ACCEPTABLE RIGHT PRIMERS 0-based # self self qual- # sequence start ln N GC% Tm any end lity 0 GTGCTTTGCGTTTTGGAAGT 125 20 0 45.00 60.292 3.00 1.00 0.292 1 TGCTTTGCGTTTTGGAAGTT 124 20 0 40.00 60.782 3.00 3.00 0.782 2 GTGCTTTGCGTTTTGGAAG 125 19 0 47.37 59.454 3.00 0.00 1.546 3 TGCTTTGCGTTTTGGAAGT 124 19 0 42.11 59.454 3.00 1.00 1.546 4 TGCTTTGCGTTTTGGAAGTTA 124 21 0 38.10 60.764 3.00 2.00 1.764 5 GCTTTGCGTTTTGGAAGTTA 123 20 0 40.00 58.097 3.00 2.00 1.903 6 GCTTTGCGTTTTGGAAGTTAG 123 21 0 42.86 59.065 3.00 0.00 1.935 7 GAGCGTGCTTTGCGTTTT 129 18 0 50.00 60.142 4.00 0.00 2.142 8 GTGCTTTGCGTTTTGGAAGTT 125 21 0 42.86 61.528 3.00 3.00 2.528 9 GCTTTGCGTTTTGGAAGTTAGA 123 22 0 40.91 60.748 3.00 1.00 2.748 10 GCTTTGCGTTTTGGAAGTT 123 19 0 42.11 58.013 3.00 3.00 2.987 11 AGCGTGCTTTGCGTTTTG 128 18 0 50.00 61.131 4.00 0.00 3.131 12 GCGTTTTGGAAGTTAGACTTTGA 118 23 0 39.13 59.821 6.00 4.00 3.179 13 TGCTTTGCGTTTTGGAAG 124 18 0 44.44 58.546 3.00 0.00 3.454 14 GTGCTTTGCGTTTTGGAAGTTA 125 22 0 40.91 61.484 3.00 2.00 3.484 15 TGCTTTGCGTTTTGGAAGTTAG 124 22 0 40.91 61.593 3.00 0.00 3.593 16 GTGCTTTGCGTTTTGGAA 125 18 0 44.44 58.389 2.00 2.00 3.611 17 TTTGCGTTTTGGAAGTTAGACTT 121 23 0 34.78 59.378 6.00 6.00 3.622 18 TTGCGTTTTGGAAGTTAGACTTT 120 23 0 34.78 59.378 6.00 4.00 3.622 19 TGCGTTTTGGAAGTTAGACTTTG 119 23 0 39.13 60.658 6.00 2.00 3.658 20 CTTTGCGTTTTGGAAGTTAGA 122 21 0 38.10 57.272 3.00 1.00 3.728 21 CTTTGCGTTTTGGAAGTTAGAC 122 22 0 40.91 58.127 3.00 2.00 3.873 22 TTTGCGTTTTGGAAGTTAGACT 121 22 0 36.36 58.127 4.00 4.00 3.873 23 TTGCGTTTTGGAAGTTAGACTT 120 22 0 36.36 58.127 6.00 6.00 3.873 24 TGCGTTTTGGAAGTTAGACTTT 119 22 0 36.36 58.127 6.00 4.00 3.873 25 GCGTTTTGGAAGTTAGACTTTG 118 22 0 40.91 58.127 6.00 2.00 3.873 26 TTTGCGTTTTGGAAGTTAGAC 121 21 0 38.10 57.124 3.00 2.00 3.876 27 CGTGCTTTGCGTTTTGGA 126 18 0 50.00 61.944 2.00 0.00 3.944 28 CTTTGCGTTTTGGAAGTTAGACT 122 23 0 39.13 59.045 4.00 4.00 3.955 29 GAGCGTGCTTTGCGTTTTG 129 19 0 52.63 62.977 4.00 0.00 3.977 30 CTTTGCGTTTTGGAAGTTAGACTT 122 24 0 37.50 60.206 6.00 6.00 4.206 31 CGTTTTGGAAGTTAGACTTTGACA 117 24 0 37.50 59.738 6.00 