ACCEPTABLE RIGHT PRIMERS 1-based # self self hair- qual- # sequence start ln N GC% Tm any_th end_th pin lity 0 CAGTGCTCTTTCTGCCAGTG 176 20 0 55.00 60.030 0.00 0.00 0.00 0.030 1 GTGCTCTTTCTGCCAGTGTC 174 20 0 55.00 60.030 0.00 0.00 0.00 0.030 2 GCTCTTTCTGCCAGTGTCAC 172 20 0 55.00 60.030 0.00 0.00 0.00 0.030 3 CATCCAGTGCTCTTTCTGCC 180 20 0 55.00 59.816 0.00 0.00 0.00 0.184 4 ATCCAGTGCTCTTTCTGCCA 179 20 0 50.00 60.243 0.00 0.00 0.00 0.243 5 TGCTCTTTCTGCCAGTGTCA 173 20 0 50.00 60.459 0.00 0.00 0.00 0.459 6 AGTGCTCTTTCTGCCAGTGT 175 20 0 50.00 60.460 0.00 0.00 0.00 0.460 7 GATCCCTCATCCAGTGCTCT 187 20 0 55.00 59.513 0.00 0.00 0.00 0.487 8 TCCCTCATCCAGTGCTCTTT 185 20 0 50.00 59.284 0.00 0.00 0.00 0.716 9 TCCAGTGCTCTTTCTGCCAG 178 20 0 55.00 60.888 0.00 0.00 0.00 0.888 10 ATCCCTCATCCAGTGCTCTT 186 20 0 50.00 59.058 0.00 0.00 0.00 0.942 11 CTCATCCAGTGCTCTTTCTGC 182 21 0 52.38 59.903 0.00 0.00 0.00 1.097 12 CCCTCATCCAGTGCTCTTTC 184 20 0 55.00 58.881 0.00 0.00 0.00 1.119 13 GATCCCTCATCCAGTGCTCTT 187 21 0 52.38 60.172 0.00 0.00 0.00 1.172 14 CCAGTGCTCTTTCTGCCAGT 177 20 0 55.00 61.175 0.00 0.00 0.00 1.175 15 TCATCCAGTGCTCTTTCTGC 181 20 0 50.00 58.810 0.00 0.00 0.00 1.190 16 TCCAGTGCTCTTTCTGCCA 178 19 0 52.63 59.785 0.00 0.00 0.00 1.215 17 ATCCCTCATCCAGTGCTCTTT 186 21 0 47.62 59.749 0.00 0.00 0.00 1.251 18 TCCCTCATCCAGTGCTCTTTC 185 21 0 52.38 60.380 0.00 0.00 0.00 1.380 19 CCCTCATCCAGTGCTCTTTCT 184 21 0 52.38 60.380 0.00 0.00 0.00 1.380 20 TTGGCCCTCTGGTTAACCG 151 19 0 57.89 60.547 20.21 14.57 39.80 1.547 21 CCAGTGCTCTTTCTGCCAG 177 19 0 57.89 59.350 0.00 0.00 0.00 1.650 22 CCTCATCCAGTGCTCTTTCTG 183 21 0 52.38 59.287 0.00 0.00 0.00 1.713 23 GTGCTCTTTCTGCCAGTGT 174 19 0 52.63 58.895 0.00 0.00 0.00 2.105 24 CCTCATCCAGTGCTCTTTCT 183 20 0 50.00 57.849 0.00 0.00 0.00 2.151 25 TGGCCCTCTGGTTAACCG 150 18 0 61.11 59.845 20.21 14.57 39.80 2.155 26 GGCCCTCTGGTTAACCGT 149 18 0 61.11 59.845 20.21 0.00 39.80 2.155 27 TCATCCAGTGCTCTTTCTGCC 181 21 0 52.38 61.269 0.00 0.00 0.00 2.269 28 ATCCAGTGCTCTTTCTGCCAG 179 21 0 52.38 61.272 0.00 0.00 0.00 2.272 29 GGCCCTCTGGTTAACCGTG 149 19 0 63.16 61.289 20.21 15.09 39.80 2.289 30 TTGGCCCTCTGGTTAACCGT 151 20 0 55.00 62.367 20.21 0.00 39.80 2.367 31 TGCTCTTTCTGCCAGTGTC 173 19 0 52.63 58.595 0.00 0.00 0.00 2.405 32 GCTCTTTCTGCCAGTGTCA 172 19 0 52.63 58.595 0.00 0.00 0.00 2.405 33 CAGTGCTCTTTCTGCCAGT 176 19 0 52.63 58.590 0.00 0.00 0.00 2.410 34 AGTGCTCTTTCTGCCAGTG 175 