Aligning contigs to reference... Aligning contigs to the reference Filtering alignments... Parsing coords... Loading reference... Loaded [meta_ref_1_Lactobacillus_plantarum_22_55k.fa] Total Regions: 1 Total Region Length: 54999 Analyzing contigs... CONTIG: NODE_2_length_49658_cov_2.58581_ID_2497514 (49658bp) Best alignment score: 49648.1 (LEN: 49658, IDY: 99.98), Total number of alignments: 1 One align captures most of this contig: 5305 54962 | 1 49658 | 49658 49658 | 99.98 | meta_ref_1_Lactobacillus_plantarum_22_55k.fa NODE_2_length_49658_cov_2.58581_ID_2497514 CONTIG: NODE_10_length_714_cov_2.71927_ID_9333852 (714bp) Best alignment score: 713.0 (LEN: 714, IDY: 99.86), Total number of alignments: 1 One align captures most of this contig: 78 791 | 714 1 | 714 714 | 99.86 | meta_ref_1_Lactobacillus_plantarum_22_55k.fa NODE_10_length_714_cov_2.71927_ID_9333852 CONTIG: NODE_29_length_219_cov_25.5122_ID_8985538 (763bp) Best alignment score: 197.4 (LEN: 219, IDY: 90.13), Total number of alignments: 2 Looking for the best set of alignments (out of 2 total alignments) The best set is below. Score: 330.4, number of alignments: 2, unaligned bases: 361 This contig is misassembled. Real Alignment 1: 4643 4861 | 1 219 | 219 219 | 90.13 | meta_ref_1_Lactobacillus_plantarum_22_55k.fa NODE_29_length_219_cov_25.5122_ID_8985538 Extensive misassembly (relocation, inconsistency = -1425) between these two alignments Real Alignment 2: 3645 3827 | 428 610 | 183 183 | 100.0 | meta_ref_1_Lactobacillus_plantarum_22_55k.fa NODE_29_length_219_cov_25.5122_ID_8985538 Analyzing coverage... Writing SNPs into /home/psgendb/BIRCHDEV/install/quast-5.2.0/quast_test_output/runs_per_reference/meta_ref_1/contigs_reports/minimap_output/meta_contigs_2.used_snps Analysis is finished! Founded SNPs were written into /home/psgendb/BIRCHDEV/install/quast-5.2.0/quast_test_output/runs_per_reference/meta_ref_1/contigs_reports/minimap_output/meta_contigs_2.used_snps Results: Local Misassemblies: 0 Misassemblies: 1 Relocations: 1 Translocations: 0 Inversions: 0 Scaffold gap extensive misassemblies: 0 Scaffold gap local misassemblies: 0 Misassembled Contigs: 1 Misassembled Contig Bases: 763 Misassemblies Inter-Contig Overlap ("Extra" Aligned Bases): 0 Unaligned Contigs: 0 + 0 part Half Unaligned Contigs with Misassemblies: 0 Unaligned Bases in Fully and Partially Unaligned Contigs: 0 Ambiguously Mapped Contigs: 0 Total Bases in Ambiguously Mapped Contigs: 0 "Extra" Aligned Bases in Ambiguously Mapped Contigs: 0 Note that --allow-ambiguity option was set to "one" and only first alignment per each of these contigs was used (there is no "extra" aligned bases). Covered Bases in Reference: 50774 Raw Aligned Bases in Assembly: 50774 Total Aligned Bases in Assembly (with "Extras"): 50774 SNPs: 27 Insertions: 4 Deletions: 4