4.00 4.262 32 GCTTTGCGTTTTGGAAGTTAGAC 123 23 0 43.48 61.438 3.00 2.00 4.438 33 GGAGCGTGCTTTGCGTTT 130 18 0 55.56 62.465 4.00 0.00 4.465 34 TTTGCGTTTTGGAAGTTAGACTTT 121 24 0 33.33 60.517 6.00 4.00 4.517 35 GCGTTTTGGAAGTTAGACTTTGAC 118 24 0 41.67 60.537 6.00 3.00 4.537 36 GATCGGGAGCGTGCTTTG 135 18 0 61.11 62.907 4.00 0.00 4.907 37 GTGCTTTGCGTTTTGGAAGTTAG 125 23 0 43.48 62.256 3.00 0.00 5.256 38 TTGGAAGTTAGACTTTGACAACAGA 113 25 0 36.00 59.394 6.00 3.00 5.606 39 TTTTGGAAGTTAGACTTTGACAACA 115 25 0 32.00 59.292 6.00 4.00 5.708 40 TTGCGTTTTGGAAGTTAGACTTTG 120 24 0 37.50 61.761 6.00 2.00 5.761 41 CGTTTTGGAAGTTAGACTTTGAC 117 23 0 39.13 57.204 6.00 3.00 5.796 42 CGTTTTGGAAGTTAGACTTTGACAA 117 25 0 36.00 60.875 6.00 6.00 5.875 43 TGGAAGTTAGACTTTGACAACAGA 112 24 0 37.50 58.112 6.00 3.00 5.888 44 TTTGGAAGTTAGACTTTGACAACA 114 24 0 33.33 58.041 6.00 3.00 5.959 45 GTTTTGGAAGTTAGACTTTGACAACA 116 26 0 34.62 60.009 8.00 5.00 6.009 46 TTTGGAAGTTAGACTTTGACAACAG 114 25 0 36.00 58.960 6.00 5.00 6.040 47 TGGAAGTTAGACTTTGACAACAGAC 112 25 0 40.00 58.927 6.00 5.00 6.073 48 TTTTGGAAGTTAGACTTTGACAACAG 115 26 0 34.62 60.120 6.00 5.00 6.120 49 TTGGAAGTTAGACTTTGACAACAGAC 113 26 0 38.46 60.127 6.00 5.00 6.127 50 GCTTTGCGTTTTGGAAGTTAGACT 123 24 0 41.67 62.180 4.00 4.00 6.180 51 TGGAAGTTAGACTTTGACAACAGACT 112 26 0 38.46 59.802 7.00 7.00 6.198 52 TGCGTTTTGGAAGTTAGACTTTGA 119 24 0 37.50 62.242 6.00 4.00 6.242 53 CTTTGCGTTTTGGAAGTTAGACTTT 122 25 0 36.00 61.265 6.00 4.00 6.265 54 TTGGAAGTTAGACTTTGACAACAG 113 24 0 37.50 57.684 6.00 5.00 6.316 55 CTTTGACAACAGACTCATACAGTGA 101 25 0 40.00 58.507 5.00 2.00 6.493 56 TTTGACAACAGACTCATACAGTGAG 100 25 0 40.00 58.507 5.00 4.00 6.493 57 TTTGACAACAGACTCATACAGTGA 100 24 0 37.50 57.479 5.00 2.00 6.521 58 CTTTGACAACAGACTCATACAGTGAG 101 26 0 42.31 59.446 5.00 4.00 6.554 59 TTTGGAAGTTAGACTTTGACAACAGA 114 26 0 34.62 60.557 6.00 3.00 6.557 60 GACTTTGACAACAGACTCATACAGTG 103 26 0 42.31 59.320 5.00 3.00 6.680 61 ACTTTGACAACAGACTCATACAGTGA 102 26 0 38.46 59.320 5.00 2.00 6.680 62 TTTGACAACAGACTCATACAGTGAGT 100 26 0 38.46 59.320 6.00 6.00 6.680 63 TTGACAACAGACTCATACAGTGAGTT 