19 0 52.63 58.590 0.00 0.00 0.00 2.410 35 TCCCTCATCCAGTGCTCTT 185 19 0 52.63 58.535 0.00 0.00 0.00 2.465 36 AGTGCTCTTTCTGCCAGTGTC 175 21 0 52.38 61.466 0.00 0.00 0.00 2.466 37 CATCCAGTGCTCTTTCTGCCA 180 21 0 52.38 61.542 0.00 0.00 0.00 2.542 38 ATCCAGTGCTCTTTCTGCC 179 19 0 52.63 58.350 0.00 0.00 0.00 2.650 39 ATCCCTCATCCAGTGCTCT 186 19 0 52.63 58.293 0.00 0.00 0.00 2.707 40 GTGCTCTTTCTGCCAGTGTCA 174 21 0 52.38 61.733 0.00 0.00 0.00 2.733 41 TGCTCTTTCTGCCAGTGTCAC 173 21 0 52.38 61.733 0.00 0.00 0.00 2.733 42 CAGTGCTCTTTCTGCCAGTGT 176 21 0 52.38 61.737 0.00 0.00 0.00 2.737 43 GATCCCTCATCCAGTGCTCTTT 187 22 0 50.00 60.771 0.00 0.00 0.00 2.771 44 ATCCCTCATCCAGTGCTCTTTC 186 22 0 50.00 60.771 0.00 0.00 0.00 2.771 45 TGGCCCTCTGGTTAACCGT 150 19 0 57.89 61.787 20.21 0.00 39.80 2.787 46 GATCCCTCATCCAGTGCTC 187 19 0 57.89 57.905 0.00 0.00 0.00 3.095 47 CCCTCATCCAGTGCTCTTT 184 19 0 52.63 57.642 0.00 0.00 0.00 3.358 48 CCAGTGCTCTTTCTGCCAGTG 177 21 0 57.14 62.397 0.00 0.00 0.00 3.397 49 GCCCTCTGGTTAACCGTG 148 18 0 61.11 58.599 20.21 15.09 39.80 3.401 50 TCCAGTGCTCTTTCTGCCAGT 178 21 0 52.38 62.602 0.00 0.00 0.00 3.602 51 CCCTCATCCAGTGCTCTTTCTG 184 22 0 54.55 61.611 0.00 0.00 0.00 3.611 52 TCCCTCATCCAGTGCTCTTTCT 185 22 0 50.00 61.787 0.00 0.00 0.00 3.787 53 CATCCAGTGCTCTTTCTGC 180 19 0 52.63 57.204 0.00 0.00 0.00 3.796 54 CCAGTGCTCTTTCTGCCA 177 18 0 55.56 58.100 0.00 0.00 0.00 3.900 55 CCTCATCCAGTGCTCTTTCTGC 183 22 0 54.55 62.182 0.00 0.00 0.00 4.182 56 CTCATCCAGTGCTCTTTCTGCC 182 22 0 54.55 62.182 0.00 0.00 0.00 4.182 57 TCCAGTGCTCTTTCTGCC 178 18 0 55.56 57.779 0.00 0.00 0.00 4.221 58 TCCCTCATCCAGTGCTCT 185 18 0 55.56 57.701 0.00 0.00 0.00 4.299 59 CATCCAGTGCTCTTTCTGCCAG 180 22 0 54.55 62.439 0.00 0.00 0.00 4.439 60 CAGTGCTCTTTCTGCCAGTGTC 176 22 0 54.55 62.611 0.00 0.00 0.00 4.611 61 TTGGCCCTCTGGTTAACC 151 18 0 55.56 57.328 18.88 18.88 0.00 4.672 62 GATCCCTCATCCAGTGCTCTTTC 187 23 0 52.17 61.680 0.00 0.00 0.00 4.680 63 TGCTCTTTCTGCCAGTGT 173 18 0 50.00 57.275 0.00 0.00 0.00 4.725 64 GTGCTCTTTCTGCCAGTGTCAC 174 22 0 54.55 62.861 0.00 0.00 0.00 4.861 65 TCATCCAGTGCTCTTTCTGCCA 181 22 0 50.00 62.893 0.00 0.00 0.00 4.893 66 ATCCAGTGCTCTTTCTGCCAGT 179 22 0 50.00 62.901 0.00 0.00 0.00 4.901 67 ATCCCTCATCCAGTGCTCTTTCT 186 23 0 47.83 62.110 0.00 0.00 0.00 5.110 68 TCCCTCATCCAGTGCTCTTTCTG 185 23 0 52.17 62.900 0.00 0.00 0.00 5.900 69 GATCCCTCATCCAGTGCTCTTTCT 187 24 0 50.00 62.921 0.00 0.00 0.00 6.921