/* * Copyright (c) 2005, 2007 Genome Research Ltd. * Author(s): James Bonfield * * Redistribution and use in source and binary forms, with or without * modification, are permitted provided that the following conditions are met: * * 1. Redistributions of source code must retain the above copyright notice, * this list of conditions and the following disclaimer. * * 2. Redistributions in binary form must reproduce the above * copyright notice, this list of conditions and the following * disclaimer in the documentation and/or other materials provided * with the distribution. * * 3. Neither the names Genome Research Ltd and Wellcome Trust Sanger * Institute nor the names of its contributors may be used to endorse * or promote products derived from this software without specific * prior written permission. * * THIS SOFTWARE IS PROVIDED BY GENOME RESEARCH LTD AND CONTRIBUTORS "AS * IS" AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED * TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A * PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL GENOME RESEARCH * LTD OR CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, * SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT * LIMITED TO, PROCUREMENT OF SUBSTITUTE GOODS OR SERVICES; LOSS OF USE, * DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON ANY * THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT * (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE * OF THIS SOFTWARE, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE. */ #ifndef _SFF_H_ #define _SFF_H_ #include "io_lib/Read.h" #include "io_lib/os.h" #include "io_lib/mFILE.h" #ifdef __cplusplus extern "C" { #endif /* * This mirrors the order that the SFF header has on disc. We have one of * these only. * * The flow and key are variable length fields as defined by flow_len * and key_len. * The on-disc structure is then also padded out with zeros to the 8-byte * word boundary. */ typedef struct { uint32_t magic; char version[4]; uint64_t index_offset; uint32_t index_len; uint32_t nreads; uint16_t header_len; uint16_t key_len; uint16_t flow_len; uint8_t flowgram_format; char *flow; char *key; } sff_common_header; #define SFF_MAGIC 0x2e736666 /* ".sff" */ #define SFF_VERSION "\0\0\0\1" /* * We have one read_header per "reading" in the SFF archive. * It too is padded to an 8-byte boundary. */ typedef struct { uint16_t header_len; uint16_t name_len; uint32_t nbases; uint16_t clip_qual_left; uint16_t clip_qual_right; uint16_t clip_adapter_left; uint16_t clip_adapter_right; char *name; } sff_read_header; /* * We have one read_data section per reading, following the read_header. * It is padded to an 8-byte boundary. */ typedef struct { uint16_t *flowgram; /* x 100.0 */ uint8_t *flow_index; /* relative to last */ char *bases; uint8_t *quality; } sff_read_data; /* * Low level functions to decode SFF internals */ sff_common_header *decode_sff_common_header(unsigned char *buf); sff_common_header *read_sff_common_header(mFILE *mf); void free_sff_common_header(sff_common_header *h); sff_read_header *decode_sff_read_header(unsigned char *buf); void free_sff_read_header(sff_read_header *h); sff_read_header *read_sff_read_header(mFILE *mf); void free_sff_read_data(sff_read_data *d); sff_read_data *read_sff_read_data(mFILE *mf, int nflows, int nbases); /* * Loads the first SFF sequence from an SFF container and returns as a Read. */ Read *mfread_sff(mFILE *mf); #ifdef __cplusplus } #endif #endif /* _SFF_H_ */