ACCEPTABLE LEFT PRIMERS 0-based # self self qual- # sequence start ln N GC% Tm any end lity 0 TATTCCTGTCCCTGTGCCTC 48 20 0 55.00 60.073 2.00 1.00 0.073 1 GCCTCAGAAACCTGGCTACA 63 20 0 55.00 60.397 3.00 1.00 0.397 2 TGCCTCAGAAACCTGGCTAC 62 20 0 55.00 60.397 3.00 3.00 0.397 3 GTGCCTCAGAAACCTGGCTA 61 20 0 55.00 60.397 3.00 2.00 0.397 4 CTGTCCCTGTGCCTCAGAA 53 19 0 57.89 59.963 5.00 1.00 1.037 5 ATTCCTGTCCCTGTGCCTC 49 19 0 57.89 60.064 2.00 1.00 1.064 6 AATATTCCTGTCCCTGTGCC 46 20 0 50.00 58.864 6.00 1.00 1.136 7 AATATTCCTGTCCCTGTGCCT 46 21 0 47.62 59.841 6.00 0.00 1.159 8 CCCTGTGCCTCAGAAACCT 57 19 0 57.89 60.245 5.00 0.00 1.245 9 TGTCCCTGTGCCTCAGAAAC 54 20 0 55.00 61.259 5.00 5.00 1.259 10 ATATTCCTGTCCCTGTGCCTC 47 21 0 52.38 60.342 4.00 1.00 1.342 11 GAAACCTGGCTACAGTTCCC 69 20 0 55.00 58.646 3.00 3.00 1.354 12 AAACCTGGCTACAGTTCCCTC 70 21 0 52.38 59.630 3.00 0.00 1.370 13 GAAACCTGGCTACAGTTCCCT 69 21 0 52.38 59.630 3.00 1.00 1.370 14 AGAAACCTGGCTACAGTTCCC 68 21 0 52.38 59.630 3.00 3.00 1.370 15 TGTCCCTGTGCCTCAGAAA 54 19 0 52.63 60.385 5.00 3.00 1.385 16 CTGTCCCTGTGCCTCAGAAA 53 20 0 55.00 61.390 5.00 3.00 1.390 17 GTGCCTCAGAAACCTGGCT 61 19 0 57.89 60.402 3.00 3.00 1.402 18 TGCCTCAGAAACCTGGCTA 62 19 0 52.63 59.531 3.00 2.00 1.469 19 ATATTCCTGTCCCTGTGCCT 47 20 0 50.00 58.473 4.00 0.00 1.527 20 CTGTGCCTCAGAAACCTGG 59 19 0 57.89 59.399 5.00 3.00 1.601 21 CCTGTGCCTCAGAAACCTG 58 19 0 57.89 59.399 5.00 2.00 1.601 22 AACCTGGCTACAGTTCCCTC 71 20 0 55.00 58.255 3.00 0.00 1.745 23 GTCCCTGTGCCTCAGAAACC 55 20 0 60.00 62.024 5.00 3.00 2.024 24 TAATATTCCTGTCCCTGTGCC 45 21 0 47.62 58.917 6.00 1.00 2.083 25 TAATATTCCTGTCCCTGTGCCT 45 22 0 45.45 59.856 6.00 0.00 2.144 26 TCCCTGTGCCTCAGAAACCT 56 20 0 55.00 62.145 5.00 0.00 2.145 27 CAGAAACCTGGCTACAGTTCC 67 21 0 52.38 58.851 3.00 2.00 2.149 28 CCTCAGAAACCTGGCTACAGTT 64 22 0 50.00 59.810 3.00 2.00 2.190 29 GTGCCTCAGAAACCTGGCTAC 61 21 0 57.14 61.195 3.00 3.00 2.195 30 TTAATATTCCTGTCCCTGTGCC 44 22 0 45.45 60.204 6.00 1.00 2.204 31 AAACCTGGCTACAGTTCCCT 70 20 0 50.00 57.789 3.00 1.00 2.211 32 TCCCTGTGCCTCAGAAACC 56 19 0 