ACCEPTABLE LEFT PRIMERS 0-based # self self qual- # sequence start ln N GC% Tm any end lity 0 TTAGAAGGGGTGTCTGGGTG 72 20 0 55.00 59.959 3.00 0.00 0.041 1 CTGCAGGTTTAGAAGGGGTG 64 20 0 55.00 59.728 6.00 0.00 0.272 2 CACCCTGCAGGTTTAGAAGG 60 20 0 55.00 59.728 8.00 0.00 0.272 3 AGCACACCCTGCAGGTTTAG 56 20 0 55.00 60.312 8.00 6.00 0.312 4 TGCAGGTTTAGAAGGGGTGT 65 20 0 50.00 59.592 4.00 0.00 0.408 5 CCTGCAGGTTTAGAAGGGGT 63 20 0 55.00 60.488 8.00 0.00 0.488 6 ACCCTGCAGGTTTAGAAGGG 61 20 0 55.00 60.488 8.00 2.00 0.488 7 CACCCTGTAAACCCTGGCT 7 19 0 57.89 59.974 3.00 3.00 1.026 8 TCTGCACCCTGTAAACCCTG 3 20 0 55.00 61.084 4.00 1.00 1.084 9 CACACCCTGCAGGTTTAGAAG 58 21 0 52.38 59.782 8.00 2.00 1.218 10 CACACCCTGCAGGTTTAGAA 58 20 0 50.00 58.773 8.00 2.00 1.227 11 GCACACCCTGCAGGTTTAGA 57 20 0 55.00 61.236 8.00 4.00 1.236 12 CACTGAGGTCAGCACACCC 46 19 0 63.16 60.311 6.00 2.00 1.311 13 CCACTGAGGTCAGCACACC 45 19 0 63.16 60.311 6.00 4.00 1.311 14 CCCACTGAGGTCAGCACAC 44 19 0 63.16 60.311 6.00 2.00 1.311 15 CCTGCAGGTTTAGAAGGGG 63 19 0 57.89 59.673 8.00 0.00 1.327 16 CCCTGCAGGTTTAGAAGGG 62 19 0 57.89 59.673 8.00 2.00 1.327 17 GCAGGTTTAGAAGGGGTGTC 66 20 0 55.00 58.646 2.00 1.00 1.354 18 ACTGAGGTCAGCACACCCTG 47 20 0 60.00 61.355 6.00 2.00 1.355 19 CACTGAGGTCAGCACACCCT 46 20 0 60.00 61.355 6.00 2.00 1.355 20 GCAGGTTTAGAAGGGGTGTCT 66 21 0 52.38 59.630 3.00 0.00 1.370 21 CTGAGGTCAGCACACCCTG 48 19 0 63.16 60.461 6.00 2.00 1.461 22 ATCTGCACCCTGTAAACCCT 2 20 0 50.00 58.525 4.00 0.00 1.475 23 TTTAGAAGGGGTGTCTGGGT 71 20 0 50.00 58.498 3.00 0.00 1.502 24 GTTTAGAAGGGGTGTCTGGG 70 20 0 55.00 58.498 3.00 0.00 1.502 25 GGTTTAGAAGGGGTGTCTGG 69 20 0 55.00 58.498 3.00 0.00 1.502 26 AGGTTTAGAAGGGGTGTCTGG 68 21 0 52.38 59.488 3.00 0.00 1.512 27 CTGCAGGTTTAGAAGGGGTGT 64 21 0 52.38 60.534 6.00 0.00 1.534 28 ACACCCTGCAGGTTTAGAAGG 59 21 0 52.38 60.534 8.00 0.00 1.534 29 GTTTAGAAGGGGTGTCTGGGT 70 21 0 52.38 59.355 3.00 0.00 1.645 30 GCACACCCTGCAGGTTTAG 57 19 0 57.89 59.303 8.00 6.00 1.697 31 AGCACACCCTGCAGGTTTA 56 19 0 52.63 59.303 8.00 4.00 1.697 32 CACCCTGTAAACCCTGGCTC 7 20 0 60.00 61.826 3.00 1.00 1.826 33 CCTGTAAACCCTGGCTCCT 10 19 0 57.89 59.148 3.00 0.00 1.852 34 CTGCACCCTGTAAACCCTG 4 19 0 57.89 59.146 4.00 1.00 1.854 35 CCTGTAAACCCTGGCTCCTG 10 20 0 60.00 61.944 3.00 2.00 1.944 36 GACACCCCACTGAGGTCAG 39 19 0 63.16 59.044 7.00 