943 7.94 0.00 46.81 3.943 86 AGAAACCTGGCTACAGTTCCCT 69 22 0 50.00 61.994 0.00 0.00 43.93 3.994 87 ACCTGGCTACAGTTCCCT 73 18 0 55.56 57.939 0.00 0.00 43.93 4.061 88 TGTGCCTCAGAAACCTGG 61 18 0 55.56 57.684 0.00 0.00 33.11 4.316 89 TATTCCTGTCCCTGTGCCTCAG 49 22 0 54.55 62.434 0.33 0.33 43.51 4.434 90 ATTAATATTCCTGTCCCTGTGCCT 44 24 0 41.67 60.550 0.00 0.00 0.00 4.550 91 TCCCTGTGCCTCAGAAAC 57 18 0 55.56 57.361 7.94 0.00 46.81 4.639 92 GTCCCTGTGCCTCAGAAA 56 18 0 55.56 57.361 7.94 0.00 46.81 4.639 93 CCTGTGCCTCAGAAACCT 59 18 0 55.56 57.353 7.94 0.00 46.81 4.647 94 CCTGGCTACAGTTCCCTC 74 18 0 61.11 57.215 0.00 0.00 43.93 4.785 95 CTCAGAAACCTGGCTACAGTTCC 66 23 0 52.17 61.799 0.00 0.00 43.93 4.799 96 CCTCAGAAACCTGGCTACAGTTC 65 23 0 52.17 61.799 0.00 0.00 43.93 4.799 97 GCCTCAGAAACCTGGCTA 64 18 0 55.56 57.116 0.00 0.00 42.38 4.884 98 TATTAATATTCCTGTCCCTGTGCC 43 24 0 41.67 59.073 0.00 0.00 0.00 4.927 99 AACCTGGCTACAGTTCCC 72 18 0 55.56 57.014 0.00 0.00 43.93 4.986 100 TTAATATTCCTGTCCCTGTGCCTC 45 24 0 45.83 61.094 0.00 0.00 0.00 5.094 101 TATTAATATTCCTGTCCCTGTGCCT 43 25 0 40.00 60.358 0.00 0.00 0.00 5.358 102 AATATTCCTGTCCCTGTGCCTCA 47 23 0 47.83 62.368 0.00 0.00 0.00 5.368 103 ATATTAATATTCCTGTCCCTGTGCC 42 25 0 40.00 59.470 2.66 0.00 0.00 5.530 104 ATATTCCTGTCCCTGTGCCTCAG 48 23 0 52.17 62.727 0.33 0.33 43.51 5.727 105 AGAAACCTGGCTACAGTTCCCTC 69 23 0 52.17 62.847 0.00 0.00 43.93 5.847 106 AATATTAATATTCCTGTCCCTGTGCC 41 26 0 38.46 60.010 14.65 0.00 0.00 6.010 107 TAATATTCCTGTCCCTGTGCCTCA 46 24 0 45.83 62.095 0.00 0.00 0.00 6.095 108 ATTAATATTCCTGTCCCTGTGCCTC 44 25 0 44.00 61.415 0.00 0.00 0.00 6.415 109 ATATTAATATTCCTGTCCCTGTGCCT 42 26 0 38.46 60.698 2.66 0.00 0.00 6.698 110 ATATTAATATTCCTGTCCCTGTGC 42 24 0 37.50 57.298 2.66 0.00 0.00 6.702 111 AATATTAATATTCCTGTCCCTGTGC 41 25 0 36.00 57.944 14.65 0.00 0.00 7.056 112 TATTAATATTCCTGTCCCTGTGCCTC 43 26 0 42.31 61.200 0.00 0.00 0.00 7.200 113 CAAATATTAATATTCCTGTCCCTGTGC 39 27 0 37.04 59.644 19.49 0.00 32.85 7.356 114 AAATATTAATATTCCTGTCCCTGTGC 40 26 0 34.62 58.541 19.49 0.00 32.85 7.459 115 AAATATTAATATTCCTGTCCCTGTGCC 40 27 0 37.04 60.509 19.49 0.00 32.85 7.509 116 TTAATATTCCTGTCCCTGTGCCTCA 45 25 0 44.00 62.553 0.00 0.00 0.00 7.553 117 TAATATTCCTGTCCCTGTGCCTCAG 46 25 0 48.00 62.882 0.00 0.33 43.51 7.882 118 AATATTAATATTCCTGTCCCTGTGCCT 41 27 0 37.04 61.178 14.65 0.00 0.00 8.178 119 ATATTAATATTCCTGTCCCTGTGCCTC 42 27 0 40.74 61.495 2.66 0.00 0.00 8.495 120 TCAAATATTAATATTCCTGTCCCTGTG 38 27 0 33.33 58.380 19.49 11.40 32.85 8.620 121 TCAAATATTAATATTCCTGTCCCTGT 38 26 0 30.77 57.195 19.49 0.53 32.85 8.805 122 ATTAATATTCCTGTCCCTGTGCCTCA 44 26 0 42.31 62.812 0.00 0.00 0.00 8.812 123 CTCAAATATTAATATTCCTGTCCCTGT 37 27 0 33.33 58.156 19.49 0.53 32.85 8.844 124 CAAATATTAATATTCCTGTCCCTGTG 39 26 0 34.62 57.154 19.49 8.47 32.85 8.846 125 TATTAATATTCCTGTCCCTGTGCCTCA 43 27 0 40.74 62.553 0.00 0.00 0.00 9.553