REBASE version 211 striderc.211 =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= REBASE, The Restriction Enzyme Database http://rebase.neb.com Copyright (c) Dr. Richard J. Roberts, 2012. All rights reserved. =-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-= Rich Roberts Oct 30 2012 AanI,tta/taa, #AarI,cacctgc,4,8, AasI,gacnnnn/nngtc, AatII,gacgt/c, AbsI,cc/tcgagg, AccI,gt/mkac, AccII,cg/cg, AccIII,t/ccgga, Acc16I,tgc/gca, #Acc36I,acctgc,4,8, Acc65I,g/gtacc, AccB1I,g/gyrcc, AccB7I,ccannnn/ntgg, #AccBSI,ccgctc,-3,-3, #AceIII,cagctc,7,11, #AciI,ccgc,-3,-1, AclI,aa/cgtt, #AclWI,ggatc,4,5, AcoI,y/ggccr, AcsI,r/aatty, #AcuI,ctgaag,16,14, AcvI,cac/gtg, AcyI,gr/cgyc, AdeI,cacnnn/gtg, AfaI,gt/ac, AfeI,agc/gct, AfiI,ccnnnnn/nngg, AflII,c/ttaag, AflIII,a/crygt, AgeI,a/ccggt, AgsI,tts/aa, AhaIII,ttt/aaa, AhdI,gacnnn/nngtc, AhlI,a/ctagt, #AjiI,cacgtc,-3,-3, AjnI,/ccwgg, #AjuI,gaannnnnnnttgg,11,6, AleI,cacnn/nngtg, #AlfI,gcannnnnntgc,12,10, #AloI,gaacnnnnnntcc,12,7, AluI,ag/ct, AluBI,ag/ct, #AlwI,ggatc,4,5, Alw21I,gwgcw/c, #Alw26I,gtctc,1,5, Alw44I,g/tgcac, AlwNI,cagnnn/ctg, Ama87I,c/ycgrg, Aor13HI,t/ccgga, Aor51HI,agc/gct, AoxI,/ggcc, ApaI,gggcc/c, ApaBI,gcannnnn/tgc, ApaLI,g/tgcac, ApeKI,g/cwgc, ApoI,r/aatty, #ApyPI,atcgac,20,18, #AquII,gccgnac,20,18, #AquIII,gaggag,20,18, #AquIV,grggaag,19,17, #ArsI,gacnnnnnnttyg,11,6, AscI,gg/cgcgcc, AseI,at/taat, Asi256I,g/atc, AsiGI,a/ccggt, AsiSI,gcgat/cgc, AspI,gacn/nngtc, Asp700I,gaann/nnttc, Asp718I,g/gtacc, AspA2I,c/ctagg, #AspBHI,yscns,8,12, AspEI,gacnnn/nngtc, AspLEI,gcg/c, AspS9I,g/gncc, AssI,agt/act, AsuI,g/gncc, AsuII,tt/cgaa, AsuC2I,cc/sgg, #AsuHPI,ggtga,8,7, AsuNHI,g/ctagc, AvaI,c/ycgrg, AvaII,g/gwcc, AviII,tgc/gca, AvrII,c/ctagg, AxyI,cc/tnagg, #BaeI,acnnnngtayc,12,7, BaeGI,gkgcm/c, BalI,tgg/cca, BamHI,g/gatcc, BanI,g/gyrcc, BanII,grgcy/c, #BarI,gaagnnnnnntac,12,7, BasI,ccannnn/ntgg, #BauI,cacgag,-5,-1, BbeI,ggcgc/c, #Bbr7I,gaagac,7,11, BbrPI,cac/gtg, #BbsI,gaagac,2,6, #BbvI,gcagc,8,12, #BbvII,gaagac,2,6, Bbv12I,gwgcw/c, #BbvCI,cctcagc,-5,-2, #BccI,ccatc,4,5, #Bce83I,cttgag,16,14, #BceAI,acggc,12,14, #R1.BceSIV,gcagc,9,11, #BcefI,acggc,12,13, #BcgI,cgannnnnntgc,12,10, BciT130I,cc/wgg, #BciVI,gtatcc,6,5, BclI,t/gatca, BcnI,cc/sgg, #BcoDI,gtctc,1,5, BcuI,a/ctagt, #BdaI,tgannnnnntca,12,10, BetI,w/ccggw, BfaI,c/tag, #BfiI,actggg,5,4, BfmI,c/tryag, BfoI,rgcgc/y, BfrI,c/ttaag, #BfuI,gtatcc,6,5, #BfuAI,acctgc,4,8, BfuCI,/gatc, BglI,gccnnnn/nggc, BglII,a/gatct, #BinI,ggatc,4,5, BisI,gc/ngc, BlnI,c/ctagg, BlpI,gc/tnagc, BlsI,gcn/gc, BmcAI,agt/act, Bme18I,g/gwcc, Bme1390I,cc/ngg, BmeRI,gacnnn/nngtc, BmeT110I,c/ycgrg, #BmgBI,cacgtc,-3,-3, BmgT120I,gg/ncc, BmiI,ggn/ncc, #BmrI,actggg,5,4, BmrFI,cc/ngg, #BmsI,gcatc,5,9, BmtI,gctag/c, #BmuI,actggg,5,4, BoxI,gacnn/nngtc, #BpiI,gaagac,2,6, #BplI,gagnnnnnctc,13,8, #BpmI,ctggag,16,14, #Bpu10I,cctnagc,-5,-2, Bpu14I,tt/cgaa, Bpu1102I,gc/tnagc, #BpuAI,gaagac,2,6, #BpuEI,cttgag,16,14, BpuMI,cc/sgg, BpvUI,cgat/cg, #BsaI,ggtctc,1,5, Bsa29I,at/cgat, BsaAI,yac/gtr, BsaBI,gatnn/nnatc, BsaHI,gr/cgyc, BsaJI,c/cnngg, BsaWI,w/ccggw, #BsaXI,acnnnnnctcc,10,7, #BsbI,caacac,21,19, Bsc4I,ccnnnnn/nngg, #BscAI,gcatc,4,6, #Bse1I,actgg,1,-1, Bse8I,gatnn/nnatc, Bse21I,cc/tnagg, Bse118I,r/ccggy, BseAI,t/ccgga, BseBI,cc/wgg, BseCI,at/cgat, BseDI,c/cnngg, #Bse3DI,gcaatg,2,0, #BseGI,ggatg,2,0, BseJI,gatnn/nnatc, BseLI,ccnnnnn/nngg, #BseMI,gcaatg,2,0, #BseMII,ctcag,10,8, #BseNI,actgg,1,-1, BsePI,g/cgcgc, #BseRI,gaggag,10,8, BseSI,gkgcm/c, #BseXI,gcagc,8,12, BseX3I,c/ggccg, #BseYI,cccagc,-5,-1, #BsgI,gtgcag,16,14, Bsh1236I,cg/cg, Bsh1285I,cgry/cg, BshFI,gg/cc, BshNI,g/gyrcc, BshTI,a/ccggt, BshVI,at/cgat, #BsiI,cacgag,-5,-1, BsiEI,cgry/cg, BsiHKAI,gwgcw/c, BsiHKCI,c/ycgrg, BsiSI,c/cgg, BsiWI,c/gtacg, BsiYI,ccnnnnn/nngg, BslI,ccnnnnn/nngg, #BslFI,gggac,10,14, #BsmI,gaatgc,1,-1, #BsmAI,gtctc,1,5, #BsmBI,cgtctc,1,5, #BsmFI,gggac,10,14, BsnI,gg/cc, #Bso31I,ggtctc,1,5, BsoBI,c/ycgrg, Bsp13I,t/ccgga, Bsp19I,c/catgg, #Bsp24I,gacnnnnnntgg,12,7, Bsp68I,tcg/cga, Bsp119I,tt/cgaa, Bsp120I,g/ggccc, Bsp143I,/gatc, Bsp1286I,gdgch/c, Bsp1407I,t/gtaca, Bsp1720I,gc/tnagc, #BspACI,ccgc,-3,-1, #BspCNI,ctcag,9,7, BspDI,at/cgat, #BspD6I,gactc,4,6, BspEI,t/ccgga, BspFNI,cg/cg, BspHI,t/catga