LOCUS PI76KSIV 4098 bp DNA Circular SYN 3-JUN-1997 DEFINITION Plasmid pI176KSiv - internal PCR standard ACCESSION MD88001 KEYWORDS . SOURCE derived from recombinant construct pI176KS. ORGANISM synthetic REFERENCE 1 (sites) AUTHORS Fristensky, Brian W TITLE Untitled JOURNAL Unpublished (1997) Plant Science, University of Manitoba STANDARD full automatic COMMENT This clone was made Mary DePauw in Brian Fristensky's lab, Experiment MD88. It contains the 341bp pUC18/Sau3A1 fragment inserted at the BglII site within the cDNA. Amplification using primers flanking this insertion gives a PCR fragment 341bp longer than that generated in RT-PCR from mRNA. Corresponding Address for Citation 1: Brian W Fristensky University of Manitoba Winnipeg, MB R3T 2N2 Canada Phone: 204-474-6085 Fax: 204-261-5732 Email: frist@cc.umanitoba.ca FEATURES LOCATION/QUALIFIERS misc_feature M18249:1..355 /evidence= EXPERIMENTAL /label=PR10.2_cDNA_left misc_feature L08752:complement(1369..1709) /function= "vector" /evidence= EXPERIMENTAL /standard_name= "SYNPUC18V" /label=pUC18_frag /note= "concatenated Sau3A1 digest fragments" misc_feature M18249:356..728 /evidence= EXPERIMENTAL /label=PR10.2_cDNA_right unsure "aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa" /label=polyA_tail /note= "About 50bp but precise length unknown" misc_feature "tgcagg" /evidence= EXPERIMENTAL /label=Pst_to_Sal /note= "pUC9 polylinker, from T-tailed PstI site to SalI" misc_feature X52331:join(735..2958,1..719) /function= "vector" /evidence= EXPERIMENTAL /standard_name= "Bluescript KSm13+" /label=KSm13_H3_Sal /note= "Bluescript KSm13+/HindIII/SalI cut" misc_feature "agcttggctgcacccccccccccccccccc" /evidence= EXPERIMENTAL /label=polyC-tail /note= "pUC9 polylinker from HindIII to C-tailed PstI site" source join(PR10.2_cDNA_left,pUC18_frag,PR10.2_cDNA_right, polyA_tail,Pst_to_Sal,KSm13_H3_Sal,polyC-tail) /organism="Escherichia coli" /plasmid="pI176KSiv" /evidence= EXPERIMENTAL /label=pI176KSiv BASE COUNT 1088 a 980 c 981 g 1049 t ORIGIN 1 tacacaacta ggcaagcaat ttcttagttc tttctcacac tttagcaata taaaacaata 61 tcatcatggg tgtttttaat gttgaagatg aaatcacttc tgttgtagca cctgctatac 121 tctacaaagc tctagttaca gatgctgaca ctcttactcc aaaggttatt gatgccatca 181 aaagtatcga aattgttgaa ggaaacggag gtgctggaac catcaagaaa ctcactttcg 241 ttgaagatgg tgaaaccaaa catgtgttgc acaaagtgga gttagtagat gttgctaact 301 tggcttacaa ctatagcata gttggtggtg ttggatttcc agacacagtt gagaagatct 361 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg 421 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc 481 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa 541 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc 601 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt 661 cgtttggtat ggcttcattc agctccggtt cccaacgatc tcatttgagg ctaaactgtc 721 tgcaggacca aatggaggat ccattgcaaa gctgagtgtg aaatattaca caaaaggtga 781 tgctgctgct cctactgaag agcaactcaa gagtgacaaa gctaagggtg atggtctttt 841 caaggctctt gagcgttact gtttggctca tcctgattac aactaaacta tataatcatc 901 aacaagtgtg tgttgttatg tatactatat aatcatcatg tgtgcttaat ttggctgcca 961 atgtaatccc cctgttttgt tttttccctt tcccttttct ttgtggacta aaagtgtgag 1021 attgtaagtc atgtatacct ctccttcaat aaattataat catttatata aaaaaaaaaa 1081 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaat gcaggtcgac ctcgaggggg 1141 ggcccggtac ccagcttttg ttccctttag tgagggttaa tttcgagctt ggcgtaatca 1201 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga 1261 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt 1321 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga 1381 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc 1441 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg 1501 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc 1561 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc 1621 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga 1681 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc 1741 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat 1801 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg 1861 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc 1921 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga 1981 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact 2041 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt 2101 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag 2161 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg 2221 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa 2281 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata 2341 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg 2401 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata 2461 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg 2521 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct 2581 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt 2641 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc 2701 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga 2761 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt 2821 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc 2881 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa 2941 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca 3001 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca 3061 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct 3121 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc 3181 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa 3241 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt 3301 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctaaattg 3361 taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta 3421 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt 3481 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca 3541 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa 3601 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat 3661 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag 3721 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg 3781 ccgcgcttaa tgcgccgcta cagggcgcgt cccattcgcc attcaggctg cgcaactgtt 3841 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg 3901 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga 3961 cggccagtga attgtaatac gactcactat agggcgaatt ggagctccac cgcggtggcg 4021 gccgctctag aactagtgga tcccccgggc tgcaggaatt cgatatcaag cttggctgca 4081 cccccccccc cccccccc //