57.89 61.213 5.00 1.00 2.213 33 TTCCTGTCCCTGTGCCTC 50 18 0 61.11 59.749 2.00 1.00 2.251 34 GCCTCAGAAACCTGGCTACAG 63 21 0 57.14 61.316 3.00 3.00 2.316 35 CCTGTCCCTGTGCCTCAG 52 18 0 66.67 60.405 5.00 5.00 2.405 36 CCTCAGAAACCTGGCTACAG 64 20 0 55.00 57.547 3.00 3.00 2.453 37 TGCCTCAGAAACCTGGCT 62 18 0 55.56 59.499 3.00 3.00 2.501 38 AGAAACCTGGCTACAGTTCCCT 68 22 0 50.00 60.528 3.00 2.00 2.528 39 CCTCAGAAACCTGGCTACAGT 64 21 0 52.38 58.468 3.00 3.00 2.532 40 GTGCCTCAGAAACCTGGC 61 18 0 61.11 59.345 3.00 2.00 2.655 41 TCAGAAACCTGGCTACAGTTCC 66 22 0 50.00 60.661 3.00 2.00 2.661 42 GTCCCTGTGCCTCAGAAAC 55 19 0 57.89 58.201 5.00 5.00 2.799 43 CCCTGTGCCTCAGAAACC 57 18 0 61.11 59.180 5.00 1.00 2.820 44 TATTCCTGTCCCTGTGCCT 48 19 0 52.63 58.086 2.00 0.00 2.914 45 ATTCCTGTCCCTGTGCCTCA 49 20 0 55.00 62.996 3.00 3.00 2.996 46 CTGTCCCTGTGCCTCAGAAAC 53 21 0 57.14 62.180 5.00 5.00 3.180 47 TGTCCCTGTGCCTCAGAA 54 18 0 55.56 58.812 5.00 1.00 3.188 48 GAAACCTGGCTACAGTTCCCTC 69 22 0 54.55 61.364 3.00 0.00 3.364 49 TGTGCCTCAGAAACCTGGC 60 19 0 57.89 62.397 3.00 2.00 3.397 50 ATTAATATTCCTGTCCCTGTGCC 43 23 0 43.48 60.438 6.00 1.00 3.438 51 TCCTGTCCCTGTGCCTCA 51 18 0 61.11 61.468 3.00 3.00 3.468 52 CCTGTCCCTGTGCCTCAGA 52 19 0 63.16 62.470 5.00 3.00 3.470 53 TCCTGTCCCTGTGCCTCAG 51 19 0 63.16 62.470 5.00 5.00 3.470 54 GCCTCAGAAACCTGGCTAC 63 19 0 57.89 57.465 3.00 3.00 3.535 55 AATATTCCTGTCCCTGTGCCTC 46 22 0 50.00 61.574 6.00 1.00 3.574 56 ATATTCCTGTCCCTGTGCC 47 19 0 52.63 57.370 4.00 1.00 3.630 57 CTGTCCCTGTGCCTCAGA 53 18 0 61.11 58.348 5.00 3.00 3.652 58 TGTGCCTCAGAAACCTGG 60 18 0 55.56 58.249 3.00 3.00 3.751 59 CTCAGAAACCTGGCTACAGTTC 65 22 0 50.00 58.135 3.00 2.00 3.865 60 TTCCTGTCCCTGTGCCTCA 50 19 0 57.89 62.869 3.00 3.00 3.869 61 GTCCCTGTGCCTCAGAAACCT 55 21 0 57.14 62.881 5.00 0.00 3.881 62 TATTCCTGTCCCTGTGCCTCA 48 21 0 52.38 62.891 3.00 3.00 3.891 63 TCAGAAACCTGGCTACAGTTC 66 21 0 47.62 57.065 3.00 2.00 3.935 64 ATTCCTGTCCCTGTGCCT 49 18 0 55.56 57.991 2.00 0.00 4.009 65 TTAATATTCCTGTCCCTGTGCCT 44 23 0 43.48 61.044 6.00 0.00 4.044 66 GCCTCAGAAACCTGGCTACAGT 63 22 0 