6.00 1.956 37 ACCCTGTAAACCCTGGCTC 8 19 0 57.89 59.003 3.00 1.00 1.997 38 CTGTAAACCCTGGCTCCTGC 11 20 0 60.00 62.090 3.00 2.00 2.090 39 ACCCCACTGAGGTCAGCAC 42 19 0 63.16 61.171 7.00 1.00 2.171 40 ACACCCCACTGAGGTCAGC 40 19 0 63.16 61.171 7.00 4.00 2.171 41 TGTAAACCCTGGCTCCTGC 12 19 0 57.89 61.192 3.00 2.00 2.192 42 CAGCACACCCTGCAGGTTTA 55 20 0 55.00 62.212 8.00 4.00 2.212 43 CCCCACTGAGGTCAGCAC 43 18 0 66.67 60.252 6.00 1.00 2.252 44 ACCCCACTGAGGTCAGCA 42 18 0 61.11 60.252 7.00 2.00 2.252 45 CACCCCACTGAGGTCAGC 41 18 0 66.67 60.252 7.00 4.00 2.252 46 TTTAGAAGGGGTGTCTGGGTG 71 21 0 52.38 61.267 3.00 0.00 2.267 47 TGCAGGTTTAGAAGGGGTG 65 19 0 52.63 58.707 4.00 0.00 2.293 48 CAGGTTTAGAAGGGGTGTCTG 67 21 0 52.38 58.705 3.00 1.00 2.295 49 ATCTGCACCCTGTAAACCCTG 2 21 0 52.38 61.297 4.00 1.00 2.297 50 TAGAAGGGGTGTCTGGGTGC 73 20 0 60.00 62.397 3.00 2.00 2.397 51 TGCAGGTTTAGAAGGGGTGTC 65 21 0 52.38 61.411 4.00 1.00 2.411 52 GAGGTCAGCACACCCTGC 50 18 0 66.67 60.425 4.00 2.00 2.425 53 TAGAAGGGGTGTCTGGGTG 73 19 0 57.89 58.495 3.00 0.00 2.505 54 ACCCTGTAAACCCTGGCTCC 8 20 0 60.00 62.523 3.00 2.00 2.523 55 CCCTGTAAACCCTGGCTCCT 9 20 0 60.00 62.632 3.00 0.00 2.632 56 TGAGGTCAGCACACCCTG 49 18 0 61.11 59.315 4.00 1.00 2.685 57 CCCACTGAGGTCAGCACA 44 18 0 61.11 59.315 6.00 3.00 2.685 58 CTGTAAACCCTGGCTCCTG 11 19 0 57.89 58.300 3.00 2.00 2.700 59 AGCACACCCTGCAGGTTT 56 18 0 55.56 59.261 8.00 6.00 2.739 60 CTGCACCCTGTAAACCCTGG 4 20 0 60.00 62.773 4.00 3.00 2.773 61 GTAAACCCTGGCTCCTGCCT 13 20 0 60.00 62.773 3.00 0.00 2.773 62 ACTGAGGTCAGCACACCCT 47 19 0 57.89 58.217 6.00 2.00 2.783 63 CCCTGTAAACCCTGGCTCC 9 19 0 63.16 61.789 3.00 2.00 2.789 64 GGGACACCCCACTGAGGT 37 18 0 66.67 60.812 7.00 7.00 2.812 65 TCTGCACCCTGTAAACCCT 3 19 0 52.63 58.144 4.00 0.00 2.856 66 TGCACCCTGTAAACCCTGG 5 19 0 57.89 61.900 4.00 3.00 2.900 67 CAGCACACCCTGCAGGTT 55 18 0 61.11 60.905 8.00 8.00 2.905 68 GTAAACCCTGGCTCCTGCC 13 19 0 63.16 61.937 3.00 2.00 2.937 69 GGTTTAGAAGGGGTGTCTGGG 69 21 0 57.14 61.965 3.00 0.00 2.965 70 GGACACCCCACTGAGGTCAG 38 20 0 65.00 62.981 7.00 6.00 2.981 71 GGACACCCCACTGAGGTCA 38 19 0 63.16 62.043 7.00 4.00 3.043 72 TAAACCCTGGCTCCTGCCT 14 19 0 57.89 62.055 3.00 0.00 3.055 73 CACCCTGTAAACCCTGGC 7 18 0 61.11 58.930 3.00 2.00 3.070 74 GCACCCTGTAAACCCTGG 6 18 0 61.11 58.930 3.00 3.00 3.070 75 AGCACACCCTGCAGGTTTAGA 56 21 0 