ACCEPTABLE RIGHT PRIMERS 0-based # self self qual- # sequence start ln N GC% Tm any end lity 0 TCCCTCATCCAGTGCTCTTT 184 20 0 50.00 59.803 4.00 0.00 0.197 1 CCCTCATCCAGTGCTCTTTC 183 20 0 55.00 59.803 4.00 0.00 0.197 2 CAGTGCTCTTTCTGCCAGTG 175 20 0 55.00 59.770 5.00 3.00 0.230 3 CCAGTGCTCTTTCTGCCAGT 176 20 0 55.00 60.589 5.00 3.00 0.589 4 TGCTCTTTCTGCCAGTGTCA 172 20 0 50.00 60.745 5.00 5.00 0.745 5 GATCCCTCATCCAGTGCTCT 186 20 0 55.00 59.210 4.00 4.00 0.790 6 TCATCCAGTGCTCTTTCTGC 180 20 0 50.00 59.116 4.00 2.00 0.884 7 CATCCAGTGCTCTTTCTGCC 179 20 0 55.00 60.945 4.00 1.00 0.945 8 ATCCAGTGCTCTTTCTGCCA 178 20 0 50.00 60.945 4.00 3.00 0.945 9 CCTCATCCAGTGCTCTTTCTG 182 21 0 52.38 59.997 4.00 2.00 1.003 10 ATCCCTCATCCAGTGCTCTTT 185 21 0 47.62 60.088 4.00 0.00 1.088 11 CTCATCCAGTGCTCTTTCTGC 181 21 0 52.38 60.149 4.00 2.00 1.149 12 CCAGTGCTCTTTCTGCCAG 176 19 0 57.89 59.710 5.00 5.00 1.290 13 ATCCCTCATCCAGTGCTCTT 185 20 0 50.00 58.693 4.00 2.00 1.307 14 AGTGCTCTTTCTGCCAGTGTC 174 21 0 52.38 59.661 5.00 3.00 1.339 15 GTGCTCTTTCTGCCAGTGTC 173 20 0 55.00 58.596 5.00 3.00 1.404 16 GCTCTTTCTGCCAGTGTCAC 171 20 0 55.00 58.596 5.00 5.00 1.404 17 GATCCCTCATCCAGTGCTCTT 186 21 0 52.38 60.611 4.00 2.00 1.611 18 CAGTGCTCTTTCTGCCAGTGT 175 21 0 52.38 60.638 5.00 1.00 1.638 19 TCCAGTGCTCTTTCTGCCAG 177 20 0 55.00 61.674 5.00 5.00 1.674 20 TCCAGTGCTCTTTCTGCCA 177 19 0 52.63 60.694 4.00 3.00 1.694 21 CCCTCATCCAGTGCTCTTTCT 183 21 0 52.38 60.771 4.00 0.00 1.771 22 GGCCCTCTGGTTAACCGT 148 18 0 61.11 59.927 8.00 4.00 2.073 23 AGTGCTCTTTCTGCCAGTGT 174 20 0 50.00 57.670 5.00 1.00 2.330 24 GTGCTCTTTCTGCCAGTGTCA 173 21 0 52.38 61.588 5.00 5.00 2.588 25 TGCTCTTTCTGCCAGTGTCAC 172 21 0 52.38 61.588 5.00 5.00 2.588 26 TCCCTCATCCAGTGCTCTTTC 184 21 0 52.38 61.686 4.00 0.00 2.686 27 TCCCTCATCCAGTGCTCTT 184 19 0 52.63 58.306 4.00 2.00 2.694 28 ATCCAGTGCTCTTTCTGCCAG 178 21 0 52.38 61.860 5.00 5.00 2.860 29 GCCCTCTGGTTAACCGTG 147 18 0 61.11 59.086 8.00 6.00 2.914 30 GATCCCTCATCCAGTGCTC 186 19 0 57.89 58.080 4.00 2.00 2.920 31 CCTCATCCAGTGCTCTTTCT 182 20 0 50.00 57.032 4.00 0.00 2.968 32 ATCCAGTGCTCTTTCTGCC 178 19 0 52.63 57.954 4.00 1.00 3.046 33 CCCTCATCCAGTGCTCTTT 183 19 0 52.63 57.795 4.00 0.00 3.205 34 TGCTCTTTCTGCCAGTGTC 172 19 0 52.63 57.590 5.00 3.00 3.410 35 GCTCTTTCTGCCAGTGTCA 171 19 0 52.63 57.590 5.00 5.00 3.410 36 CCAGTGCTCTTTCTGCCA 176 18 0 55.56 58.588 4.00 3.00 3.412 37 TCCAGTGCTCTTTCTGCCAGT 177 21 0 52.38 62.447 5.00 3.00 3.447 38 TTGGCCCTCTGGTTAACC 150 18 0 55.56 58.487 8.00 8.00 3.513 39 TGGCCCTCTGGTTAACCGT 149 19 0 57.89 62.761 8.00 4.00 3.761 40 GGCCCTCTGGTTAACCGTG 148 19 0 63.16 62.761 8.00 6.00 3.761 41 TCATCCAGTGCTCTTTCTGCC 180 21 0 52.38 62.794 4.00 1.00 3.794 42 GATCCCTCATCCAGTGCTCTTT 186 22 0 50.00 61.864 4.00 0.00 3.864 43 ATCCCTCATCCAGTGCTCTTTC 185 22 0 50.00 61.864 4.00 0.00 3.864 44 ATCCCTCATCCAGTGCTCT 185 19 0 52.63 57.128 4.00 4.00 3.872 45 CATCCAGTGCTCTTTCTGC 179 19 0 52.63 57.014 4.00 2.00 3.986 46 TGGCCCTCTGGTTAACCG 149 18 0 61.11 62.001 8.00 6.00 4.001 47 GTGCTCTTTCTGCCAGTGTCAC 173 22 0 54.55 62.364 5.00 5.00 4.364 48 CAGTGCTCTTTCTGCCAGTGTC 175 22 0 54.55 62.479 5.00 3.00 4.479 49 AGTGCTCTTTCTGCCAGTGTCA 174 22 0 50.00 62.479 5.00 5.00 4.479 50 TCCAGTGCTCTTTCTGCC 177 18 0 55.56 57.507 4.00 1.00 4.493 51 TCCCTCATCCAGTGCTCTTTCT 184 22 0 50.00 62.539 4.00 0.00 4.539 52 ATCCAGTGCTCTTTCTGCCAGT 178 22 0 50.00 62.585 5.00 3.00 4.585 53 ATCCCTCATCCAGTGCTCTTTCT 185 23 0 47.83 62.667 4.00 0.00 5.667