/* Tests for fqz codec */ /* * Copyright (c) 2019,2020 Genome Research Ltd. * Author(s): James Bonfield * * Redistribution and use in source and binary forms, with or without * modification, are permitted provided that the following conditions are met: * * 1. Redistributions of source code must retain the above copyright notice, * this list of conditions and the following disclaimer. * * 2. Redistributions in binary form must reproduce the above * copyright notice, this list of conditions and the following * disclaimer in the documentation and/or other materials provided * with the distribution. * * 3. Neither the names Genome Research Ltd and Wellcome Trust Sanger * Institute nor the names of its contributors may be used to endorse * or promote products derived from this software without specific * prior written permission. * * THIS SOFTWARE IS PROVIDED BY GENOME RESEARCH LTD AND CONTRIBUTORS "AS * IS" AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED * TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A * PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL GENOME RESEARCH * LTD OR CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, * SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT * LIMITED TO, PROCUREMENT OF SUBSTITUTE GOODS OR SERVICES; LOSS OF USE, * DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON ANY * THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT * (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE * OF THIS SOFTWARE, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE. */ #include #include #include #include #include #include #include #include #include #include "htscodecs/fqzcomp_qual.h" #include "htscodecs/varint.h" #ifndef MAX_REC #define MAX_REC 1000000 #endif #ifndef MAX_SEQ # define MAX_SEQ 100000 #endif #ifndef MIN # define MIN(a,b) ((a)<(b)?(a):(b)) # define MAX(a,b) ((a)>(b)?(a):(b)) #endif static fqz_slice fixed_slice = {0}; fqz_slice *fake_slice(size_t buf_len, int *len, int *r2, int *sel, int nlen) { fixed_slice.num_records = (nlen == 1) ? (buf_len+len[0]-1) / len[0] : nlen; assert(fixed_slice.num_records <= MAX_REC); int i; if (!fixed_slice.len) fixed_slice.len = malloc(MAX_REC * sizeof(*fixed_slice.len)); if (!fixed_slice.flags) fixed_slice.flags = malloc(MAX_REC * sizeof(*fixed_slice.flags)); for (i = 0; i < fixed_slice.num_records; i++) { int idx = i < nlen ? i : nlen-1; fixed_slice.len[i] = len[idx]; fixed_slice.flags[i] = r2 ? r2[idx]*FQZ_FREAD2 : 0; fixed_slice.flags[i] |= sel ? (sel[idx]<<16) : 0; } return &fixed_slice; } static uint64_t manual_strats[10] = {0}; static int manual_nstrat = 0; /* * Manually specified strategies held in global manual_strats[]. */ static inline int fqz_manual_parameters(fqz_gparams *gp, fqz_slice *s, unsigned char *in, size_t in_size) { int i, p; int dsqr[] = { 0, 1, 1, 1, 2, 2, 2, 2, 2, 3, 3, 3, 3, 3, 3, 3, 4, 4, 4, 4, 4, 4, 4, 4, 4, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 5, 6, 6, 6, 6, 6, 6, 6, 6, 6, 6, 6, 6, 6, 7, 7, 7, 7, 7, 7, 7, 7, 7, 7, 7, 7, 7, 7, 7 }; gp->vers = FQZ_VERS; gp->nparam = manual_nstrat; gp->gflags = GFLAG_MULTI_PARAM | GFLAG_HAVE_STAB; for (i = 0; i < 256; i++) gp->stab[i] = 0; // Fill these out later gp->max_sel = 0; gp->max_sym = 0; gp->p = malloc(gp->nparam * sizeof(*gp->p)); for (p = 0; p < gp->nparam; p++) { fqz_param *pm = &gp->p[p]; uint64_t st = manual_strats[p]; pm->do_qa = st & 15; st >>= 4; pm->do_r2 = st & 15; st >>= 4; pm->dloc = st & 15; st >>= 4; pm->ploc = st & 15; st >>= 4; pm->sloc = st & 15; st >>= 4; pm->qloc = st & 15; st >>= 4; pm->dshift = st & 15; st >>= 4; pm->dbits = st & 15; st >>= 4; pm->pshift = st & 15; st >>= 4; pm->pbits = st & 15; st >>= 4; pm->qshift = st & 15; st >>= 4; pm->qbits = st & 15; st >>= 4; // Gather some stats, as per qual_stats func. // r in rec count. // i = index to in[] // j = index within this rec uint32_t qhist[256] = {0}; // qual stats for seqs using this parameter only fqz_qual_stats(s, in, in_size, pm, qhist, p); int max_sel = pm->max_sel; // Update max_sel running total. Eg with 4 sub-params: // // sel param no. => new // 0 0 0 // 0/1 1 1,2 // 0/1 2 3,4 // 0 3 5 for (i = gp->max_sel; i < gp->max_sel + max_sel+1; i++) gp->stab[i] = p; gp->max_sel += max_sel+1; pm->fixed_len = pm->fixed_len > 0; pm->use_qtab = 0; // unused by current encoder pm->store_qmap = pm->nsym <= 8; // Adjust parameters based on quality stats. // FIXME: dup from fqz_pick_parameters. for (i = 0; i < sizeof(dsqr)/sizeof(*dsqr); i++) if (dsqr[i] > (1<dbits)-1) dsqr[i] = (1<dbits)-1; if (pm->store_qmap) { int j; for (i = j = 0; i < 256; i++) if (qhist[i]) pm->qmap[i] = j++; else pm->qmap[i] = INT_MAX; pm->max_sym = pm->nsym; } else { pm->nsym = 255; for (i = 0; i < 256; i++) pm->qmap[i] = i; } if (gp->max_sym < pm->max_sym) gp->max_sym = pm->max_sym; // Produce ptab from pshift. if (pm->qbits) { for (i = 0; i < 256; i++) { pm->qtab[i] = i; // 1:1 // Alternative mappings: //qtab[i] = i > 30 ? MIN(max_sym,i)-15 : i/2; // eg for 9827 BAM } } pm->qmask = (1<qbits)-1; if (pm->pbits) { for (i = 0; i < 1024; i++) pm->ptab[i] = MIN((1<pbits)-1, i>>pm->pshift); // Alternatively via analysis of quality distributions we // may select a bunch of positions that are special and // have a non-uniform ptab[]. // Manual experimentation on a NovaSeq run saved 2.8% here. } if (pm->dbits) { for (i = 0; i < 256; i++) pm->dtab[i] = dsqr[MIN(sizeof(dsqr)/sizeof(*dsqr)-1, i>>pm->dshift)]; } pm->use_ptab = (pm->pbits > 0); pm->use_dtab = (pm->dbits > 0); pm->pflags = (pm->use_qtab ?PFLAG_HAVE_QTAB :0)| (pm->use_dtab ?PFLAG_HAVE_DTAB :0)| (pm->use_ptab ?PFLAG_HAVE_PTAB :0)| (pm->do_sel ?PFLAG_DO_SEL :0)| (pm->fixed_len ?PFLAG_DO_LEN :0)| (pm->do_dedup ?PFLAG_DO_DEDUP :0)| (pm->store_qmap ?PFLAG_HAVE_QMAP :0); } for (i = gp->max_sel; i < 256; i++) gp->stab[i] = gp->stab[gp->max_sel-1]; return 0; } #define BS 1024*1024 static unsigned char *load(char *fn, size_t *lenp) { unsigned char *data = NULL; uint64_t dsize = 0; uint64_t dcurr = 0; signed int len; //build_rcp_freq(); #ifndef _O_BINARY #define _O_BINARY 0 #endif int fd = open(fn, O_RDONLY | _O_BINARY); if (!fd) { perror(fn); return NULL; } do { if (dsize - dcurr < BS) { dsize = dsize ? dsize * 2 : BS; data = realloc(data, dsize); } len = read(fd, data + dcurr, BS); if (len > 0) dcurr += len; } while (len > 0); if (len == -1) { perror("read"); } close(fd); *lenp = dcurr; return data; } #define BLK_SIZE 300*1000000 //#define BLK_SIZE 100*100000 int count_lines(unsigned char *in, size_t len) { size_t i; int lines = 0; for (i = 0; i < len; i++) if (in[i] == '\n') lines++; return lines; } // QUAL [is_read2 [selector]] void parse_lines(unsigned char *in, size_t len, int *rec_len, int *rec_r2, int *rec_sel, size_t *new_len) { size_t i, j, start; int rec = 0; for (start = i = j = 0; i < len; i++) { if (in[i] == '\n' || in[i] == ' ' || in[i] == '\t') { rec_len[rec] = i-start; // Read2 marker while (i < len && in[i] != '\n' && isspace(in[i])) i++; if (in[i] != '\n') rec_r2[rec] = atoi((char *)&in[i]); else rec_r2[rec] = 0; while (i < len && !isspace(in[i])) i++; // selector while (i < len && in[i] != '\n' && isspace(in[i])) i++; if (in[i] != '\n') rec_sel[rec] = atoi((char *)&in[i]); else rec_sel[rec] = 0; while (i < len && in[i] != '\n') i++; start = i+1; rec++; } else { in[j++] = in[i]-33; // ASCII phred to qual } } *new_len = j; } int main(int argc, char **argv) { unsigned char *in, *out; size_t in_len, out_len; int decomp = 0, vers = 4; // CRAM version 4.0 (4) or 3.1 (3) int strat = 0, raw = 0; fqz_gparams *gp = NULL, gp_local; int blk_size = BLK_SIZE; // MAX #ifdef _WIN32 _setmode(_fileno(stdin), _O_BINARY); _setmode(_fileno(stdout), _O_BINARY); #endif extern char *optarg; extern int optind; int opt; while ((opt = getopt(argc, argv, "ds:s:b:rx:")) != -1) { switch (opt) { case 'd': decomp = 1; break; case 'b': blk_size = atoi(optarg); if (blk_size > BLK_SIZE) blk_size = BLK_SIZE; break; case 's': strat = atoi(optarg); break; case 'x': { // Hex digits are: // qbits qshift // pbits pshift // dbits dshift // qloc sloc // ploc dloc // do_r2 do_qavg // // Examples: -x 0x5570000d6e14 q40+dir = 3473340 // -x 0x8252120e8d04 q4 = 724989 uint64_t x = strtol(optarg, NULL, 0); manual_strats[manual_nstrat++] = x; gp = &gp_local; break; } case 'r': raw = 1; break; } } in = load(optind < argc ? argv[optind] : "/dev/stdin", &in_len); if (!in) exit(1); if (raw) blk_size = in_len; // Block based, for arbitrary sizes of input if (decomp) { unsigned char *in2 = in; while (in_len > 0) { // Read sizes as 32-bit size_t in2_len, out_len; if (raw) { uint32_t u32; var_get_u32(in2, in2+in_len, &u32); out_len = u32; in2_len = in_len; } else { out_len = *(uint32_t *)in2; in2 += 4; in2_len = *(uint32_t *)in2; in2 += 4; } fprintf(stderr, "out_len %ld, in_len %ld\n", (long)out_len, (long)in2_len); int *lengths = malloc(MAX_REC * sizeof(int)); out = (unsigned char *)fqz_decompress((char *)in2, in_len-(raw?0:8), &out_len, lengths, MAX_REC); if (!out) { fprintf(stderr, "Failed to decompress\n"); return 1; } // Convert from binary back to ASCII with newlines int i = 0, j = 0; while (j < out_len) { int k; char seq[MAX_SEQ]; for (k = 0; k < lengths[i]; k++) seq[k] = out[j+k]+33; seq[k] = 0; puts(seq); j += lengths[i++]; } free(out); in2 += in2_len; in_len -= in2_len+(raw?0:8); free(lengths); break; // One cycle only until we fix blocking to be \n based } } else { // Convert from ASCII newline separated file to binary block. // We return an array of line lengths and optionally param selectors. int nlines = count_lines(in, in_len); fprintf(stderr, "nlines=%d\n", nlines); int *rec_len = calloc(nlines, sizeof(*rec_len)); int *rec_r2 = calloc(nlines, sizeof(*rec_r2)); int *rec_sel = calloc(nlines, sizeof(*rec_sel)); parse_lines(in, in_len, rec_len, rec_r2, rec_sel, &in_len); unsigned char *in2 = in; long t_out = 0; out = NULL; while (in_len > 0) { // FIXME: blk_size no longer working in test. One cycle only! size_t in2_len = in_len <= blk_size ? in_len : blk_size; fqz_slice *s = fake_slice(in2_len, rec_len, rec_r2, rec_sel, nlines); if (gp == &gp_local) if (fqz_manual_parameters(gp, s, in2, in2_len) < 0) return 1; out = (unsigned char *)fqz_compress(vers, s, (char *)in2, in2_len, &out_len, strat, gp); // Write out 32-bit sizes. if (!raw) { uint32_t u32; u32 = in2_len; if (write(1, &u32, 4) != 4) return 1; u32 = out_len; if (write(1, &u32, 4) != 4) return 1; } if (write(1, out, out_len) < 0) return 1; in_len -= in2_len; in2 += in2_len; t_out += out_len + (raw?0:8); break; // One cycle only until we fix blocking to be \n based } free(out); free(rec_len); free(rec_r2); free(rec_sel); fprintf(stderr, "Total output = %ld\n", t_out); } free(in); return 0; }