/* bam_index.c -- index and idxstats subcommands. Copyright (C) 2008-2011, 2013-2016, 2018, 2019 Genome Research Ltd. Portions copyright (C) 2010 Broad Institute. Portions copyright (C) 2013 Peter Cock, The James Hutton Institute. Author: Heng Li Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions: The above copyright notices and this permission notice shall be included in all copies or substantial portions of the Software. THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE. */ #include #include #include #include #include #include #include #include #include #include #include "samtools.h" #include "sam_opts.h" #define BAM_LIDX_SHIFT 14 static void index_usage(FILE *fp) { fprintf(fp, "Usage: samtools index -M [-bc] [-m INT] ...\n" " or: samtools index [-bc] [-m INT] [out.index]\n" "Options:\n" " -b Generate BAI-format index for BAM files [default]\n" " -c Generate CSI-format index for BAM files\n" " -m INT Set minimum interval size for CSI indices to 2^INT [%d]\n" " -M Interpret all filename arguments as files to be indexed\n" " -o FILE Write index to FILE [alternative to as an argument]\n" " -@ INT Sets the number of threads [none]\n", BAM_LIDX_SHIFT); } // Returns 1 if the file does not exist or can be positively // identified as an index file. static int nonexistent_or_index(const char *fn) { int ret1, ret2; htsFormat fmt; hFILE *fp = hopen(fn, "r"); if (fp == NULL) return 1; ret1 = hts_detect_format2(fp, fn, &fmt); ret2 = hclose(fp); if (ret1 < 0 || ret2 < 0) return 0; return fmt.category == index_file; } int bam_index(int argc, char *argv[]) { int csi = 0; int min_shift = BAM_LIDX_SHIFT; int multiple = 0; int n_threads = 0; int n_files, c, i, ret; const char *fn_idx = NULL; while ((c = getopt(argc, argv, "bcm:Mo:@:")) >= 0) switch (c) { case 'b': csi = 0; break; case 'c': csi = 1; break; case 'm': csi = 1; min_shift = atoi(optarg); break; case 'M': multiple = 1; break; case 'o': fn_idx = optarg; break; case '@': n_threads = atoi(optarg); break; default: index_usage(stderr); return 1; } n_files = argc - optind; if (n_files == 0) { index_usage(stdout); return 0; } // Handle legacy synopsis if (n_files == 2 && !fn_idx && nonexistent_or_index(argv[optind+1])) { n_files = 1; fn_idx = argv[optind+1]; } if (n_files > 1 && !multiple) { print_error("index", "use -M to enable indexing more than one alignment file"); return EXIT_FAILURE; } if (fn_idx && n_files > 1) { // TODO In future we may allow %* placeholders or similar print_error("index", "can't use -o with multiple input alignment files"); return EXIT_FAILURE; } for (i = optind; i < optind + n_files; i++) { ret = sam_index_build3(argv[i], fn_idx, csi? min_shift : 0, n_threads); if (ret < 0) { if (ret == -2) print_error_errno("index", "failed to open \"%s\"", argv[i]); else if (ret == -3) print_error("index", "\"%s\" is in a format that cannot be usefully indexed", argv[i]); else if (ret == -4 && fn_idx) print_error("index", "failed to create or write index \"%s\"", fn_idx); else if (ret == -4) print_error("index", "failed to create or write index"); else print_error_errno("index", "failed to create index for \"%s\"", argv[i]); return EXIT_FAILURE; } } return EXIT_SUCCESS; } /* * Cram indices do not contain mapped/unmapped record counts, so we have to * decode