#!/usr/bin/env python ################################################ # SORTS THE SEQUENCES IN A FASTA FILE BY NAME # # THIS IS OPEN SOURCE CODE (YAY!) # ################################################ # LICENSE # This code is licensed under the Creative Commons 3.0 # Attribution + ShareAlike license - for details see: # http://creativecommons.org/licenses/by-sa/3.0/ # # DATE: February 27th, 2014 # # PROGRAM AUTHOR (PROGRAMMER) # Graham Alvare - home.cc.umanitoba.ca/~alvare # # CO-AUTHORS/ACKNOWLEDGEMENTS # Justin Zhang - for providing me information on the SAM format, # and test data to build and debug this program. # Dr. Brian Fristensky - my work supervisor, and the man who introduced # me to the wonderful field of bioinformatics. # # QUESTIONS & COMMENTS # If you have any questions, please contact me: alvare@cc.umanitoba.ca # I usually get back to people within 1-2 weekdays (weekends, I am slower) # # P.S. Please also let me know of any bugs, or if you have any suggestions. # I am generally happy to help create new tools, or modify my existing # tools to make them more useful. # # Happy usage! import sys, os, os.path, re, collections from Bio import SeqIO if __name__=="__main__": if len(sys.argv) > 1: for filename in sys.argv[1:]: sequences = dict() # Print the FASTA file header print "File: " + filename # Open the FASTA file and parse each sequence for record in SeqIO.parse(filename, 'fasta'): if record.id in sequences: print " Skipping duplicate sequence " + record.id else: sequences[record.id] = record.seq outname = os.path.splitext(filename)[0] + ".sorted.fa" outfile = open(outname, 'w') for key in sorted(sequences, key=lambda k: k.lower()): print >> outfile, "> " + str(key) print >> outfile, str(sequences[key]) outfile.close() else: print "Usage: fsa_len.py " print "Output: the length of each sequence in each FASTA file"