54.55 62.045 3.00 3.00 4.045 67 CAGAAACCTGGCTACAGTTCCC 67 22 0 54.55 62.245 3.00 3.00 4.245 68 TATTAATATTCCTGTCCCTGTGCC 42 24 0 41.67 60.432 8.00 1.00 4.432 69 CTCAGAAACCTGGCTACAGTTCC 65 23 0 52.17 61.514 3.00 2.00 4.514 70 CCTCAGAAACCTGGCTACAGTTC 64 23 0 52.17 61.514 3.00 2.00 4.514 71 TAATATTCCTGTCCCTGTGCCTC 45 23 0 47.83 61.529 6.00 1.00 4.529 72 TCCCTGTGCCTCAGAAAC 56 18 0 55.56 57.158 5.00 3.00 4.842 73 GTCCCTGTGCCTCAGAAA 55 18 0 55.56 57.158 5.00 3.00 4.842 74 ATTAATATTCCTGTCCCTGTGC 43 22 0 40.91 57.155 6.00 2.00 4.845 75 AGAAACCTGGCTACAGTTCCCTC 68 23 0 52.17 62.164 3.00 0.00 5.164 76 ATTAATATTCCTGTCCCTGTGCCT 43 24 0 41.67 61.230 6.00 0.00 5.230 77 ATATTAATATTCCTGTCCCTGTGCC 41 25 0 40.00 60.638 10.00 1.00 5.638 78 TATTAATATTCCTGTCCCTGTGC 42 23 0 39.13 57.263 8.00 2.00 5.737 79 AAATATTAATATTCCTGTCCCTGTGC 39 26 0 34.62 59.917 12.00 2.00 6.083 80 AATATTAATATTCCTGTCCCTGTGC 40 25 0 36.00 58.823 12.00 2.00 6.177 81 TATTAATATTCCTGTCCCTGTGCCT 42 25 0 40.00 61.200 8.00 0.00 6.200 82 ATATTAATATTCCTGTCCCTGTGC 41 24 0 37.50 57.627 10.00 2.00 6.373 83 TTAATATTCCTGTCCCTGTGCCTC 44 24 0 45.83 62.607 6.00 1.00 6.607 84 CAAATATTAATATTCCTGTCCCTGTG 38 26 0 34.62 59.176 12.00 1.00 6.824 85 TCAAATATTAATATTCCTGTCCCTG 37 25 0 32.00 57.690 12.00 1.00 7.310 86 ATATTAATATTCCTGTCCCTGTGCCT 41 26 0 38.46 61.365 10.00 0.00 7.365 87 CTCAAATATTAATATTCCTGTCCCTG 36 26 0 34.62 58.540 12.00 1.00 7.460 88 TCAAATATTAATATTCCTGTCCCTGT 37 26 0 30.77 58.425 12.00 0.00 7.575 89 TCAAATATTAATATTCCTGTCCCTGTG 37 27 0 33.33 60.641 12.00 1.00 7.641 90 AATATTAATATTCCTGTCCCTGTGCC 40 26 0 38.46 61.650 12.00 1.00 7.650 91 ATTAATATTCCTGTCCCTGTGCCTC 43 25 0 44.00 62.718 6.00 1.00 7.718 92 CTCAAATATTAATATTCCTGTCCCTGT 36 27 0 33.33 59.222 12.00 0.00 7.778 93 TATTAATATTCCTGTCCCTGTGCCTC 42 26 0 42.31 62.643 8.00 1.00 8.643 94 CAAATATTAATATTCCTGTCCCTGTGC 38 27 0 37.04 62.049 12.00 2.00 9.049 95 AATATTAATATTCCTGTCCCTGTGCCT 40 27 0 37.04 62.311 12.00 0.00 9.311 96 AAATATTAATATTCCTGTCCCTGTGCC 39 27 0 37.04 62.579 12.00 1.00 9.579 97 ATATTAATATTCCTGTCCCTGTGCCTC 41 27 0 40.74 62.745 10.00 1.00 9.745