99 26 0 38.46 59.320 6.00 4.00 6.680 64 TTGACAACAGACTCATACAGTGAG 99 24 0 41.67 57.083 5.00 4.00 6.917 65 TTGACAACAGACTCATACAGTGAGT 99 25 0 40.00 57.993 6.00 6.00 7.007 66 GGAAGTTAGACTTTGACAACAGACTC 111 26 0 42.31 58.988 7.00 5.00 7.012 67 AGACTTTGACAACAGACTCATACAGTG 104 27 0 40.74 60.192 7.00 3.00 7.192 68 ACTTTGACAACAGACTCATACAGTGAG 102 27 0 40.74 60.192 5.00 4.00 7.192 69 CTTTGACAACAGACTCATACAGTGAGT 101 27 0 40.74 60.192 6.00 6.00 7.192 70 GTTTTGGAAGTTAGACTTTGACAAC 116 25 0 36.00 57.612 8.00 8.00 7.388 71 ACTTTGACAACAGACTCATACAGTG 102 25 0 40.00 57.555 5.00 3.00 7.445 72 TTTGACAACAGACTCATACAGTGAGTT 100 27 0 37.04 60.519 6.00 4.00 7.519 73 CGTTTTGGAAGTTAGACTTTGACAAC 117 26 0 38.46 61.521 8.00 8.00 7.521 74 GGAAGTTAGACTTTGACAACAGACT 111 25 0 40.00 57.318 7.00 7.00 7.682 75 GAAGTTAGACTTTGACAACAGACTCA 110 26 0 38.46 58.271 7.00 7.00 7.729 76 TTTGCGTTTTGGAAGTTAGACTTTG 121 25 0 36.00 62.766 6.00 2.00 7.766 77 GTTTTGGAAGTTAGACTTTGACAACAG 116 27 0 37.04 60.782 8.00 5.00 7.782 78 TGCGTTTTGGAAGTTAGACTTTGAC 119 25 0 40.00 62.860 6.00 3.00 7.860 79 GCGTTTTGGAAGTTAGACTTTGACA 118 25 0 40.00 62.860 6.00 4.00 7.860 80 TTGGAAGTTAGACTTTGACAACAGACT 113 27 0 37.04 60.916 7.00 7.00 7.916 81 GACTTTGACAACAGACTCATACAGTGA 103 27 0 40.74 60.988 5.00 2.00 7.988 82 TTTGGAAGTTAGACTTTGACAACAGAC 114 27 0 37.04 61.219 6.00 5.00 8.219 83 AGACTTTGACAACAGACTCATACAGT 104 26 0 38.46 57.680 7.00 3.00 8.320 84 TGGAAGTTAGACTTTGACAACAGACTC 112 27 0 40.74 61.367 7.00 5.00 8.367 85 GGAAGTTAGACTTTGACAACAGACTCA 111 27 0 40.74 61.367 7.00 7.00 8.367 86 GAAGTTAGACTTTGACAACAGACTCAT 110 27 0 37.04 58.586 7.00 5.00 8.414 87 TTTTGGAAGTTAGACTTTGACAACAGA 115 27 0 33.33 61.617 6.00 3.00 8.617 88 TTAGACTTTGACAACAGACTCATACA 106 26 0 34.62 57.298 7.00 1.00 8.702 89 TTAGACTTTGACAACAGACTCATACAG 106 27 0 37.04 58.234 7.00 1.00 8.766 90 TTGACAACAGACTCATACAGTGAGTTG 99 27 0 40.74 61.830 6.00 6.00 8.830 91 GTTAGACTTTGACAACAGACTCATACA 107 27 0 37.04 58.107 7.00 1.00 8.893 92 TAGACTTTGACAACAGACTCATACAGT 105 27 0 37.04 57.767 7.00 3.00 9.233 93 AAGTTAGACTTTGACAACAGACTCATA 109 27 0 33.33 57.047 7.00 3.00 9.953