/* faidx.c -- faidx subcommand. Copyright (C) 2008, 2009, 2013, 2016, 2018-2020, 2022 Genome Research Ltd. Portions copyright (C) 2011 Broad Institute. Author: Heng Li Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions: The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software. THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE. History: * 2016-01-12: Pierre Lindenbaum @yokofakun : added options -o -n */ #include #include #include #include #include #include #include #include #include #include #include #include #include #include #include "samtools.h" // Negative indicates the same as input data #define DEFAULT_FASTA_LINE_LEN -60 #ifndef ABS # define ABS(x) ((x)>=0?(x):-(x)) #endif static unsigned char comp_base[256] = { 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, '!', '"', '#', '$', '%', '&', '\'','(', ')', '*', '+', ',', '-', '.', '/', '0', '1', '2', '3', '4', '5', '6', '7', '8', '9', ':', ';', '<', '=', '>', '?', '@', 'T', 'V', 'G', 'H', 'E', 'F', 'C', 'D', 'I', 'J', 'M', 'L', 'K', 'N', 'O', 'P', 'Q', 'Y', 'S', 'A', 'A', 'B', 'W', 'X', 'R', 'Z', '[', '\\',']', '^', '_', '`', 't', 'v', 'g', 'h', 'e', 'f', 'c', 'd', 'i', 'j', 'm', 'l', 'k', 'n', 'o', 'p', 'q', 'y', 's', 'a', 'a', 'b', 'w', 'x', 'r', 'z', '{', '|', '}', '~', 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 219, 220, 221, 222, 223, 224, 225, 226, 227, 228, 229, 230, 231, 232, 233, 234, 235, 236, 237, 238, 239, 240, 241, 242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252, 253, 254, 255, }; static void reverse_complement(char *str, const hts_pos_t len) { char c; hts_pos_t i = 0, j = len - 1; while (i <= j) { c = str[i]; str[i] = comp_base[(unsigned char)str[j]]; str[j] = comp_base[(unsigned char)c]; i++; j--; } } static void reverse(char *str, const hts_pos_t len) { char c; hts_pos_t i = 0, j = len - 1; while (i < j) { c = str[i]; str[i] = str[j]; str[j] = c; i++; j--; } } static int write_line(faidx_t *faid, FILE *file, const char *line, const char *name, const int ignore, const hts_pos_t length, const hts_pos_t seq_len) { int id; hts_pos_t beg, end; if (seq_len < 0) { fprintf(stderr, "[faidx] Failed to fetch sequence in %s\n", name); if (ignore && seq_len == -2) { return EXIT_SUCCESS; } else { return EXIT_FAILURE; } } else if (seq_len == 0) { fprintf(stderr, "[faidx] Zero length sequence: %s\n", name); } else if (fai_parse_region(faid, name, &id, &beg, &end, 0) && (end < HTS_POS_MAX) && (seq_len != end - beg)) { fprintf(stderr, "[faidx] Truncated sequence: %s\n", name); } hts_pos_t i, seq_sz = seq_len; for (i = 0; i < seq_sz; i += length) { hts_pos_t len = i + length < seq_sz ? length : seq_sz - i; if (fwrite(line + i, 1, len, file) < len || fputc('\n', file) == EOF) { print_error_errno("faidx", "failed to write output"); return EXIT_FAILURE; } } return EXIT_SUCCESS; } static int write_output(faidx_t *faid, FILE *file, const char *name, const int ignore, const hts_pos_t length, const int rev, const char *pos_strand_name, const char *neg_strand_name, enum fai_format_options format) { hts_pos_t seq_len, wrap_len = length; if (wrap_len < 0) wrap_len = fai_line_length(faid, name); if (wrap_len <= 0) wrap_len = HTS_POS_MAX; char *seq = fai_fetch64(faid, name, &seq_len); if (format == FAI_FASTA) { fprintf(file, ">%s%s\n", name, rev ? neg_strand_name : pos_strand_name); } else { fprintf(file, "@%s%s\n", name, rev ? neg_strand_name : pos_strand_name); } if (rev && seq_len > 0) { reverse_complement(seq, seq_len); } if (write_line(faid, file, seq, name, ignore, wrap_len, seq_len) == EXIT_FAILURE) { free(seq); return EXIT_FAILURE; } free(seq); if (format == FAI_FASTQ) { fprintf(file, "+\n"); char *qual = fai_fetchqual64(faid, name, &seq_len); if (rev && seq_len > 0) { reverse(qual, seq_len); } if (write_line(faid, file, qual, name, ignore, wrap_len, seq_len) == EXIT_FAILURE) { free(qual); return EXIT_FAILURE; } free(qual); } return EXIT_SUCCESS; } static int read_regions_from_file(faidx_t *faid, hFILE *in_file, FILE *file, const int ignore, const hts_pos_t length, const int rev, const char *pos_strand_name, const char *neg_strand_name, enum fai_format_options format) { kstring_t line = {0, 0, NULL}; int ret = EXIT_FAILURE; while (line.l = 0, kgetline(&line, (kgets_func *)hgets, in_file) >= 0) { if ((ret = write_output(faid, file, line.s, ignore, length, rev, pos_strand_name, neg_strand_name, format)) == EXIT_FAILURE) { break; } } free(line.s); return ret; } static int usage(FILE *fp, enum fai_format_options format, int exit_status) { char *tool, *file_type, *index_name; if (format == FAI_FASTA) { tool = "faidx "; file_type = "FASTA"; index_name = "file.fa"; } else { tool = "fqidx "; file_type = "FASTQ"; index_name = "file.fq"; } fprintf(fp, "Usage: samtools %s [ [...]]\n", tool); fprintf(fp, "Option: \n" " -o, --output FILE Write %s to file.\n" " -n, --length INT Length of %s sequence line. [60]\n" " -c, --continue Continue after trying to retrieve missing region.\n" " -r, --region-file FILE File of regions. Format is chr:from-to. One per line.\n" " -i, --reverse-complement Reverse complement sequences.\n" " --mark-strand TYPE Add strand indicator to sequence name\n" " TYPE = rc for /rc on negative strand (default)\n" " no for no strand indicator\n" " sign for (+) / (-)\n" " custom,, for custom indicator\n" " --fai-idx FILE name of the index file (default %s.fai).\n" " --gzi-idx FILE name of compressed file index (default %s.gz.gzi).\n", file_type, file_type, index_name, index_name); if (format == FAI_FASTA) { fprintf(fp, " -f, --fastq File and index in FASTQ format.\n"); } fprintf(fp, " -h, --help This message.\n"); return exit_status; } int faidx_core(int argc, char *argv[], enum fai_format_options format) { int c, ignore_error = 0, rev = 0; hts_pos_t line_len = DEFAULT_FASTA_LINE_LEN ;/* fasta line len */ char* output_file = NULL; /* output file (default is stdout ) */ char *region_file = NULL; // list of regions from file, one per line char *pos_strand_name = ""; // Extension to add to name for +ve strand char *neg_strand_name = "/rc"; // Extension to add to name for -ve strand char *strand_names = NULL; // Used for custom strand annotation char *fai_name = NULL; // specified index name char *gzi_name = NULL; // specified compressed index name FILE* file_out = stdout;/* output stream */ static const struct option lopts[] = { { "output", required_argument, NULL, 'o' }, { "help", no_argument, NULL, 'h' }, { "length", required_argument, NULL, 'n' }, { "continue", no_argument, NULL, 'c' }, { "region-file", required_argument, NULL, 'r' }, { "fastq", no_argument, NULL, 'f' }, { "reverse-complement", no_argument, NULL, 'i' }, { "mark-strand", required_argument, NULL, 1000 }, { "fai-idx", required_argument, NULL, 1001 }, { "gzi-idx", required_argument, NULL, 