#!/usr/bin/env python ################################################ # SPLIT FASTA FILES INTO ONE FILE PER SEQUENCE # # THIS IS OPEN SOURCE CODE (YAY!) # ################################################ # LICENSE # This code is licensed under the Creative Commons 3.0 # Attribution + ShareAlike license - for details see: # http://creativecommons.org/licenses/by-sa/3.0/ # # DATE: February 27th, 2014 # # PROGRAM AUTHOR (PROGRAMMER) # Graham Alvare - home.cc.umanitoba.ca/~alvare # # CO-AUTHORS/ACKNOWLEDGEMENTS # Justin Zhang - for providing me information on the SAM format, # and test data to build and debug this program. # Dr. Brian Fristensky - my work supervisor, and the man who introduced # me to the wonderful field of bioinformatics. # # QUESTIONS & COMMENTS # If you have any questions, please contact me: alvare@cc.umanitoba.ca # I usually get back to people within 1-2 weekdays (weekends, I am slower) # # P.S. Please also let me know of any bugs, or if you have any suggestions. # I am generally happy to help create new tools, or modify my existing # tools to make them more useful. # # Happy usage! import sys, os, os.path, re, collections from Bio import SeqIO if __name__=="__main__": if len(sys.argv) > 1: # process every file specified on the command line for filename in sys.argv[1:]: # Open the FASTA file handle = open(filename, 'rU') # the sequence record number number = 1 # Open output FASTA file for record in SeqIO.parse(handle, 'fasta'): outname = os.path.basename(filename) + ".seq" + str(number) outfile = open(outname, 'w') print >> outfile, "> " + str(record.id) print >> outfile, str(record.seq) outfile.close() number += 1 # Close the input file handle handle.close()