each record and count. However we can speed this up as much as * possible by using the required fields parameter. * * This prints the stats to stdout in the same manner than the BAM function * does. * * Returns 0 on success, * -1 on failure. */ int slow_idxstats(samFile *fp, sam_hdr_t *header) { int ret, last_tid = -2; bam1_t *b = bam_init1(); if (hts_set_opt(fp, CRAM_OPT_REQUIRED_FIELDS, SAM_RNAME | SAM_FLAG)) return -1; uint64_t (*count0)[2] = calloc(sam_hdr_nref(header)+1, sizeof(*count0)); uint64_t (*counts)[2] = count0+1; if (!count0) return -1; while ((ret = sam_read1(fp, header, b)) >= 0) { if (b->core.tid >= sam_hdr_nref(header) || b->core.tid < -1) { free(count0); return -1; } if (b->core.tid != last_tid) { if (last_tid >= -1) { if (counts[b->core.tid][0] + counts[b->core.tid][1]) { print_error("idxstats", "file is not position sorted"); free(count0); return -1; } } last_tid = b->core.tid; } counts[b->core.tid][(b->core.flag & BAM_FUNMAP) ? 1 : 0]++; } if (ret == -1) { int i; for (i = 0; i < sam_hdr_nref(header); i++) { printf("%s\t%"PRId64"\t%"PRIu64"\t%"PRIu64"\n", sam_hdr_tid2name(header, i), (int64_t) sam_hdr_tid2len(header, i), counts[i][0], counts[i][1]); } printf("*\t0\t%"PRIu64"\t%"PRIu64"\n", counts[-1][0], counts[-1][1]); } free(count0); bam_destroy1(b); return (ret == -1) ? 0 : -1; } static void usage_exit(FILE *fp, int exit_status) { fprintf(fp, "Usage: samtools idxstats [options] \n"); sam_global_opt_help(fp, "-.---@-."); exit(exit_status); } int bam_idxstats(int argc, char *argv[]) { hts_idx_t* idx; sam_hdr_t* header; samFile* fp; int c; sam_global_args ga = SAM_GLOBAL_ARGS_INIT; static const struct option lopts[] = { SAM_OPT_GLOBAL_OPTIONS('-', 0, '-', '-', '-', '@'), {NULL, 0, NULL, 0} }; while ((c = getopt_long(argc, argv, "@:", lopts, NULL)) >= 0) { switch (c) { default: if (parse_sam_global_opt(c, optarg, lopts, &ga) == 0) break; /* else fall-through */ case '?': usage_exit(stderr, EXIT_FAILURE); } } if (argc != optind+1) { if (argc == optind) usage_exit(stdout, EXIT_SUCCESS); else usage_exit(stderr, EXIT_FAILURE); } fp = sam_open_format(argv[optind], "r", &ga.in); if (fp == NULL) { print_error_errno("idxstats", "failed to open \"%s\"", argv[optind]); return 1; } header = sam_hdr_read(fp); if (header == NULL) { print_error("idxstats", "failed to read header for \"%s\"", argv[optind]); return 1; } if (hts_get_format(fp)->format != bam) { slow_method: if (ga.nthreads) hts_set_threads(fp, ga.nthreads); if (slow_idxstats(fp, header) < 0) { print_error("idxstats", "failed to process \"%s\"", argv[optind]); return 1; } } else { idx = sam_index_load(fp, argv[optind]); if (idx == NULL) { print_error("idxstats", "fail to load index for \"%s\", " "reverting to slow method", argv[optind]); goto slow_method; } int i; for (i = 0; i < sam_hdr_nref(header); ++i) { // Print out contig name and length printf("%s\t%"PRId64, sam_hdr_tid2name(header, i), (int64_t) sam_hdr_tid2len(header, i)); // Now fetch info about it from the meta bin uint64_t u, v; hts_idx_get_stat(idx, i, &u, &v); printf("\t%" PRIu64 "\t%" PRIu64 "\n", u, v); } // Dump information about unmapped reads printf("*\t0\t0\t%" PRIu64 "\n", hts_idx_get_n_no_coor(idx)); hts_idx_destroy(idx); } sam_hdr_destroy(header); sam_close(fp); return 0; }