LOCUS PI49KSV 4258 bp DNA Circular SYN 2-JUN-1997 DEFINITION Plasmid pI49KSv - internal PCR standard ACCESSION MD8800 KEYWORDS . SOURCE derived from recombinant construct pI49KS. ORGANISM synthetic REFERENCE 1 (sites) AUTHORS Fristensky, Brian W TITLE Untitled JOURNAL Unpublished (1997) Plant Science, University of Manitoba STANDARD full automatic COMMENT This clone was made Mary DePauw in Brian Fristensky's lab, Experiment MD88. It contains five pUC18/Sau3A1 fragments inserted at the BglII site within the cDNA. Amplification using primers flanking this insertion gives a PCR fragment 495bp longer than that generated in RT-PCR from mRNA. Corresponding Address for Citation 1: Brian W Fristensky University of Manitoba Winnipeg, MB R3T 2N2 Canada Phone: 204-474-6085 Fax: 204-261-5732 Email: frist@cc.umanitoba.ca FEATURES LOCATION/QUALIFIERS misc_feature X13383:1..354 /evidence= EXPERIMENTAL /label=drr49a_cDNA_left misc_feature L08752:complement(1710..1814) /function= "vector" /evidence= EXPERIMENTAL /standard_name= "SYNPUC18V" /label=pUC18_105 /note= "concatenated Sau3A1 digest fragments" misc_feature L08752:join(1043..1300,1301..1346, 1823..1900,1901..1908) /function= "vector" /evidence= EXPERIMENTAL /standard_name= "SYNPUC18V" /label=pUC18_frags /note= "concatenated Sau3A1 digest fragments" misc_feature X13383:355..734 /evidence= EXPERIMENTAL /label=drr49a_cDNA_right unsure "aaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa" /label=polyA_tail /note= "About 50bp but precise length unknown" misc_feature "tgcagg" /evidence= EXPERIMENTAL /label=Pst_to_Sal /note= "pUC9 polylinker, from T-tailed PstI site to SalI" misc_feature X52331:join(735..2958,1..719) /function= "vector" /evidence= EXPERIMENTAL /standard_name= "Bluescript KSm13+" /label=KSm13_H3_Sal /note= "Bluescript KSm13+/HindIII/SalI cut" misc_feature "agcttggctgcacccccccccccccccccc" /evidence= EXPERIMENTAL /label=polyC-tail /note= "pUC9 polylinker from HindIII to C-tailed PstI site" source join(drr49a_cDNA_left,pUC18_105,pUC18_frags, drr49a_cDNA_right,polyA_tail, Pst_to_Sal,KSm13_H3_Sal,polyC-tail) /organism="Escherichia coli" /plasmid="pI49KSv" /evidence= EXPERIMENTAL /label=pI49KSv BASE COUNT 1165 a 986 c 1003 g 1104 t ORIGIN 1 acacaactag gcaagcaatt tcttagttct ttctcacact ttagcattat aaaacattat 61 catcatgggt gtttttaatg ttgaagatga aatcacttct gttgtagcac ctgctatact 121 ctacaaagct ctagttacag atgctgataa ccttactcca aaggttattg atgccatcaa 181 aagtatcgaa attgttgaag gaaacggtgg tgctggaacc atcaaaaaac tcactttcgt 241 tgaagatggt gaaaccaaac atgtgttgca caaagtggag ttagtagatg ttgctaactt 301 ggcttacaac tatagcatag ttggtggtgt tggatttcca gacacagttg agaagatcct 361 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga 421 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atccttgaga gttttcgccc 481 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc 541 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt 601 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt 661 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat 721 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcctt tttgataatc 781 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa 841 agatcaaagg atctcattcg aggctaaact gtctgcagga ccaaatggag gatccattgc 901 aaagctgagt gtgaaatact tcacaaaagg tgatgctgct cctagtgaag agcaactcaa 961 gactgacaaa gctaaggggg atggtctttt caaggctctt gagggttact gtttggctca 1021 tcctgattac aactaaacta tataatcatc aacaagtgtg ttgttatgta tactatataa 1081 tcatcctgtg tgcttaattt ggctgccaat gtaattttct gttttgtttt tttccttttc 1141 ttttgttgtg gaactaaaag tgtgagattg taagtcatgt atacctctcc ttcaataaat 1201 tataataata atttatatat atctttttta aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 1261 aaaaaaaaaa aaaaaaaaat gcaggtcgac ctcgaggggg ggcccggtac ccagcttttg 1321 ttccctttag tgagggttaa tttcgagctt ggcgtaatca tggtcatagc tgtttcctgt 1381 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa 1441 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc 1501 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag 1561 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt 1621 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga 1681 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg 1741 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa 1801 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt 1861 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct 1921 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct 1981 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc 2041 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt 2101 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc 2161 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat 2221 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa 2281 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa 2341 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga 2401 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct 2461 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga 2521 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc 2581 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg 2641 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat 2701 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat 2761 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg 2821 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc 2881 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa 2941 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc 3001 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt 3061 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag 3121 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt 3181 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag 3241 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac 3301 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc 3361 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca 3421 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg 3481 ggttccgcgc acatttcccc gaaaagtgcc acctaaattg taagcgttaa tattttgtta 3541 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc 3601 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg 3661 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat 3721 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc 3781 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag 3841 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg 3901 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta 3961 cagggcgcgt cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg 4021 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg 4081 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga attgtaatac 4141 gactcactat agggcgaatt ggagctccac cgcggtggcg gccgctctag aactagtgga 4201 tcccccgggc tgcaggaatt cgatatcaag cttggctgca cccccccccc cccccccc //