52.38 62.124 8.00 4.00 3.124 76 TAAACCCTGGCTCCTGCC 14 18 0 61.11 61.147 3.00 2.00 3.147 77 GGACACCCCACTGAGGTC 38 18 0 66.67 58.806 7.00 5.00 3.194 78 CAGCACACCCTGCAGGTTT 55 19 0 57.89 62.292 8.00 6.00 3.292 79 GTCAGCACACCCTGCAGGT 53 19 0 63.16 62.397 8.00 6.00 3.397 80 TTTAGAAGGGGTGTCTGGG 71 19 0 52.63 57.569 3.00 0.00 3.431 81 GCACACCCTGCAGGTTTAGAA 57 21 0 52.38 62.477 8.00 2.00 3.477 82 AGAAGGGGTGTCTGGGTGC 74 19 0 63.16 62.484 3.00 2.00 3.484 83 AGGTCAGCACACCCTGCAG 51 19 0 63.16 62.524 6.00 6.00 3.524 84 TCAGCACACCCTGCAGGT 54 18 0 61.11 61.534 8.00 6.00 3.534 85 GTCAGCACACCCTGCAGG 53 18 0 66.67 61.534 8.00 8.00 3.534 86 GGTCAGCACACCCTGCAG 52 18 0 66.67 61.534 6.00 6.00 3.534 87 AGGTCAGCACACCCTGCA 51 18 0 61.11 61.534 4.00 4.00 3.534 88 GAAGGGGTGTCTGGGTGC 75 18 0 66.67 61.536 2.00 2.00 3.536 89 ATCTGCACCCTGTAAACCC 2 19 0 52.63 57.435 4.00 0.00 3.565 90 AGAAGGGGTGTCTGGGTG 74 18 0 61.11 58.416 3.00 0.00 3.584 91 GTAAACCCTGGCTCCTGC 13 18 0 61.11 58.230 3.00 2.00 3.770 92 CACACCCTGCAGGTTTAGA 58 19 0 52.63 57.225 8.00 3.00 3.775 93 GCACCCTGTAAACCCTGGC 6 19 0 63.16 62.779 3.00 2.00 3.779 94 GCACACCCTGCAGGTTTA 57 18 0 55.56 58.186 8.00 4.00 3.814 95 GGGACACCCCACTGAGGTC 37 19 0 68.42 62.829 7.00 5.00 3.829 96 TCAGCACACCCTGCAGGTT 54 19 0 57.89 62.920 8.00 8.00 3.920 97 CCTGTAAACCCTGGCTCC 10 18 0 61.11 58.071 3.00 2.00 3.929 98 CCCTGTAAACCCTGGCTC 9 18 0 61.11 58.071 3.00 1.00 3.929 99 TGCACCCTGTAAACCCTG 5 18 0 55.56 58.020 4.00 1.00 3.980 100 GTTTAGAAGGGGTGTCTGGGTG 70 22 0 54.55 61.999 3.00 0.00 3.999 101 CAGGTTTAGAAGGGGTGTCTGG 67 22 0 54.55 62.108 3.00 0.00 4.108 102 AAACCCTGGCTCCTGCCT 15 18 0 61.11 62.126 3.00 0.00 4.126 103 GACACCCCACTGAGGTCA 39 18 0 61.11 57.806 7.00 4.00 4.194 104 GCAGGTTTAGAAGGGGTGTCTG 66 22 0 54.55 62.245 3.00 1.00 4.245 105 TGCAGGTTTAGAAGGGGTGTCT 65 22 0 50.00 62.245 4.00 0.00 4.245 106 CTGCAGGTTTAGAAGGGGTGTC 64 22 0 54.55 62.245 6.00 1.00 4.245 107 ACCCTGGCTCCTGCCTCT 17 18 0 66.67 62.344 3.00 0.00 4.344 108 GGTTTAGAAGGGGTGTCTGGGT 69 22 0 54.55 62.650 3.00 0.00 4.650 109 AACCCTGGCTCCTGCCTC 16 18 0 66.67 62.728 3.00 0.00 4.728 110 AGGTTTAGAAGGGGTGTCTGGG 68 22 0 54.55 62.752 3.00 0.00 4.752 111 CTGAGGTCAGCACACCCT 48 18 0 61.11 57.145 6.00 2.00 4.855 112 TGTAAACCCTGGCTCCTG 12 18 0 55.56 57.142 3.00 2.00 4.858 113 ACCCTGTAAACCCTGGCT 8 18 0 55.56 57.001 3.00 3.00 4.999