, BspLI,ggn/ncc, BspLU11I,a/catgt, #BspMI,acctgc,4,8, BspMII,t/ccgga, BspOI,gctag/c, #BspPI,ggatc,4,5, #BspQI,gctcttc,1,4, BspTI,c/ttaag, BspT104I,tt/cgaa, BspT107I,g/gyrcc, #BsrI,actgg,1,-1, #BsrBI,ccgctc,-3,-3, #BsrDI,gcaatg,2,0, BsrFI,r/ccggy, BsrGI,t/gtaca, #BsrSI,actgg,1,-1, BssAI,r/ccggy, BssECI,c/cnngg, BssHII,g/cgcgc, BssKI,/ccngg, BssMI,/gatc, BssNI,gr/cgyc, BssNAI,gta/tac, #BssSI,cacgag,-5,-1, BssT1I,c/cwwgg, #Bst6I,ctcttc,1,4, Bst1107I,gta/tac, BstACI,gr/cgyc, BstAFI,c/ttaag, BstAPI,gcannnn/ntgc, BstAUI,t/gtaca, BstBI,tt/cgaa, #Bst2BI,cacgag,-5,-1, BstBAI,yac/gtr, Bst4CI,acn/gt, BstC8I,gcn/ngc, BstDEI,c/tnag, BstDSI,c/crygg, BstEII,g/gtnacc, BstENI,cctnn/nnnagg, #BstF5I,ggatg,2,0, BstFNI,cg/cg, BstH2I,rgcgc/y, BstHHI,gcg/c, BstKTI,gat/c, #BstMAI,gtctc,1,5, BstMBI,/gatc, BstMCI,cgry/cg, BstMWI,gcnnnnn/nngc, BstNI,cc/wgg, BstNSI,rcatg/y, BstOI,cc/wgg, BstPI,g/gtnacc, BstPAI,gacnn/nngtc, BstSCI,/ccngg, BstSFI,c/tryag, BstSLI,gkgcm/c, BstSNI,tac/gta, BstUI,cg/cg, Bst2UI,cc/wgg, #BstV1I,gcagc,8,12, #BstV2I,gaagac,2,6, BstXI,ccannnnn/ntgg, BstX2I,r/gatcy, BstYI,r/gatcy, BstZI,c/ggccg, BstZ17I,gta/tac, #BsuI,gtatcc,6,5, Bsu15I,at/cgat, Bsu36I,cc/tnagg, BsuRI,gg/cc, BtgI,c/crygg, #BtgZI,gcgatg,10,14, BthCI,gcng/c, #BtrI,cacgtc,-3,-3, #BtsI,gcagtg,2,0, #BtsCI,ggatg,2,0, #BtsIMutI,cagtg,2,0, BtuMI,tcg/cga, #BveI,acctgc,4,8, Cac8I,gcn/ngc, CaiI,cagnnn/ctg, CauII,cc/sgg, #CchII,ggarga,11,9, #CchIII,cccaag,20,18, CciI,t/catga, CciNI,gc/ggccgc, #CdiI,catcg,-1,-1, #CdpI,gcggag,20,18, CelII,gc/tnagc, CfoI,gcg/c, CfrI,y/ggccr, Cfr9I,c/ccggg, Cfr10I,r/ccggy, Cfr13I,g/gncc, Cfr42I,ccgc/gg, ChaI,gatc/, #CjeI,ccannnnnngt,15,9, #CjeNIII,gkaayg,19,17, #CjePI,ccannnnnnntc,14,8, ClaI,at/cgat, CpoI,cg/gwccg, #CseI,gacgc,5,10, CsiI,a/ccwggt, CspI,cg/gwccg, Csp6I,g/tac, CspAI,a/ccggt, #CspCI,caannnnngtgg,12,10, #CstMI,aaggag,20,18, CviAII,c/atg, CviJI,rg/cy, CviKI-1,rg/cy, CviQI,g/tac, CviRI,tg/ca, DdeI,c/tnag, DinI,ggc/gcc, DpnI,ga/tc, DpnII,/gatc, DraI,ttt/aaa, DraII,rg/gnccy, DraIII,cacnnn/gtg, #DraRI,caagnac,20,18, DrdI,gacnnnn/nngtc, #DrdIV,tacgac,20,18, DriI,gacnnn/nngtc, DsaI,c/crygg, DseDI,gacnnnn/nngtc, EaeI,y/ggccr, EagI,c/ggccg, #Eam1104I,ctcttc,1,4, Eam1105I,gacnnn/nngtc, #EarI,ctcttc,1,4, #EciI,ggcgga,11,9, Ecl136II,gag/ctc, EclXI,c/ggccg, Eco24I,grgcy/c, #Eco31I,ggtctc,1,5, Eco32I,gat/atc, Eco47I,g/gwcc, Eco47III,agc/gct, Eco52I,c/ggccg, #Eco57I,ctgaag,16,14, Eco72I,cac/gtg, Eco81I,cc/tnagg, Eco88I,c/ycgrg, Eco91I,g/gtnacc, Eco105I,tac/gta, Eco130I,c/cwwgg, Eco147I,agg/cct, EcoHI,/ccsgg, EcoICRI,gag/ctc, #Eco57MI,ctgrag,16,14, EcoNI,cctnn/nnnagg, EcoO65I,g/gtnacc, EcoO109I,rg/gnccy, EcoRI,g/aattc, EcoRII,/ccwgg, EcoRV,gat/atc, EcoT14I,c/cwwgg, EcoT22I,atgca/t, EcoT38I,grgcy/c, Eco53kI,gag/ctc, EgeI,ggc/gcc, EheI,ggc/gcc, ErhI,c/cwwgg, EsaBC3I,tc/ga, EspI,gc/tnagc, #Esp3I,cgtctc,1,5, FaeI,catg/, FaiI,ya/tr, #FalI,aagnnnnnctt,13,8, #FaqI,gggac,10,14, FatI,/catg, #FauI,cccgc,4,6, FauNDI,ca/tatg, FbaI,t/gatca, FblI,gt/mkac, FmuI,ggnc/c, FnuDII,cg/cg, Fnu4HI,gc/ngc, #FokI,ggatg,9,13, FriOI,grgcy/c, FseI,ggccgg/cc, FspI,tgc/gca, FspAI,rtgc/gcay, FspBI,c/tag, #FspEI,cc,12,16, Fsp4HI,gc/ngc, #GdiII,cggccr,-5,-1, GlaI,gc/gc, GluI,gc/ngc, #GsaI,cccagc,-1,-5, #GsuI,ctggag,16,14, HaeI,wgg/ccw, HaeII,rgcgc/y, HaeIII,gg/cc, #HaeIV,gaynnnnnrtc,14,9, HapII,c/cgg, #HgaI,gacgc,5,10, HgiAI,gwgcw/c, HgiCI,g/gyrcc, HgiJII,grgcy/c, HhaI,gcg/c, Hin1I,gr/cgyc, Hin1II,catg/, #Hin4I,gaynnnnnvtc,13,8, #Hin4II,ccttc,6,5, Hin6I,g/cgc, HinP1I,g/cgc, HincII,gty/rac, HindII,gty/rac, HindIII,a/agctt, HinfI,g/antc, HpaI,gtt/aac, HpaII,c/cgg, #HphI,ggtga,8,7, Hpy8I,gtn/nac, Hpy99I,cgwcg/, Hpy166II,gtn/nac, Hpy178III,tc/nnga, Hpy188I,tcn/ga, Hpy188III,tc/nnga, #HpyAV,ccttc,6,5, HpyCH4III,acn/gt, HpyCH4IV,a/cgt, HpyCH4V,tg/ca, HpyF3I,c/tnag, HpyF10VI,gcnnnnn/nngc, HpySE526I,a/cgt, Hsp92I,gr/cgyc, Hsp92II,catg/, HspAI,g/cgc, KasI,g/gcgcc, KflI,gg/gwccc, KpnI,ggtac/c, Kpn2I,t/ccgga, KroI,g/ccggc, KspI,ccgc/gg, Ksp22I,t/gatca, #Ksp632I,ctcttc,1,4, KspAI,gtt/aac, Kzo9I,/gatc, #LguI,gctcttc,1,4, LpnI,rgc/gcy, #LpnPI,ccdg,10,14, #Lsp1109I,gcagc,8,12, #LweI,gcatc,5,9, MabI,a/ccwggt, MaeI,c/tag, MaeII,a/cgt, MaeIII,/gtnac, MalI,ga/tc, #MaqI,crttgac,21,19, MauBI,cg/cgcgcg, #MbiI,ccgctc,-3,-3, MboI,/gatc, #MboII,gaaga,8,7, McaTI,gcgc/gc, McrI,cgry/cg, MfeI,c/aattg, MflI,r/gatcy, MhlI,gdgch/c, MlsI,tgg/cca, MluI,a/cgcgt, MluCI,/aatt, MluNI,tgg/cca, #MlyI,gagtc,5,5, Mly113I,gg/cgcc, #MmeI,tccrac,20,18, #MnlI,cctc,7,6, Mph1103I,atgca/t, MreI,cg/ccggcg, MroI,t/ccgga, MroNI,g/ccggc, MroXI,gaann/nnttc, MscI,tgg/cca, MseI,t/taa, MslI,caynn/nnrtg, MspI,c/cgg, Msp20I,tgg/cca, MspA1I,cmg/ckg, MspCI,c/ttaag, #MspJI,cnnr,9,13, MspR9I,cc/ngg, MssI,gttt/aaac, MstI,tgc/gca, MunI,c/aattg, MvaI,cc/wgg, #Mva1269I,gaatgc,1,-1, MvnI,cg/cg, MvrI,cgat/cg, MwoI,gcnnnnn/nngc, NaeI,gcc/ggc, NarI,gg/cgcc, NciI,cc/sgg, NcoI,c/catgg, NdeI,ca/tatg, NdeII,/gatc, #NgoAVIII,gacnnnnntga,13,11, NgoMIV,g/ccggc, NheI,g/ctagc, NlaIII,catg/, NlaIV,ggn/ncc, #NlaCI,catcac,19,17, Nli3877I,cycgr/g, #NmeAIII,gccgag,21,19, #NmeDI,rccggy,7,12, NmuCI,/gtsac, NotI,gc/ggccgc, NruI,tcg/cga, NsbI,tgc/gca, NsiI,atgca/t, NspI,rcatg/y, NspV,tt/cgaa, NspBII,cmg/ckg, OliI,cacnn/nngtg, PabI,gta/c, PacI,ttaat/taa, PaeI,gcatg/c, PaeR7I,c/tcgag, PagI,t/catga, PalAI,gg/cgcgcc, PasI,cc/cwggg, PauI,g/cgcgc, PceI,agg/cct, PciI,a/catgt, #PciSI,gctcttc,1,4, PcsI,wcgnnnn/nnncgw, #PctI,gaatgc,1,-1, PdiI,gcc/ggc, PdmI,gaann/nnttc, PfeI,g/awtc, Pfl23II,c/gtacg, PflFI,gacn/nngtc, PflMI,ccannnn/ntgg, PfoI,t/ccngga, PhoI,gg/cc, PinAI,a/ccggt, #PlaDI,catcag,21,19, #PleI,gagtc,4,5, Ple19I,cgat/cg, PmaCI,cac/gtg, PmeI,gttt/aaac, PmlI,cac/gtg, #PpiI,gaacnnnnnctc,13,8, #PpsI,gagtc,4,5, Ppu10I,a/tgcat, Ppu21I,yac/gtr, PpuMI,rg/gwccy, PscI,a/catgt, PshAI,gacnn/nngtc, PshBI,at/taat, PsiI,tta/taa, Psp03I,ggwc/c, Psp5II,rg/gwccy, Psp6I,/ccwgg, Psp1406I,aa/cgtt, Psp124BI,gagct/c, PspCI,cac/gtg, PspEI,g/gtnacc, PspGI,/ccwgg, PspLI,c/gtacg, PspN4I,ggn/ncc, PspOMI,g/ggccc, #PspOMII,cgcccar,20,18, PspPI,g/gncc, PspPPI,rg/gwccy, #PspPRI,ccycag,15,13, PspXI,vc/tcgagb, #PsrI,gaacnnnnnntac,12,7, PssI,rggnc/cy, PstI,ctgca/g, PstNI,cagnnn/ctg, PsuI,r/gatcy, PsyI,gacn/nngtc, PteI,g/cgcgc, PvuI,cgat/cg, PvuII,cag/ctg, RcaI,t/catga, #RceI,catcgac,20,18, #RdeGBII,acccag,20,18, #RdeGBIII,tgryca,11,9, RgaI,gcgat/cgc, RigI,ggccgg/cc, #RleAI,cccaca,12,9, #RpaI,gtyggag,11,9, #RpaBI,cccgcag,20,18, #RpaB5I,cgrggac,20,18, RruI,tcg/cga, RsaI,gt/ac, RsaNI,g/tac, RseI,caynn/nnrtg, RsrII,cg/gwccg, Rsr2I,cg/gwccg, SacI,gagct/c, SacII,ccgc/gg, SalI,g/tcgac, SanDI,gg/gwccc, #SapI,gctcttc,1,4, SaqAI,t/taa, SatI,gc/ngc, SauI,cc/tnagg, Sau96I,g/gncc, Sau3AI,/gatc, SbfI,cctgca/gg, ScaI,agt/act, #SchI,gagtc,5,5, SciI,ctc/gag, ScrFI,cc/ngg, SdaI,cctgca/gg, #SdeAI,cagrag,21,19, #SdeOSI,gacnnnnrtga,12,10, SduI,gdgch/c, SecI,c/cnngg, SelI,/cgcg, SetI,asst/, SexAI,a/ccwggt, SfaAI,gcgat/cgc, #SfaNI,gcatc,5,9, SfcI,c/tryag, SfeI,c/tryag, SfiI,ggccnnnn/nggcc, SfoI,ggc/gcc, Sfr274I,c/tcgag, Sfr303I,ccgc/gg, SfuI,tt/cgaa, #SgeI,cnng,9,13, SgfI,gcgat/cgc, SgrAI,cr/ccggyg, SgrBI,ccgc/gg, SgrDI,cg/tcgacg, #SgrTI,ccds,10,14, SgsI,gg/cgcgcc, #SimI,gggtc,-3,0, SlaI,c/tcgag, SmaI,ccc/ggg, SmiI,attt/aaat, SmiMI,caynn/nnrtg, SmlI,c/tyrag, SmoI,c/tyrag, #SmuI,cccgc,4,6, SnaBI,tac/gta, SpeI,a/ctagt, SphI,gcatg/c, SplI,c/gtacg, SrfI,gccc/gggc, Sse9I,/aatt, Sse232I,cg/ccggcg, Sse8387I,cctgca/gg, Sse8647I,ag/gwcct, SseBI,agg/cct, #SsiI,ccgc,-3,-1, SspI,aat/att, SspDI,g/gcgcc, #SspD5I,ggtga,8,8, SstI,gagct/c, #SstE37I,cgaagac,20,18, #Sth132I,cccg,4,8, Sth302II,cc/gg, StrI,c/tcgag, #StsI,ggatg,10,14, StuI,agg/cct, StyI,c/cwwgg, StyD4I,/ccngg, SwaI,attt/aaat, TaaI,acn/gt, TaiI,acgt/, TaqI,t/cga, #TaqII,gaccga,11,9, TasI,/aatt, TatI,w/gtacw, TauI,gcsg/c, TfiI,g/awtc, Tru1I,t/taa, Tru9I,t/taa, #TscAI,castg,2,-7, TseI,g/cwgc, TseFI,/gtsac, #TsoI,tarcca,11,9, Tsp45I,/gtsac, Tsp4CI,acn/gt, #TspDTI,atgaa,11,9, TspEI,/aatt, #TspGWI,acgga,11,9, TspMI,c/ccggg, #TspRI,castg,2,-7, #TstI,cacnnnnnntcc,12,7, Tth111I,gacn/nngtc, #Tth111II,caarca,11,9, #UcoMSI,gagctc,5,7, UnbI,/ggncc, Van91I,ccannnn/ntgg, Vha464I,c/ttaag, VneI,g/tgcac, VpaK11AI,/ggwcc, VpaK11BI,g/gwcc, VspI,at/taat, #WviI,cacrag,21,19, XagI,cctnn/nnnagg, XapI,r/aatty, XbaI,t/ctaga, XceI,rcatg/y, XcmI,ccannnnn/nnnntgg, XhoI,c/tcgag, XhoII,r/gatcy, XmaI,c/ccggg, XmaIII,c/ggccg, XmaJI,c/ctagg, XmiI,gt/mkac, XmnI,gaann/nnttc, XspI,c/tag, ZraI,gac/gtc, ZrmI,agt/act, Zsp2I,atgca/t,