/* * BioJava development code * * This code may be freely distributed and modified under the * terms of the GNU Lesser General Public Licence. This should * be distributed with the code. If you do not have a copy, * see: * * http://www.gnu.org/copyleft/lesser.html * * Copyright for this code is held jointly by the individual * authors. These should be listed in @author doc comments. * * For more information on the BioJava project and its aims, * or to join the biojava-l mailing list, visit the home page * at: * * http://www.biojava.org/ * */ package seq; import java.io.*; import org.biojava.bio.seq.SequenceTools; import org.biojavax.Namespace; import org.biojavax.RichObjectFactory; import org.biojavax.bio.seq.RichSequence; import org.biojavax.bio.seq.RichSequenceIterator; /** * Demo of reverse complementing a fasta DNA file * @author Matthew Pocock * @author Mark Schreiber */ public class ReverseComplement { /** * Run the program * @param args a dna fasta file */ public static void main(String[] args) throws Exception { if(args.length < 1) { System.err.println("Use: seq.ReverseComplement inFile"); System.exit(1); } BufferedReader seqIn = new BufferedReader( new FileReader( new File(args[0]) ) ); OutputStream seqOut = System.out; Namespace ns = RichObjectFactory.getDefaultNamespace(); for(RichSequenceIterator si = RichSequence.IOTools.readFastaDNA(seqIn, ns); si.hasNext(); ) { RichSequence seq = si.nextRichSequence(); RichSequence rev = RichSequence.Tools.enrich(SequenceTools.reverseComplement(seq)); RichSequence.IOTools.writeFasta(seqOut, rev, ns); } } }