#!/usr/bin/env python # Copyright (c) 2005 Gavin E. Crooks # # This software is distributed under the MIT Open Source License. # # # Permission is hereby granted, free of charge, to any person obtaining a # copy of this software and associated documentation files (the "Software"), # to deal in the Software without restriction, including without limitation # the rights to use, copy, modify, merge, publish, distribute, sublicense, # and/or sell copies of the Software, and to permit persons to whom the # Software is furnished to do so, subject to the following conditions: # # The above copyright notice and this permission notice shall be included # in all copies or substantial portions of the Software. # # THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR # IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, # FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE # AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER # LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, # OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN # THE SOFTWARE. # """Read and write sequence information in IntelliGenetics format. A sequence file in IG format can contain several sequences, each consisting of a number of comment lines that must begin with a semicolon (";"), a line with the sequence name and the sequence itself terminated with the termination character '1' for linear or '2' for circular sequences. The termination character is defacto optional. --- Example IG File --- ;H.sapiens fau mRNA, 518 bases HSFAU ttcctctttctcgactccatcttcgcggtagctgggaccgccgttcagtc actcttaagtcttttgtaattctggctttctctaataaaaaagccactta gttcagtcaaaaaaaaaa1 ;H.sapiens fau 1 gene, 2016 bases HSFAU1 ctaccattttccctctcgattctatatgtacactcgggacaagttctcct gatcgaaaacggcaaaactaaggccccaagtaggaatgccttagttttcg gggttaacaatgattaacactgagcctcacacccacgcgatgccctcagc tcctcgctcagcgctctcaccaacagccgtagcccgcagccccgctggac accggttctccatccccgcagcgtagcccggaacatggtagctgccatct ttacctgctacgccagccttctgtgcgcgcaactgtctggtcccgcccc2 """ from typing import Iterator, List, Optional, TextIO from ..seq import Alphabet, Seq, SeqList from ..utils import remove_whitespace names = ( "intelligenetics", "ig", "stanford", ) extensions = ("ig",) example = """ ;H.sapiens fau mRNA, 518 bases HSFAU ttcctctttctcgactccatcttcgcggtagctgggaccgccgttcagtc actcttaagtcttttgtaattctggctttctctaataaaaaagccactta gttcagtcaaaaaaaaaa1 ;H.sapiens fau 1 gene, 2016 bases HSFAU1 ctaccattttccctctcgattctatatgtacactcgggacaagttctcct gatcgaaaacggcaaaactaaggccccaagtaggaatgccttagttttcg gggttaacaatgattaacactgagcctcacacccacgcgatgccctcagc tcctcgctcagcgctctcaccaacagccgtagcccgcagccccgctggac accggttctccatccccgcagcgtagcccggaacatggtagctgccatct ttacctgctacgccagccttctgtgcgcgcaactgtctggtcccgcccc2 """ def read(fin: TextIO, alphabet: Alphabet = None) -> SeqList: """Read and parse an IG file. Args: fin -- A stream or file to read alphabet -- The expected alphabet of the data, if given Returns: SeqList -- A list of sequences Raises: ValueError -- If the file is unparsable """ seqs = [s for s in iterseq(fin, alphabet)] return SeqList(seqs) def iterseq(fin: TextIO, alphabet: Alphabet = None) -> Iterator[Seq]: """Parse an IG file and generate sequences. Args: fin -- A stream or file to read alphabet -- The expected alphabet of the data, if given Yields: Seq -- One alphabetic sequence at a time. Raises: ValueError -- If the file is unparsable """ alphabet = Alphabet(alphabet) seqs: list = [] header: list = [] start_lineno = -1 name = None def build_seq( seqs: List[str], alphabet: Alphabet, name: Optional[str], comments: List[str], lineno: int, ) -> Seq: try: desc = "\n".join(comments) s = Seq("".join(seqs), alphabet, name=name, description=desc) except ValueError: raise ValueError( "Parse failed with sequence starting at line %d: " "Character not in alphabet: %s" % (lineno, alphabet) ) return s for lineno, line in enumerate(fin): line = line.strip() if line == "": continue if line.startswith(";"): if seqs: # end of sequence yield build_seq(seqs, alphabet, name, header, start_lineno) header = [] seqs = [] name = None header.append(line[1:]) start_lineno = lineno elif not name: name = line elif line[-1] == "1" or line[-1] == "2": # End of sequence seqs.append(remove_whitespace(line[0:-1])) yield build_seq(seqs, alphabet, name, header, start_lineno) header = [] seqs = [] name = None else: seqs.append(remove_whitespace(line)) if seqs: yield build_seq(seqs, alphabet, name, header, start_lineno) return def write(fout: TextIO, seqs: SeqList) -> None: """Write an IG file. Args: fout -- A writable stream. seqs -- A list of Seq's Raises: ValueError -- If a sequence is missing a name """ for s in seqs: writeseq(fout, s) def writeseq(fout: TextIO, seq: Seq) -> None: """Write a single sequence in IG format. Args: fout -- A writable stream. seq -- A Seq instance Raises: ValueError -- If a sequence is missing a name """ desc = seq.description or "" # We prepend ';' to each line for h in desc.splitlines(): print(";" + h, file=fout) if not seq.name: raise ValueError("Write failed with missing sequence name: %s" % str(seq)) print(seq.name, file=fout) L = len(seq) line_length = 80 for n in range(1 + L // line_length): print(seq[n * line_length : (n + 1) * line_length], file=fout) print(file=fout)