Aligning contigs to reference... Aligning contigs to the reference Filtering alignments... Parsing coords... Loading reference... Loaded [meta_ref_1_Lactobacillus_plantarum_22_55k.fa] Total Regions: 1 Total Region Length: 54999 Analyzing contigs... CONTIG: NODE_6_length_49658_cov_2.58581 (48458bp) Best alignment score: 37170.6 (LEN: 37178, IDY: 99.98), Total number of alignments: 2 Looking for the best set of alignments (out of 2 total alignments) The best set is below. Score: 48197.2, number of alignments: 2, unaligned bases: 0 This contig is misassembled. Real Alignment 1: 43683 54962 | 11280 1 | 11280 11280 | 99.97 | meta_ref_1_Lactobacillus_plantarum_22_55k.fa NODE_6_length_49658_cov_2.58581 Extensive misassembly (relocation, inconsistency = 1200) between these two alignments Real Alignment 2: 5305 42482 | 48458 11281 | 37178 37178 | 99.98 | meta_ref_1_Lactobacillus_plantarum_22_55k.fa NODE_6_length_49658_cov_2.58581 CONTIG: NODE_8_length_182_cov_2.81102 (1152bp) Best alignment score: 284.0 (LEN: 285, IDY: 99.65), Total number of alignments: 2 Looking for the best set of alignments (out of 2 total alignments) The best set is below. Score: 409.0, number of alignments: 2, unaligned bases: 685 This contig is misassembled. This contig is partially unaligned. (Aligned 467 out of 1132 non-N bases (41.25%)) Warning! This contig is more unaligned than misassembled. Contig length is 1152 (number of Ns: 20) and total length of all aligns is 467 Alignment: 3645 3826 | 182 1 | 182 182 | 100.0 | meta_ref_1_Lactobacillus_plantarum_22_55k.fa NODE_8_length_182_cov_2.81102 Alignment: 3112 3396 | 868 1152 | 285 285 | 99.65 | meta_ref_1_Lactobacillus_plantarum_22_55k.fa NODE_8_length_182_cov_2.81102 Unaligned bases: 685 CONTIG: NODE_18_length_714_cov_2.71927 (714bp) Best alignment score: 713.0 (LEN: 714, IDY: 99.86), Total number of alignments: 1 One align captures most of this contig: 78 791 | 1 714 | 714 714 | 99.86 | meta_ref_1_Lactobacillus_plantarum_22_55k.fa NODE_18_length_714_cov_2.71927 Analyzing coverage... Writing SNPs into /home/psgendb/BIRCHDEV/install/quast-5.2.0/quast_test_output/runs_per_reference/meta_ref_1/contigs_reports/minimap_output/meta_contigs_1.used_snps Analysis is finished! Founded SNPs were written into /home/psgendb/BIRCHDEV/install/quast-5.2.0/quast_test_output/runs_per_reference/meta_ref_1/contigs_reports/minimap_output/meta_contigs_1.used_snps Results: Local Misassemblies: 0 Misassemblies: 1 Relocations: 1 Translocations: 0 Inversions: 0 Scaffold gap extensive misassemblies: 0 Scaffold gap local misassemblies: 0 Misassembled Contigs: 1 Misassembled Contig Bases: 48458 Misassemblies Inter-Contig Overlap ("Extra" Aligned Bases): 0 Unaligned Contigs: 0 + 1 part Half Unaligned Contigs with Misassemblies: 1 Unaligned Bases in Fully and Partially Unaligned Contigs: 665 Ambiguously Mapped Contigs: 0 Total Bases in Ambiguously Mapped Contigs: 0 "Extra" Aligned Bases in Ambiguously Mapped Contigs: 0 Note that --allow-ambiguity option was set to "one" and only first alignment per each of these contigs was used (there is no "extra" aligned bases). Covered Bases in Reference: 49639 Raw Aligned Bases in Assembly: 49639 Total Aligned Bases in Assembly (with "Extras"): 49639 SNPs: 13 Insertions: 0 Deletions: 0