1002 }, { NULL, 0, NULL, 0 } }; while ((c = getopt_long(argc, argv, "ho:n:cr:fi", lopts, NULL)) >= 0) { switch (c) { case 'o': output_file = optarg; break; case 'n': line_len = strtol(optarg, NULL, 10); if (line_len < 0) { fprintf(stderr,"[faidx] bad line length '%s', using default:%d\n",optarg,ABS(DEFAULT_FASTA_LINE_LEN)); line_len= ABS(DEFAULT_FASTA_LINE_LEN); } break; case 'c': ignore_error = 1; break; case 'r': region_file = optarg; break; case 'f': format = FAI_FASTQ; break; case 'i': rev = 1; break; case '?': return usage(stderr, format, EXIT_FAILURE); case 'h': return usage(stdout, format, EXIT_SUCCESS); case 1000: if (strcmp(optarg, "no") == 0) { pos_strand_name = neg_strand_name = ""; } else if (strcmp(optarg, "sign") == 0) { pos_strand_name = "(+)"; neg_strand_name = "(-)"; } else if (strcmp(optarg, "rc") == 0) { pos_strand_name = ""; neg_strand_name = "/rc"; } else if (strncmp(optarg, "custom,", 7) == 0) { size_t len = strlen(optarg + 7); size_t comma = strcspn(optarg + 7, ","); free(strand_names); strand_names = pos_strand_name = malloc(len + 2); if (!strand_names) { fprintf(stderr, "[faidx] Out of memory\n"); return EXIT_FAILURE; } neg_strand_name = pos_strand_name + comma + 1; memcpy(pos_strand_name, optarg + 7, comma); pos_strand_name[comma] = '\0'; if (comma < len) memcpy(neg_strand_name, optarg + 7 + comma + 1, len - comma); neg_strand_name[len - comma] = '\0'; } else { fprintf(stderr, "[faidx] Unknown --mark-strand option \"%s\"\n", optarg); return usage(stderr, format, EXIT_FAILURE); } break; case 1001: fai_name = optarg; break; case 1002: gzi_name = optarg; break; default: break; } } if ( argc==optind ) return usage(stdout, format, EXIT_SUCCESS); if (optind+1 == argc && !region_file) { if (output_file && !fai_name) fai_name = output_file; if (fai_build3(argv[optind], fai_name, gzi_name) != 0) { if (fai_name) fprintf(stderr, "[faidx] Could not build fai index %s", fai_name); else fprintf(stderr, "[faidx] Could not build fai index %s.fai", argv[optind]); if (gzi_name) fprintf(stderr, " or compressed index %s\n", gzi_name); else fprintf(stderr, "\n"); return EXIT_FAILURE; } return 0; } faidx_t *fai = fai_load3_format(argv[optind], fai_name, gzi_name, FAI_CREATE, format); if (!fai) { if (fai_name) fprintf(stderr, "[faidx] Could not load fai index %s", fai_name); else fprintf(stderr, "[faidx] Could not build fai index %s.fai", argv[optind]); if (gzi_name) fprintf(stderr, " or compressed index %s\n", gzi_name); else fprintf(stderr, "\n"); return EXIT_FAILURE; } /** output file provided by user */ if( output_file != NULL ) { if( strcmp( output_file, argv[optind] ) == 0 ) { fprintf(stderr,"[faidx] Same input/output : %s\n", output_file); return EXIT_FAILURE; } file_out = fopen( output_file, "w" ); if( file_out == NULL) { fprintf(stderr,"[faidx] Cannot open \"%s\" for writing :%s.\n", output_file, strerror(errno) ); return EXIT_FAILURE; } } int exit_status = EXIT_SUCCESS; if (region_file) { hFILE *rf; if ((rf = hopen(region_file, "r"))) { exit_status = read_regions_from_file(fai, rf, file_out, ignore_error, line_len, rev, pos_strand_name, neg_strand_name, format); if (hclose(rf) != 0) { fprintf(stderr, "[faidx] Warning: failed to close %s", region_file); } } else { fprintf(stderr, "[faidx] Failed to open \"%s\" for reading.\n", region_file); exit_status = EXIT_FAILURE; } } while ( ++optind